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The map label for this gene is nfnB [H]

Identifier: 169630391

GI number: 169630391

Start: 3349037

End: 3349684

Strand: Reverse

Name: nfnB [H]

Synonym: MAB_3310c

Alternate gene names: 169630391

Gene position: 3349684-3349037 (Counterclockwise)

Preceding gene: 169630392

Following gene: 169630390

Centisome position: 66.11

GC content: 59.26

Gene sequence:

>648_bases
ATGAACCTCGACAACCTCATGAGTAAGCACCTCACGAGATATTTCGACTCCAGCAAGACCATTCCGGCCGAGTCCCTGCA
GCAATTGTTGCGGTTCCTGAGGACGGTTCCGTCATCGGTGAACGTGCAGGCTAGTCGTTACTACGTGCTCGGCACGCCCG
AAGGCAAGGAGCGGCTCGCAGGCAATCTGGGCGAACGGTTCCTCGACAACGGCGGCAAGATCCGGGACGCCTCGCATGTC
CTGGTCTTCACCACCAGGGCCGACGTGCCCGATAGCCACCTCGAAGAGGTGTTCGGCAAAGAGAAAGCCGATGGCAGGTT
CCCGGACCCCGCGAAGCAGTCGCGGTGGGAATCAATGACCCGCGACTTCCTGAACCTGAGGACCTATGGCTACAAGGACC
TAACCCACTGGATGGAGAAGCAGACCTATCTGGCGTTGGGCATGACCATGATGGCCGCCGCCGAGCTGGGTTTCGATGCC
ACGCCGCTGGAAGGTTTCGACCCGGCCACTGTCGATGAGGCATTCAAAATCCGAGAAACCGGATACACCACAACGGTACT
GCTGGCACTCGGATACCCGGACGCCGCGAACACATACAACAACCCGATTTCACGGTTGGACCACGACAGGCTCTTCACCT
TCGCCTGA

Upstream 100 bases:

>100_bases
GACCAACTACATCAACAATGTCAACCAAACCGTCATCGACATCCCGGCGGCCGGCACCGCCGCGTGACGCTCAAACACCT
TGCAAGGAAAGTAAATTGAC

Downstream 100 bases:

>100_bases
ACCGCATCACGCAACCCAATTCATCTGAACGTTCTCATACGAAAGGTATCGAAAATGTCCACCAGAACCGACGCGCTGAG
CGTCGTATGCCAAAACAATG

Product: oxygen-insensitive NAD(P)H nitroreductase

Products: NA

Alternate protein names: NR [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MNLDNLMSKHLTRYFDSSKTIPAESLQQLLRFLRTVPSSVNVQASRYYVLGTPEGKERLAGNLGERFLDNGGKIRDASHV
LVFTTRADVPDSHLEEVFGKEKADGRFPDPAKQSRWESMTRDFLNLRTYGYKDLTHWMEKQTYLALGMTMMAAAELGFDA
TPLEGFDPATVDEAFKIRETGYTTTVLLALGYPDAANTYNNPISRLDHDRLFTFA

Sequences:

>Translated_215_residues
MNLDNLMSKHLTRYFDSSKTIPAESLQQLLRFLRTVPSSVNVQASRYYVLGTPEGKERLAGNLGERFLDNGGKIRDASHV
LVFTTRADVPDSHLEEVFGKEKADGRFPDPAKQSRWESMTRDFLNLRTYGYKDLTHWMEKQTYLALGMTMMAAAELGFDA
TPLEGFDPATVDEAFKIRETGYTTTVLLALGYPDAANTYNNPISRLDHDRLFTFA
>Mature_215_residues
MNLDNLMSKHLTRYFDSSKTIPAESLQQLLRFLRTVPSSVNVQASRYYVLGTPEGKERLAGNLGERFLDNGGKIRDASHV
LVFTTRADVPDSHLEEVFGKEKADGRFPDPAKQSRWESMTRDFLNLRTYGYKDLTHWMEKQTYLALGMTMMAAAELGFDA
TPLEGFDPATVDEAFKIRETGYTTTVLLALGYPDAANTYNNPISRLDHDRLFTFA

Specific function: Reduction of a variety of nitroaromatic compounds using NADH (and to lesser extent NADPH) as source of reducing equivalents; two electrons are transferred [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

Organism=Escherichia coli, GI1786792, Length=186, Percent_Identity=36.5591397849462, Blast_Score=133, Evalue=7e-33,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.-; 1.5.1.34

Molecular weight: Translated: 24330; Mature: 24330

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLDNLMSKHLTRYFDSSKTIPAESLQQLLRFLRTVPSSVNVQASRYYVLGTPEGKERLA
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEECEEEEEECCCHHHHHH
GNLGERFLDNGGKIRDASHVLVFTTRADVPDSHLEEVFGKEKADGRFPDPAKQSRWESMT
HHHHHHHHHCCCCEECCCEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
RDFLNLRTYGYKDLTHWMEKQTYLALGMTMMAAAELGFDATPLEGFDPATVDEAFKIRET
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHC
GYTTTVLLALGYPDAANTYNNPISRLDHDRLFTFA
CCCEEEEEEECCCCCCCCCCCHHHHCCCCHHEECC
>Mature Secondary Structure
MNLDNLMSKHLTRYFDSSKTIPAESLQQLLRFLRTVPSSVNVQASRYYVLGTPEGKERLA
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEECEEEEEECCCHHHHHH
GNLGERFLDNGGKIRDASHVLVFTTRADVPDSHLEEVFGKEKADGRFPDPAKQSRWESMT
HHHHHHHHHCCCCEECCCEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
RDFLNLRTYGYKDLTHWMEKQTYLALGMTMMAAAELGFDATPLEGFDPATVDEAFKIRET
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHC
GYTTTVLLALGYPDAANTYNNPISRLDHDRLFTFA
CCCEEEEEEECCCCCCCCCCCHHHHCCCCHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1999406; 1999405 [H]