Click here to switch to the map view.

The map label for this gene is lip2 [H]

Identifier: 169630351

GI number: 169630351

Start: 3308573

End: 3309619

Strand: Reverse

Name: lip2 [H]

Synonym: MAB_3270c

Alternate gene names: 169630351

Gene position: 3309619-3308573 (Counterclockwise)

Preceding gene: 169630353

Following gene: 169630350

Centisome position: 65.31

GC content: 67.53

Gene sequence:

>1047_bases
GTGAGCCCTTTCCGCGACTTCGCCCTGCGTGCCAGCACCGCCGCGACACCGCATATCCCGACTCCGGTTCGACGGGCCAT
GGCCGGTCGCCAGGTGATCATCGACGGCAACATCCTGGACCCGACCACGCAGCTATTGCTGCGCGGTATGGAGTTGATGG
GGGAGGGGGAGATATCGCGCGGAGAGGATGTTGCCGAGAACCGGGCCAATATGCGCAGGCAGGCGGGGTTGCTGCAGTTC
AGCACGCCGGTGGGGCAGGTGCGCAACTTCTCCATCCCCGGGCCGGCCGGCCCCATCGGGGTCCGGCACTACAAGCCGGT
ACATGATCACACCGGGGCCCTGCTGGTGTTCTTCCACGGCGGCGGCTGGGTGATCGGTGACCTGGAGACCCATGACCAGC
CGTGCCGCCAGACCTGTCGGGATGCCGGTGTCTCGGTGTTGTCCGTGGACTATCGGCTGGCTCCCGAGCACAAGGCACCC
GCCGCCGCGCAGGACTGCCTGGCCGCGTACCTGTGGGCCGTCGAGCATGCCGGAGATCTGGGCGTGGCACCGGACAGGAT
CGCGGTGGGCGGTGACAGCGCGGGCGGCAATCTGGCGGCAGTCGTCGCGCAGCAGGCACGTGACACCGGCGCGCAGCTGC
CGCTGCTGCAGTTCCTGATCTACCCGGCCGTCGACTTCACGGTGCGCAGGCCGTCACGGGATCTATTCGCCGAGGGGTTC
TTTCTCACCAAGGCGGCCATGGACGGATTCGAAAGTCACTATCTTGACGGGTCCGACGTCGATAAGGCGGACCCCCAAGT
GTCACCGATTTTGGCCGCGGACCTGGCGGGGTTGCCGCCGGCGCTTATCACGACGGCGGGCTTTGATCCGCTGCGCGACG
AGGGCAACGAATACGCCGCGAAGCTGCGGGCCGCCGGAGTGCCGGTGGACCTGCGGGTACAGGGCCCGCTGATTCATGGC
TTTTACAACATGGCCGGGCTGGGTGGCGCGCCGTCGGAGGCCATGAATCAGCTGACCTCGGCGCTGCGCGCGCATCTGTC
CCGCTAA

Upstream 100 bases:

>100_bases
CAAGTGATCGTCGACTCGCGGGTGTAGTCGCGGCACATGAAGGGCAGCGCAATGCCGATATCCATACCGGCAGCCTAGGA
GTTCTCGCTACGGTTGTGGC

Downstream 100 bases:

>100_bases
TCCTGCCCGGTAGGCTGTTCGCGTGGCCAAACCGAAGCAACCCCCCAGCTACGACCTCAAGAAGGCGGAGCAGCGCCGAA
GCCAGTTGATTCAAATCGGG

Product: lipase/esterase LipN

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MSPFRDFALRASTAATPHIPTPVRRAMAGRQVIIDGNILDPTTQLLLRGMELMGEGEISRGEDVAENRANMRRQAGLLQF
STPVGQVRNFSIPGPAGPIGVRHYKPVHDHTGALLVFFHGGGWVIGDLETHDQPCRQTCRDAGVSVLSVDYRLAPEHKAP
AAAQDCLAAYLWAVEHAGDLGVAPDRIAVGGDSAGGNLAAVVAQQARDTGAQLPLLQFLIYPAVDFTVRRPSRDLFAEGF
FLTKAAMDGFESHYLDGSDVDKADPQVSPILAADLAGLPPALITTAGFDPLRDEGNEYAAKLRAAGVPVDLRVQGPLIHG
FYNMAGLGGAPSEAMNQLTSALRAHLSR

Sequences:

>Translated_348_residues
MSPFRDFALRASTAATPHIPTPVRRAMAGRQVIIDGNILDPTTQLLLRGMELMGEGEISRGEDVAENRANMRRQAGLLQF
STPVGQVRNFSIPGPAGPIGVRHYKPVHDHTGALLVFFHGGGWVIGDLETHDQPCRQTCRDAGVSVLSVDYRLAPEHKAP
AAAQDCLAAYLWAVEHAGDLGVAPDRIAVGGDSAGGNLAAVVAQQARDTGAQLPLLQFLIYPAVDFTVRRPSRDLFAEGF
FLTKAAMDGFESHYLDGSDVDKADPQVSPILAADLAGLPPALITTAGFDPLRDEGNEYAAKLRAAGVPVDLRVQGPLIHG
FYNMAGLGGAPSEAMNQLTSALRAHLSR
>Mature_347_residues
SPFRDFALRASTAATPHIPTPVRRAMAGRQVIIDGNILDPTTQLLLRGMELMGEGEISRGEDVAENRANMRRQAGLLQFS
TPVGQVRNFSIPGPAGPIGVRHYKPVHDHTGALLVFFHGGGWVIGDLETHDQPCRQTCRDAGVSVLSVDYRLAPEHKAPA
AAQDCLAAYLWAVEHAGDLGVAPDRIAVGGDSAGGNLAAVVAQQARDTGAQLPLLQFLIYPAVDFTVRRPSRDLFAEGFF
LTKAAMDGFESHYLDGSDVDKADPQVSPILAADLAGLPPALITTAGFDPLRDEGNEYAAKLRAAGVPVDLRVQGPLIHGF
YNMAGLGGAPSEAMNQLTSALRAHLSR

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=294, Percent_Identity=30.2721088435374, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI21328446, Length=112, Percent_Identity=40.1785714285714, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI157041239, Length=109, Percent_Identity=44.0366972477064, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI68051721, Length=262, Percent_Identity=28.2442748091603, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI206597554, Length=260, Percent_Identity=30, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI61966717, Length=255, Percent_Identity=27.0588235294118, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI157041237, Length=96, Percent_Identity=42.7083333333333, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI226423947, Length=270, Percent_Identity=27.4074074074074, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1786682, Length=278, Percent_Identity=28.4172661870504, Blast_Score=104, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17567059, Length=274, Percent_Identity=31.7518248175182, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI72001146, Length=132, Percent_Identity=36.3636363636364, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71996133, Length=131, Percent_Identity=35.8778625954198, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17540028, Length=132, Percent_Identity=37.1212121212121, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI115533410, Length=96, Percent_Identity=35.4166666666667, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI115533412, Length=96, Percent_Identity=35.4166666666667, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24656084, Length=165, Percent_Identity=32.7272727272727, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24656076, Length=165, Percent_Identity=32.7272727272727, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI20130169, Length=165, Percent_Identity=32.7272727272727, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 37037; Mature: 36906

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPFRDFALRASTAATPHIPTPVRRAMAGRQVIIDGNILDPTTQLLLRGMELMGEGEISR
CCCHHHHHHHHCCCCCCCCCHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCC
GEDVAENRANMRRQAGLLQFSTPVGQVRNFSIPGPAGPIGVRHYKPVHDHTGALLVFFHG
CHHHHHHHHHHHHHHCCEEECCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEC
GGWVIGDLETHDQPCRQTCRDAGVSVLSVDYRLAPEHKAPAAAQDCLAAYLWAVEHAGDL
CCEEEECCCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCC
GVAPDRIAVGGDSAGGNLAAVVAQQARDTGAQLPLLQFLIYPAVDFTVRRPSRDLFAEGF
CCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCCHHHHHCCH
FLTKAAMDGFESHYLDGSDVDKADPQVSPILAADLAGLPPALITTAGFDPLRDEGNEYAA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHCCCCHHHHCCHHHHH
KLRAAGVPVDLRVQGPLIHGFYNMAGLGGAPSEAMNQLTSALRAHLSR
HHHHCCCCEEEEECCCEEEHHHHHCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SPFRDFALRASTAATPHIPTPVRRAMAGRQVIIDGNILDPTTQLLLRGMELMGEGEISR
CCHHHHHHHHCCCCCCCCCHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCC
GEDVAENRANMRRQAGLLQFSTPVGQVRNFSIPGPAGPIGVRHYKPVHDHTGALLVFFHG
CHHHHHHHHHHHHHHCCEEECCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEC
GGWVIGDLETHDQPCRQTCRDAGVSVLSVDYRLAPEHKAPAAAQDCLAAYLWAVEHAGDL
CCEEEECCCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCC
GVAPDRIAVGGDSAGGNLAAVVAQQARDTGAQLPLLQFLIYPAVDFTVRRPSRDLFAEGF
CCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCCHHHHHCCH
FLTKAAMDGFESHYLDGSDVDKADPQVSPILAADLAGLPPALITTAGFDPLRDEGNEYAA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHCCCCHHHHCCHHHHH
KLRAAGVPVDLRVQGPLIHGFYNMAGLGGAPSEAMNQLTSALRAHLSR
HHHHCCCCEEEEECCCEEEHHHHHCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]