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The map label for this gene is pdxT

Identifier: 169629974

GI number: 169629974

Start: 2942612

End: 2943193

Strand: Reverse

Name: pdxT

Synonym: MAB_2890c

Alternate gene names: 169629974

Gene position: 2943193-2942612 (Counterclockwise)

Preceding gene: 169629975

Following gene: 169629972

Centisome position: 58.08

GC content: 66.15

Gene sequence:

>582_bases
GTGAGTCCCCTTGTGGGTGTGCTGGCATTGCAGGGCGATGTCAGGGAACATGTTGCGGCGCTGAAAGATTCGGGGGCCGA
GGCGCTTGGTGTGCGTCGCCCCGAGGAGCTTGGCAAGGTCGATGGGCTGGTCATTCCCGGTGGGGAATCGACCACGATGA
GCAACTTGCTGCGCGTGTTCGAGCTGTTGGATCCGCTCACCGAACGGTTGCGTGCCGGGCTGCCGGTTTACGGCTCGTGC
GCCGGCATGATCCTGCTGGCCAGCGAGATCCTGGATACCCGCCCGGACGCGGTCGCACTCGGCGCCATCGATATGACCGT
GCGCCGCAACGCATTCGGACGTCAGGTCGATTCCTTCGAAGGTGACCTGGATTTCACCGGCGTGGACGGTGCGATGCACG
CGGTGTTCATCCGCGCACCGTGGGTGGAGCGTGTCGGAGACGATGTCGAGGTGCTGGCCAGTGCGCAGGGCCACCCGGTC
GCGGTGCGGCAGGGGAGTGCGCTGGCGACGGCATTTCACCCCGAGGTGACCGGCGACCGCCGGGTGCATCAGCTGTTCGT
GGATATGGTCCGCGCCGGCTAG

Upstream 100 bases:

>100_bases
TCAACCGGGACGGAACCATGGTGGCTGCGGTGGTGCAAGAGGGGCTTACCCGCATTGAGCGTAACCCCGAGCAGGCGTCG
GTGGCCCGAGGGAATATGGC

Downstream 100 bases:

>100_bases
CCCTGATGGCGGGTGCCCGGCTATGCGCGCCAAAGCGGGCTGCCGGCTTCAGGGGTGGTGATGTGTCGCGTACAGCTGGA
GTCACCAGCGCAACATGGGC

Product: glutamine amidotransferase subunit PdxT

Products: NA

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MSPLVGVLALQGDVREHVAALKDSGAEALGVRRPEELGKVDGLVIPGGESTTMSNLLRVFELLDPLTERLRAGLPVYGSC
AGMILLASEILDTRPDAVALGAIDMTVRRNAFGRQVDSFEGDLDFTGVDGAMHAVFIRAPWVERVGDDVEVLASAQGHPV
AVRQGSALATAFHPEVTGDRRVHQLFVDMVRAG

Sequences:

>Translated_193_residues
MSPLVGVLALQGDVREHVAALKDSGAEALGVRRPEELGKVDGLVIPGGESTTMSNLLRVFELLDPLTERLRAGLPVYGSC
AGMILLASEILDTRPDAVALGAIDMTVRRNAFGRQVDSFEGDLDFTGVDGAMHAVFIRAPWVERVGDDVEVLASAQGHPV
AVRQGSALATAFHPEVTGDRRVHQLFVDMVRAG
>Mature_192_residues
SPLVGVLALQGDVREHVAALKDSGAEALGVRRPEELGKVDGLVIPGGESTTMSNLLRVFELLDPLTERLRAGLPVYGSCA
GMILLASEILDTRPDAVALGAIDMTVRRNAFGRQVDSFEGDLDFTGVDGAMHAVFIRAPWVERVGDDVEVLASAQGHPVA
VRQGSALATAFHPEVTGDRRVHQLFVDMVRAG

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family

Homologues:

Organism=Saccharomyces cerevisiae, GI6323742, Length=206, Percent_Identity=38.3495145631068, Blast_Score=116, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6323995, Length=212, Percent_Identity=34.4339622641509, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321048, Length=212, Percent_Identity=33.9622641509434, Blast_Score=98, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXT_MYCA9 (B1MCJ8)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001703623.1
- ProteinModelPortal:   B1MCJ8
- SMR:   B1MCJ8
- EnsemblBacteria:   EBMYCT00000005935
- GeneID:   5965398
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_2890c
- GeneTree:   EBGT00050000016176
- HOGENOM:   HBG292341
- OMA:   QGDVREH
- ProtClustDB:   PRK13525
- BioCyc:   MABS561007:MAB_2890C-MONOMER
- HAMAP:   MF_01615
- InterPro:   IPR002161
- InterPro:   IPR021196
- PIRSF:   PIRSF005639
- TIGRFAMs:   TIGR03800

Pfam domain/function: PF01174 SNO

EC number: NA

Molecular weight: Translated: 20537; Mature: 20406

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS01236 PDXT_SNO_1; PS51130 PDXT_SNO_2

Important sites: ACT_SITE 80-80 ACT_SITE 173-173 ACT_SITE 175-175 BINDING 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPLVGVLALQGDVREHVAALKDSGAEALGVRRPEELGKVDGLVIPGGESTTMSNLLRVF
CCCHHHHHHHCCHHHHHHHHHHCCCCHHHCCCCHHHHHCCCCEEECCCCCHHHHHHHHHH
ELLDPLTERLRAGLPVYGSCAGMILLASEILDTRPDAVALGAIDMTVRRNAFGRQVDSFE
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHCCHHCCC
GDLDFTGVDGAMHAVFIRAPWVERVGDDVEVLASAQGHPVAVRQGSALATAFHPEVTGDR
CCCCCCCCCCHHEEEEEECCHHHHHCCCHHHHHCCCCCEEEEECCCEEEEEECCCCCCCH
RVHQLFVDMVRAG
HHHHHHHHHHHCC
>Mature Secondary Structure 
SPLVGVLALQGDVREHVAALKDSGAEALGVRRPEELGKVDGLVIPGGESTTMSNLLRVF
CCHHHHHHHCCHHHHHHHHHHCCCCHHHCCCCHHHHHCCCCEEECCCCCHHHHHHHHHH
ELLDPLTERLRAGLPVYGSCAGMILLASEILDTRPDAVALGAIDMTVRRNAFGRQVDSFE
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHCCHHCCC
GDLDFTGVDGAMHAVFIRAPWVERVGDDVEVLASAQGHPVAVRQGSALATAFHPEVTGDR
CCCCCCCCCCHHEEEEEECCHHHHHCCCHHHHHCCCCCEEEEECCCEEEEEECCCCCCCH
RVHQLFVDMVRAG
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA