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The map label for this gene is yogA [H]

Identifier: 169630388

GI number: 169630388

Start: 3346304

End: 3347221

Strand: Reverse

Name: yogA [H]

Synonym: MAB_3307c

Alternate gene names: 169630388

Gene position: 3347221-3346304 (Counterclockwise)

Preceding gene: 169630390

Following gene: 169630387

Centisome position: 66.06

GC content: 62.96

Gene sequence:

>918_bases
ATGAAAGCGATCGTTGCCGAACAGGGCGCGAAGGGGGCCGGACTGTCGTTGACGGCAGTGCCCTATCCCCATGCCGCAGA
GAACGACGTCATCGTCCAGGTGCACGCCGCCGGATTCACTCCCGGGGAGCTCGATTGGCCAGGAACCTGGGCCGACCGTT
CGGGCCGGAGCCGAGGCCCGAGCATCCCAGGGCACGAGGTATCGGGCATCGTGGTCGAACTAGGCTACGGCACAACGGGG
TTGACTGTGGGGCAGCGAGTCTTTGGCATCACGGACTGGACGCGCAATGGCGCGTTGGCGCAGTACGTCGCGGTGGAAGC
GCGGAATCTGGCTCCTCTGTCGGCCGATGTGGACCACACGGTTGCGGCCGCCCTACCCGTTTCCGGTCTGACGGCCTGGC
AAGGGCTATTCCTCCACGGACACATCGTCGCGGGGCAGAGCGTTCTCATACATGGTGCGGCAGGTGCGGTCGGTTCTGTG
GCCGTGCAGCTTGCACACGAGGCGGGAGCTCACGTCATCGGCACCGGACGCGGCGGCCAACGTGATCAGGTCTTGGGCCT
AAAAGCCGACGATTTCGTCGACCTGACCACTGACACACTCGAAGACATCGGCCAGGTCGACGTTGTATTCGATGTGATTG
GTGGGGAGATCCTCGATCGCTCAGCGCAACTGGTGCGTGCCGGAGGCGCGTTGATCACCATCGCCGAACCCCCGCGTGTC
ATCCCCGAGGGCGCACGGGCCGTTTTCTTTGTCGTTGAGGCGGACCGCAGACAGCTCACTATCTTGGAGAACAAACTGCG
CGACGGGCGAATCCGTCAGAACATCGGATCGGTAATCCCCCTTGAGGATGCGTTGAGCGCCTTCGATATCGATCGAAAGA
CTCCCGGCAAGACGATCGTCCAAATAGCGGCTGTCTAG

Upstream 100 bases:

>100_bases
ATCAAAAAGATCTGTTGGACTAGATGACTCCCGGCGGCTGTGATGGAAGGACAACACGGACGAAAGGACGAATATGCCGG
ACCCGGGTAACGCGAAAGTC

Downstream 100 bases:

>100_bases
GGTCGCGGTGCTACCTGGTCGTACTCAGTTCACCATGTCGATTCGAAAGAGGTTGAGGCCAAATGACTCTGAAGATTCTA
CCCGTAACTCTGGGCGTTGC

Product: putative zinc-binding oxidoreductase

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MKAIVAEQGAKGAGLSLTAVPYPHAAENDVIVQVHAAGFTPGELDWPGTWADRSGRSRGPSIPGHEVSGIVVELGYGTTG
LTVGQRVFGITDWTRNGALAQYVAVEARNLAPLSADVDHTVAAALPVSGLTAWQGLFLHGHIVAGQSVLIHGAAGAVGSV
AVQLAHEAGAHVIGTGRGGQRDQVLGLKADDFVDLTTDTLEDIGQVDVVFDVIGGEILDRSAQLVRAGGALITIAEPPRV
IPEGARAVFFVVEADRRQLTILENKLRDGRIRQNIGSVIPLEDALSAFDIDRKTPGKTIVQIAAV

Sequences:

>Translated_305_residues
MKAIVAEQGAKGAGLSLTAVPYPHAAENDVIVQVHAAGFTPGELDWPGTWADRSGRSRGPSIPGHEVSGIVVELGYGTTG
LTVGQRVFGITDWTRNGALAQYVAVEARNLAPLSADVDHTVAAALPVSGLTAWQGLFLHGHIVAGQSVLIHGAAGAVGSV
AVQLAHEAGAHVIGTGRGGQRDQVLGLKADDFVDLTTDTLEDIGQVDVVFDVIGGEILDRSAQLVRAGGALITIAEPPRV
IPEGARAVFFVVEADRRQLTILENKLRDGRIRQNIGSVIPLEDALSAFDIDRKTPGKTIVQIAAV
>Mature_305_residues
MKAIVAEQGAKGAGLSLTAVPYPHAAENDVIVQVHAAGFTPGELDWPGTWADRSGRSRGPSIPGHEVSGIVVELGYGTTG
LTVGQRVFGITDWTRNGALAQYVAVEARNLAPLSADVDHTVAAALPVSGLTAWQGLFLHGHIVAGQSVLIHGAAGAVGSV
AVQLAHEAGAHVIGTGRGGQRDQVLGLKADDFVDLTTDTLEDIGQVDVVFDVIGGEILDRSAQLVRAGGALITIAEPPRV
IPEGARAVFFVVEADRRQLTILENKLRDGRIRQNIGSVIPLEDALSAFDIDRKTPGKTIVQIAAV

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI47519420, Length=268, Percent_Identity=27.6119402985075, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI194239674, Length=295, Percent_Identity=26.7796610169492, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI13236495, Length=295, Percent_Identity=26.7796610169492, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI194239676, Length=160, Percent_Identity=33.125, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI22538446, Length=225, Percent_Identity=30.6666666666667, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI22538444, Length=225, Percent_Identity=30.6666666666667, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI24308257, Length=229, Percent_Identity=26.2008733624454, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI28557745, Length=223, Percent_Identity=29.1479820627803, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI18379349, Length=181, Percent_Identity=30.939226519337, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1790485, Length=235, Percent_Identity=30.2127659574468, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71987554, Length=227, Percent_Identity=27.7533039647577, Blast_Score=71, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6319621, Length=212, Percent_Identity=27.3584905660377, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324486, Length=283, Percent_Identity=23.321554770318, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 31776; Mature: 31776

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAIVAEQGAKGAGLSLTAVPYPHAAENDVIVQVHAAGFTPGELDWPGTWADRSGRSRGP
CCCEECCCCCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
SIPGHEVSGIVVELGYGTTGLTVGQRVFGITDWTRNGALAQYVAVEARNLAPLSADVDHT
CCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCCHH
VAAALPVSGLTAWQGLFLHGHIVAGQSVLIHGAAGAVGSVAVQLAHEAGAHVIGTGRGGQ
HHHHCCCCCCHHHCEEEEEEEEEECCEEEEEECCCCHHHHHHHHHHHCCCEEEECCCCCC
RDQVLGLKADDFVDLTTDTLEDIGQVDVVFDVIGGEILDRSAQLVRAGGALITIAEPPRV
CCEEEECCCCCEEECCHHHHHHCCCCEEEEEHHCCHHHHHHHHHHHCCCCEEEECCCCCC
IPEGARAVFFVVEADRRQLTILENKLRDGRIRQNIGSVIPLEDALSAFDIDRKTPGKTIV
CCCCCEEEEEEEECCCCEEEEEHHHCCCCHHHHHCCCCCCHHHHHHHHCCCCCCCCCEEE
QIAAV
EEEEC
>Mature Secondary Structure
MKAIVAEQGAKGAGLSLTAVPYPHAAENDVIVQVHAAGFTPGELDWPGTWADRSGRSRGP
CCCEECCCCCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
SIPGHEVSGIVVELGYGTTGLTVGQRVFGITDWTRNGALAQYVAVEARNLAPLSADVDHT
CCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCCHH
VAAALPVSGLTAWQGLFLHGHIVAGQSVLIHGAAGAVGSVAVQLAHEAGAHVIGTGRGGQ
HHHHCCCCCCHHHCEEEEEEEEEECCEEEEEECCCCHHHHHHHHHHHCCCEEEECCCCCC
RDQVLGLKADDFVDLTTDTLEDIGQVDVVFDVIGGEILDRSAQLVRAGGALITIAEPPRV
CCEEEECCCCCEEECCHHHHHHCCCCEEEEEHHCCHHHHHHHHHHHCCCCEEEECCCCCC
IPEGARAVFFVVEADRRQLTILENKLRDGRIRQNIGSVIPLEDALSAFDIDRKTPGKTIV
CCCCCEEEEEEEECCCCEEEEEHHHCCCCHHHHHCCCCCCHHHHHHHHCCCCCCCCCEEE
QIAAV
EEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]