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The map label for this gene is hdhA [C]

Identifier: 169630153

GI number: 169630153

Start: 3116938

End: 3117876

Strand: Direct

Name: hdhA [C]

Synonym: MAB_3070

Alternate gene names: 169630153

Gene position: 3116938-3117876 (Clockwise)

Preceding gene: 169630147

Following gene: 169630155

Centisome position: 61.51

GC content: 64.86

Gene sequence:

>939_bases
ATGCCGCGCACACCGTATGACGTCGACATACCCGACCTGTCAGGACAACGCGCGGTGGTCACCGGCGCCAGCGACGGGAT
CGGGCTGGAAATCGCAATGAAACTCGCCGGGGCGGGCGCCGACGTGTTCATGCCAGTACGAAACCTGCGTAAAGGGCAAG
CTGCCGTCTCCAGGATTCGCGCACTGCACCCCCGTGCGCACCTGACACTGGCCGAACTCGATTTGTCGTCATTGGTATCC
ATCGCTGACCTCGGTGAAAAACTTTGTGCTGAAGGAACTCCCATCCATCTGCTGATCAACAACGCAGGTGTGATGACCCC
ACCCGAGAGACAGACCACTGCCGACGGGTTCGAACTGCAATTCGGCACCAACCATCTCGGCCACTTCGCACTCACCGGTC
ACCTCTTGCCGCTCCTGCGGGCCGGGCGGGCGCGGGTGACGTCGCACACCAGCATCGCGGCGCGCAGCGGCGAGATCAAT
TGGGATGACCTGAATTGGGAGCGCGGTTACGACGGTATGCGCGCCTACCGGCAGTCCAAGATCGCATGCGGGCTGTTCGG
TCTGGAGCTCAGCCGGCTGAGCAACACGGCCGGCTGGGGAATCACGAGCAATATCGCCCATCCGGGAGTCGCCCCCACCA
GCCTGCTGGCTGCGCGCCCGGAAGTCGGGCGCACCAAGGACACCACTGAAGTCCGGATGATCCGCTGGCTCTCGGCGCGC
GGGCTCATCGTGGGCACCGTCGCAAGCGCCACACTGCCCGCCCTGATGGCCGCCGTGGCACCGGAGGCCACAGACGGCGG
TTTCTACGGTCCGCGCTGGCCCGGCAGCGCCGGCGGCCCTCCAGGACCGCAGAAACTCTGGAAGCCGTTGCACAGCATCA
GGAATGCGTCACGACTGTGGCAGGCATCCCAGGAACTGACCGGTGTCACCTTCACCTGA

Upstream 100 bases:

>100_bases
TCCACGGATCGTCAGTCCGTGGATACGCAGCACGCCCGCGGGGACGCTCGTCTCAGATGCCTCGATACCGGCACAACACG
CCAACACCAGGAGCAATCAC

Downstream 100 bases:

>100_bases
AAAACGCTAATCGACCGGCAGCACCATGACCGCCGATTCCAGCGGCGGGAATCTAGGGGGTTTTCGGCCGTGGGCGCAGC
GCCTGGACCAAATAGTCGAT

Product: short chain dehydrogenase

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MPRTPYDVDIPDLSGQRAVVTGASDGIGLEIAMKLAGAGADVFMPVRNLRKGQAAVSRIRALHPRAHLTLAELDLSSLVS
IADLGEKLCAEGTPIHLLINNAGVMTPPERQTTADGFELQFGTNHLGHFALTGHLLPLLRAGRARVTSHTSIAARSGEIN
WDDLNWERGYDGMRAYRQSKIACGLFGLELSRLSNTAGWGITSNIAHPGVAPTSLLAARPEVGRTKDTTEVRMIRWLSAR
GLIVGTVASATLPALMAAVAPEATDGGFYGPRWPGSAGGPPGPQKLWKPLHSIRNASRLWQASQELTGVTFT

Sequences:

>Translated_312_residues
MPRTPYDVDIPDLSGQRAVVTGASDGIGLEIAMKLAGAGADVFMPVRNLRKGQAAVSRIRALHPRAHLTLAELDLSSLVS
IADLGEKLCAEGTPIHLLINNAGVMTPPERQTTADGFELQFGTNHLGHFALTGHLLPLLRAGRARVTSHTSIAARSGEIN
WDDLNWERGYDGMRAYRQSKIACGLFGLELSRLSNTAGWGITSNIAHPGVAPTSLLAARPEVGRTKDTTEVRMIRWLSAR
GLIVGTVASATLPALMAAVAPEATDGGFYGPRWPGSAGGPPGPQKLWKPLHSIRNASRLWQASQELTGVTFT
>Mature_311_residues
PRTPYDVDIPDLSGQRAVVTGASDGIGLEIAMKLAGAGADVFMPVRNLRKGQAAVSRIRALHPRAHLTLAELDLSSLVSI
ADLGEKLCAEGTPIHLLINNAGVMTPPERQTTADGFELQFGTNHLGHFALTGHLLPLLRAGRARVTSHTSIAARSGEINW
DDLNWERGYDGMRAYRQSKIACGLFGLELSRLSNTAGWGITSNIAHPGVAPTSLLAARPEVGRTKDTTEVRMIRWLSARG
LIVGTVASATLPALMAAVAPEATDGGFYGPRWPGSAGGPPGPQKLWKPLHSIRNASRLWQASQELTGVTFT

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI225579078, Length=308, Percent_Identity=39.2857142857143, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI19923931, Length=263, Percent_Identity=39.9239543726236, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI186928839, Length=211, Percent_Identity=42.1800947867299, Blast_Score=134, Evalue=8e-32,
Organism=Homo sapiens, GI193804850, Length=300, Percent_Identity=34.6666666666667, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI166795268, Length=206, Percent_Identity=39.8058252427184, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI10190746, Length=217, Percent_Identity=36.8663594470046, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI7706523, Length=207, Percent_Identity=38.1642512077295, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI7705421, Length=231, Percent_Identity=28.1385281385281, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI146231950, Length=211, Percent_Identity=33.175355450237, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17532805, Length=213, Percent_Identity=34.2723004694836, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI193207620, Length=309, Percent_Identity=30.0970873786408, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17558006, Length=229, Percent_Identity=32.3144104803493, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17532791, Length=210, Percent_Identity=34.7619047619048, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI32565532, Length=210, Percent_Identity=35.2380952380952, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71992402, Length=208, Percent_Identity=34.1346153846154, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI72001364, Length=305, Percent_Identity=32.7868852459016, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17535059, Length=298, Percent_Identity=27.5167785234899, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17505416, Length=197, Percent_Identity=29.9492385786802, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI115533174, Length=296, Percent_Identity=25, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI115533172, Length=296, Percent_Identity=26.3513513513513, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324820, Length=173, Percent_Identity=32.9479768786127, Blast_Score=80, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28573296, Length=300, Percent_Identity=35, Blast_Score=148, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24586328, Length=204, Percent_Identity=41.1764705882353, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI22024069, Length=205, Percent_Identity=41.9512195121951, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI19921754, Length=205, Percent_Identity=40.4878048780488, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI161076371, Length=205, Percent_Identity=41.9512195121951, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI45554248, Length=299, Percent_Identity=35.1170568561873, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24640117, Length=299, Percent_Identity=35.1170568561873, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24656154, Length=205, Percent_Identity=37.5609756097561, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI19922628, Length=205, Percent_Identity=37.5609756097561, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI45553409, Length=199, Percent_Identity=33.1658291457286, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24647948, Length=199, Percent_Identity=33.1658291457286, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24647946, Length=199, Percent_Identity=33.1658291457286, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI20129347, Length=214, Percent_Identity=34.1121495327103, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24647931, Length=229, Percent_Identity=34.9344978165939, Blast_Score=82, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 33243; Mature: 33112

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRTPYDVDIPDLSGQRAVVTGASDGIGLEIAMKLAGAGADVFMPVRNLRKGQAAVSRIR
CCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHH
ALHPRAHLTLAELDLSSLVSIADLGEKLCAEGTPIHLLINNAGVMTPPERQTTADGFELQ
HHCCHHCEEHHHHCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCEEEE
FGTNHLGHFALTGHLLPLLRAGRARVTSHTSIAARSGEINWDDLNWERGYDGMRAYRQSK
ECCCCCCHHHHHHHHHHHHHCCCHHCCCCCEEEECCCCCCCCCCCHHCCCHHHHHHHHHH
IACGLFGLELSRLSNTAGWGITSNIAHPGVAPTSLLAARPEVGRTKDTTEVRMIRWLSAR
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHCC
GLIVGTVASATLPALMAAVAPEATDGGFYGPRWPGSAGGPPGPQKLWKPLHSIRNASRLW
CEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
QASQELTGVTFT
HHHHHCCCCCCC
>Mature Secondary Structure 
PRTPYDVDIPDLSGQRAVVTGASDGIGLEIAMKLAGAGADVFMPVRNLRKGQAAVSRIR
CCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHH
ALHPRAHLTLAELDLSSLVSIADLGEKLCAEGTPIHLLINNAGVMTPPERQTTADGFELQ
HHCCHHCEEHHHHCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCEEEE
FGTNHLGHFALTGHLLPLLRAGRARVTSHTSIAARSGEINWDDLNWERGYDGMRAYRQSK
ECCCCCCHHHHHHHHHHHHHCCCHHCCCCCEEEECCCCCCCCCCCHHCCCHHHHHHHHHH
IACGLFGLELSRLSNTAGWGITSNIAHPGVAPTSLLAARPEVGRTKDTTEVRMIRWLSAR
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHCC
GLIVGTVASATLPALMAAVAPEATDGGFYGPRWPGSAGGPPGPQKLWKPLHSIRNASRLW
CEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
QASQELTGVTFT
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.085 {glycochenodeoxycholic} 0.24 {taurochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383668 [H]