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The map label for this gene is guaD [H]

Identifier: 169630011

GI number: 169630011

Start: 2983312

End: 2984718

Strand: Direct

Name: guaD [H]

Synonym: MAB_2928

Alternate gene names: 169630011

Gene position: 2983312-2984718 (Clockwise)

Preceding gene: 169630004

Following gene: 169630017

Centisome position: 58.88

GC content: 66.38

Gene sequence:

>1407_bases
ATGGAACCGATCGCCGCCACCGTGCACCGCGGACACATCTTCCACATTACGGGCGCTCCCCCGCTGGCCGACGCGGCCAC
GCATCTGCAGTCGATTCGTGACGGCGCCCTGGCAGTCGGAACCGATGGCGTCATCGCTTACTGCGGACCGTACTCCACGC
TGCCCCAGGAATTTACCTCGTGGACCGAGCACCACCACGGGGGGTTCCTGCTGCCCGGGTTCGTCGATACGCATATGCAC
TTCCCGCAGGTGTATTGCACCGACTCCTACGGCGGCGGCCAACTGCTGGAGTGGCTGGAACGCTGCATCTTCCCCGCCGA
GGCCCGCCTGGCCGACACCGCACTGGCGCAGCGCGCGGCGATCGCGTTCTGCCGCCGCCGCATCGCCGCCGGCACCACTG
CCGCGATGGTGATGGGATCGGCCTTTCCGCAGGCGCAGGAGGCCTTGTTCACCGCGACACGTGCCGCCGGACTGCGCATG
GTCAGCGGGCGCGGAGTGCAGACCGTGGGACCCCGCAGTGCCGAGCCGTTGCTCACCACGACCGAACGTGCGACCGAGTT
GTGTGCCAACGAAATCCAGAGCTGGCACGGTGACGAACTCACCGATGTGGCGCTGGTACCGCGGTTCTCGCTGTCGGTCA
CCGCAGAGACCCTCGCGGCCCTCGGCGAGCTCTACGAGGACGTACGCGCGGACGGCGTCTACTTCCACTCCCACCTCAAC
GAGAACGCCCGGCCGGACACCGGTGAGGTGGATGCCACCCTGACGGCGTACGGAGTGGGAAGCTACTTGGATACCTATGA
CGGTCATTTCCTGCCCGGCTCCCGCAAGGGGGGCGCGACCCTATTGGGCCGCCGGAGCATCCTGGCCCACGCTGTGCATT
GCCAGGACGCGGAACTGGCCCGTATGGCCGAAACGGGTAGCTCGATAGCACACTGTCCCACGTCGCAGTTATTCCTGGGT
TCGGGCACCATGCCCTGGCGCCGGACCGTGGCCAGCGGCGTCAATATCGCGCTGGGCACCGATGTGGGCGGCGGTGACGA
ATGGCTGTTGACCCGCGTGTTGAACGACTGCTTCAAGGTCCACATGTCCGAACCGGGCCCGGCCGCGGTGTCCCTGCACC
CGGCCGAACTGCTGTTCACCGCCACACTGGCCGGGGCGCGCGCACTGGACCGTGAGGATACCTTCGGCAACTTGGATGCG
GGCAAGGAGGCAGATTTCCTGGTCGTGGTTCCTGACCGCTGGGAACCGTTGGCCAACAACCTTTTCCATGGCATACGGTC
AGACGATGAGCGCCTGGCCGACGAACAGACACTGTTTCGCCTGCTGATGGGTCTGCGGGAGCCCGCCATCAGCGCCGTAC
TGGTACGTGGCCGCGATATCACCGGCTACCTCCACAGCGACCGCTAA

Upstream 100 bases:

>100_bases
GCAGCGAATACATTCTCGATCAACGTTATTGGATTCATCGCGGGGCCTGTTCGTAACGCATCGTGCTGCGCCCGTGAGAT
GCACTGTGCACTATGGTGCC

Downstream 100 bases:

>100_bases
ACCAACCAGGACAGATGCGGAGACCAGGCCGGCCCCGCATCGGGGGCGTCATCACGGGTAACCGCGGCCTGAATCAACCC
ATACGGACGGTCATCGGCAT

Product: amidohydrolase

Products: NA

Alternate protein names: Guanase; Guanine aminase; Guanine aminohydrolase; GAH [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MEPIAATVHRGHIFHITGAPPLADAATHLQSIRDGALAVGTDGVIAYCGPYSTLPQEFTSWTEHHHGGFLLPGFVDTHMH
FPQVYCTDSYGGGQLLEWLERCIFPAEARLADTALAQRAAIAFCRRRIAAGTTAAMVMGSAFPQAQEALFTATRAAGLRM
VSGRGVQTVGPRSAEPLLTTTERATELCANEIQSWHGDELTDVALVPRFSLSVTAETLAALGELYEDVRADGVYFHSHLN
ENARPDTGEVDATLTAYGVGSYLDTYDGHFLPGSRKGGATLLGRRSILAHAVHCQDAELARMAETGSSIAHCPTSQLFLG
SGTMPWRRTVASGVNIALGTDVGGGDEWLLTRVLNDCFKVHMSEPGPAAVSLHPAELLFTATLAGARALDREDTFGNLDA
GKEADFLVVVPDRWEPLANNLFHGIRSDDERLADEQTLFRLLMGLREPAISAVLVRGRDITGYLHSDR

Sequences:

>Translated_468_residues
MEPIAATVHRGHIFHITGAPPLADAATHLQSIRDGALAVGTDGVIAYCGPYSTLPQEFTSWTEHHHGGFLLPGFVDTHMH
FPQVYCTDSYGGGQLLEWLERCIFPAEARLADTALAQRAAIAFCRRRIAAGTTAAMVMGSAFPQAQEALFTATRAAGLRM
VSGRGVQTVGPRSAEPLLTTTERATELCANEIQSWHGDELTDVALVPRFSLSVTAETLAALGELYEDVRADGVYFHSHLN
ENARPDTGEVDATLTAYGVGSYLDTYDGHFLPGSRKGGATLLGRRSILAHAVHCQDAELARMAETGSSIAHCPTSQLFLG
SGTMPWRRTVASGVNIALGTDVGGGDEWLLTRVLNDCFKVHMSEPGPAAVSLHPAELLFTATLAGARALDREDTFGNLDA
GKEADFLVVVPDRWEPLANNLFHGIRSDDERLADEQTLFRLLMGLREPAISAVLVRGRDITGYLHSDR
>Mature_468_residues
MEPIAATVHRGHIFHITGAPPLADAATHLQSIRDGALAVGTDGVIAYCGPYSTLPQEFTSWTEHHHGGFLLPGFVDTHMH
FPQVYCTDSYGGGQLLEWLERCIFPAEARLADTALAQRAAIAFCRRRIAAGTTAAMVMGSAFPQAQEALFTATRAAGLRM
VSGRGVQTVGPRSAEPLLTTTERATELCANEIQSWHGDELTDVALVPRFSLSVTAETLAALGELYEDVRADGVYFHSHLN
ENARPDTGEVDATLTAYGVGSYLDTYDGHFLPGSRKGGATLLGRRSILAHAVHCQDAELARMAETGSSIAHCPTSQLFLG
SGTMPWRRTVASGVNIALGTDVGGGDEWLLTRVLNDCFKVHMSEPGPAAVSLHPAELLFTATLAGARALDREDTFGNLDA
GKEADFLVVVPDRWEPLANNLFHGIRSDDERLADEQTLFRLLMGLREPAISAVLVRGRDITGYLHSDR

Specific function: Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia [H]

COG id: COG0402

COG function: function code FR; Cytosine deaminase and related metal-dependent hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATZ/TRZ family [H]

Homologues:

Organism=Homo sapiens, GI4758426, Length=437, Percent_Identity=29.0617848970252, Blast_Score=146, Evalue=3e-35,
Organism=Escherichia coli, GI1789249, Length=391, Percent_Identity=32.7365728900256, Blast_Score=205, Evalue=5e-54,
Organism=Saccharomyces cerevisiae, GI6319963, Length=363, Percent_Identity=28.6501377410468, Blast_Score=142, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24643849, Length=429, Percent_Identity=29.8368298368298, Blast_Score=135, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR014311 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.4.3 [H]

Molecular weight: Translated: 50397; Mature: 50397

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPIAATVHRGHIFHITGAPPLADAATHLQSIRDGALAVGTDGVIAYCGPYSTLPQEFTS
CCCHHHEEECCEEEEECCCCCHHHHHHHHHHHHCCCEEECCCCEEEECCCCCCCHHHHHH
WTEHHHGGFLLPGFVDTHMHFPQVYCTDSYGGGQLLEWLERCIFPAEARLADTALAQRAA
HHHHCCCCEECCCHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
IAFCRRRIAAGTTAAMVMGSAFPQAQEALFTATRAAGLRMVSGRGVQTVGPRSAEPLLTT
HHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCEEC
TERATELCANEIQSWHGDELTDVALVPRFSLSVTAETLAALGELYEDVRADGVYFHSHLN
HHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
ENARPDTGEVDATLTAYGVGSYLDTYDGHFLPGSRKGGATLLGRRSILAHAVHCQDAELA
CCCCCCCCCCCEEEEEEECCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHH
RMAETGSSIAHCPTSQLFLGSGTMPWRRTVASGVNIALGTDVGGGDEWLLTRVLNDCFKV
HHHHCCCCCCCCCCCCEEECCCCCHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHH
HMSEPGPAAVSLHPAELLFTATLAGARALDREDTFGNLDAGKEADFLVVVPDRWEPLANN
HCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHH
LFHGIRSDDERLADEQTLFRLLMGLREPAISAVLVRGRDITGYLHSDR
HHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHEEEEECCCEEEEECCCC
>Mature Secondary Structure
MEPIAATVHRGHIFHITGAPPLADAATHLQSIRDGALAVGTDGVIAYCGPYSTLPQEFTS
CCCHHHEEECCEEEEECCCCCHHHHHHHHHHHHCCCEEECCCCEEEECCCCCCCHHHHHH
WTEHHHGGFLLPGFVDTHMHFPQVYCTDSYGGGQLLEWLERCIFPAEARLADTALAQRAA
HHHHCCCCEECCCHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
IAFCRRRIAAGTTAAMVMGSAFPQAQEALFTATRAAGLRMVSGRGVQTVGPRSAEPLLTT
HHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCEEC
TERATELCANEIQSWHGDELTDVALVPRFSLSVTAETLAALGELYEDVRADGVYFHSHLN
HHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
ENARPDTGEVDATLTAYGVGSYLDTYDGHFLPGSRKGGATLLGRRSILAHAVHCQDAELA
CCCCCCCCCCCEEEEEEECCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHH
RMAETGSSIAHCPTSQLFLGSGTMPWRRTVASGVNIALGTDVGGGDEWLLTRVLNDCFKV
HHHHCCCCCCCCCCCCEEECCCCCHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHH
HMSEPGPAAVSLHPAELLFTATLAGARALDREDTFGNLDAGKEADFLVVVPDRWEPLANN
HCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHH
LFHGIRSDDERLADEQTLFRLLMGLREPAISAVLVRGRDITGYLHSDR
HHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHEEEEECCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]