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The map label for this gene is nadE

Identifier: 169630500

GI number: 169630500

Start: 3465504

End: 3466325

Strand: Direct

Name: nadE

Synonym: MAB_3419

Alternate gene names: 169630500

Gene position: 3465504-3466325 (Clockwise)

Preceding gene: 169630499

Following gene: 169630502

Centisome position: 68.39

GC content: 64.96

Gene sequence:

>822_bases
ATGAGCAGCCTTCGCGAAGAGATCCGGTCCGCCCTCGACGTCTCCCCCACGATCGATCCGGCGGCCGAGGTGTCACGACG
GGTGGGCTTCCTCAAGGATTATTTAAGGGCCAGCAGCACAAAGGGATTCGTCCTCGGCATCTCCGGCGGGCAGGACAGTG
CGCTGGCCGGGCGACTGTGCCAGCTGGCGGCACAGGAATCGCGGCTCGAAGGAGTGGCCGCCGAGTTCATCGCGGTGCGG
CTGCCATACGGCGTGCAGGCCGATGAAGAAGATGCCCAAGTGGCCCTTCGCTTTATCGACCCCGACCGCACCATCGTCAT
CAACATCAAGGAAACCTCCGACGCTGCGACCAAGGCGGTCGCCGAGGCACTCGGCGAGACCCCGACGGACTTCGTGCGCG
GCAACATCAAAGCCCGGGAGCGGATGGTCGTCCAGTACGCGGCTGCCGGCCAACACCGGCTACTGGTCGTCGGCACCGAC
CACGCGGCCGAGGCTGTCACAGGGTTCTTCACCAAGTTCGGCGACGGCGGAGTCGATGTCACCCCACTTACTGGACTCAC
CAAACGCCAAGGCGCGCAAATCCTTTCTCACCTCGGCGCACCGGACAGCATTTCCCATAAGGTGCCCACAGCCGATCTGG
AGGACGATCGGCCCGCTCTTCCCGACGAGGTCGCCCTGGGCGTCACCTACGCGCAGATCGACGACTATCTGGAGGGCAAG
GCCGTCACCGTCGAGGCCGCCGACCGCATTGAGCGGTGGTACCTACAGACCCGTCACAAGCGGGCGCAGCCGGTCACGCC
ATTTGACGGTTGGTGGCGTTAG

Upstream 100 bases:

>100_bases
ACCAACTCCAACGGCCAGTCGGTCGCCCAACTCGGCGACGCGTGGGAGGACCTGGTGCACAAGCTGTGCGCACGCTGACC
GAGCGTTCTACAGTCGGGCC

Downstream 100 bases:

>100_bases
GAGTCCCGCGGCGACGACGCCGAACCTGTCTTCTGGAATCCGGGCCGCAGCGCGTTGGTAAACGCGTAGTTGGCCACCCG
CATCGCGACGCTGTACGGCC

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MSSLREEIRSALDVSPTIDPAAEVSRRVGFLKDYLRASSTKGFVLGISGGQDSALAGRLCQLAAQESRLEGVAAEFIAVR
LPYGVQADEEDAQVALRFIDPDRTIVINIKETSDAATKAVAEALGETPTDFVRGNIKARERMVVQYAAAGQHRLLVVGTD
HAAEAVTGFFTKFGDGGVDVTPLTGLTKRQGAQILSHLGAPDSISHKVPTADLEDDRPALPDEVALGVTYAQIDDYLEGK
AVTVEAADRIERWYLQTRHKRAQPVTPFDGWWR

Sequences:

>Translated_273_residues
MSSLREEIRSALDVSPTIDPAAEVSRRVGFLKDYLRASSTKGFVLGISGGQDSALAGRLCQLAAQESRLEGVAAEFIAVR
LPYGVQADEEDAQVALRFIDPDRTIVINIKETSDAATKAVAEALGETPTDFVRGNIKARERMVVQYAAAGQHRLLVVGTD
HAAEAVTGFFTKFGDGGVDVTPLTGLTKRQGAQILSHLGAPDSISHKVPTADLEDDRPALPDEVALGVTYAQIDDYLEGK
AVTVEAADRIERWYLQTRHKRAQPVTPFDGWWR
>Mature_272_residues
SSLREEIRSALDVSPTIDPAAEVSRRVGFLKDYLRASSTKGFVLGISGGQDSALAGRLCQLAAQESRLEGVAAEFIAVRL
PYGVQADEEDAQVALRFIDPDRTIVINIKETSDAATKAVAEALGETPTDFVRGNIKARERMVVQYAAAGQHRLLVVGTDH
AAEAVTGFFTKFGDGGVDVTPLTGLTKRQGAQILSHLGAPDSISHKVPTADLEDDRPALPDEVALGVTYAQIDDYLEGKA
VTVEAADRIERWYLQTRHKRAQPVTPFDGWWR

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family

Homologues:

Organism=Escherichia coli, GI1788036, Length=274, Percent_Identity=58.3941605839416, Blast_Score=315, Evalue=2e-87,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NADE_MYCA9 (B1ME26)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001704149.1
- ProteinModelPortal:   B1ME26
- SMR:   B1ME26
- EnsemblBacteria:   EBMYCT00000006381
- GeneID:   5965919
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_3419
- HOGENOM:   HBG351567
- OMA:   GLNKRRV
- ProtClustDB:   PRK00768
- BioCyc:   MABS561007:MAB_3419-MONOMER
- HAMAP:   MF_00193
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00552

Pfam domain/function: PF02540 NAD_synthase

EC number: =6.3.1.5

Molecular weight: Translated: 29587; Mature: 29455

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: ACT_SITE 48-48

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLREEIRSALDVSPTIDPAAEVSRRVGFLKDYLRASSTKGFVLGISGGQDSALAGRLC
CCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHH
QLAAQESRLEGVAAEFIAVRLPYGVQADEEDAQVALRFIDPDRTIVINIKETSDAATKAV
HHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCHHHHHHH
AEALGETPTDFVRGNIKARERMVVQYAAAGQHRLLVVGTDHAAEAVTGFFTKFGDGGVDV
HHHHCCCCHHHHHCCHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCEE
TPLTGLTKRQGAQILSHLGAPDSISHKVPTADLEDDRPALPDEVALGVTYAQIDDYLEGK
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHCCC
AVTVEAADRIERWYLQTRHKRAQPVTPFDGWWR
EEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SSLREEIRSALDVSPTIDPAAEVSRRVGFLKDYLRASSTKGFVLGISGGQDSALAGRLC
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHH
QLAAQESRLEGVAAEFIAVRLPYGVQADEEDAQVALRFIDPDRTIVINIKETSDAATKAV
HHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCHHHHHHH
AEALGETPTDFVRGNIKARERMVVQYAAAGQHRLLVVGTDHAAEAVTGFFTKFGDGGVDV
HHHHCCCCHHHHHCCHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCEE
TPLTGLTKRQGAQILSHLGAPDSISHKVPTADLEDDRPALPDEVALGVTYAQIDDYLEGK
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHCCC
AVTVEAADRIERWYLQTRHKRAQPVTPFDGWWR
EEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA