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The map label for this gene is azoB [H]

Identifier: 169630083

GI number: 169630083

Start: 3061119

End: 3061979

Strand: Direct

Name: azoB [H]

Synonym: MAB_3000

Alternate gene names: 169630083

Gene position: 3061119-3061979 (Clockwise)

Preceding gene: 169630081

Following gene: 169630084

Centisome position: 60.41

GC content: 67.25

Gene sequence:

>861_bases
ATGACAATTCTCGTCACCGGCGCTACAGGCAATGTGGGCCGTCCTCTTGTCGATCTACTGGTCGCGGCGGGGGCCGACGT
CCGCGCGGTCACCCAGCATCCAGAACGCGCGAGCTTTCCACCACAGGTCGCCACCGTCGCGTCGGCCCGTGCTGGGCTGG
ACGGAGTCACGGCAGTTTTCCTGAATTCGCGCGCACTGGGAGAAGATCTCGACAGGTTCGTGAATCAATCCGCGCGGGAA
GGAGTTACCCGCCTCGTCGCGCTCTCCGCCATCAACTGCGACGACGACTTCTCGCGCCAGCCATCCCGGTTCCGTGGCGA
CCGGAACAAAGAAGTAGAGCAGTACGCCGCCGGCTCCGGGCTTCAGTGGGTGAGTCTGCGTCCGACGGTCTTCGCCTCCA
ACTTCCTGGGCATGTGGGGCACGCAGCTCCAGGCAGGCGATGTGGTCCGCGGACCGCACGCGGCGGCGTCGGCAGCCCCG
ATCGCCGACAGCGACATCTCCGAGGTCGCGGCGCGGGCCCTGCTCACCGACGAACTCCTCGGCCAGCGCATCCCGCTGAC
CGGCCCGCAGGCTTTCACCAACGCCGGCCTGGTCGCAGTGCTCGGCGACGTCCTGGGCAGGCCACTGGAATACCAACAGG
TCTCCGACGATCTCGTGCGCCAGCGCTTCGGCCGCCTGGGGTTTCCCGCCGGATTCGCCGACGCGTATCTGGCGATGCAG
GCGGCCACCGTGACCGAGCCCGCCATCGTCACCCATGACGTAGACCGAATACTCGGACGCCCTGCCGAGACGTTCGCGTC
CTGGGCCAACAGATTCCGGACCCGATTCGCCCCCGCAGAGTTCGCGGCGCAGAAGGGATGA

Upstream 100 bases:

>100_bases
GTCCCGCCAGTAACTTTTCGGGCCCGCGCCCCGTCGTATTCGCGTGGGCCAAAGACCCAGCTGGACACCATCTCGAACCT
CCCGAATTGGAGTACCCGTC

Downstream 100 bases:

>100_bases
CGACCGTGACCAACACACAACCACCACGGTGGCTCAAGCCAATGAACAAAGTGGTGCGCGCCTTTCATCGCCTCGGCGTC
TCCACCGGCCCGGCCATGGT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MTILVTGATGNVGRPLVDLLVAAGADVRAVTQHPERASFPPQVATVASARAGLDGVTAVFLNSRALGEDLDRFVNQSARE
GVTRLVALSAINCDDDFSRQPSRFRGDRNKEVEQYAAGSGLQWVSLRPTVFASNFLGMWGTQLQAGDVVRGPHAAASAAP
IADSDISEVAARALLTDELLGQRIPLTGPQAFTNAGLVAVLGDVLGRPLEYQQVSDDLVRQRFGRLGFPAGFADAYLAMQ
AATVTEPAIVTHDVDRILGRPAETFASWANRFRTRFAPAEFAAQKG

Sequences:

>Translated_286_residues
MTILVTGATGNVGRPLVDLLVAAGADVRAVTQHPERASFPPQVATVASARAGLDGVTAVFLNSRALGEDLDRFVNQSARE
GVTRLVALSAINCDDDFSRQPSRFRGDRNKEVEQYAAGSGLQWVSLRPTVFASNFLGMWGTQLQAGDVVRGPHAAASAAP
IADSDISEVAARALLTDELLGQRIPLTGPQAFTNAGLVAVLGDVLGRPLEYQQVSDDLVRQRFGRLGFPAGFADAYLAMQ
AATVTEPAIVTHDVDRILGRPAETFASWANRFRTRFAPAEFAAQKG
>Mature_285_residues
TILVTGATGNVGRPLVDLLVAAGADVRAVTQHPERASFPPQVATVASARAGLDGVTAVFLNSRALGEDLDRFVNQSAREG
VTRLVALSAINCDDDFSRQPSRFRGDRNKEVEQYAAGSGLQWVSLRPTVFASNFLGMWGTQLQAGDVVRGPHAAASAAPI
ADSDISEVAARALLTDELLGQRIPLTGPQAFTNAGLVAVLGDVLGRPLEYQQVSDDLVRQRFGRLGFPAGFADAYLAMQA
ATVTEPAIVTHDVDRILGRPAETFASWANRFRTRFAPAEFAAQKG

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Uses preferentially NADPH rather than NADH as an electron donor for its activity. The enzyme reductively cleaved Orange II and carboxy-Orange II, and can a

COG id: COG0702

COG function: function code MG; Predicted nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NmrA-type oxidoreductase family. Azoreductase type 3 subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR008030 [H]

Pfam domain/function: PF05368 NmrA [H]

EC number: NA

Molecular weight: Translated: 30416; Mature: 30284

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTILVTGATGNVGRPLVDLLVAAGADVRAVTQHPERASFPPQVATVASARAGLDGVTAVF
CEEEEECCCCCCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHH
LNSRALGEDLDRFVNQSAREGVTRLVALSAINCDDDFSRQPSRFRGDRNKEVEQYAAGSG
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCHHHHHHHCCCC
LQWVSLRPTVFASNFLGMWGTQLQAGDVVRGPHAAASAAPIADSDISEVAARALLTDELL
CEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
GQRIPLTGPQAFTNAGLVAVLGDVLGRPLEYQQVSDDLVRQRFGRLGFPAGFADAYLAMQ
CCCCCCCCCHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
AATVTEPAIVTHDVDRILGRPAETFASWANRFRTRFAPAEFAAQKG
HHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHCCCC
>Mature Secondary Structure 
TILVTGATGNVGRPLVDLLVAAGADVRAVTQHPERASFPPQVATVASARAGLDGVTAVF
EEEEECCCCCCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHH
LNSRALGEDLDRFVNQSAREGVTRLVALSAINCDDDFSRQPSRFRGDRNKEVEQYAAGSG
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCHHHHHHHCCCC
LQWVSLRPTVFASNFLGMWGTQLQAGDVVRGPHAAASAAPIADSDISEVAARALLTDELL
CEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
GQRIPLTGPQAFTNAGLVAVLGDVLGRPLEYQQVSDDLVRQRFGRLGFPAGFADAYLAMQ
CCCCCCCCCHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
AATVTEPAIVTHDVDRILGRPAETFASWANRFRTRFAPAEFAAQKG
HHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12147495 [H]