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The map label for this gene is miaB

Identifier: 169630131

GI number: 169630131

Start: 3100640

End: 3102163

Strand: Reverse

Name: miaB

Synonym: MAB_3048c

Alternate gene names: 169630131

Gene position: 3102163-3100640 (Counterclockwise)

Preceding gene: 169630132

Following gene: 169630130

Centisome position: 61.22

GC content: 65.03

Gene sequence:

>1524_bases
GTGACCAGTACTGCGACTTCCGGAGAATCGGCGGTCCGGACGTACCAGGTGCGCACCTACGGCTGCCAGATGAATGTGCA
CGACTCCGAGCGGGTGTCGGGCCTCCTGGATGCGGCCGGATACGTCAAGGCGCCCGAGGGCACCGACGCCGATATCGTCA
TCTTCAACACCTGCGCGGTGCGCGAGAACGCCGACAACAAGCTGTACGGAAACATCAGCCATCTGGCGCCCCGCAAGGCT
GCCAACCCGAACATGCAGATCGCCGTCGGTGGATGCCTCGCCCAGAAGGACCGCGAGGGCATGCTGGCCAAGGCCCCGTG
GGTCGATGTCGTCTTCGGTACCCACAACATCGGCTCACTGCCCGCATTGTTGGAGCGCGCCCGACACAACAATGAGGCGC
AGGTCGAGATCGTCGAGGCACTTGAGCACTTTCCGTCGGCCCTGCCTGCGACCCGTGAATCAGCCTATGCGGCCTGGGTA
TCGATCTCGGTCGGCTGCAACAACACCTGCACCTTCTGCATTGTTCCCTCGCTGCGCGGTAAAGAGATCGACCGCAGGCC
GGGAGACATCCTCGGTGAGGTCCAGGCGCTGGTAGACCAGGGAGTGCTGGAGGTGACGCTGCTGGGGCAGAACGTCAATG
CCTACGGCGTCAACTTCGCAGACCCCGAGATTCCCCGGGACCGTGGAGCTTTCGCGGAGCTGCTGCGTGCCTGCGGCCGT
ATCGACGGCCTGGAGCGGGTGCGATTCACCTCGCCGCACCCCGCCGAATTCACCGACGATGTCATCGAGGCAATGGCGCA
GACCCCGAATGTGTGCCCCCAGCTGCACATGCCGTTGCAATCAGGTTCGGACCGCATCCTGAAGGCGATGCGCCGTTCCT
ACCGGTCCGAGCGCTTCCTGTCCATCATCGACAAGGTGCGGTCGGCTATGCCGGACGCCGCCATCACCACCGACATCATC
GTCGGTTTCCCTGGTGAGACCGAAGAGGATTTCCAGCAGACCCTCGAGGTGGTCCGCCGCGCGCGTTTCTCCAGCGCCTT
TACGTTCCAGTACTCGATCCGTCCCGGCACCCCGGCCGCGAAGCTCCCCGACCAGCTGCCCAAAGCCGTTGTGCAGGAAC
GCTACGACCGGCTCATCGCGCTGCAGGAATCCGTCACCCTGGAGGAAAACCAGAAGCAGATCGGTCGCATGATCGAGGTG
CTGATCGCCACCGGGGAGGGCCGTAAGGACGGGGAGACAGCCCGGATGAGCGGGCGGGCCCGCGATGGCCGCCTCGTGCA
TTTCCGCCCGCAAGGACACGTCGACGGGGCACTGCGTCCCGGTGACATCATCACCGTCGACGTCACCGGTGCCGCACCGC
ACCACCTGATTGCCGACGACGGCGTGCGCAGTCACCGGCGCACCCGTGCGGGCGACGCGCATGAAGCCGGCAAGAAGCCC
AGTACGCCGGGAATTGGCTTGGGAATGCCCGCGATTGGGGCCCCGAAGACAGAGCGGATTGAGGTTGCAGGGTGCGGGCT
ATGA

Upstream 100 bases:

>100_bases
GACGATTGGTGGCAGGGCTGGCACGTTCGGGATCGTGCCGGCGGAAGCGGTGCCAGTCGGCGCTGAAATCCACCTGAACG
CGGGCACGTACCATAAGCCA

Downstream 100 bases:

>100_bases
ATGACTCCGAGCCGGGAAAAGAAGACGCCGAATTCGATGAGTTCGCGGCGTTTCACAAAGACATCGACGCCGCCGAGAAG
AAGGTCGCCGGCGAAATCGA

Product: putative tRNA-i(6)A37 modification enzyme MiaB

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MTSTATSGESAVRTYQVRTYGCQMNVHDSERVSGLLDAAGYVKAPEGTDADIVIFNTCAVRENADNKLYGNISHLAPRKA
ANPNMQIAVGGCLAQKDREGMLAKAPWVDVVFGTHNIGSLPALLERARHNNEAQVEIVEALEHFPSALPATRESAYAAWV
SISVGCNNTCTFCIVPSLRGKEIDRRPGDILGEVQALVDQGVLEVTLLGQNVNAYGVNFADPEIPRDRGAFAELLRACGR
IDGLERVRFTSPHPAEFTDDVIEAMAQTPNVCPQLHMPLQSGSDRILKAMRRSYRSERFLSIIDKVRSAMPDAAITTDII
VGFPGETEEDFQQTLEVVRRARFSSAFTFQYSIRPGTPAAKLPDQLPKAVVQERYDRLIALQESVTLEENQKQIGRMIEV
LIATGEGRKDGETARMSGRARDGRLVHFRPQGHVDGALRPGDIITVDVTGAAPHHLIADDGVRSHRRTRAGDAHEAGKKP
STPGIGLGMPAIGAPKTERIEVAGCGL

Sequences:

>Translated_507_residues
MTSTATSGESAVRTYQVRTYGCQMNVHDSERVSGLLDAAGYVKAPEGTDADIVIFNTCAVRENADNKLYGNISHLAPRKA
ANPNMQIAVGGCLAQKDREGMLAKAPWVDVVFGTHNIGSLPALLERARHNNEAQVEIVEALEHFPSALPATRESAYAAWV
SISVGCNNTCTFCIVPSLRGKEIDRRPGDILGEVQALVDQGVLEVTLLGQNVNAYGVNFADPEIPRDRGAFAELLRACGR
IDGLERVRFTSPHPAEFTDDVIEAMAQTPNVCPQLHMPLQSGSDRILKAMRRSYRSERFLSIIDKVRSAMPDAAITTDII
VGFPGETEEDFQQTLEVVRRARFSSAFTFQYSIRPGTPAAKLPDQLPKAVVQERYDRLIALQESVTLEENQKQIGRMIEV
LIATGEGRKDGETARMSGRARDGRLVHFRPQGHVDGALRPGDIITVDVTGAAPHHLIADDGVRSHRRTRAGDAHEAGKKP
STPGIGLGMPAIGAPKTERIEVAGCGL
>Mature_506_residues
TSTATSGESAVRTYQVRTYGCQMNVHDSERVSGLLDAAGYVKAPEGTDADIVIFNTCAVRENADNKLYGNISHLAPRKAA
NPNMQIAVGGCLAQKDREGMLAKAPWVDVVFGTHNIGSLPALLERARHNNEAQVEIVEALEHFPSALPATRESAYAAWVS
ISVGCNNTCTFCIVPSLRGKEIDRRPGDILGEVQALVDQGVLEVTLLGQNVNAYGVNFADPEIPRDRGAFAELLRACGRI
DGLERVRFTSPHPAEFTDDVIEAMAQTPNVCPQLHMPLQSGSDRILKAMRRSYRSERFLSIIDKVRSAMPDAAITTDIIV
GFPGETEEDFQQTLEVVRRARFSSAFTFQYSIRPGTPAAKLPDQLPKAVVQERYDRLIALQESVTLEENQKQIGRMIEVL
IATGEGRKDGETARMSGRARDGRLVHFRPQGHVDGALRPGDIITVDVTGAAPHHLIADDGVRSHRRTRAGDAHEAGKKPS
TPGIGLGMPAIGAPKTERIEVAGCGL

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=475, Percent_Identity=34.9473684210526, Blast_Score=286, Evalue=4e-77,
Organism=Homo sapiens, GI28872782, Length=475, Percent_Identity=34.9473684210526, Blast_Score=285, Evalue=6e-77,
Organism=Homo sapiens, GI93277076, Length=401, Percent_Identity=28.6783042394015, Blast_Score=166, Evalue=4e-41,
Organism=Escherichia coli, GI1786882, Length=450, Percent_Identity=39.3333333333333, Blast_Score=330, Evalue=2e-91,
Organism=Escherichia coli, GI1787057, Length=461, Percent_Identity=33.6225596529284, Blast_Score=220, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI17553146, Length=468, Percent_Identity=34.8290598290598, Blast_Score=250, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI71996771, Length=224, Percent_Identity=35.7142857142857, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI21356207, Length=431, Percent_Identity=36.1948955916473, Blast_Score=281, Evalue=9e-76,
Organism=Drosophila melanogaster, GI19922432, Length=403, Percent_Identity=30.5210918114144, Blast_Score=167, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_MYCA9 (B1MD05)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001703780.1
- ProteinModelPortal:   B1MD05
- SMR:   B1MD05
- EnsemblBacteria:   EBMYCT00000006046
- GeneID:   5965555
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_3048c
- GeneTree:   EBGT00050000015784
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- BioCyc:   MABS561007:MAB_3048C-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 55043; Mature: 54912

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTATSGESAVRTYQVRTYGCQMNVHDSERVSGLLDAAGYVKAPEGTDADIVIFNTCAV
CCCCCCCCCHHHEEEEEEEEEEEECCCCHHHHHHHHHCCCCEECCCCCCCCEEEEEEEEE
RENADNKLYGNISHLAPRKAANPNMQIAVGGCLAQKDREGMLAKAPWVDVVFGTHNIGSL
CCCCCCCEECCHHHHCCCCCCCCCEEEEECCHHHCCCCCCCEEECCCEEEEECCCCCCHH
PALLERARHNNEAQVEIVEALEHFPSALPATRESAYAAWVSISVGCNNTCTFCIVPSLRG
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHCCEEEEEEEEECCCCCEEEEEECCCCC
KEIDRRPGDILGEVQALVDQGVLEVTLLGQNVNAYGVNFADPEIPRDRGAFAELLRACGR
CCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHC
IDGLERVRFTSPHPAEFTDDVIEAMAQTPNVCPQLHMPLQSGSDRILKAMRRSYRSERFL
CCCHHHEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
SIIDKVRSAMPDAAITTDIIVGFPGETEEDFQQTLEVVRRARFSSAFTFQYSIRPGTPAA
HHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHH
KLPDQLPKAVVQERYDRLIALQESVTLEENQKQIGRMIEVLIATGEGRKDGETARMSGRA
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCC
RDGRLVHFRPQGHVDGALRPGDIITVDVTGAAPHHLIADDGVRSHRRTRAGDAHEAGKKP
CCCCEEEECCCCCCCCCCCCCCEEEEEECCCCCCCEECCCCHHHHHHHCCCCHHHCCCCC
STPGIGLGMPAIGAPKTERIEVAGCGL
CCCCCCCCCCCCCCCCCCEEEEECCCC
>Mature Secondary Structure 
TSTATSGESAVRTYQVRTYGCQMNVHDSERVSGLLDAAGYVKAPEGTDADIVIFNTCAV
CCCCCCCCHHHEEEEEEEEEEEECCCCHHHHHHHHHCCCCEECCCCCCCCEEEEEEEEE
RENADNKLYGNISHLAPRKAANPNMQIAVGGCLAQKDREGMLAKAPWVDVVFGTHNIGSL
CCCCCCCEECCHHHHCCCCCCCCCEEEEECCHHHCCCCCCCEEECCCEEEEECCCCCCHH
PALLERARHNNEAQVEIVEALEHFPSALPATRESAYAAWVSISVGCNNTCTFCIVPSLRG
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHCCEEEEEEEEECCCCCEEEEEECCCCC
KEIDRRPGDILGEVQALVDQGVLEVTLLGQNVNAYGVNFADPEIPRDRGAFAELLRACGR
CCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHC
IDGLERVRFTSPHPAEFTDDVIEAMAQTPNVCPQLHMPLQSGSDRILKAMRRSYRSERFL
CCCHHHEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
SIIDKVRSAMPDAAITTDIIVGFPGETEEDFQQTLEVVRRARFSSAFTFQYSIRPGTPAA
HHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHH
KLPDQLPKAVVQERYDRLIALQESVTLEENQKQIGRMIEVLIATGEGRKDGETARMSGRA
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCC
RDGRLVHFRPQGHVDGALRPGDIITVDVTGAAPHHLIADDGVRSHRRTRAGDAHEAGKKP
CCCCEEEECCCCCCCCCCCCCCEEEEEECCCCCCCEECCCCHHHHHHHCCCCHHHCCCCC
STPGIGLGMPAIGAPKTERIEVAGCGL
CCCCCCCCCCCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA