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The map label for this gene is folA [H]

Identifier: 169630172

GI number: 169630172

Start: 3134359

End: 3134850

Strand: Reverse

Name: folA [H]

Synonym: MAB_3090c

Alternate gene names: 169630172

Gene position: 3134850-3134359 (Counterclockwise)

Preceding gene: 169630173

Following gene: 169630171

Centisome position: 61.87

GC content: 64.43

Gene sequence:

>492_bases
ATGACGGGAACCATCGGGCTGATCTGGGCGCAAACCCGAGCCGGCGTCATCGGCGCGGACGGTGCCATTCCCTGGCGGCT
ACCCGAAGACCAGGCACGTTTCAAGCGAATCACCATGGGGCACACAGTGATCATGGGGCGCAAGACCTGGGAGTCGCTGC
CCGGTAGTGTCCGTCCTTTGCCGGGTCGGCCCAATATCGTGCTGACCAGGGATGCCCTGTTCGAGCCCGACGGGGCATTG
GCCGTCGGCTGCGCGGACGCGGCCCTGGCGGCTTCCGACGAGGCGCCCTGGGTCATCGGCGGCGGAGAGATCTATCGGCT
GTTTCTGCCGTTGGCACAACGCTGCGAAGTGACGGTGGTGGAGGCCGACGTGCCGGGTGACGCGCTTGCTCCGGAACTCG
GAGAAGGCTGGGTTGTCGAGGAAAATGACTGGCAGACAAGCGAGTCCGGCTTGCGCTATCAATTCCTGAGTTATCGGAAA
GTCGACGGGTGA

Upstream 100 bases:

>100_bases
TTGTTCTGGCGCAGCGTGATTCGATATTCGACTACCGATACGAGGACATCACCGTCGAGAACTACCAGCATCACGCCGCC
ATCAAGGCGCCGGTCGCGGT

Downstream 100 bases:

>100_bases
GTGCCCCGGGTGCGGCAGGGGTGCCCAAGGTGCTGCTGCTTGCGGCGATAGTGCTGCTCGTTCTGACCGGCTGTGCGCGC
ACCGTGGAGGGAACGGCGTC

Product: dihydrofolate reductase DfrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 163; Mature: 162

Protein sequence:

>163_residues
MTGTIGLIWAQTRAGVIGADGAIPWRLPEDQARFKRITMGHTVIMGRKTWESLPGSVRPLPGRPNIVLTRDALFEPDGAL
AVGCADAALAASDEAPWVIGGGEIYRLFLPLAQRCEVTVVEADVPGDALAPELGEGWVVEENDWQTSESGLRYQFLSYRK
VDG

Sequences:

>Translated_163_residues
MTGTIGLIWAQTRAGVIGADGAIPWRLPEDQARFKRITMGHTVIMGRKTWESLPGSVRPLPGRPNIVLTRDALFEPDGAL
AVGCADAALAASDEAPWVIGGGEIYRLFLPLAQRCEVTVVEADVPGDALAPELGEGWVVEENDWQTSESGLRYQFLSYRK
VDG
>Mature_162_residues
TGTIGLIWAQTRAGVIGADGAIPWRLPEDQARFKRITMGHTVIMGRKTWESLPGSVRPLPGRPNIVLTRDALFEPDGALA
VGCADAALAASDEAPWVIGGGEIYRLFLPLAQRCEVTVVEADVPGDALAPELGEGWVVEENDWQTSESGLRYQFLSYRKV
DG

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=188, Percent_Identity=32.4468085106383, Blast_Score=76, Evalue=1e-14,
Organism=Escherichia coli, GI1786233, Length=150, Percent_Identity=42.6666666666667, Blast_Score=100, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17505899, Length=185, Percent_Identity=33.5135135135135, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6324810, Length=123, Percent_Identity=30.8943089430894, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 17647; Mature: 17516

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGTIGLIWAQTRAGVIGADGAIPWRLPEDQARFKRITMGHTVIMGRKTWESLPGSVRPL
CCCCEEEEEECCCCCEEECCCCCCCCCCCHHHHHEEEECCEEEEEECCHHHHCCCCCCCC
PGRPNIVLTRDALFEPDGALAVGCADAALAASDEAPWVIGGGEIYRLFLPLAQRCEVTVV
CCCCCEEEEECCEECCCCCEEEECCCCEEECCCCCCEEEECHHHHHEEHHHHCCCEEEEE
EADVPGDALAPELGEGWVVEENDWQTSESGLRYQFLSYRKVDG
EECCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEEEEEECCC
>Mature Secondary Structure 
TGTIGLIWAQTRAGVIGADGAIPWRLPEDQARFKRITMGHTVIMGRKTWESLPGSVRPL
CCCEEEEEECCCCCEEECCCCCCCCCCCHHHHHEEEECCEEEEEECCHHHHCCCCCCCC
PGRPNIVLTRDALFEPDGALAVGCADAALAASDEAPWVIGGGEIYRLFLPLAQRCEVTVV
CCCCCEEEEECCEECCCCCEEEECCCCEEECCCCCCEEEECHHHHHEEHHHHCCCEEEEE
EADVPGDALAPELGEGWVVEENDWQTSESGLRYQFLSYRKVDG
EECCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]