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The map label for this gene is carA [H]

Identifier: 169629912

GI number: 169629912

Start: 2880032

End: 2881207

Strand: Reverse

Name: carA [H]

Synonym: MAB_2828c

Alternate gene names: 169629912

Gene position: 2881207-2880032 (Counterclockwise)

Preceding gene: 169629913

Following gene: 169629911

Centisome position: 56.86

GC content: 65.05

Gene sequence:

>1176_bases
ATGACCGATGAGCCGCGTGCGCGAAGAGGATTCGGTCGTGACAAGGCGGTGCTGGTTCTCGAAGACGGGCGGGTTTTCAC
CGGTAAGCCGTTCGGCGCGGTGGGGGAAACCCTTGGTGAGGCGGTGTTCTGCACGGCCATGTCGGGGTACCAAGAGACCC
TGACCGACCCGAGCTACCACCGGCAGATCGTGGTGGCGACAGCTCCCCAGATCGGCAACACCGGGTGGAACGACGAGGAC
GACGAGAGCCGCGGCGGCAAGATCTGGGTCGCCGGATATGCGGTGCGCGATCCCTCGAACCGGGTATCGAACTGGCGCGC
CAGCGGTACCTTGGACGATGCCCTCAAGGCGCAGCATGTCGTCGGCATAGCGGGCATTGACACCCGCGCCGTGGTCCGGC
ACCTGCGTACCCGCGGATCGATGAAGGCGGGCATCTTCAGTGGCACCGTTGCCGACGCGCCCACAGATTTCCTGCTCTCC
CGCGTCAACGGGCAGCCCGCCATGCTGGGCGCCGATCTAGCCGGGGAGGTGAGCACGGCCAGCACATACACCGTGGAACC
CGATGGTGCGCACCGGTTCACGGTGGCCGCCCTGGACCTGGGCATCAAGACCAACACACCGCGTAATTTCGCGCGACGTG
GCATCCGCGTGCAGGTGGTGCCCGCCTCGATCAGCCCCGATGAGCTACTCGCGCTCGCGCCCGACGGTGTCTTTCTGTCC
AACGGTCCGGGAGATCCGGCCACCGCGGACCATGTCGTCTCGGTGACCCGGGCGGTATTGGACCGCAAGATCCCGCTGTT
CGGCATCTGCTTCGGTAACCAGTTGCTGGGCCGGGCACTTGGGCGGTCGACGTACAAGATGACGTTCGGGCATCGCGGCA
TCAACATTCCGGTCATCGACCACTCGACCGGGCGGGTTGCCATCACCGCGCAGAACCACGGTTTTGCCCTCGAAGGTGAG
GCGGGCGAGCAGTTCGACACACCGTTCGGGCGCGCCGAGGTAAGCCACACCTGCGCCAACGATGGCACGGTGGAAGGGAT
CCGGCTGTTGGACGGATCTGCTTTCTCGGTGCAGTACCACCCGGAGGCGGCGGCCGGACCTCATGACGCCGAATACCTGT
TTGATCAGTTCGCATCCGCATTGGAAACAAACTCGGAGAATCGGGGTAACCGCTGA

Upstream 100 bases:

>100_bases
GAATTCTCGCGATTCGGTGGCGGCTGCCCTCTGGCACCGAGATCGACACAGGATTTTTGGCCCGACCGCGAAGTGAATAC
GAGGAATGGAAGGTAGCGTC

Downstream 100 bases:

>100_bases
TGCCACGTCGCACAGACCTCAACCACATCCTGGTCATCGGTTCCGGACCCATCGTGATCGGCCAGGCGTGCGAGTTCGAT
TATTCGGGCACCCAGGCCTG

Product: carbamoyl-phosphate synthase, small chain (CarA)

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MTDEPRARRGFGRDKAVLVLEDGRVFTGKPFGAVGETLGEAVFCTAMSGYQETLTDPSYHRQIVVATAPQIGNTGWNDED
DESRGGKIWVAGYAVRDPSNRVSNWRASGTLDDALKAQHVVGIAGIDTRAVVRHLRTRGSMKAGIFSGTVADAPTDFLLS
RVNGQPAMLGADLAGEVSTASTYTVEPDGAHRFTVAALDLGIKTNTPRNFARRGIRVQVVPASISPDELLALAPDGVFLS
NGPGDPATADHVVSVTRAVLDRKIPLFGICFGNQLLGRALGRSTYKMTFGHRGINIPVIDHSTGRVAITAQNHGFALEGE
AGEQFDTPFGRAEVSHTCANDGTVEGIRLLDGSAFSVQYHPEAAAGPHDAEYLFDQFASALETNSENRGNR

Sequences:

>Translated_391_residues
MTDEPRARRGFGRDKAVLVLEDGRVFTGKPFGAVGETLGEAVFCTAMSGYQETLTDPSYHRQIVVATAPQIGNTGWNDED
DESRGGKIWVAGYAVRDPSNRVSNWRASGTLDDALKAQHVVGIAGIDTRAVVRHLRTRGSMKAGIFSGTVADAPTDFLLS
RVNGQPAMLGADLAGEVSTASTYTVEPDGAHRFTVAALDLGIKTNTPRNFARRGIRVQVVPASISPDELLALAPDGVFLS
NGPGDPATADHVVSVTRAVLDRKIPLFGICFGNQLLGRALGRSTYKMTFGHRGINIPVIDHSTGRVAITAQNHGFALEGE
AGEQFDTPFGRAEVSHTCANDGTVEGIRLLDGSAFSVQYHPEAAAGPHDAEYLFDQFASALETNSENRGNR
>Mature_390_residues
TDEPRARRGFGRDKAVLVLEDGRVFTGKPFGAVGETLGEAVFCTAMSGYQETLTDPSYHRQIVVATAPQIGNTGWNDEDD
ESRGGKIWVAGYAVRDPSNRVSNWRASGTLDDALKAQHVVGIAGIDTRAVVRHLRTRGSMKAGIFSGTVADAPTDFLLSR
VNGQPAMLGADLAGEVSTASTYTVEPDGAHRFTVAALDLGIKTNTPRNFARRGIRVQVVPASISPDELLALAPDGVFLSN
GPGDPATADHVVSVTRAVLDRKIPLFGICFGNQLLGRALGRSTYKMTFGHRGINIPVIDHSTGRVAITAQNHGFALEGEA
GEQFDTPFGRAEVSHTCANDGTVEGIRLLDGSAFSVQYHPEAAAGPHDAEYLFDQFASALETNSENRGNR

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=380, Percent_Identity=41.5789473684211, Blast_Score=238, Evalue=8e-63,
Organism=Homo sapiens, GI169790915, Length=380, Percent_Identity=37.3684210526316, Blast_Score=206, Evalue=3e-53,
Organism=Homo sapiens, GI21361331, Length=380, Percent_Identity=37.3684210526316, Blast_Score=206, Evalue=4e-53,
Organism=Escherichia coli, GI1786215, Length=381, Percent_Identity=44.8818897637795, Blast_Score=298, Evalue=5e-82,
Organism=Caenorhabditis elegans, GI193204318, Length=378, Percent_Identity=38.3597883597884, Blast_Score=225, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6322331, Length=397, Percent_Identity=35.5163727959698, Blast_Score=217, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6324878, Length=382, Percent_Identity=37.17277486911, Blast_Score=215, Evalue=9e-57,
Organism=Drosophila melanogaster, GI45555749, Length=389, Percent_Identity=40.3598971722365, Blast_Score=231, Evalue=7e-61,
Organism=Drosophila melanogaster, GI24642586, Length=388, Percent_Identity=39.9484536082474, Blast_Score=230, Evalue=1e-60,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 41675; Mature: 41544

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDEPRARRGFGRDKAVLVLEDGRVFTGKPFGAVGETLGEAVFCTAMSGYQETLTDPSYH
CCCCCCHHHCCCCCCEEEEEECCEEEECCCCHHHHHHHHHEEEEEECCCHHHHCCCCCCC
RQIVVATAPQIGNTGWNDEDDESRGGKIWVAGYAVRDPSNRVSNWRASGTLDDALKAQHV
CEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEEEECCHHHHCCCCCCCCHHHHHHHHHE
VGIAGIDTRAVVRHLRTRGSMKAGIFSGTVADAPTDFLLSRVNGQPAMLGADLAGEVSTA
EEEECCCHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHCCCCCEEEECCCCCCCCCC
STYTVEPDGAHRFTVAALDLGIKTNTPRNFARRGIRVQVVPASISPDELLALAPDGVFLS
EEEEECCCCCCEEEEEEEEECCCCCCCHHHHHCCCEEEEEECCCCCCCEEEECCCCEEEE
NGPGDPATADHVVSVTRAVLDRKIPLFGICFGNQLLGRALGRSTYKMTFGHRGINIPVID
CCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHCCCEEEEEECCCCCEEEEEE
HSTGRVAITAQNHGFALEGEAGEQFDTPFGRAEVSHTCANDGTVEGIRLLDGSAFSVQYH
CCCCEEEEEECCCCEEEECCCCCCCCCCCCCCHHHHCCCCCCCCCEEEEECCCEEEEEEC
PEAAAGPHDAEYLFDQFASALETNSENRGNR
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TDEPRARRGFGRDKAVLVLEDGRVFTGKPFGAVGETLGEAVFCTAMSGYQETLTDPSYH
CCCCCHHHCCCCCCEEEEEECCEEEECCCCHHHHHHHHHEEEEEECCCHHHHCCCCCCC
RQIVVATAPQIGNTGWNDEDDESRGGKIWVAGYAVRDPSNRVSNWRASGTLDDALKAQHV
CEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEEEECCHHHHCCCCCCCCHHHHHHHHHE
VGIAGIDTRAVVRHLRTRGSMKAGIFSGTVADAPTDFLLSRVNGQPAMLGADLAGEVSTA
EEEECCCHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHCCCCCEEEECCCCCCCCCC
STYTVEPDGAHRFTVAALDLGIKTNTPRNFARRGIRVQVVPASISPDELLALAPDGVFLS
EEEEECCCCCCEEEEEEEEECCCCCCCHHHHHCCCEEEEEECCCCCCCEEEECCCCEEEE
NGPGDPATADHVVSVTRAVLDRKIPLFGICFGNQLLGRALGRSTYKMTFGHRGINIPVID
CCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHCCCEEEEEECCCCCEEEEEE
HSTGRVAITAQNHGFALEGEAGEQFDTPFGRAEVSHTCANDGTVEGIRLLDGSAFSVQYH
CCCCEEEEEECCCCEEEECCCCCCCCCCCCCCHHHHCCCCCCCCCEEEEECCCEEEEEEC
PEAAAGPHDAEYLFDQFASALETNSENRGNR
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA