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The map label for this gene is aspS

Identifier: 169629949

GI number: 169629949

Start: 2918006

End: 2919799

Strand: Reverse

Name: aspS

Synonym: MAB_2865c

Alternate gene names: 169629949

Gene position: 2919799-2918006 (Counterclockwise)

Preceding gene: 169629950

Following gene: 169629948

Centisome position: 57.62

GC content: 66.16

Gene sequence:

>1794_bases
GTGCTGCGCACTCATGACGCCGGTTCGTTGCGGGAATCGAACGCCGGGCAGCGAGTGACGCTTGCCGGCTGGGTGGCGCG
CCGACGCGATCACGGCGGCGTCATCTTCATCGATCTGCGGGACGCGTCGGGTGTCGCCCAGGTCGTGTTCCGCGAGGACG
CGGTGCTGGAGCAGGCTCACCGGTTGCGCGCGGAATTCTGCGTCGAGGTCAGCGGTGTTGTGGAGGTCCGGCCCGAAGGT
AACGCCAACGACGAGATCGCGACCGGGCAGATCGAAGTCAACGCCGCCGAGCTGAAGGTGCTCAACGAGAGCGCGCCGCT
GCCATTCCAGCTCGATGAGACCGCGGGTGAGGAGGCGCGATTGCGCTACCGCTACCTGGATCTGCGTCGTGAGGGGCCGG
GCAACGCAATCAGATTGCGTTCCAAGGCGAACGCCGCCGCACGCAGCGTGCTGTCCGGTCACGAATTTGTCGAGGTGGAG
ACCCCGACGCTGACTCGTTCGACTCCCGAGGGTGCGCGCGATTTCCTGGTGCCTGCCCGTCTGCAGCCGGGTAGTTTCTA
CGCGCTGCCGCAGAGCCCGCAGCTGTTCAAGCAGCTGCTGATGGTCGCCGGTATGGAGCGGTACTACCAGATCGCCCGCT
GCTACCGCGACGAGGATTTCCGCGCCGACCGGCAACCCGAATTCACCCAGCTCGACATCGAGATGAGCTTCGTGGACCAG
GACGATGTCATCGCGCTCGCCGAGGAGATCCTCGTCGCGCTGTGGGATCTGGTCGGCTACCGGGTGCCTACTCCCATCGA
CCGCATCACCTATGCCGAGGCGATGCGGCGGTACGGCAGCGACAAGCCGGACCTGCGGTTCGGCCTGGAGCTGGTGGAGT
GCACCGACTACTTCTCTGAGACCAGCTTCCGCGTGTTCCAGGCGCCATACGTGGGCGCCGTGGTGATGCCCGGCGGCGCC
GACCAGCCGCGCCGCACCCTGGACGGCTGGCAGGAGTTCGCCAAACAGCGCGGACACAAGGGACTGGCCTACGTCCTGGT
GGGCGAGGACGGCACCCTCGGCGGGCCCGTGGCCAAGAATCTTTCCGATGCCGAACGCGACGGACTGGCGGCTCATGTGG
GCGCGAACCCAGGAGACTGCATTTTCTTCTCCGCCGGCGCCGCCAAGTCATCACGCGCGTTATTGGGTTCGGTGCGTGGC
GAGGTGGCACAGCGCCTCGGGTTGATCGACCCAGACGCCTGGGCCTTCACCTGGGTGGTGGACGCTCCACTGTTCGAGCC
TGCCGATGATGCGACAGCTGCCGGTGATGTGGCGGTCGGTTCGGGCGCGTGGACGGCGGTACACCACGCCTTCACTTCGC
CGAAACCTGAGTCGGAGAACACCTTTGACACCGATCCGGGATCGGCGCTGGCTTACGCTTACGACATCGTGTGCAACGGG
CATGAGATCGGCGGCGGCTCGATCCGTATTCACCGCCGCGACATTCAGGAACGCGTCTTCCAGGTGATGGGTATCAGCAA
CGAGGATGCCCAGGAGAAGTTCGGATTCCTGCTGGACGCGTTCGCCTTCGGTGCACCCCCGCACGGCGGCATCGCCTTCG
GGTGGGATCGCGTGACCGCACTGCTGGCCGGGACATCCTCGATCCGCGAAGTCATTGCCTTCCCGAAGTCCGGTGGTGGT
GTTGACCCGCTGACCGACGCGCCCGCGCCGATCACCGCGGCACAGCGCAAGGAGTCCGGGATTGACGCCAAACCCGAGAA
GGCCGAGAAGGCGGGTAAGCCGGCGGACGCGTAG

Upstream 100 bases:

>100_bases
GTCGGTAAGGCGCCTCGACGGGATGGGGAACCCGGGCCGCTAGGCTCAAACGTCTTAACACTGATTTTCTAATGAACAAG
CACTGTCGAAGGGATCTTTC

Downstream 100 bases:

>100_bases
CCAGTGCTGCATCTCAGGGTGATCGCACCCGCCGAGGTTTCGGATCAGGTGGTCGAATTCCTTTCCACCACCACGGGAGT
CACGCATATCGTGCGCGTGC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 597; Mature: 597

Protein sequence:

>597_residues
MLRTHDAGSLRESNAGQRVTLAGWVARRRDHGGVIFIDLRDASGVAQVVFREDAVLEQAHRLRAEFCVEVSGVVEVRPEG
NANDEIATGQIEVNAAELKVLNESAPLPFQLDETAGEEARLRYRYLDLRREGPGNAIRLRSKANAAARSVLSGHEFVEVE
TPTLTRSTPEGARDFLVPARLQPGSFYALPQSPQLFKQLLMVAGMERYYQIARCYRDEDFRADRQPEFTQLDIEMSFVDQ
DDVIALAEEILVALWDLVGYRVPTPIDRITYAEAMRRYGSDKPDLRFGLELVECTDYFSETSFRVFQAPYVGAVVMPGGA
DQPRRTLDGWQEFAKQRGHKGLAYVLVGEDGTLGGPVAKNLSDAERDGLAAHVGANPGDCIFFSAGAAKSSRALLGSVRG
EVAQRLGLIDPDAWAFTWVVDAPLFEPADDATAAGDVAVGSGAWTAVHHAFTSPKPESENTFDTDPGSALAYAYDIVCNG
HEIGGGSIRIHRRDIQERVFQVMGISNEDAQEKFGFLLDAFAFGAPPHGGIAFGWDRVTALLAGTSSIREVIAFPKSGGG
VDPLTDAPAPITAAQRKESGIDAKPEKAEKAGKPADA

Sequences:

