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The map label for this gene is cofE [H]

Identifier: 169630688

GI number: 169630688

Start: 3658268

End: 3659644

Strand: Direct

Name: cofE [H]

Synonym: MAB_3608

Alternate gene names: 169630688

Gene position: 3658268-3659644 (Clockwise)

Preceding gene: 169630687

Following gene: 169630689

Centisome position: 72.2

GC content: 64.78

Gene sequence:

>1377_bases
ATGACCCCACCTGCGGATTCTCCTGCACCGGAACATGGTTCGGCAGCTCCGGTTCAAATCCTACCCGTAACCGGCCTGCC
CGAATTCCGCCCGGGCGACGACGTGGCCGCGGCCATCGCAGGCGCGGCGCCATGGCTGCGTGACGGTGATGTGATCGTCA
TCACCAGCAAGATAATCTCCAAGGCCGAGGGACGGATCGTCGCCGCGCCCACTGATCCCGAAGCCCGGGACACACTGCGG
CGCAAGTTAATCGACTCCGAATCGATCCGCGTGTTGGCCCGCAAGGGTAAGACACTGATCACCGAGAACACCATCGGCAT
CGTGCAGGCCGCCGCGGGAATCGACGCGTCAAACGTCGACACCGCCGAACTGGTTCTGCTTCCCACCGACCCGGACGCCA
GCGCGGCGGCAGTGCGGCTGGCGCTGTCACAACGCTTGGGCGTCAAGGTTGCGGTGGTGATCACCGACACCATGGGACGA
GCCTGGCGCAACGGCCAAACTGATGCCGCCATCGGCTCCGCCGGCATACCAGTCCTCTACGGTTATGCGGGCGCAAAAGA
CAAACACGGTAACGAACTTCAGGTCACCGAGGTCGCCATCGCCGACGAAATCTCGGCTGCCGCCGACTTGGTCAAGGGCA
AGCTGACCGATGTACCGGTCGCGGTGGTGCGCGGCCTGACCCTGCTCGATGACGGCAGTGCGGCGAGCGACCTGTTGCGT
TCCGGACCGGACGACTTGTTCTGGCTCGGCACCAACGAGGCACTGGAGCAAGGACGGCGCGAAGCAATACTCGTGCGCCG
CTCCATCCGCCAATTCGCAGATATCCCAGTCGATCCCGATCTGCTGCGGGAAGCCATCGGTGAAGCGCTGACCGCACCCG
CACCGCATCACACACATCCGGTCCGATTCGTGTGGGTGCGCGACCTCACGACGCGGCGTGCGCTCCTGGACGCCCTGAAA
CAGGCCTGGGCCGCCGATCTCACCGCCGACGGCCGTAGCCCGGAATCAGTGCAGAAGAGGGTGGCGCGGGGACAAATCCT
TTATGACGCACCCGAAATCGTGATCCCGTTCCTAGTACCTGATGGCGCGCACGACTATCCGGACGACCGCCGAACCGCCG
CGGAGCGCACTATGTTCACCGTCGCGGTTGGCGCGGCGGTGCAGGCTCTTCTGGTCGCCCTCGCCGCCCGGCAGGTCGGC
AGCTGCTGGATCGGATCCACCATCTTCGCGGGCGACGTTGTACGCAAGCAGCTGGTAGTGGATTCTTCGTGGGAACCGAT
GGGCGCCATAGCCATCGGCTATCCGCATGGCTATCCCGAGGAAGGCCTGGACCCACGCACTCCCCTGCCGCCGGGACAGA
TGCTGGTGGAATTGTGA

Upstream 100 bases:

>100_bases
TTGCGGGAGTTCAGGTACGGTCGGTTCCGCTACTCATGACCGACCCCGACGCGACGGCGGCAATGGTACGTGCGGGACTT
GACCTGGCCGGAGTTACCTT

Downstream 100 bases:

>100_bases
CGGCCCGGCACCCCGGTGAGGGCCATCAGATGCCCTCGGATCTGCGTGAGTCCGCTATCGAGCTGCTCTCCTCATGGCAG
GCGCCCGATGCAGAACAAGA

Product: F420-0--gamma-glutamyl ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MTPPADSPAPEHGSAAPVQILPVTGLPEFRPGDDVAAAIAGAAPWLRDGDVIVITSKIISKAEGRIVAAPTDPEARDTLR
RKLIDSESIRVLARKGKTLITENTIGIVQAAAGIDASNVDTAELVLLPTDPDASAAAVRLALSQRLGVKVAVVITDTMGR
AWRNGQTDAAIGSAGIPVLYGYAGAKDKHGNELQVTEVAIADEISAAADLVKGKLTDVPVAVVRGLTLLDDGSAASDLLR
SGPDDLFWLGTNEALEQGRREAILVRRSIRQFADIPVDPDLLREAIGEALTAPAPHHTHPVRFVWVRDLTTRRALLDALK
QAWAADLTADGRSPESVQKRVARGQILYDAPEIVIPFLVPDGAHDYPDDRRTAAERTMFTVAVGAAVQALLVALAARQVG
SCWIGSTIFAGDVVRKQLVVDSSWEPMGAIAIGYPHGYPEEGLDPRTPLPPGQMLVEL

Sequences:

