Click here to switch to the map view.

The map label for this gene is truB

Identifier: 169630198

GI number: 169630198

Start: 3158360

End: 3159256

Strand: Reverse

Name: truB

Synonym: MAB_3116c

Alternate gene names: 169630198

Gene position: 3159256-3158360 (Counterclockwise)

Preceding gene: 169630199

Following gene: 169630191

Centisome position: 62.35

GC content: 65.55

Gene sequence:

>897_bases
GTGACCGATCCGCTGAGCCGGGCGGGCCTGGTGATCGTTGACAAGCCCGCCGGAATGACGAGTCATGACGTGGTGTCGCG
TTGTCGCAGAATATTTTCCACCCGTAAGGTGGGGCATGCCGGGACCCTCGACCCGATGGCTACCGGAGTACTGGTCATTG
GGATCGAGCGGGCCACCAAGATCATGGGCCTACTCACGTTGACCACCAAGTCGTACGCGGCGACTATCCGATTCGGTCAG
AGCACCAGCACCGATGATGCCGAAGGGGAGGTGCTGCAAACGATTTCGGCATCGCACCTCACCGAGGTGGATATCGAGGC
CGCCGTGGCCGGACTGCGTGGAGATATCGCGCAGGTGCCGTCCTCGGTGAGTGCGATCAAGGTCGACGGACAGCGCGCGT
ACAAGCTGGCACGCGAGGGGCAGTCGGTCGAGCTGGCCGCGCGTCCGGTGCGTATCTCTCGCTTCGACGTGTTGGCGGTG
CGAGGCTCGGGTGAGGACCTGATCGATGTGGACGTGGAAATCGACTGCAGCTCGGGGACCTATATTCGTGCGCTCGCCCG
GGATGCGGGGGCCGCGCTCGGTGTGGGCGGACACCTGACGGCACTGCGGCGCACCAGGGTTGGTGATTTCGGGCTGGATC
GAGCCCGCACGCTCGAGGAGCTGGCCGATGGCGCGACGCTAACCCTGGACCTGGATTCCGCGTGCCGGCAAGGCTTTCCC
CGGCGTGACCTGGACGCCGCCGAGGTGGATGCGGTGCGTAACGGTCGGCCACTGGCGCCTGCGGGGATCGAAGGCACCTA
CGCGGCGGTGGATGCCGATGATCGCGTCATCGCGCTGCTGGCGGACAAGGGATCACGCACCACATCGGTCGTGGTGTTGC
GGCCCGCGAATTTGTAG

Upstream 100 bases:

>100_bases
TCTTGGTTGACGGCAGGGCCAACGACGGCACCGTGCTGTCGGCCTTTGACGGCCGATGGATCATCGACAAGGGTTTGATC
TTGACCGCGATTGTGGTGCC

Downstream 100 bases:

>100_bases
CGGCTACCCGAAAACGGCCTTGTGCCACCACCATTTGGGGTCGCTGAACCAAGGTCCGAAGTTGCCCGAATTCAGAACTC
GCAGTTCTGTCATGTCGATG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MTDPLSRAGLVIVDKPAGMTSHDVVSRCRRIFSTRKVGHAGTLDPMATGVLVIGIERATKIMGLLTLTTKSYAATIRFGQ
STSTDDAEGEVLQTISASHLTEVDIEAAVAGLRGDIAQVPSSVSAIKVDGQRAYKLAREGQSVELAARPVRISRFDVLAV
RGSGEDLIDVDVEIDCSSGTYIRALARDAGAALGVGGHLTALRRTRVGDFGLDRARTLEELADGATLTLDLDSACRQGFP
RRDLDAAEVDAVRNGRPLAPAGIEGTYAAVDADDRVIALLADKGSRTTSVVVLRPANL

Sequences:

>Translated_298_residues
MTDPLSRAGLVIVDKPAGMTSHDVVSRCRRIFSTRKVGHAGTLDPMATGVLVIGIERATKIMGLLTLTTKSYAATIRFGQ
STSTDDAEGEVLQTISASHLTEVDIEAAVAGLRGDIAQVPSSVSAIKVDGQRAYKLAREGQSVELAARPVRISRFDVLAV
RGSGEDLIDVDVEIDCSSGTYIRALARDAGAALGVGGHLTALRRTRVGDFGLDRARTLEELADGATLTLDLDSACRQGFP
RRDLDAAEVDAVRNGRPLAPAGIEGTYAAVDADDRVIALLADKGSRTTSVVVLRPANL
>Mature_297_residues
TDPLSRAGLVIVDKPAGMTSHDVVSRCRRIFSTRKVGHAGTLDPMATGVLVIGIERATKIMGLLTLTTKSYAATIRFGQS
TSTDDAEGEVLQTISASHLTEVDIEAAVAGLRGDIAQVPSSVSAIKVDGQRAYKLAREGQSVELAARPVRISRFDVLAVR
GSGEDLIDVDVEIDCSSGTYIRALARDAGAALGVGGHLTALRRTRVGDFGLDRARTLEELADGATLTLDLDSACRQGFPR
RDLDAAEVDAVRNGRPLAPAGIEGTYAAVDADDRVIALLADKGSRTTSVVVLRPANL

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=226, Percent_Identity=34.5132743362832, Blast_Score=118, Evalue=6e-27,
Organism=Homo sapiens, GI215599015, Length=222, Percent_Identity=29.7297297297297, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI4503337, Length=237, Percent_Identity=29.1139240506329, Blast_Score=78, Evalue=8e-15,
Organism=Escherichia coli, GI2367200, Length=219, Percent_Identity=40.1826484018265, Blast_Score=166, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17553978, Length=213, Percent_Identity=28.169014084507, Blast_Score=84, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6324037, Length=132, Percent_Identity=39.3939393939394, Blast_Score=93, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323204, Length=230, Percent_Identity=29.1304347826087, Blast_Score=89, Evalue=7e-19,
Organism=Drosophila melanogaster, GI281364189, Length=203, Percent_Identity=28.5714285714286, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281364187, Length=203, Percent_Identity=28.5714285714286, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281364185, Length=203, Percent_Identity=28.5714285714286, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281364183, Length=203, Percent_Identity=28.5714285714286, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI62471759, Length=203, Percent_Identity=28.5714285714286, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17975520, Length=203, Percent_Identity=28.5714285714286, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_MYCA9 (B1MD72)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001703847.1
- ProteinModelPortal:   B1MD72
- SMR:   B1MD72
- EnsemblBacteria:   EBMYCT00000006196
- GeneID:   5965621
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_3116c
- GeneTree:   EBGT00050000016018
- HOGENOM:   HBG397258
- OMA:   YPAHMSK
- ProtClustDB:   PRK03287
- BioCyc:   MABS561007:MAB_3116C-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR015225
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09142 TruB_C; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 31362; Mature: 31231

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: ACT_SITE 44-44

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDPLSRAGLVIVDKPAGMTSHDVVSRCRRIFSTRKVGHAGTLDPMATGVLVIGIERATK
CCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCEEEEEEHHHHH
IMGLLTLTTKSYAATIRFGQSTSTDDAEGEVLQTISASHLTEVDIEAAVAGLRGDIAQVP
HHHEEEEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCHHHCC
SSVSAIKVDGQRAYKLAREGQSVELAARPVRISRFDVLAVRGSGEDLIDVDVEIDCSSGT
CCCEEEEECCHHHHHHHHCCCCEEEEECCEEEEEEEEEEEECCCCCEEEEEEEEECCCCC
YIRALARDAGAALGVGGHLTALRRTRVGDFGLDRARTLEELADGATLTLDLDSACRQGFP
EEEEEHHHCCCEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEECCHHHHCCCC
RRDLDAAEVDAVRNGRPLAPAGIEGTYAAVDADDRVIALLADKGSRTTSVVVLRPANL
CCCCCHHHHHHHHCCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCCEEEEEEECCCC
>Mature Secondary Structure 
TDPLSRAGLVIVDKPAGMTSHDVVSRCRRIFSTRKVGHAGTLDPMATGVLVIGIERATK
CCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCEEEEEEHHHHH
IMGLLTLTTKSYAATIRFGQSTSTDDAEGEVLQTISASHLTEVDIEAAVAGLRGDIAQVP
HHHEEEEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCHHHCC
SSVSAIKVDGQRAYKLAREGQSVELAARPVRISRFDVLAVRGSGEDLIDVDVEIDCSSGT
CCCEEEEECCHHHHHHHHCCCCEEEEECCEEEEEEEEEEEECCCCCEEEEEEEEECCCCC
YIRALARDAGAALGVGGHLTALRRTRVGDFGLDRARTLEELADGATLTLDLDSACRQGFP
EEEEEHHHCCCEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEECCHHHHCCCC
RRDLDAAEVDAVRNGRPLAPAGIEGTYAAVDADDRVIALLADKGSRTTSVVVLRPANL
CCCCCHHHHHHHHCCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA