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The map label for this gene is ispG [H]

Identifier: 169630251

GI number: 169630251

Start: 3216163

End: 3217350

Strand: Reverse

Name: ispG [H]

Synonym: MAB_3169c

Alternate gene names: 169630251

Gene position: 3217350-3216163 (Counterclockwise)

Preceding gene: 169630252

Following gene: 169630250

Centisome position: 63.49

GC content: 64.56

Gene sequence:

>1188_bases
ATGATGACCGGTCTTGGCATGCCCAGTCTTCCTGGCGCACCGCCGCCGACATTGGCGCCGCGGCGTCAGACGCGTCAGCT
CATGGTGGGAAGCGTCGGGGTGGGCAGCGACTCACCGGTCTCGGTGCAATCGATGTGCACCACCAAGACCCATGACGTGA
ATTCCACCCTGCAGCAGATCGCGGAGCTCACAGCCTCTGGCTGCGACATCGTGCGTGTCGCCTGCCCGCGGCAGGAGGAC
GCCGATGCGCTTGCCGAGATTGCCCGCAAGAGCAAGATTCCGGTGATCGCCGATATCCATTTTCAGCCCAAGTACATCTT
CGCGGCGATCGATGCCGGCTGTGCCGCGGTGCGCGTGAACCCGGGAAACATCAAGGAGTTCGACGGACGCGTCAAGGAGG
TTGCCAAGGCGGCCGGTGACGCCGGGATCCCCATCCGGATCGGCGTCAACGCCGGGTCCCTTGATCCGCGGATCCTCGGC
AAGTACGGAAAGGCAACCCCGGAGGCGCTCGTCGAGTCGGCGCTGTGGGAGGCCGGGCTGTTCGAGGAGCACGGCTTCGG
AGACATCAAGATCAGCGTCAAGCACAACGACCCGGTGATCATGGTCGCCGCCTACGAACTGCTGGCCGCACAGTGCGATT
ACCCGCTGCACCTGGGTGTGACGGAGGCCGGTCCGGCATTTCAGGGCACCATCAAGTCGGCGGTCGCCTTTGGAGCGCTG
TTGTCCAAGGGCATCGGTGACACCATCCGGGTTTCCTTGTCGGCGCCGCCCGCCGAAGAGGTCAAGGTCGGCAATCAGAT
CCTCGAATCGCTCAATCTGCGTCCCCGCTCACTGGAAATCGTGTCCTGCCCGTCGTGCGGGCGCGCACAGGTGGACGTCT
ACACGCTGGCCAACGCCGTCAGCGCGGGGCTAGAGGGCCTGGATGTGCCGCTGCGTGTCGCGGTCATGGGTTGCGTCGTC
AATGGGCCAGGAGAGGCGCGTGAGGCCGACCTGGGCGTGGCGTCCGGTAATGGCAAGGGACAGATCTTCGTGAAGGGTGA
GGTCATCAAGACCGTGCCCGAGGCGCTGATCGTCGAGACGCTCATTGATGAGGCCATGCGATTGGCCGAGCAGATGGGTC
TGGAAAACGGCGCAGGGATCGGAAGCGACGGAACCCCTACAGGCCAGCCGGTCGTCACCGTAAGCTGA

Upstream 100 bases:

>100_bases
GCCGGCGACCTACCTCGTTCTTGTGGTGGTGGTCGGGTACATGCTGCTCACCATCACCGCCGACATCGTCAACCCGATTA
GACTTTTCCAATAGGAGATA

Downstream 100 bases:

>100_bases
ATGTAGCTCGGCGCGAGTGGATGAACCGATGAGCGCTCGCGCGAAGAGGCTTGAGCACGGGAGCGATTGCCGATGACGAC
AACACTGCCGGCGCCTGGAG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MMTGLGMPSLPGAPPPTLAPRRQTRQLMVGSVGVGSDSPVSVQSMCTTKTHDVNSTLQQIAELTASGCDIVRVACPRQED
ADALAEIARKSKIPVIADIHFQPKYIFAAIDAGCAAVRVNPGNIKEFDGRVKEVAKAAGDAGIPIRIGVNAGSLDPRILG
KYGKATPEALVESALWEAGLFEEHGFGDIKISVKHNDPVIMVAAYELLAAQCDYPLHLGVTEAGPAFQGTIKSAVAFGAL
LSKGIGDTIRVSLSAPPAEEVKVGNQILESLNLRPRSLEIVSCPSCGRAQVDVYTLANAVSAGLEGLDVPLRVAVMGCVV
NGPGEAREADLGVASGNGKGQIFVKGEVIKTVPEALIVETLIDEAMRLAEQMGLENGAGIGSDGTPTGQPVVTVS

Sequences:

>Translated_395_residues
MMTGLGMPSLPGAPPPTLAPRRQTRQLMVGSVGVGSDSPVSVQSMCTTKTHDVNSTLQQIAELTASGCDIVRVACPRQED
ADALAEIARKSKIPVIADIHFQPKYIFAAIDAGCAAVRVNPGNIKEFDGRVKEVAKAAGDAGIPIRIGVNAGSLDPRILG
KYGKATPEALVESALWEAGLFEEHGFGDIKISVKHNDPVIMVAAYELLAAQCDYPLHLGVTEAGPAFQGTIKSAVAFGAL
LSKGIGDTIRVSLSAPPAEEVKVGNQILESLNLRPRSLEIVSCPSCGRAQVDVYTLANAVSAGLEGLDVPLRVAVMGCVV
NGPGEAREADLGVASGNGKGQIFVKGEVIKTVPEALIVETLIDEAMRLAEQMGLENGAGIGSDGTPTGQPVVTVS
>Mature_395_residues
MMTGLGMPSLPGAPPPTLAPRRQTRQLMVGSVGVGSDSPVSVQSMCTTKTHDVNSTLQQIAELTASGCDIVRVACPRQED
ADALAEIARKSKIPVIADIHFQPKYIFAAIDAGCAAVRVNPGNIKEFDGRVKEVAKAAGDAGIPIRIGVNAGSLDPRILG
KYGKATPEALVESALWEAGLFEEHGFGDIKISVKHNDPVIMVAAYELLAAQCDYPLHLGVTEAGPAFQGTIKSAVAFGAL
LSKGIGDTIRVSLSAPPAEEVKVGNQILESLNLRPRSLEIVSCPSCGRAQVDVYTLANAVSAGLEGLDVPLRVAVMGCVV
NGPGEAREADLGVASGNGKGQIFVKGEVIKTVPEALIVETLIDEAMRLAEQMGLENGAGIGSDGTPTGQPVVTVS

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=326, Percent_Identity=46.0122699386503, Blast_Score=298, Evalue=3e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 40945; Mature: 40945

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTGLGMPSLPGAPPPTLAPRRQTRQLMVGSVGVGSDSPVSVQSMCTTKTHDVNSTLQQI
CCCCCCCCCCCCCCCCCCCCCHHHHHHHEEECCCCCCCCCCHHHHHCCCCCCHHHHHHHH
AELTASGCDIVRVACPRQEDADALAEIARKSKIPVIADIHFQPKYIFAAIDAGCAAVRVN
HHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCEEEEEC
PGNIKEFDGRVKEVAKAAGDAGIPIRIGVNAGSLDPRILGKYGKATPEALVESALWEAGL
CCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCC
FEEHGFGDIKISVKHNDPVIMVAAYELLAAQCDYPLHLGVTEAGPAFQGTIKSAVAFGAL
CCCCCCCEEEEEEECCCCEEEEEHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHH
LSKGIGDTIRVSLSAPPAEEVKVGNQILESLNLRPRSLEIVSCPSCGRAQVDVYTLANAV
HHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEHHHHHHHHH
SAGLEGLDVPLRVAVMGCVVNGPGEAREADLGVASGNGKGQIFVKGEVIKTVPEALIVET
HHCCCCCCCHHEEEEEEEEECCCCCCCCCCCEEECCCCCEEEEECCHHHHHHHHHHHHHH
LIDEAMRLAEQMGLENGAGIGSDGTPTGQPVVTVS
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEC
>Mature Secondary Structure
MMTGLGMPSLPGAPPPTLAPRRQTRQLMVGSVGVGSDSPVSVQSMCTTKTHDVNSTLQQI
CCCCCCCCCCCCCCCCCCCCCHHHHHHHEEECCCCCCCCCCHHHHHCCCCCCHHHHHHHH
AELTASGCDIVRVACPRQEDADALAEIARKSKIPVIADIHFQPKYIFAAIDAGCAAVRVN
HHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCEEEEEC
PGNIKEFDGRVKEVAKAAGDAGIPIRIGVNAGSLDPRILGKYGKATPEALVESALWEAGL
CCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCC
FEEHGFGDIKISVKHNDPVIMVAAYELLAAQCDYPLHLGVTEAGPAFQGTIKSAVAFGAL
CCCCCCCEEEEEEECCCCEEEEEHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHH
LSKGIGDTIRVSLSAPPAEEVKVGNQILESLNLRPRSLEIVSCPSCGRAQVDVYTLANAV
HHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEHHHHHHHHH
SAGLEGLDVPLRVAVMGCVVNGPGEAREADLGVASGNGKGQIFVKGEVIKTVPEALIVET
HHCCCCCCCHHEEEEEEEEECCCCCCCCCCCEEECCCCCEEEEECCHHHHHHHHHHHHHH
LIDEAMRLAEQMGLENGAGIGSDGTPTGQPVVTVS
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA