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The map label for this gene is rsmD [H]

Identifier: 169630347

GI number: 169630347

Start: 3303224

End: 3303667

Strand: Reverse

Name: rsmD [H]

Synonym: MAB_3266c

Alternate gene names: 169630347

Gene position: 3303667-3303224 (Counterclockwise)

Preceding gene: 169630348

Following gene: 169630346

Centisome position: 65.2

GC content: 64.86

Gene sequence:

>444_bases
ATGCTGGAGGCGCGCCTGGACTTTGAGGGGATCGCGGTGCTCGATCTCTTCGCCGGTTCTGGCGCGTTGGGGCTGGAGGC
GCTCTCGCGCGGAGCCCAACACGTGACATTCGTCGAGTCCAATGCCGCCGCGTCCAGGGTGATATCGGCCAACATTGCGA
CGGTGGGGCTGCCCGGTGCCGTGCTGCGGCAATGCCCGGTATCGACCTTTCTTTCGATGTCACCCGACCGCGCCTATGAC
CTTGTTCTTGCCGATCCGCCGTACTCGGTTTCGGCCGAGGAGGTTTCCGCGCTGTTATCGCGGTTGACGGATCATTGGGT
CACCGACGAGGCCCTGGTAGTGCTGGAGCGCGATGCGTCAGGACCCGAGGCGGTGTGGCCGGACGGCTGGGAACTGATGT
CCCGCAAGTACGGCGGCACCCGGCTAGAGCTGGGATCCCTGTAA

Upstream 100 bases:

>100_bases
GACCCGGATTATCGCCGGTGTCGCGGGCGGGCGCCGGATCGCGGTGCCGCCAAAGGGAACCCGGCCCACCACGGATCGGG
TGCGAGAAGCGTTGTTCAGC

Downstream 100 bases:

>100_bases
GCGTCCAGGCGATCCACAGCCACCGTTTACTTGTCGAGCGCAGACTGCTCGTCATGACCCCGCTGCAGCGATATATCGCC
GAAGAGATCGCCACGGACCA

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 147; Mature: 147

Protein sequence:

>147_residues
MLEARLDFEGIAVLDLFAGSGALGLEALSRGAQHVTFVESNAAASRVISANIATVGLPGAVLRQCPVSTFLSMSPDRAYD
LVLADPPYSVSAEEVSALLSRLTDHWVTDEALVVLERDASGPEAVWPDGWELMSRKYGGTRLELGSL

Sequences:

>Translated_147_residues
MLEARLDFEGIAVLDLFAGSGALGLEALSRGAQHVTFVESNAAASRVISANIATVGLPGAVLRQCPVSTFLSMSPDRAYD
LVLADPPYSVSAEEVSALLSRLTDHWVTDEALVVLERDASGPEAVWPDGWELMSRKYGGTRLELGSL
>Mature_147_residues
MLEARLDFEGIAVLDLFAGSGALGLEALSRGAQHVTFVESNAAASRVISANIATVGLPGAVLRQCPVSTFLSMSPDRAYD
LVLADPPYSVSAEEVSALLSRLTDHWVTDEALVVLERDASGPEAVWPDGWELMSRKYGGTRLELGSL

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=128, Percent_Identity=35.9375, Blast_Score=68, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 15632; Mature: 15632

Theoretical pI: Translated: 4.21; Mature: 4.21

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEARLDFEGIAVLDLFAGSGALGLEALSRGAQHVTFVESNAAASRVISANIATVGLPGA
CCCCCCCCCCEEEEEEECCCCCCHHHHHHCCCCEEEEEECCCHHHHHEECCCEEECCCHH
VLRQCPVSTFLSMSPDRAYDLVLADPPYSVSAEEVSALLSRLTDHWVTDEALVVLERDAS
HHHHCCHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCC
GPEAVWPDGWELMSRKYGGTRLELGSL
CCCCCCCCHHHHHHHCCCCCEEEECCC
>Mature Secondary Structure
MLEARLDFEGIAVLDLFAGSGALGLEALSRGAQHVTFVESNAAASRVISANIATVGLPGA
CCCCCCCCCCEEEEEEECCCCCCHHHHHHCCCCEEEEEECCCHHHHHEECCCEEECCCHH
VLRQCPVSTFLSMSPDRAYDLVLADPPYSVSAEEVSALLSRLTDHWVTDEALVVLERDAS
HHHHCCHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCC
GPEAVWPDGWELMSRKYGGTRLELGSL
CCCCCCCCHHHHHHHCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]