>Translated_597_residues
MLRTHDAGSLRESNAGQRVTLAGWVARRRDHGGVIFIDLRDASGVAQVVFREDAVLEQAHRLRAEFCVEVSGVVEVRPEG
NANDEIATGQIEVNAAELKVLNESAPLPFQLDETAGEEARLRYRYLDLRREGPGNAIRLRSKANAAARSVLSGHEFVEVE
TPTLTRSTPEGARDFLVPARLQPGSFYALPQSPQLFKQLLMVAGMERYYQIARCYRDEDFRADRQPEFTQLDIEMSFVDQ
DDVIALAEEILVALWDLVGYRVPTPIDRITYAEAMRRYGSDKPDLRFGLELVECTDYFSETSFRVFQAPYVGAVVMPGGA
DQPRRTLDGWQEFAKQRGHKGLAYVLVGEDGTLGGPVAKNLSDAERDGLAAHVGANPGDCIFFSAGAAKSSRALLGSVRG
EVAQRLGLIDPDAWAFTWVVDAPLFEPADDATAAGDVAVGSGAWTAVHHAFTSPKPESENTFDTDPGSALAYAYDIVCNG
HEIGGGSIRIHRRDIQERVFQVMGISNEDAQEKFGFLLDAFAFGAPPHGGIAFGWDRVTALLAGTSSIREVIAFPKSGGG
VDPLTDAPAPITAAQRKESGIDAKPEKAEKAGKPADA
>Mature_597_residues
MLRTHDAGSLRESNAGQRVTLAGWVARRRDHGGVIFIDLRDASGVAQVVFREDAVLEQAHRLRAEFCVEVSGVVEVRPEG
NANDEIATGQIEVNAAELKVLNESAPLPFQLDETAGEEARLRYRYLDLRREGPGNAIRLRSKANAAARSVLSGHEFVEVE
TPTLTRSTPEGARDFLVPARLQPGSFYALPQSPQLFKQLLMVAGMERYYQIARCYRDEDFRADRQPEFTQLDIEMSFVDQ
DDVIALAEEILVALWDLVGYRVPTPIDRITYAEAMRRYGSDKPDLRFGLELVECTDYFSETSFRVFQAPYVGAVVMPGGA
DQPRRTLDGWQEFAKQRGHKGLAYVLVGEDGTLGGPVAKNLSDAERDGLAAHVGANPGDCIFFSAGAAKSSRALLGSVRG
EVAQRLGLIDPDAWAFTWVVDAPLFEPADDATAAGDVAVGSGAWTAVHHAFTSPKPESENTFDTDPGSALAYAYDIVCNG
HEIGGGSIRIHRRDIQERVFQVMGISNEDAQEKFGFLLDAFAFGAPPHGGIAFGWDRVTALLAGTSSIREVIAFPKSGGG
VDPLTDAPAPITAAQRKESGIDAKPEKAEKAGKPADA

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=616, Percent_Identity=40.2597402597403, Blast_Score=421, Evalue=1e-118,
Organism=Homo sapiens, GI45439306, Length=293, Percent_Identity=26.6211604095563, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI226491502, Length=271, Percent_Identity=24.3542435424354, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI4758762, Length=286, Percent_Identity=25.8741258741259, Blast_Score=73, Evalue=8e-13,
Organism=Homo sapiens, GI194272210, Length=247, Percent_Identity=27.1255060728745, Blast_Score=73, Evalue=9e-13,
Organism=Homo sapiens, GI5031815, Length=247, Percent_Identity=27.1255060728745, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788173, Length=587, Percent_Identity=47.7001703577513, Blast_Score=533, Evalue=1e-152,
Organism=Escherichia coli, GI1789256, Length=284, Percent_Identity=29.2253521126761, Blast_Score=100, Evalue=5e-22,
Organism=Escherichia coli, GI1790571, Length=302, Percent_Identity=27.8145695364238, Blast_Score=99, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI32566633, Length=607, Percent_Identity=34.7611202635914, Blast_Score=317, Evalue=8e-87,
Organism=Caenorhabditis elegans, GI71984122, Length=279, Percent_Identity=27.5985663082437, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17551876, Length=271, Percent_Identity=27.3062730627306, Blast_Score=77, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71994340, Length=140, Percent_Identity=31.4285714285714, Blast_Score=73, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17535925, Length=140, Percent_Identity=31.4285714285714, Blast_Score=72, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17535927, Length=140, Percent_Identity=31.4285714285714, Blast_Score=72, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=621, Percent_Identity=32.3671497584541, Blast_Score=285, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6321807, Length=265, Percent_Identity=28.3018867924528, Blast_Score=85, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323011, Length=283, Percent_Identity=26.1484098939929, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24584738, Length=598, Percent_Identity=39.2976588628763, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI17136276, Length=239, Percent_Identity=25.5230125523013, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24640851, Length=266, Percent_Identity=26.3157894736842, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24640849, Length=272, Percent_Identity=26.8382352941176, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19921528, Length=287, Percent_Identity=26.8292682926829, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_MYCA9 (B1MCH3)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001703598.1
- ProteinModelPortal:   B1MCH3
- SMR:   B1MCH3
- EnsemblBacteria:   EBMYCT00000007938
- GeneID:   5965373
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_2865c
- GeneTree:   EBGT00050000017004
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- ProtClustDB:   PRK00476
- BioCyc:   MABS561007:MAB_2865C-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 64820; Mature: 64820

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRTHDAGSLRESNAGQRVTLAGWVARRRDHGGVIFIDLRDASGVAQVVFREDAVLEQAH
CCCCCCCCCCCCCCCCCEEEEEHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
RLRAEFCVEVSGVVEVRPEGNANDEIATGQIEVNAAELKVLNESAPLPFQLDETAGEEAR
HHHHHHHEEECCEEEECCCCCCCCCEEECEEEEEHHHEEEECCCCCCCEEECCCCCCHHH
LRYRYLDLRREGPGNAIRLRSKANAAARSVLSGHEFVEVETPTLTRSTPEGARDFLVPAR
EEEEEEEEECCCCCCEEEEECHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHCEECCE
LQPGSFYALPQSPQLFKQLLMVAGMERYYQIARCYRDEDFRADRQPEFTQLDIEMSFVDQ
ECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCC
DDVIALAEEILVALWDLVGYRVPTPIDRITYAEAMRRYGSDKPDLRFGLELVECTDYFSE
CHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
TSFRVFQAPYVGAVVMPGGADQPRRTLDGWQEFAKQRGHKGLAYVLVGEDGTLGGPVAKN
CCEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHCC
LSDAERDGLAAHVGANPGDCIFFSAGAAKSSRALLGSVRGEVAQRLGLIDPDAWAFTWVV
CCHHHHCCCEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEE
DAPLFEPADDATAAGDVAVGSGAWTAVHHAFTSPKPESENTFDTDPGSALAYAYDIVCNG
ECCCCCCCCCCCCCCCEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEEECC
HEIGGGSIRIHRRDIQERVFQVMGISNEDAQEKFGFLLDAFAFGAPPHGGIAFGWDRVTA
EEECCCEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHH
LLAGTSSIREVIAFPKSGGGVDPLTDAPAPITAAQRKESGIDAKPEKAEKAGKPADA
HHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHCCCCCCC
>Mature Secondary Structure
MLRTHDAGSLRESNAGQRVTLAGWVARRRDHGGVIFIDLRDASGVAQVVFREDAVLEQAH
CCCCCCCCCCCCCCCCCEEEEEHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
RLRAEFCVEVSGVVEVRPEGNANDEIATGQIEVNAAELKVLNESAPLPFQLDETAGEEAR
HHHHHHHEEECCEEEECCCCCCCCCEEECEEEEEHHHEEEECCCCCCCEEECCCCCCHHH
LRYRYLDLRREGPGNAIRLRSKANAAARSVLSGHEFVEVETPTLTRSTPEGARDFLVPAR
EEEEEEEEECCCCCCEEEEECHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHCEECCE
LQPGSFYALPQSPQLFKQLLMVAGMERYYQIARCYRDEDFRADRQPEFTQLDIEMSFVDQ
ECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCC
DDVIALAEEILVALWDLVGYRVPTPIDRITYAEAMRRYGSDKPDLRFGLELVECTDYFSE
CHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
TSFRVFQAPYVGAVVMPGGADQPRRTLDGWQEFAKQRGHKGLAYVLVGEDGTLGGPVAKN
CCEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHCC
LSDAERDGLAAHVGANPGDCIFFSAGAAKSSRALLGSVRGEVAQRLGLIDPDAWAFTWVV
CCHHHHCCCEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEE
DAPLFEPADDATAAGDVAVGSGAWTAVHHAFTSPKPESENTFDTDPGSALAYAYDIVCNG
ECCCCCCCCCCCCCCCEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEEECC
HEIGGGSIRIHRRDIQERVFQVMGISNEDAQEKFGFLLDAFAFGAPPHGGIAFGWDRVTA
EEECCCEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHH
LLAGTSSIREVIAFPKSGGGVDPLTDAPAPITAAQRKESGIDAKPEKAEKAGKPADA
HHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA