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The map label for this gene is ansP2 [H]

Identifier: 169629997

GI number: 169629997

Start: 2963131

End: 2964615

Strand: Direct

Name: ansP2 [H]

Synonym: MAB_2914

Alternate gene names: 169629997

Gene position: 2963131-2964615 (Clockwise)

Preceding gene: 169629996

Following gene: 169629999

Centisome position: 58.48

GC content: 62.49

Gene sequence:

>1485_bases
ATGACATCTCCGGCAACTTATGACACGTCGGCCCCCGCCCCGGAGGAGCAGGGCTATCACAAGGGACTGAATAACCGGCA
AGTCCAAATGATCGGCATCGGGGGCGCCATCGGCACGGGGCTGTTCATGGGAGCCGGCGGCCGGTTGCACAGTGCGGGAC
CGGGCTTGTTCTTGGTTTATGCGGTCTGCGGCGTGTTCGTGTTCTTCATCCTGCGTGCGTTGGGCGAGCTGATCTTGCAC
CGGCCGTCCTCGGGATCCTTCGTGTCGTACGCCCGCGAATTCCTCGGCGAGAAGGCAGCGTACGTCGCGGGCTGGATGTA
CTTCTTCAACTGGGCCGCCACCGCCATCGTCGACGTGACCGCCATCGCGCTGTACATGCACTACTGGAGCGCCTTCAAGG
CCATCCCTCAGTGGTCCATCGCACTCATTGCCTTGGTCATCGTGCTGACCATGAACACCATCAGCGTCAAACTGTTCGGG
GAGATGGAATTCTGGGCGGCTCTGATCAAAGTGGTTGCCATCATGGGGTTCCTGGTCATCGGGACGGTCTTCCTGGCGGG
CCGGTTCACGGTCGAGGGCGCCAGCACAGGACCATCGGTGATCGCGGACAACGGCGGCCTGCTGCCCACCGGCCTGATGG
CATTGGTCATCGTCACCTCGGGAGTCGTCTTCGCGTACTCCGCAGTCGAACTGGTCGGCATCGCCGCCGGCGAGACCGAA
AACCCTGACAAGGTGATGCCCCGGGCCATCAACTCCGTCGTGTTCCGGGTCGCGGTCTTCTACGTGGGTTCGCTCATCTT
GCTGGCACTGCTGCTGCCCTATGGGACCTACAAAGCCGGCGAAAGCCCCTTCGTCACCTTCTTCTCCCGGATAGGCGTGC
CGTACGCCGGCGATGTCATGAACTTTGTGGTGCTGACAGCCGCACTGTCCAGCCTGAACGCGGGCCTGTACAGCACGGGG
CGGATTCTGCGTTCACTGGCGATGAACGGTAGTGCACCAAAAGTGTTGTCACGCATGACCTCCGGCGGAGTGCCCTTCAT
GGGCATCGCGGTCACCGGTGCCCTCACACTTGTCGGTATATTCATGAACCTGGTCATTCCCGCCGAAGCCTTCGAAACCG
CTCTGGATCTGGCGGCACTCGGGATCATCTCGTCATGGGCCACCATCGTGATCTGCCAGATCCAGCTGTACCGGTGGTCA
CAGCGAGGCATCCTGCAACGGGGCAGTTTCCGGATGCCCGGCGCCCCGTACACCGGGTACCTGACGCTGGCGTTCCTGGC
GGCCGTGGTGGTGCTGATGTGCTACGAGAACGCGTGGAACCTCATCGCAATAGCGGTACTTGTCCCCGTATTAACCGCGG
GCTGGTACCTGTGCCGCGGTCGCGTGCTGCAGCTCGCCCGCGAGCGGATCGGATACACCGGCGCATACCCGGTGATTGCG
CACACCCCCATGCTCGACGACCCGCCAGGCGAATCGCATAGGTAG

Upstream 100 bases:

>100_bases
GTCTCTTCGTTGCCTTTGGCCGGTCATCCAGCAGAAATACGCAGCATGTTCGTAACATCGTTGTTCCAATCAGCAATTTT
CCGGTCGATACGGTCGCACG

Downstream 100 bases:

>100_bases
GGATCGCCGTTCGGGCACACCCTCGGCCTCTGGTCGAGGGTGTGCGGTTCTCGGATGCCCGCGGCCTGTTTCTCGGGCGA
GGCGTGTCATGCAATCCTCT

Product: L-asparagine permease 1 (L-asparagine transport)

Products: Proton [Cytoplasm]; L-asparagine [Cytoplasm] [C]

Alternate protein names: L-asparagine transport protein 2 [H]

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MTSPATYDTSAPAPEEQGYHKGLNNRQVQMIGIGGAIGTGLFMGAGGRLHSAGPGLFLVYAVCGVFVFFILRALGELILH
RPSSGSFVSYAREFLGEKAAYVAGWMYFFNWAATAIVDVTAIALYMHYWSAFKAIPQWSIALIALVIVLTMNTISVKLFG
EMEFWAALIKVVAIMGFLVIGTVFLAGRFTVEGASTGPSVIADNGGLLPTGLMALVIVTSGVVFAYSAVELVGIAAGETE
NPDKVMPRAINSVVFRVAVFYVGSLILLALLLPYGTYKAGESPFVTFFSRIGVPYAGDVMNFVVLTAALSSLNAGLYSTG
RILRSLAMNGSAPKVLSRMTSGGVPFMGIAVTGALTLVGIFMNLVIPAEAFETALDLAALGIISSWATIVICQIQLYRWS
QRGILQRGSFRMPGAPYTGYLTLAFLAAVVVLMCYENAWNLIAIAVLVPVLTAGWYLCRGRVLQLARERIGYTGAYPVIA
HTPMLDDPPGESHR

Sequences:

>Translated_494_residues
MTSPATYDTSAPAPEEQGYHKGLNNRQVQMIGIGGAIGTGLFMGAGGRLHSAGPGLFLVYAVCGVFVFFILRALGELILH
RPSSGSFVSYAREFLGEKAAYVAGWMYFFNWAATAIVDVTAIALYMHYWSAFKAIPQWSIALIALVIVLTMNTISVKLFG
EMEFWAALIKVVAIMGFLVIGTVFLAGRFTVEGASTGPSVIADNGGLLPTGLMALVIVTSGVVFAYSAVELVGIAAGETE
NPDKVMPRAINSVVFRVAVFYVGSLILLALLLPYGTYKAGESPFVTFFSRIGVPYAGDVMNFVVLTAALSSLNAGLYSTG
RILRSLAMNGSAPKVLSRMTSGGVPFMGIAVTGALTLVGIFMNLVIPAEAFETALDLAALGIISSWATIVICQIQLYRWS
QRGILQRGSFRMPGAPYTGYLTLAFLAAVVVLMCYENAWNLIAIAVLVPVLTAGWYLCRGRVLQLARERIGYTGAYPVIA
HTPMLDDPPGESHR
>Mature_493_residues
TSPATYDTSAPAPEEQGYHKGLNNRQVQMIGIGGAIGTGLFMGAGGRLHSAGPGLFLVYAVCGVFVFFILRALGELILHR
PSSGSFVSYAREFLGEKAAYVAGWMYFFNWAATAIVDVTAIALYMHYWSAFKAIPQWSIALIALVIVLTMNTISVKLFGE
MEFWAALIKVVAIMGFLVIGTVFLAGRFTVEGASTGPSVIADNGGLLPTGLMALVIVTSGVVFAYSAVELVGIAAGETEN
PDKVMPRAINSVVFRVAVFYVGSLILLALLLPYGTYKAGESPFVTFFSRIGVPYAGDVMNFVVLTAALSSLNAGLYSTGR
ILRSLAMNGSAPKVLSRMTSGGVPFMGIAVTGALTLVGIFMNLVIPAEAFETALDLAALGIISSWATIVICQIQLYRWSQ
RGILQRGSFRMPGAPYTGYLTLAFLAAVVVLMCYENAWNLIAIAVLVPVLTAGWYLCRGRVLQLARERIGYTGAYPVIAH
TPMLDDPPGESHR

Specific function: Unknown

COG id: COG1113

COG function: function code E; Gamma-aminobutyrate permease and related permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid transporter (AAT) (TC 2.A.3.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081915, Length=454, Percent_Identity=58.8105726872247, Blast_Score=534, Evalue=1e-153,
Organism=Escherichia coli, GI1790653, Length=428, Percent_Identity=39.4859813084112, Blast_Score=311, Evalue=5e-86,
Organism=Escherichia coli, GI1786789, Length=454, Percent_Identity=36.1233480176211, Blast_Score=302, Evalue=3e-83,
Organism=Escherichia coli, GI1786302, Length=416, Percent_Identity=37.5, Blast_Score=291, Evalue=8e-80,
Organism=Escherichia coli, GI48994972, Length=426, Percent_Identity=37.793427230047, Blast_Score=282, Evalue=4e-77,
Organism=Escherichia coli, GI1786602, Length=457, Percent_Identity=35.4485776805252, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI87081708, Length=403, Percent_Identity=33.7468982630273, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI1788480, Length=347, Percent_Identity=37.463976945245, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1789017, Length=438, Percent_Identity=31.7351598173516, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI2367353, Length=375, Percent_Identity=24.8, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324061, Length=448, Percent_Identity=32.8125, Blast_Score=231, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6320772, Length=434, Percent_Identity=32.7188940092166, Blast_Score=211, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6322892, Length=418, Percent_Identity=31.5789473684211, Blast_Score=182, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6324059, Length=455, Percent_Identity=30.1098901098901, Blast_Score=180, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6324990, Length=447, Percent_Identity=29.3064876957494, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6324924, Length=493, Percent_Identity=26.369168356998, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6321053, Length=457, Percent_Identity=27.3522975929978, Blast_Score=163, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6319608, Length=448, Percent_Identity=25.6696428571429, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6320717, Length=416, Percent_Identity=26.4423076923077, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6319824, Length=399, Percent_Identity=25.8145363408521, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6322967, Length=392, Percent_Identity=27.0408163265306, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6321629, Length=426, Percent_Identity=26.9953051643192, Blast_Score=143, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6324553, Length=403, Percent_Identity=27.5434243176179, Blast_Score=141, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6319543, Length=450, Percent_Identity=25.7777777777778, Blast_Score=136, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6324981, Length=412, Percent_Identity=27.9126213592233, Blast_Score=133, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6320251, Length=406, Percent_Identity=24.6305418719212, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6319542, Length=391, Percent_Identity=25.0639386189258, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6320364, Length=508, Percent_Identity=21.4566929133858, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004840 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 52771; Mature: 52640

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS00218 AMINO_ACID_PERMEASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPATYDTSAPAPEEQGYHKGLNNRQVQMIGIGGAIGTGLFMGAGGRLHSAGPGLFLVY
CCCCCCCCCCCCCCCCCHHHCCCCCCEEEEEEECCHHHHHHHCCCCCCCCCCCCHHHHHH
AVCGVFVFFILRALGELILHRPSSGSFVSYAREFLGEKAAYVAGWMYFFNWAATAIVDVT
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
AIALYMHYWSAFKAIPQWSIALIALVIVLTMNTISVKLFGEMEFWAALIKVVAIMGFLVI
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHH
GTVFLAGRFTVEGASTGPSVIADNGGLLPTGLMALVIVTSGVVFAYSAVELVGIAAGETE
HHHHHHCCEEECCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCC
NPDKVMPRAINSVVFRVAVFYVGSLILLALLLPYGTYKAGESPFVTFFSRIGVPYAGDVM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCHHHH
NFVVLTAALSSLNAGLYSTGRILRSLAMNGSAPKVLSRMTSGGVPFMGIAVTGALTLVGI
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHH
FMNLVIPAEAFETALDLAALGIISSWATIVICQIQLYRWSQRGILQRGSFRMPGAPYTGY
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCHHH
LTLAFLAAVVVLMCYENAWNLIAIAVLVPVLTAGWYLCRGRVLQLARERIGYTGAYPVIA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHE
HTPMLDDPPGESHR
ECCCCCCCCCCCCC
>Mature Secondary Structure 
TSPATYDTSAPAPEEQGYHKGLNNRQVQMIGIGGAIGTGLFMGAGGRLHSAGPGLFLVY
CCCCCCCCCCCCCCCCHHHCCCCCCEEEEEEECCHHHHHHHCCCCCCCCCCCCHHHHHH
AVCGVFVFFILRALGELILHRPSSGSFVSYAREFLGEKAAYVAGWMYFFNWAATAIVDVT
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
AIALYMHYWSAFKAIPQWSIALIALVIVLTMNTISVKLFGEMEFWAALIKVVAIMGFLVI
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHH
GTVFLAGRFTVEGASTGPSVIADNGGLLPTGLMALVIVTSGVVFAYSAVELVGIAAGETE
HHHHHHCCEEECCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCC
NPDKVMPRAINSVVFRVAVFYVGSLILLALLLPYGTYKAGESPFVTFFSRIGVPYAGDVM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCHHHH
NFVVLTAALSSLNAGLYSTGRILRSLAMNGSAPKVLSRMTSGGVPFMGIAVTGALTLVGI
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHH
FMNLVIPAEAFETALDLAALGIISSWATIVICQIQLYRWSQRGILQRGSFRMPGAPYTGY
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCHHH
LTLAFLAAVVVLMCYENAWNLIAIAVLVPVLTAGWYLCRGRVLQLARERIGYTGAYPVIA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHE
HTPMLDDPPGESHR
ECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-asparagine [Periplasm]; Proton [Periplasm] [C]

Specific reaction: L-asparagine [Periplasm] + Proton [Periplasm] = Proton [Cytoplasm] + L-asparagine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]