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The map label for this gene is ogt [H]

Identifier: 169630095

GI number: 169630095

Start: 3070132

End: 3070665

Strand: Direct

Name: ogt [H]

Synonym: MAB_3012

Alternate gene names: 169630095

Gene position: 3070132-3070665 (Clockwise)

Preceding gene: 169630094

Following gene: 169630096

Centisome position: 60.59

GC content: 67.79

Gene sequence:

>534_bases
GTGAGCACCGTCGGCCACTGCTTTTTCGATACCGCTATCGGCAGCTGTGCTATCGCGTGGAGCGACAGGGGCATCGTCGC
GTTACAGCTGCCCGAACGCGACGACGCCGCGACCCTTGCCAGGATCCGGCGTCCTGCGGGCAGCGAGCTGGCGCCACCTG
ACTTCGTGGCCCAGGCGATTGACGGAATTCGCCGCGTGCTCGCCGGCGACGACGATGATCTGCAGTGGGCCCGGCTGGAT
CTGGACGGCACCACCGACTTCGATCGCGAGGTCTACGCGGCGACGCGAGCCATCGGTCCGGGCAGGACCCGCAGTTACGG
TGAGGTGGCGGCCTCGGTGGGTGAGCCGGGCGCCGCACAGGCCGTCGGACAGTCTTTGGGACGCAACCCGATTCCGCTCC
TTGTCCCCTGCCACCGGGTGCTTGCCGCCGATCGCAGTCTGCACGGCTTCTCCGCCTACGGCGGCGTAGTGACCAAACGC
GAACTGTTGCGGCTCGAGCACACCCCCGGTTTCGACGACCCCACGCTGTTCTGA

Upstream 100 bases:

>100_bases
ATCGCGGAGCTGCGGCGCGCGCTGGAATGACGGCCGCACCGCACGGCACGGACCCACCGGCGAGTTCGATCTGTCAGTGG
GTCCGGCCATACTGGGCAGC

Downstream 100 bases:

>100_bases
CGCGCAAGCCCCATCACCGGTCTAGGCTCGAGCCCAGCTCTCGCAAGATCACCGGCTTACGCATCGGGAGAACCCATGAC
AGGTCTGTACGTAGCTGCGC

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 177; Mature: 176

Protein sequence:

>177_residues
MSTVGHCFFDTAIGSCAIAWSDRGIVALQLPERDDAATLARIRRPAGSELAPPDFVAQAIDGIRRVLAGDDDDLQWARLD
LDGTTDFDREVYAATRAIGPGRTRSYGEVAASVGEPGAAQAVGQSLGRNPIPLLVPCHRVLAADRSLHGFSAYGGVVTKR
ELLRLEHTPGFDDPTLF

Sequences:

>Translated_177_residues
MSTVGHCFFDTAIGSCAIAWSDRGIVALQLPERDDAATLARIRRPAGSELAPPDFVAQAIDGIRRVLAGDDDDLQWARLD
LDGTTDFDREVYAATRAIGPGRTRSYGEVAASVGEPGAAQAVGQSLGRNPIPLLVPCHRVLAADRSLHGFSAYGGVVTKR
ELLRLEHTPGFDDPTLF
>Mature_176_residues
STVGHCFFDTAIGSCAIAWSDRGIVALQLPERDDAATLARIRRPAGSELAPPDFVAQAIDGIRRVLAGDDDDLQWARLDL
DGTTDFDREVYAATRAIGPGRTRSYGEVAASVGEPGAAQAVGQSLGRNPIPLLVPCHRVLAADRSLHGFSAYGGVVTKRE
LLRLEHTPGFDDPTLF

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=167, Percent_Identity=35.3293413173653, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1787596, Length=84, Percent_Identity=46.4285714285714, Blast_Score=76, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI115533068, Length=85, Percent_Identity=40, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI115533070, Length=85, Percent_Identity=40, Blast_Score=69, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6320001, Length=67, Percent_Identity=40.2985074626866, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18912; Mature: 18781

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVGHCFFDTAIGSCAIAWSDRGIVALQLPERDDAATLARIRRPAGSELAPPDFVAQAI
CCCCHHHHHHHHHCCEEEEECCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCHHHHHHHH
DGIRRVLAGDDDDLQWARLDLDGTTDFDREVYAATRAIGPGRTRSYGEVAASVGEPGAAQ
HHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCHHH
AVGQSLGRNPIPLLVPCHRVLAADRSLHGFSAYGGVVTKRELLRLEHTPGFDDPTLF
HHHHHHCCCCCCEEHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
STVGHCFFDTAIGSCAIAWSDRGIVALQLPERDDAATLARIRRPAGSELAPPDFVAQAI
CCCHHHHHHHHHCCEEEEECCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCHHHHHHHH
DGIRRVLAGDDDDLQWARLDLDGTTDFDREVYAATRAIGPGRTRSYGEVAASVGEPGAAQ
HHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCHHH
AVGQSLGRNPIPLLVPCHRVLAADRSLHGFSAYGGVVTKRELLRLEHTPGFDDPTLF
HHHHHHCCCCCCEEHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]