Definition Mycobacterium abscessus ATCC 19977 chromosome chromosome 1, complete sequence.
Accession NC_010397
Length 5,067,172

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The map label for this gene is pnp

Identifier: 169630188

GI number: 169630188

Start: 3149848

End: 3152115

Strand: Reverse

Name: pnp

Synonym: MAB_3106c

Alternate gene names: 169630188

Gene position: 3152115-3149848 (Counterclockwise)

Preceding gene: 169630189

Following gene: 169630187

Centisome position: 62.21

GC content: 65.48

Gene sequence:

>2268_bases
ATGTCTGTTACAGAAATTGAAGAAGGCATCTTCGAGTCCACCGCGACCATCGACAACGGGAGCTTCGGCACCCGCACCAT
CCGGTTCGAGACGGGGCGCCTGGCCCTGCAGGCCGCCGGTTCGGTCGTCGCGTACCTGGACGACGAGACGATGCTGCTGT
CGACCACGGCCGCCAGCAAGACTCCGAAGGATCACTTCGACTTCTTCCCGCTGACCGTCGACGTCGAGGAGCGGATGTAC
GCCGCGGGCCGCATCCCCGGCTCGTTCTTCCGTCGTGAGGGTCGTCCCTCCACCGACGCCATTTTGACTTGCCGTCTGAT
CGACCGGCCGCTGCGCCCGTCGTTCGTGAGCGGGCTGCGTAACGAGATCCAGGTCGTGGTGACGGTGCTGAGCCTGAACC
CCGCCGATCTGTACGACGTGCTGGCCATCAATGCGGCGTCCGCCTCCACTCAGCTTGCCGGACTCCCGTTCTCGGGCCCG
GTTGGCGGAGTGCGAGTCGCCCTGATCGAGGGACAGTGGGTGGCGTTCCCGACCGTCGAGCAGCTGGAGAAGGCTGTGTT
CGACATGGTGGTCGCGGGCCGCAAAGTAGGCACCGCCGGTGATTCCGACGTGGCGATCATGATGGTCGAGGCCGAGGCCA
CTGACAACGTCATCGCATTGATCGACGGTGGCGCCGGTGCGCCCACCGAAACCGTTGTCGCCGAGGGCCTCGAGGCGGCC
AAGCCGTTCATCGCTGCCCTGTGCACCGCGCAGGAGGAGCTGGCCGGCAGCGCTGCCAAGCCCACCGGCGAGTTCCCGCT
GTTCCCGGACTACCAGGACGACGTCTACGCCGCCGTCGCGGCTGTGGCCACCGAGCCGCTGTCGCAGGCGCTCTCGATCG
CGGGCAAGGCCGAGCGTGATGAGAAGACCGACGAGATCAAGGTCGACGTGCTGGGCAGGCTCCAGGAGGGCTTCGACGGC
CGTGAGAAGGAAATCGGCGCCGCGTTCCGCTCGCTCACCAAGAAGCTTGTGCGCCAGCGCATCCTGAAGGATCAGTTCCG
CATCGATGGCCGCGGTGTCACCGACATTCGCGCCCTGTCAGCCGAGGTCGCCGTGATCCCGCGTGCCCACGGCAGTGCCC
TGTTCGAGCGTGGCGAGACCCAGATCATGGGTGTGACCACCCTGGATATGATCAAGATGGCTCAGCAGGTCGATTCGCTC
GGACCCGAGACCACCAAGCGCTACATGCACCACTACAACTTCCCGCCCTTCTCCACCGGTGAGACCGGCCGCGTCGGTTC
GCCGAAGCGTCGCGAGATAGGCCACGGCGCCCTCGCCGAGCGGGCGCTCATCCCGGTGCTCCCGAGCATTGAGGAGTTCC
CCTACGCGATCCGTCAGGTCTCGGAGGCGCTGGGCTCCAACGGTTCTACCTCGATGGGTTCGGTGTGTGCTTCCACTCTG
GCGCTGCTGAACGCCGGCGTGCCGCTGAAGGCGCCCGTCGCCGGTATCGCCATGGGTCTGGTGTCCGATGACATCGAACT
CGCAGACGGCACCGCCGAGCGTCGCTTCGTCGCGCTCACCGATATCCTGGGTGCCGAGGATGCCTTCGGCGACATGGACT
TCAAGGTCGCCGGCACCAAGGACTTCGTCACCGCCCTGCAGCTGGACACCAAGCTCGACGGCATCCCGTCGCAGGTGCTG
GCCGCCGCGTTGTCGCAGGCCAAAGACGCCCGCACCACCATCCTCGAGGTGATGGCCGAGGCGATCGACGCTCCCGACGA
GATGAGCCCGTACGCGCCGCGTATCACCACCATCAAGGTGCCGGTCGACAAGATCGGTGAGGTGATTGGGCCCAAGGGCA
AGATGATCAACTCCATCACCGAGGAGACCGGCGCGAACATCTCCATCGAGGATGACGGCACCGTGTTCGTCGGTGCTGCC
GACGGCGCTTCGGCGCAGGCCGCGATCGACAAGATCAACGCCATCGCCAACCCGCAGTTGCCCAAGATCGGCGAGCGTTT
CCTCGGAACGGTGGTGAAGACCACTGACTTTGGAGCTTTCGTTTCCCTCTTGCCTGGCCGCGACGGCCTGGTGCACATCA
GCAAGCTCGGTAAGGGCAAGCGGATTGCCAAGGTTGAAGACGTGGTCAAGGTCGGCGACAAGATCCAGGTGGAGATCGCC
GATATCGACAACCGCGGCAAGATCTCGCTGGTCCCGGTCGGCGAAGAGGATGCCGCGGAAGCTCCCGCACCCGCGGAGGC
CCAGCCGGCTGATGCCGTCACCCAGTAA

Upstream 100 bases:

>100_bases
GGTCCACTTCGCGCTGGTACAACCGCGCGCTGACCGTGTGATGGCACGCATGCCCACTACAGCGAACACGCTGAATAACT
GAGAGGTAAATCGGACACCT

Downstream 100 bases:

>100_bases
GGTCCCGAGCGCAGGGTCCGAGTCTCTTCGCGCAAGCGGCTCATCGGTACGCCGCACGGTTCTTCCAGGTGGGCTCCGAG
TAGTCACCGAGGCCATGCCC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 755; Mature: 754

Protein sequence:

>755_residues
MSVTEIEEGIFESTATIDNGSFGTRTIRFETGRLALQAAGSVVAYLDDETMLLSTTAASKTPKDHFDFFPLTVDVEERMY
AAGRIPGSFFRREGRPSTDAILTCRLIDRPLRPSFVSGLRNEIQVVVTVLSLNPADLYDVLAINAASASTQLAGLPFSGP
VGGVRVALIEGQWVAFPTVEQLEKAVFDMVVAGRKVGTAGDSDVAIMMVEAEATDNVIALIDGGAGAPTETVVAEGLEAA
KPFIAALCTAQEELAGSAAKPTGEFPLFPDYQDDVYAAVAAVATEPLSQALSIAGKAERDEKTDEIKVDVLGRLQEGFDG
REKEIGAAFRSLTKKLVRQRILKDQFRIDGRGVTDIRALSAEVAVIPRAHGSALFERGETQIMGVTTLDMIKMAQQVDSL
GPETTKRYMHHYNFPPFSTGETGRVGSPKRREIGHGALAERALIPVLPSIEEFPYAIRQVSEALGSNGSTSMGSVCASTL
ALLNAGVPLKAPVAGIAMGLVSDDIELADGTAERRFVALTDILGAEDAFGDMDFKVAGTKDFVTALQLDTKLDGIPSQVL
AAALSQAKDARTTILEVMAEAIDAPDEMSPYAPRITTIKVPVDKIGEVIGPKGKMINSITEETGANISIEDDGTVFVGAA
DGASAQAAIDKINAIANPQLPKIGERFLGTVVKTTDFGAFVSLLPGRDGLVHISKLGKGKRIAKVEDVVKVGDKIQVEIA
DIDNRGKISLVPVGEEDAAEAPAPAEAQPADAVTQ

Sequences:

>Translated_755_residues
MSVTEIEEGIFESTATIDNGSFGTRTIRFETGRLALQAAGSVVAYLDDETMLLSTTAASKTPKDHFDFFPLTVDVEERMY
AAGRIPGSFFRREGRPSTDAILTCRLIDRPLRPSFVSGLRNEIQVVVTVLSLNPADLYDVLAINAASASTQLAGLPFSGP
VGGVRVALIEGQWVAFPTVEQLEKAVFDMVVAGRKVGTAGDSDVAIMMVEAEATDNVIALIDGGAGAPTETVVAEGLEAA
KPFIAALCTAQEELAGSAAKPTGEFPLFPDYQDDVYAAVAAVATEPLSQALSIAGKAERDEKTDEIKVDVLGRLQEGFDG
REKEIGAAFRSLTKKLVRQRILKDQFRIDGRGVTDIRALSAEVAVIPRAHGSALFERGETQIMGVTTLDMIKMAQQVDSL
GPETTKRYMHHYNFPPFSTGETGRVGSPKRREIGHGALAERALIPVLPSIEEFPYAIRQVSEALGSNGSTSMGSVCASTL
ALLNAGVPLKAPVAGIAMGLVSDDIELADGTAERRFVALTDILGAEDAFGDMDFKVAGTKDFVTALQLDTKLDGIPSQVL
AAALSQAKDARTTILEVMAEAIDAPDEMSPYAPRITTIKVPVDKIGEVIGPKGKMINSITEETGANISIEDDGTVFVGAA
DGASAQAAIDKINAIANPQLPKIGERFLGTVVKTTDFGAFVSLLPGRDGLVHISKLGKGKRIAKVEDVVKVGDKIQVEIA
DIDNRGKISLVPVGEEDAAEAPAPAEAQPADAVTQ
>Mature_754_residues
SVTEIEEGIFESTATIDNGSFGTRTIRFETGRLALQAAGSVVAYLDDETMLLSTTAASKTPKDHFDFFPLTVDVEERMYA
AGRIPGSFFRREGRPSTDAILTCRLIDRPLRPSFVSGLRNEIQVVVTVLSLNPADLYDVLAINAASASTQLAGLPFSGPV
GGVRVALIEGQWVAFPTVEQLEKAVFDMVVAGRKVGTAGDSDVAIMMVEAEATDNVIALIDGGAGAPTETVVAEGLEAAK
PFIAALCTAQEELAGSAAKPTGEFPLFPDYQDDVYAAVAAVATEPLSQALSIAGKAERDEKTDEIKVDVLGRLQEGFDGR
EKEIGAAFRSLTKKLVRQRILKDQFRIDGRGVTDIRALSAEVAVIPRAHGSALFERGETQIMGVTTLDMIKMAQQVDSLG
PETTKRYMHHYNFPPFSTGETGRVGSPKRREIGHGALAERALIPVLPSIEEFPYAIRQVSEALGSNGSTSMGSVCASTLA
LLNAGVPLKAPVAGIAMGLVSDDIELADGTAERRFVALTDILGAEDAFGDMDFKVAGTKDFVTALQLDTKLDGIPSQVLA
AALSQAKDARTTILEVMAEAIDAPDEMSPYAPRITTIKVPVDKIGEVIGPKGKMINSITEETGANISIEDDGTVFVGAAD
GASAQAAIDKINAIANPQLPKIGERFLGTVVKTTDFGAFVSLLPGRDGLVHISKLGKGKRIAKVEDVVKVGDKIQVEIAD
IDNRGKISLVPVGEEDAAEAPAPAEAQPADAVTQ

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=720, Percent_Identity=35.6944444444444, Blast_Score=389, Evalue=1e-108,
Organism=Escherichia coli, GI145693187, Length=714, Percent_Identity=45.9383753501401, Blast_Score=578, Evalue=1e-166,
Organism=Caenorhabditis elegans, GI115534063, Length=741, Percent_Identity=33.1983805668016, Blast_Score=315, Evalue=8e-86,
Organism=Drosophila melanogaster, GI281362905, Length=707, Percent_Identity=35.5021216407355, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24651641, Length=707, Percent_Identity=35.5021216407355, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24651643, Length=707, Percent_Identity=35.5021216407355, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI161079377, Length=652, Percent_Identity=35.5828220858896, Blast_Score=364, Evalue=1e-100,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_MYCA9 (B1MD62)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001703837.1
- ProteinModelPortal:   B1MD62
- SMR:   B1MD62
- EnsemblBacteria:   EBMYCT00000004913
- GeneID:   5965612
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_3106c
- GeneTree:   EBGT00050000017412
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- BioCyc:   MABS561007:MAB_3106C-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR014069
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591
- TIGRFAMs:   TIGR02696

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 79794; Mature: 79662

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MSVTEIEEGIFESTATIDNGSFGTRTIRFETGRLALQAAGSVVAYLDDETMLLSTTAASK
CCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEEECCCEEEEECCCEEEEEECCCCC
TPKDHFDFFPLTVDVEERMYAAGRIPGSFFRREGRPSTDAILTCRLIDRPLRPSFVSGLR
CCCCCCCEEEEEEEHHHHHHHHCCCCHHHHHCCCCCCHHHEEEEHHHCCCCCHHHHHHHH
NEIQVVVTVLSLNPADLYDVLAINAASASTQLAGLPFSGPVGGVRVALIEGQWVAFPTVE
HHHEEEEEEEECCCHHHHHHHEEECCCCCCEEECCCCCCCCCCEEEEEEECCEEECCCHH
QLEKAVFDMVVAGRKVGTAGDSDVAIMMVEAEATDNVIALIDGGAGAPTETVVAEGLEAA
HHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHH
KPFIAALCTAQEELAGSAAKPTGEFPLFPDYQDDVYAAVAAVATEPLSQALSIAGKAERD
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
EKTDEIKVDVLGRLQEGFDGREKEIGAAFRSLTKKLVRQRILKDQFRIDGRGVTDIRALS
CCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHC
AEVAVIPRAHGSALFERGETQIMGVTTLDMIKMAQQVDSLGPETTKRYMHHYNFPPFSTG
CCEEEEECCCCHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCC
ETGRVGSPKRREIGHGALAERALIPVLPSIEEFPYAIRQVSEALGSNGSTSMGSVCASTL
CCCCCCCCHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ALLNAGVPLKAPVAGIAMGLVSDDIELADGTAERRFVALTDILGAEDAFGDMDFKVAGTK
HHHHCCCCCCCCHHHHHHHHHCCCCEECCCCCCCEEEEHHHHHCCCCCCCCCCEEEECCH
DFVTALQLDTKLDGIPSQVLAAALSQAKDARTTILEVMAEAIDAPDEMSPYAPRITTIKV
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEC
PVDKIGEVIGPKGKMINSITEETGANISIEDDGTVFVGAADGASAQAAIDKINAIANPQL
CHHHHHHHHCCCCHHHHHHHHHHCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHCCCCC
PKIGERFLGTVVKTTDFGAFVSLLPGRDGLVHISKLGKGKRIAKVEDVVKVGDKIQVEIA
HHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEEEE
DIDNRGKISLVPVGEEDAAEAPAPAEAQPADAVTQ
ECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SVTEIEEGIFESTATIDNGSFGTRTIRFETGRLALQAAGSVVAYLDDETMLLSTTAASK
CCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEEECCCEEEEECCCEEEEEECCCCC
TPKDHFDFFPLTVDVEERMYAAGRIPGSFFRREGRPSTDAILTCRLIDRPLRPSFVSGLR
CCCCCCCEEEEEEEHHHHHHHHCCCCHHHHHCCCCCCHHHEEEEHHHCCCCCHHHHHHHH
NEIQVVVTVLSLNPADLYDVLAINAASASTQLAGLPFSGPVGGVRVALIEGQWVAFPTVE
HHHEEEEEEEECCCHHHHHHHEEECCCCCCEEECCCCCCCCCCEEEEEEECCEEECCCHH
QLEKAVFDMVVAGRKVGTAGDSDVAIMMVEAEATDNVIALIDGGAGAPTETVVAEGLEAA
HHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHH
KPFIAALCTAQEELAGSAAKPTGEFPLFPDYQDDVYAAVAAVATEPLSQALSIAGKAERD
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
EKTDEIKVDVLGRLQEGFDGREKEIGAAFRSLTKKLVRQRILKDQFRIDGRGVTDIRALS
CCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHC
AEVAVIPRAHGSALFERGETQIMGVTTLDMIKMAQQVDSLGPETTKRYMHHYNFPPFSTG
CCEEEEECCCCHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCC
ETGRVGSPKRREIGHGALAERALIPVLPSIEEFPYAIRQVSEALGSNGSTSMGSVCASTL
CCCCCCCCHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ALLNAGVPLKAPVAGIAMGLVSDDIELADGTAERRFVALTDILGAEDAFGDMDFKVAGTK
HHHHCCCCCCCCHHHHHHHHHCCCCEECCCCCCCEEEEHHHHHCCCCCCCCCCEEEECCH
DFVTALQLDTKLDGIPSQVLAAALSQAKDARTTILEVMAEAIDAPDEMSPYAPRITTIKV
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEC
PVDKIGEVIGPKGKMINSITEETGANISIEDDGTVFVGAADGASAQAAIDKINAIANPQL
CHHHHHHHHCCCCHHHHHHHHHHCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHCCCCC
PKIGERFLGTVVKTTDFGAFVSLLPGRDGLVHISKLGKGKRIAKVEDVVKVGDKIQVEIA
HHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEEEE
DIDNRGKISLVPVGEEDAAEAPAPAEAQPADAVTQ
ECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA