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The map label for this gene is glgX [H]

Identifier: 169629775

GI number: 169629775

Start: 2733409

End: 2735550

Strand: Direct

Name: glgX [H]

Synonym: MAB_2690

Alternate gene names: 169629775

Gene position: 2733409-2735550 (Clockwise)

Preceding gene: 169629774

Following gene: 169629778

Centisome position: 53.94

GC content: 62.23

Gene sequence:

>2142_bases
ATGACCCTGCCACCCCTTCAGGTATGGCCGGGTAACCCATATCCGCTGGGCGCCACCTATGACGGCGCGGGCACCAATTT
CTCGTTGTTCTCCGAGGTGGCCACGTCCGTCGAACTGTGCCTGATCGCCAAGGACGGGACCGAAACCCGGATCCCCCTGG
ATGAGGTCGATGGATATGTGTGGCACTGCTATCTGCCGACAATCTCCCCGGGCCAGCGTTACGGCTTCCGTGTGCACGGA
CCGTGGGATCCGGAAAGCGGGCATCGGTGCGACCCCAGCAAGCTGCTGCTCGACCCGTATGGAAAAGCCTTTCACGGCGA
GTTCGGTTACGTCCCCGATACCGCGCCGCCGTTGCTCTCCTATCAGATAGATCCCGTGGACACCGAGACGCTCGTGCCAC
GGGACTCGCTGGGGCGCACCATGACCACTGTCGTCATCAACCCCTACTTCGACTGGGGTTCGGACCGCCGACCACGCACC
CCGTACCACGAGACAGTCATCTACGAGGCACACGTCAAGGGCATGACGCAGACTCACCCCGGGATACCCGAGGAACTTCG
GGGCACCTATGCGGGGCTGGCGCATCCCGCGGTGATAGACCACCTGCATTCCCTCGGTGTCACCGCCATCGAACTCATGC
CGGTACACCAGTTCTTCCACGACAGCCGGCTGATCGCGCTGGGGCTGCGAAATTACTGGGGATACAACACCTTCGGGTAC
CTCGCACCGCATGCCGGATACGCGTCTTCACCACATGCCGGCGGAGCAGTGGCCGAGTTCAAGGCAATGGTGCGCGCACT
CCACGAGGCAGGCATCGAGGTGATTCTCGACGTCGTCTACAACCACACCGCCGAGGGCGACCATATCGGACCGACACTGA
GCTTCCGAGGTATCGATAACCGCGCCTACTACAAACTCAACGACGACAACCTCGCCCGGTATACCGACTACACCGGAACC
GGGAACAGCCTCAACGCCCGTAACCCGCACACGTTGCAGCTCATTATGGACTCGCTGCGCTACTGGGTCACCGAGATGCA
CGTCGACGGTTTCCGTTTCGATTTGGCCTCCACCCTGGCGCGGGAACTGCACGATGTCGACAGGCTCTCGGCCTTCTTCG
ATCTGGTGCAGCAGGATCCGATCGTCAGCCAGGTCAAGCTGATCGCCGAGCCGTGGGATATCGGCGAAGGCGGATATCAG
GTGGGCAACTTCCCCGGGCTGTGGACCGAATGGAACGGAAAGTTCCGCGACACCGTCCGCGATTATTGGCGTGGACAGCC
AGCCACCCTCGGAGAATTCGCGTCGCGCCTCACCGGTTCATCTGACCTGTACGAAGCCACCGGGCGCCGCCCCAGCGCGA
GTATCAACTTCGTCACCGCTCACGACGGATTCACCCTGCGAGACCTGGTCTCCTACAACGAGAAACACAACGAGGCCAAC
GGCGAGAACAATCAAGACGGCGAAACCTACAACCGGTCCTGGAACTGCGGTGTGGAAGGGCCCACCGATGATCCGCAGAT
TCTCGCGCTGCGCGCCCGGCAGATGCGCAACATCTTCGCAACCCTGGTACTGAGCCAGGGCACGCCGATGCTGTCACACG
GAGACGAGATCGGCCGTACGCAGCAAGGCAACAACAATGTCTACTGCCAGGATTCGGCGCTGTCCTGGATGGATTGGGAA
CTCGCCACCGCGAACGCCGACCTGCTGCAGTTCGCACGTAGCGTGATCGCGCTACGTAAACAACACCCGGTGTTTCGGCG
CCGGCGGTTCTTCGCCGGGCGTCCGATCCGGGAAGGCGAGGAAGTGCGCGATATCGCGTGGCTCACCCCCGCGGGCGAGG
AAATGACCACTGCCGACTGGGACAGCGGATTCGGCAAGAGCCTGGCCGTATTCCTCAACGGTGACGCAATCCCCGAGCCG
AACGCGCGCGGCGAGCGAGTATCCGGCGACTCGTTCCTGTTGTGCTTCAACGCATACGACGAACCACTGGATTTCGTGAC
GCCCGACGGCGATTACGCGACCCAATGGACCGCCGTACTGGACACCGCCACACCCAATGGCCAATGCTCGACCGTGGTCA
AGGCTGGCGAAGCAGTGCGCGTACAAGACCGCGCCCTAGTGGTGCTGCGTAAAAGCGGGTAG

Upstream 100 bases:

>100_bases
CTGGGCCGCCAGATCGCGCTCGCCACCGGATGGTGGCGCCCGGCCCAACCGGGCCAGTGACGCGCGCGATCCGGGCCCTC
GACCGATAGGGTCTATCAGC

Downstream 100 bases:

>100_bases
CCGCCCTATGTCAGATAGCGGAAGGCCGGCGAGTCCGGCTCCAGCCGTTCGATATCCAGCCCGGAAAACCGCATCCGATC
CAGCAGACCCGACAGGTCAT

Product: glycogen operon protein GlgX-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 713; Mature: 712

Protein sequence:

>713_residues
MTLPPLQVWPGNPYPLGATYDGAGTNFSLFSEVATSVELCLIAKDGTETRIPLDEVDGYVWHCYLPTISPGQRYGFRVHG
PWDPESGHRCDPSKLLLDPYGKAFHGEFGYVPDTAPPLLSYQIDPVDTETLVPRDSLGRTMTTVVINPYFDWGSDRRPRT
PYHETVIYEAHVKGMTQTHPGIPEELRGTYAGLAHPAVIDHLHSLGVTAIELMPVHQFFHDSRLIALGLRNYWGYNTFGY
LAPHAGYASSPHAGGAVAEFKAMVRALHEAGIEVILDVVYNHTAEGDHIGPTLSFRGIDNRAYYKLNDDNLARYTDYTGT
GNSLNARNPHTLQLIMDSLRYWVTEMHVDGFRFDLASTLARELHDVDRLSAFFDLVQQDPIVSQVKLIAEPWDIGEGGYQ
VGNFPGLWTEWNGKFRDTVRDYWRGQPATLGEFASRLTGSSDLYEATGRRPSASINFVTAHDGFTLRDLVSYNEKHNEAN
GENNQDGETYNRSWNCGVEGPTDDPQILALRARQMRNIFATLVLSQGTPMLSHGDEIGRTQQGNNNVYCQDSALSWMDWE
LATANADLLQFARSVIALRKQHPVFRRRRFFAGRPIREGEEVRDIAWLTPAGEEMTTADWDSGFGKSLAVFLNGDAIPEP
NARGERVSGDSFLLCFNAYDEPLDFVTPDGDYATQWTAVLDTATPNGQCSTVVKAGEAVRVQDRALVVLRKSG

Sequences:

>Translated_713_residues
MTLPPLQVWPGNPYPLGATYDGAGTNFSLFSEVATSVELCLIAKDGTETRIPLDEVDGYVWHCYLPTISPGQRYGFRVHG
PWDPESGHRCDPSKLLLDPYGKAFHGEFGYVPDTAPPLLSYQIDPVDTETLVPRDSLGRTMTTVVINPYFDWGSDRRPRT
PYHETVIYEAHVKGMTQTHPGIPEELRGTYAGLAHPAVIDHLHSLGVTAIELMPVHQFFHDSRLIALGLRNYWGYNTFGY
LAPHAGYASSPHAGGAVAEFKAMVRALHEAGIEVILDVVYNHTAEGDHIGPTLSFRGIDNRAYYKLNDDNLARYTDYTGT
GNSLNARNPHTLQLIMDSLRYWVTEMHVDGFRFDLASTLARELHDVDRLSAFFDLVQQDPIVSQVKLIAEPWDIGEGGYQ
VGNFPGLWTEWNGKFRDTVRDYWRGQPATLGEFASRLTGSSDLYEATGRRPSASINFVTAHDGFTLRDLVSYNEKHNEAN
GENNQDGETYNRSWNCGVEGPTDDPQILALRARQMRNIFATLVLSQGTPMLSHGDEIGRTQQGNNNVYCQDSALSWMDWE
LATANADLLQFARSVIALRKQHPVFRRRRFFAGRPIREGEEVRDIAWLTPAGEEMTTADWDSGFGKSLAVFLNGDAIPEP
NARGERVSGDSFLLCFNAYDEPLDFVTPDGDYATQWTAVLDTATPNGQCSTVVKAGEAVRVQDRALVVLRKSG
>Mature_712_residues
TLPPLQVWPGNPYPLGATYDGAGTNFSLFSEVATSVELCLIAKDGTETRIPLDEVDGYVWHCYLPTISPGQRYGFRVHGP
WDPESGHRCDPSKLLLDPYGKAFHGEFGYVPDTAPPLLSYQIDPVDTETLVPRDSLGRTMTTVVINPYFDWGSDRRPRTP
YHETVIYEAHVKGMTQTHPGIPEELRGTYAGLAHPAVIDHLHSLGVTAIELMPVHQFFHDSRLIALGLRNYWGYNTFGYL
APHAGYASSPHAGGAVAEFKAMVRALHEAGIEVILDVVYNHTAEGDHIGPTLSFRGIDNRAYYKLNDDNLARYTDYTGTG
NSLNARNPHTLQLIMDSLRYWVTEMHVDGFRFDLASTLARELHDVDRLSAFFDLVQQDPIVSQVKLIAEPWDIGEGGYQV
GNFPGLWTEWNGKFRDTVRDYWRGQPATLGEFASRLTGSSDLYEATGRRPSASINFVTAHDGFTLRDLVSYNEKHNEANG
ENNQDGETYNRSWNCGVEGPTDDPQILALRARQMRNIFATLVLSQGTPMLSHGDEIGRTQQGNNNVYCQDSALSWMDWEL
ATANADLLQFARSVIALRKQHPVFRRRRFFAGRPIREGEEVRDIAWLTPAGEEMTTADWDSGFGKSLAVFLNGDAIPEPN
ARGERVSGDSFLLCFNAYDEPLDFVTPDGDYATQWTAVLDTATPNGQCSTVVKAGEAVRVQDRALVVLRKSG

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=692, Percent_Identity=45.8092485549133, Blast_Score=572, Evalue=1e-164,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 79444; Mature: 79313

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPPLQVWPGNPYPLGATYDGAGTNFSLFSEVATSVELCLIAKDGTETRIPLDEVDGYV
CCCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHCCEEEEEEECCCCCCCCCHHHCCCEE
WHCYLPTISPGQRYGFRVHGPWDPESGHRCDPSKLLLDPYGKAFHGEFGYVPDTAPPLLS
EEEECCCCCCCCCCCEEEECCCCCCCCCCCCCHHEEECCCCCCCCCCCCCCCCCCCCCEE
YQIDPVDTETLVPRDSLGRTMTTVVINPYFDWGSDRRPRTPYHETVIYEAHVKGMTQTHP
EEECCCCCCCCCCHHHCCCEEEEEEEECCCCCCCCCCCCCCCHHEEEEEEHHCCCCCCCC
GIPEELRGTYAGLAHPAVIDHLHSLGVTAIELMPVHQFFHDSRLIALGLRNYWGYNTFGY
CCCHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEHHHHHHCCCCEEEEEHHHHCCCCCCCC
LAPHAGYASSPHAGGAVAEFKAMVRALHEAGIEVILDVVYNHTAEGDHIGPTLSFRGIDN
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEECCCC
RAYYKLNDDNLARYTDYTGTGNSLNARNPHTLQLIMDSLRYWVTEMHVDGFRFDLASTLA
CEEEEECCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
RELHDVDRLSAFFDLVQQDPIVSQVKLIAEPWDIGEGGYQVGNFPGLWTEWNGKFRDTVR
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHH
DYWRGQPATLGEFASRLTGSSDLYEATGRRPSASINFVTAHDGFTLRDLVSYNEKHNEAN
HHHCCCCCCHHHHHHHHCCCCHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCC
GENNQDGETYNRSWNCGVEGPTDDPQILALRARQMRNIFATLVLSQGTPMLSHGDEIGRT
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCC
QQGNNNVYCQDSALSWMDWELATANADLLQFARSVIALRKQHPVFRRRRFFAGRPIREGE
CCCCCCEEECCCCCCEECEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCC
EVRDIAWLTPAGEEMTTADWDSGFGKSLAVFLNGDAIPEPNARGERVSGDSFLLCFNAYD
HHHHEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEECCCCEEEEEECCC
EPLDFVTPDGDYATQWTAVLDTATPNGQCSTVVKAGEAVRVQDRALVVLRKSG
CCCCCCCCCCCCCEEEEEEEECCCCCCCCHHEEECCCEEEEECCEEEEEEECC
>Mature Secondary Structure 
TLPPLQVWPGNPYPLGATYDGAGTNFSLFSEVATSVELCLIAKDGTETRIPLDEVDGYV
CCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHCCEEEEEEECCCCCCCCCHHHCCCEE
WHCYLPTISPGQRYGFRVHGPWDPESGHRCDPSKLLLDPYGKAFHGEFGYVPDTAPPLLS
EEEECCCCCCCCCCCEEEECCCCCCCCCCCCCHHEEECCCCCCCCCCCCCCCCCCCCCEE
YQIDPVDTETLVPRDSLGRTMTTVVINPYFDWGSDRRPRTPYHETVIYEAHVKGMTQTHP
EEECCCCCCCCCCHHHCCCEEEEEEEECCCCCCCCCCCCCCCHHEEEEEEHHCCCCCCCC
GIPEELRGTYAGLAHPAVIDHLHSLGVTAIELMPVHQFFHDSRLIALGLRNYWGYNTFGY
CCCHHHHHHHHCCCCHHHHHHHHHCCCEEEEEEHHHHHHCCCCEEEEEHHHHCCCCCCCC
LAPHAGYASSPHAGGAVAEFKAMVRALHEAGIEVILDVVYNHTAEGDHIGPTLSFRGIDN
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEECCCC
RAYYKLNDDNLARYTDYTGTGNSLNARNPHTLQLIMDSLRYWVTEMHVDGFRFDLASTLA
CEEEEECCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
RELHDVDRLSAFFDLVQQDPIVSQVKLIAEPWDIGEGGYQVGNFPGLWTEWNGKFRDTVR
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHH
DYWRGQPATLGEFASRLTGSSDLYEATGRRPSASINFVTAHDGFTLRDLVSYNEKHNEAN
HHHCCCCCCHHHHHHHHCCCCHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCC
GENNQDGETYNRSWNCGVEGPTDDPQILALRARQMRNIFATLVLSQGTPMLSHGDEIGRT
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCC
QQGNNNVYCQDSALSWMDWELATANADLLQFARSVIALRKQHPVFRRRRFFAGRPIREGE
CCCCCCEEECCCCCCEECEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCC
EVRDIAWLTPAGEEMTTADWDSGFGKSLAVFLNGDAIPEPNARGERVSGDSFLLCFNAYD
HHHHEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEECCCCEEEEEECCC
EPLDFVTPDGDYATQWTAVLDTATPNGQCSTVVKAGEAVRVQDRALVVLRKSG
CCCCCCCCCCCCCEEEEEEEECCCCCCCCHHEEECCCEEEEECCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]