>Translated_458_residues
MTPPADSPAPEHGSAAPVQILPVTGLPEFRPGDDVAAAIAGAAPWLRDGDVIVITSKIISKAEGRIVAAPTDPEARDTLR
RKLIDSESIRVLARKGKTLITENTIGIVQAAAGIDASNVDTAELVLLPTDPDASAAAVRLALSQRLGVKVAVVITDTMGR
AWRNGQTDAAIGSAGIPVLYGYAGAKDKHGNELQVTEVAIADEISAAADLVKGKLTDVPVAVVRGLTLLDDGSAASDLLR
SGPDDLFWLGTNEALEQGRREAILVRRSIRQFADIPVDPDLLREAIGEALTAPAPHHTHPVRFVWVRDLTTRRALLDALK
QAWAADLTADGRSPESVQKRVARGQILYDAPEIVIPFLVPDGAHDYPDDRRTAAERTMFTVAVGAAVQALLVALAARQVG
SCWIGSTIFAGDVVRKQLVVDSSWEPMGAIAIGYPHGYPEEGLDPRTPLPPGQMLVEL
>Mature_457_residues
TPPADSPAPEHGSAAPVQILPVTGLPEFRPGDDVAAAIAGAAPWLRDGDVIVITSKIISKAEGRIVAAPTDPEARDTLRR
KLIDSESIRVLARKGKTLITENTIGIVQAAAGIDASNVDTAELVLLPTDPDASAAAVRLALSQRLGVKVAVVITDTMGRA
WRNGQTDAAIGSAGIPVLYGYAGAKDKHGNELQVTEVAIADEISAAADLVKGKLTDVPVAVVRGLTLLDDGSAASDLLRS
GPDDLFWLGTNEALEQGRREAILVRRSIRQFADIPVDPDLLREAIGEALTAPAPHHTHPVRFVWVRDLTTRRALLDALKQ
AWAADLTADGRSPESVQKRVARGQILYDAPEIVIPFLVPDGAHDYPDDRRTAAERTMFTVAVGAAVQALLVALAARQVGS
CWIGSTIFAGDVVRKQLVVDSSWEPMGAIAIGYPHGYPEEGLDPRTPLPPGQMLVEL

Specific function: Catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives [H]

COG id: COG1478

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the CofE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002847
- InterPro:   IPR008225
- InterPro:   IPR019943
- InterPro:   IPR000415 [H]

Pfam domain/function: PF01996 F420_ligase; PF00881 Nitroreductase [H]

EC number: NA

Molecular weight: Translated: 48524; Mature: 48393

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPPADSPAPEHGSAAPVQILPVTGLPEFRPGDDVAAAIAGAAPWLRDGDVIVITSKIIS
CCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCEEEEEHHHHH
KAEGRIVAAPTDPEARDTLRRKLIDSESIRVLARKGKTLITENTIGIVQAAAGIDASNVD
HCCCCEEECCCCCHHHHHHHHHHCCCHHHHHHHHCCCEEEEECCHHHHHHHCCCCCCCCC
TAELVLLPTDPDASAAAVRLALSQRLGVKVAVVITDTMGRAWRNGQTDAAIGSAGIPVLY
CEEEEEEECCCCCHHHHHHHHHHHHCCEEEEEEEECCHHHHHCCCCCCCCCCCCCCCEEE
GYAGAKDKHGNELQVTEVAIADEISAAADLVKGKLTDVPVAVVRGLTLLDDGSAASDLLR
ECCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEECCCCHHHHHHH
SGPDDLFWLGTNEALEQGRREAILVRRSIRQFADIPVDPDLLREAIGEALTAPAPHHTHP
CCCCCEEEECCHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCC
VRFVWVRDLTTRRALLDALKQAWAADLTADGRSPESVQKRVARGQILYDAPEIVIPFLVP
EEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEECCCEEEEEEEC
DGAHDYPDDRRTAAERTMFTVAVGAAVQALLVALAARQVGSCWIGSTIFAGDVVRKQLVV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHC
DSSWEPMGAIAIGYPHGYPEEGLDPRTPLPPGQMLVEL
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHEEEC
>Mature Secondary Structure 
TPPADSPAPEHGSAAPVQILPVTGLPEFRPGDDVAAAIAGAAPWLRDGDVIVITSKIIS
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCEEEEEHHHHH
KAEGRIVAAPTDPEARDTLRRKLIDSESIRVLARKGKTLITENTIGIVQAAAGIDASNVD
HCCCCEEECCCCCHHHHHHHHHHCCCHHHHHHHHCCCEEEEECCHHHHHHHCCCCCCCCC
TAELVLLPTDPDASAAAVRLALSQRLGVKVAVVITDTMGRAWRNGQTDAAIGSAGIPVLY
CEEEEEEECCCCCHHHHHHHHHHHHCCEEEEEEEECCHHHHHCCCCCCCCCCCCCCCEEE
GYAGAKDKHGNELQVTEVAIADEISAAADLVKGKLTDVPVAVVRGLTLLDDGSAASDLLR
ECCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEECCCCHHHHHHH
SGPDDLFWLGTNEALEQGRREAILVRRSIRQFADIPVDPDLLREAIGEALTAPAPHHTHP
CCCCCEEEECCHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCC
VRFVWVRDLTTRRALLDALKQAWAADLTADGRSPESVQKRVARGQILYDAPEIVIPFLVP
EEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEECCCEEEEEEEC
DGAHDYPDDRRTAAERTMFTVAVGAAVQALLVALAARQVGSCWIGSTIFAGDVVRKQLVV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHC
DSSWEPMGAIAIGYPHGYPEEGLDPRTPLPPGQMLVEL
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA