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The map label for this gene is manA [H]

Identifier: 169630681

GI number: 169630681

Start: 3651693

End: 3652910

Strand: Reverse

Name: manA [H]

Synonym: MAB_3601c

Alternate gene names: 169630681

Gene position: 3652910-3651693 (Counterclockwise)

Preceding gene: 169630682

Following gene: 169630680

Centisome position: 72.09

GC content: 66.75

Gene sequence:

>1218_bases
GTGAATCTGCTCAGGGGGGCGATAAGGACGTACGCCTGGGGTTCGCGGACGGCCATCGCCGAGTTCACCGGACGCCCCAC
GCCCACACCGCATCCGGAGGCCGAGCTGTGGCTGGGGGCGCATCCCGCGGACCCCGCATATCTGGAAACCGGTGGCGGCG
CAGAGTCGTTGCTTGAGGTGGTGGCCGCCGATCCGACCGGCCAGCTGGGTGCGGCGTCCGTGGCGGAGTTTGGTGACCAG
TTACCCTTCCTGCTCAAGGTGCTAGCCGCCGACGAGCCACTTTCCTTGCAGGCGCACCCAAGCGCCCAGCAGGCCGCGGA
CGGGTTCGCGCGCGAAGAGGCGGCGGGTGTGCCGCTCAACTCTCCGGTACGTAACTACCGCGATCGCAACCACAAACCGG
AACTTGTGGTGGCCTTAGACCGTTTCGAGGCGCTGGCGGGTTTCCGTGATCCGGGCGATACGGTGGACCTGTTCCGTGCG
CTCGACGTCGAAGCGCTGACACCGTATGTGAACCTGCTGGCCGGTCAATCCGATGCCGATGGTCTACGGGCCCTGTTCAC
CACCTGGATCACCCTTCCCCAGCCGAGTCTGGATGTGTTGGTGCCCGCGGTGCTCGACGGGGCGGTGCGTTATCTGTCCT
CCGATGACGTGCGCTTCGTCGGCGAGGCCCGCACGCTCCTGGAGTTGGGTGAGCGCTATCCCGCAGACGCGGGCGTGCTG
GCCTCGCTGCTGTTGAATCGGCTGACGCTGGAACCGGGGGAGGGGATCTACCTGCCTGCCGGGAACCTGCACGCGTATCT
GCGCGGCTTGGCGGTGGAGATCATGGCCAACTCCGATAACGTCCTGCGCGGCGGCCTGACGCCCAAGCACGTAGATGTTC
CGGAACTGCTTCGGGTGCTGGACTTTTCGCCCGCTTCCGAGGCGCACCTACATGTCGAGACGGTTACCGATGGTGCCCAG
ACCCGCTACCGCACTCCTGCCAGGGAGTTCGCGCTTTCGCGTTTCGACTTGGCGGCCGGCGAGCCCACCCAGGTGGGTAT
CAAGGGCCCGCGGATACTGCTGTGCACCCAGGGCGATGTGACTCTGGACGGTTCGGGGGCCGGCCTGAAGGTGCGTGCGG
GTCAGTCGGTGTGGGTGCCGGCCGACGGGGGCAGTGTCACACTGACCGGGCGGTCCGATGCCCGGGTGTTCATGGCAACA
GTCGACGCGGACGCGTAA

Upstream 100 bases:

>100_bases
TAGCGGCCAGGCCTTCGGAGCTGGCACAGTTGATGATGCTTTCGACACGGCTCGAAATGGCCTCGGTGTACGCCCGGCTT
GGTGGGGGGAGATCCGGGCT

Downstream 100 bases:

>100_bases
CGGCCGCAGGGGCGGGAACAGAAGGAGAGTCGATGTCGGCAGGTGGCAGTAAGAGGGCGATTATCGCGGCGCTGGCGGCC
AATGCCGGTATCGCCGTGGC

Product: mannose-6-phosphate isomerase ManA

Products: NA

Alternate protein names: Phosphohexomutase; Phosphomannose isomerase; PMI [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MNLLRGAIRTYAWGSRTAIAEFTGRPTPTPHPEAELWLGAHPADPAYLETGGGAESLLEVVAADPTGQLGAASVAEFGDQ
LPFLLKVLAADEPLSLQAHPSAQQAADGFAREEAAGVPLNSPVRNYRDRNHKPELVVALDRFEALAGFRDPGDTVDLFRA
LDVEALTPYVNLLAGQSDADGLRALFTTWITLPQPSLDVLVPAVLDGAVRYLSSDDVRFVGEARTLLELGERYPADAGVL
ASLLLNRLTLEPGEGIYLPAGNLHAYLRGLAVEIMANSDNVLRGGLTPKHVDVPELLRVLDFSPASEAHLHVETVTDGAQ
TRYRTPAREFALSRFDLAAGEPTQVGIKGPRILLCTQGDVTLDGSGAGLKVRAGQSVWVPADGGSVTLTGRSDARVFMAT
VDADA

Sequences:

>Translated_405_residues
MNLLRGAIRTYAWGSRTAIAEFTGRPTPTPHPEAELWLGAHPADPAYLETGGGAESLLEVVAADPTGQLGAASVAEFGDQ
LPFLLKVLAADEPLSLQAHPSAQQAADGFAREEAAGVPLNSPVRNYRDRNHKPELVVALDRFEALAGFRDPGDTVDLFRA
LDVEALTPYVNLLAGQSDADGLRALFTTWITLPQPSLDVLVPAVLDGAVRYLSSDDVRFVGEARTLLELGERYPADAGVL
ASLLLNRLTLEPGEGIYLPAGNLHAYLRGLAVEIMANSDNVLRGGLTPKHVDVPELLRVLDFSPASEAHLHVETVTDGAQ
TRYRTPAREFALSRFDLAAGEPTQVGIKGPRILLCTQGDVTLDGSGAGLKVRAGQSVWVPADGGSVTLTGRSDARVFMAT
VDADA
>Mature_405_residues
MNLLRGAIRTYAWGSRTAIAEFTGRPTPTPHPEAELWLGAHPADPAYLETGGGAESLLEVVAADPTGQLGAASVAEFGDQ
LPFLLKVLAADEPLSLQAHPSAQQAADGFAREEAAGVPLNSPVRNYRDRNHKPELVVALDRFEALAGFRDPGDTVDLFRA
LDVEALTPYVNLLAGQSDADGLRALFTTWITLPQPSLDVLVPAVLDGAVRYLSSDDVRFVGEARTLLELGERYPADAGVL
ASLLLNRLTLEPGEGIYLPAGNLHAYLRGLAVEIMANSDNVLRGGLTPKHVDVPELLRVLDFSPASEAHLHVETVTDGAQ
TRYRTPAREFALSRFDLAAGEPTQVGIKGPRILLCTQGDVTLDGSGAGLKVRAGQSVWVPADGGSVTLTGRSDARVFMAT
VDADA

Specific function: Involved in the conversion of glucose to GDP-L-fucose, which can be converted to L-fucose, a capsular polysaccharide [H]

COG id: COG1482

COG function: function code G; Phosphomannose isomerase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 1 family [H]

Homologues:

Organism=Homo sapiens, GI4505235, Length=427, Percent_Identity=31.1475409836066, Blast_Score=175, Evalue=6e-44,
Organism=Escherichia coli, GI1787899, Length=399, Percent_Identity=39.0977443609023, Blast_Score=273, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI71997620, Length=315, Percent_Identity=32.3809523809524, Blast_Score=140, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI17557650, Length=365, Percent_Identity=30.4109589041096, Blast_Score=133, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6320839, Length=426, Percent_Identity=29.3427230046948, Blast_Score=165, Evalue=1e-41,
Organism=Drosophila melanogaster, GI21356061, Length=415, Percent_Identity=27.710843373494, Blast_Score=143, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR001250
- InterPro:   IPR016305
- InterPro:   IPR018050
- InterPro:   IPR014710 [H]

Pfam domain/function: PF01238 PMI_typeI [H]

EC number: =5.3.1.8 [H]

Molecular weight: Translated: 43005; Mature: 43005

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00965 PMI_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLRGAIRTYAWGSRTAIAEFTGRPTPTPHPEAELWLGAHPADPAYLETGGGAESLLEV
CCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCEEECCCCHHHHHHH
VAADPTGQLGAASVAEFGDQLPFLLKVLAADEPLSLQAHPSAQQAADGFAREEAAGVPLN
HHCCCCCCCCHHHHHHHCCCHHHHHHHHHCCCCCEEECCCCHHHHHCCHHHHHHCCCCCC
SPVRNYRDRNHKPELVVALDRFEALAGFRDPGDTVDLFRALDVEALTPYVNLLAGQSDAD
CHHHHHHCCCCCCCEEEEEHHHHHHHCCCCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCH
GLRALFTTWITLPQPSLDVLVPAVLDGAVRYLSSDDVRFVGEARTLLELGERYPADAGVL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCHHHH
ASLLLNRLTLEPGEGIYLPAGNLHAYLRGLAVEIMANSDNVLRGGLTPKHVDVPELLRVL
HHHHHHHHCCCCCCEEEECCCCHHHHHHCEEEEEEECCCCEEECCCCCCCCCHHHHHHHH
DFSPASEAHLHVETVTDGAQTRYRTPAREFALSRFDLAAGEPTQVGIKGPRILLCTQGDV
CCCCCCCCEEEEEEECCCCHHHCCCCHHHHHHHHHHCCCCCCCEECCCCCEEEEECCCCE
TLDGSGAGLKVRAGQSVWVPADGGSVTLTGRSDARVFMATVDADA
EECCCCCCEEEECCCEEEEECCCCEEEEECCCCCEEEEEEECCCC
>Mature Secondary Structure
MNLLRGAIRTYAWGSRTAIAEFTGRPTPTPHPEAELWLGAHPADPAYLETGGGAESLLEV
CCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCEEECCCCHHHHHHH
VAADPTGQLGAASVAEFGDQLPFLLKVLAADEPLSLQAHPSAQQAADGFAREEAAGVPLN
HHCCCCCCCCHHHHHHHCCCHHHHHHHHHCCCCCEEECCCCHHHHHCCHHHHHHCCCCCC
SPVRNYRDRNHKPELVVALDRFEALAGFRDPGDTVDLFRALDVEALTPYVNLLAGQSDAD
CHHHHHHCCCCCCCEEEEEHHHHHHHCCCCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCH
GLRALFTTWITLPQPSLDVLVPAVLDGAVRYLSSDDVRFVGEARTLLELGERYPADAGVL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCHHHH
ASLLLNRLTLEPGEGIYLPAGNLHAYLRGLAVEIMANSDNVLRGGLTPKHVDVPELLRVL
HHHHHHHHCCCCCCEEEECCCCHHHHHHCEEEEEEECCCCEEECCCCCCCCCHHHHHHHH
DFSPASEAHLHVETVTDGAQTRYRTPAREFALSRFDLAAGEPTQVGIKGPRILLCTQGDV
CCCCCCCCEEEEEEECCCCHHHCCCCHHHHHHHHHHCCCCCCCEECCCCCEEEEECCCCE
TLDGSGAGLKVRAGQSVWVPADGGSVTLTGRSDARVFMATVDADA
EECCCCCCEEEECCCEEEEECCCCEEEEECCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6397402; 9097039; 9278503 [H]