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The map label for this gene is menF [H]

Identifier: 169630616

GI number: 169630616

Start: 3581822

End: 3582928

Strand: Direct

Name: menF [H]

Synonym: MAB_3535

Alternate gene names: 169630616

Gene position: 3581822-3582928 (Clockwise)

Preceding gene: 169630615

Following gene: 169630617

Centisome position: 70.69

GC content: 65.49

Gene sequence:

>1107_bases
ATGACGGCCACCGACTCTCCCGCCCGCCCACATTTCGTGATGTCGCGAGCCGCCGACGCGCTGTACGCGTATGGCCGCGA
TGCGACCTATGACGACGTCTTCACCGCGGCGGAAGCCTTGCGCACCGGCCGTCATCACATGTTGGTGGGTGCCCTGCCGT
TCGACACCTCCCGTCCCGTCGCACTGACGGCTCCAGTCACGGTCCAACGCGGACTGCCCCAAGAGTTCTACGGCGCAATG
CCGAATACTCAGGTGACACAACAGGTACCGGCGCCCTCTGAGCATCTGCGACGGGTCACCGAGGCGGTCGAGGTGCTGCG
CAGCGACGCAGGGCTGGCCAAGGTTGTCTTGGCGCGGGTGTTGGAGCTGCGGGCCGAGTCGCCCGTGGACCCATTGGTGC
TGACACACCGATTGATTCGAAACGACCCCGACGCCAACAGCTTTTGCGTGGACCTCTCCCCCGCCGGCAACCGATTCCGC
GGACACACCATGGTGGGATGCAGCCCAGAATTGCTGGTGGAACGCCGAGGCGACATGGTGATCTGTCATCCCTTCGCCGG
TTCCGCACCGCGGTCCAGTGACCCATCCCAGGATCGGCGCACGGGCGAAGATCTAGCGGGTTCACACAAGAATCGCCACG
AGCACTCCGTCGTGATCGACCAGCTACGCGAGGATCTCGCGTCCCTGTGCGTTGATGTCCAGGTCCCCCCGGAGCCGACG
TTGAGCCGCACCGCGGCACTCTGGCACCTGAGCACGTCCATCAGCGCCCGCCTCCGCCAAAGTTCAACCACAGCACTCGA
TTTGGCCTTGGCGCTGCACCCCACGCCCGCCGTGTGCGGCACCCCGACCGAGGCCGCTGCCGACCTGATCTCACGCATTG
AGGGTGATCGCGGTTTCTACGCGGGTGCGATCGGCTGGTGTGACCGCAATGGGGACGGCCGCTGGGCCGTCACCATCCGC
TGCGCCGAACTGTCGGCAGACGGCACCGCGATTCGCGCCTACGCCGGCGGCGGCCTCGTCGCCGAATCGGACCCGCAGGA
CGAACTTTCAGAGACCACAGTCAAATTCCGGACCGTGTTGGCAGCGCTAGGAGCCAAAATTGACTAA

Upstream 100 bases:

>100_bases
GCCACCGCTTCGATCGCTATCGGCGGCCACGAGCACGGCCAGCGCCAGGTCGCCGCACTTGGCCTAACCGGGTACCAGCA
CCCGGTACCGTCAAGCTGAC

Downstream 100 bases:

>100_bases
CACCCTTATTCGCCGCGCCACCGAAGCCGACATCCCGGCCATTGTCGGGCTCATCGAGGACCTCGCGGAGTACGAGCACG
CCCGCGATGAGTGCCTCATC

Product: isochorismate synthase EntC

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MTATDSPARPHFVMSRAADALYAYGRDATYDDVFTAAEALRTGRHHMLVGALPFDTSRPVALTAPVTVQRGLPQEFYGAM
PNTQVTQQVPAPSEHLRRVTEAVEVLRSDAGLAKVVLARVLELRAESPVDPLVLTHRLIRNDPDANSFCVDLSPAGNRFR
GHTMVGCSPELLVERRGDMVICHPFAGSAPRSSDPSQDRRTGEDLAGSHKNRHEHSVVIDQLREDLASLCVDVQVPPEPT
LSRTAALWHLSTSISARLRQSSTTALDLALALHPTPAVCGTPTEAAADLISRIEGDRGFYAGAIGWCDRNGDGRWAVTIR
CAELSADGTAIRAYAGGGLVAESDPQDELSETTVKFRTVLAALGAKID

Sequences:

>Translated_368_residues
MTATDSPARPHFVMSRAADALYAYGRDATYDDVFTAAEALRTGRHHMLVGALPFDTSRPVALTAPVTVQRGLPQEFYGAM
PNTQVTQQVPAPSEHLRRVTEAVEVLRSDAGLAKVVLARVLELRAESPVDPLVLTHRLIRNDPDANSFCVDLSPAGNRFR
GHTMVGCSPELLVERRGDMVICHPFAGSAPRSSDPSQDRRTGEDLAGSHKNRHEHSVVIDQLREDLASLCVDVQVPPEPT
LSRTAALWHLSTSISARLRQSSTTALDLALALHPTPAVCGTPTEAAADLISRIEGDRGFYAGAIGWCDRNGDGRWAVTIR
CAELSADGTAIRAYAGGGLVAESDPQDELSETTVKFRTVLAALGAKID
>Mature_367_residues
TATDSPARPHFVMSRAADALYAYGRDATYDDVFTAAEALRTGRHHMLVGALPFDTSRPVALTAPVTVQRGLPQEFYGAMP
NTQVTQQVPAPSEHLRRVTEAVEVLRSDAGLAKVVLARVLELRAESPVDPLVLTHRLIRNDPDANSFCVDLSPAGNRFRG
HTMVGCSPELLVERRGDMVICHPFAGSAPRSSDPSQDRRTGEDLAGSHKNRHEHSVVIDQLREDLASLCVDVQVPPEPTL
SRTAALWHLSTSISARLRQSSTTALDLALALHPTPAVCGTPTEAAADLISRIEGDRGFYAGAIGWCDRNGDGRWAVTIRC
AELSADGTAIRAYAGGGLVAESDPQDELSETTVKFRTVLAALGAKID

Specific function: Might be involved in the biosynthesis of menaquinone. Converts chorismate to isochorismate [H]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786809, Length=329, Percent_Identity=35.8662613981763, Blast_Score=195, Evalue=4e-51,
Organism=Escherichia coli, GI87082077, Length=261, Percent_Identity=30.6513409961686, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788114, Length=209, Percent_Identity=30.622009569378, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1787518, Length=245, Percent_Identity=31.4285714285714, Blast_Score=80, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320935, Length=197, Percent_Identity=33.502538071066, Blast_Score=96, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 39571; Mature: 39440

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATDSPARPHFVMSRAADALYAYGRDATYDDVFTAAEALRTGRHHMLVGALPFDTSRPV
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCE
ALTAPVTVQRGLPQEFYGAMPNTQVTQQVPAPSEHLRRVTEAVEVLRSDAGLAKVVLARV
EEECCCHHHCCCCHHHHCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LELRAESPVDPLVLTHRLIRNDPDANSFCVDLSPAGNRFRGHTMVGCSPELLVERRGDMV
HHHHCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCEEECCCHHHHHHCCCCEE
ICHPFAGSAPRSSDPSQDRRTGEDLAGSHKNRHEHSVVIDQLREDLASLCVDVQVPPEPT
EEECCCCCCCCCCCCCHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCC
LSRTAALWHLSTSISARLRQSSTTALDLALALHPTPAVCGTPTEAAADLISRIEGDRGFY
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEE
AGAIGWCDRNGDGRWAVTIRCAELSADGTAIRAYAGGGLVAESDPQDELSETTVKFRTVL
ECEEEEEECCCCCEEEEEEEEEEECCCCCEEEEEECCCEEECCCCHHHHHHHHHHHHHHH
AALGAKID
HHHCCCCC
>Mature Secondary Structure 
TATDSPARPHFVMSRAADALYAYGRDATYDDVFTAAEALRTGRHHMLVGALPFDTSRPV
CCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCE
ALTAPVTVQRGLPQEFYGAMPNTQVTQQVPAPSEHLRRVTEAVEVLRSDAGLAKVVLARV
EEECCCHHHCCCCHHHHCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LELRAESPVDPLVLTHRLIRNDPDANSFCVDLSPAGNRFRGHTMVGCSPELLVERRGDMV
HHHHCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCEEECCCHHHHHHCCCCEE
ICHPFAGSAPRSSDPSQDRRTGEDLAGSHKNRHEHSVVIDQLREDLASLCVDVQVPPEPT
EEECCCCCCCCCCCCCHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCC
LSRTAALWHLSTSISARLRQSSTTALDLALALHPTPAVCGTPTEAAADLISRIEGDRGFY
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEE
AGAIGWCDRNGDGRWAVTIRCAELSADGTAIRAYAGGGLVAESDPQDELSETTVKFRTVL
ECEEEEEECCCCCEEEEEEEEEEECCCCCEEEEEECCCEEECCCCHHHHHHHHHHHHHHH
AALGAKID
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]