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The map label for this gene is nadB [H]

Identifier: 169629758

GI number: 169629758

Start: 2714146

End: 2715717

Strand: Reverse

Name: nadB [H]

Synonym: MAB_2673c

Alternate gene names: 169629758

Gene position: 2715717-2714146 (Counterclockwise)

Preceding gene: 169629759

Following gene: 169629757

Centisome position: 53.59

GC content: 68.19

Gene sequence:

>1572_bases
ATGACCATGCCGGCGGGGACCACCTGGCGCACTGACGCAGATGTGGTCGTCGTCGGTGCGGGTGTCGCCGGGCTTGCGGC
CGCCTTGGCGGTGCACCGGCGTGGCCGCAAGGTCGTGGTACTGAGCAAGGTCGGTGCGACCGCCACGCATTTCGCGCAGG
GCGGGATCGCCGTGGTGCTCCCGGATTCGCAGGATTCCATCGAGGCGCATGTACTGGACACGCTGAGCGCCGGAGCTGGG
CTATGTGATGCCGAGGCGGTCCGGTCCATCGTGTCCGACGGATACCGGGCGGTACGCGATCTGGTCGCCGACGGCGCGCG
ATTCGATGAATCGCGTTCGGGTCAGTGGGCGTTGACCCGCGAGGGCGGGCATAGCGCGCGGCGCATCATCCACGCCGGTG
GCGATGCCACGGGCGCGGAGGTGCAGCGCGCGCTTGATCACGCGGCAGCGATGTTGGACGTCCGCCGCAGCCACGTCGTG
CACGACATCCTCGTCGGGCCCGAGGGCGTGGGCGGCGTGCTCGTGGGAAACAGCGACGGGATTGGGGTGATCCACGCGGG
AGCCGTGGTGCTCGCCACCGGCGGACTGGGACATCTATATTCGGCCACCACCAATCCGGAGGGATCGACCGGCGATGGCA
TCGCATTGGCGCTGCGCGCGGGCGTATCGGTGGCCGACATCGAATTCATCCAGTTCCACCCGACGATGCTGCACACCCCG
GATGCGGCCGGATGCCGGCCCCTGATCAGCGAAGCGGTCCGTGGCGAAGGTGCCGTCTTGGTTGACGCACGCGGGGATTC
GGTGACCGCGGGTGTGCATCCGCAGGGGGATCTGGCCCCGCGCGACGTGGTGGCCGGGGCCATCGCGCGGCGACTACGTG
AGAGCGGAGACCAATGTGTTTACCTCGATGCCCGCGCCATCGGTAACTTTGCGATCCGATTTCCCACCATCACCGCCGCG
TGCGCGGCGGCCGGGATAGATCCGTCGGTCCAGCTGATCCCGGTAGTGCCGGGGGCGCATTACAGCTGCGGCGGTGTCGT
CACCGATCCGTACGGTCACACCGAGGTGGCCGGGTTGTTCGCCGCGGGCGAGGTCGCGCGCACCGGTATGCATGGCGCAA
ACCGGTTGGCTTCCAACAGTTTGTTGGAAGGTTTGGTGGTGGGTAGGCGTGCCGGGGAAGCTGCTGCGCGGATCGCTGAC
CGCGCCGGTGTGGTGGATGAACGCGACCCCGCACCACCGCGTCTGGATCGCCGAAGGTTGCAGTCGGCGATGACTCGGTG
GGCGTCGGTGCTGAGGGATGCCGACGGGCTCGGCCTGTTGGAGGCCGAACTACGTTGCGGCACTGTGTCACCGGTTGACC
CCGCTCGCATGACACCGCGCGACGTCGAGGATGCGGCGCTTACCCTGACAGCGCAGGCTGTGGCGACGGCTGCCGCGGCA
CGCACAGAATCGCGCGGCTGCCATGCCCGTTCGGACTGGCCCCGAACCGATTCGCATTTTGCCGTCAGCCTTCCGGTGCG
CCGCGACGCGGATGGATGCGAAGTTGCGCTACCGTCTGGAGTGCCATGTTGA

Upstream 100 bases:

>100_bases
TGGAGGGGCGCGATGAGGTGGATGTCGATCCGGACACCGCGCGCCTGGCCCGCCGCAGCGTCCAGCGGATGATCGAGATC
GGACAACCCGGCGGCGGAGA

Downstream 100 bases:

>100_bases
CCGGAACACTGACGGAAACCGAACTCGCGGAGGCGCGTTCGGTGGTGGAGCGCGCTCTTGCCGAGGACCTGCATTACGGC
CCGGACATCACCAGTCGGGC

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MTMPAGTTWRTDADVVVVGAGVAGLAAALAVHRRGRKVVVLSKVGATATHFAQGGIAVVLPDSQDSIEAHVLDTLSAGAG
LCDAEAVRSIVSDGYRAVRDLVADGARFDESRSGQWALTREGGHSARRIIHAGGDATGAEVQRALDHAAAMLDVRRSHVV
HDILVGPEGVGGVLVGNSDGIGVIHAGAVVLATGGLGHLYSATTNPEGSTGDGIALALRAGVSVADIEFIQFHPTMLHTP
DAAGCRPLISEAVRGEGAVLVDARGDSVTAGVHPQGDLAPRDVVAGAIARRLRESGDQCVYLDARAIGNFAIRFPTITAA
CAAAGIDPSVQLIPVVPGAHYSCGGVVTDPYGHTEVAGLFAAGEVARTGMHGANRLASNSLLEGLVVGRRAGEAAARIAD
RAGVVDERDPAPPRLDRRRLQSAMTRWASVLRDADGLGLLEAELRCGTVSPVDPARMTPRDVEDAALTLTAQAVATAAAA
RTESRGCHARSDWPRTDSHFAVSLPVRRDADGCEVALPSGVPC

Sequences:

>Translated_523_residues
MTMPAGTTWRTDADVVVVGAGVAGLAAALAVHRRGRKVVVLSKVGATATHFAQGGIAVVLPDSQDSIEAHVLDTLSAGAG
LCDAEAVRSIVSDGYRAVRDLVADGARFDESRSGQWALTREGGHSARRIIHAGGDATGAEVQRALDHAAAMLDVRRSHVV
HDILVGPEGVGGVLVGNSDGIGVIHAGAVVLATGGLGHLYSATTNPEGSTGDGIALALRAGVSVADIEFIQFHPTMLHTP
DAAGCRPLISEAVRGEGAVLVDARGDSVTAGVHPQGDLAPRDVVAGAIARRLRESGDQCVYLDARAIGNFAIRFPTITAA
CAAAGIDPSVQLIPVVPGAHYSCGGVVTDPYGHTEVAGLFAAGEVARTGMHGANRLASNSLLEGLVVGRRAGEAAARIAD
RAGVVDERDPAPPRLDRRRLQSAMTRWASVLRDADGLGLLEAELRCGTVSPVDPARMTPRDVEDAALTLTAQAVATAAAA
RTESRGCHARSDWPRTDSHFAVSLPVRRDADGCEVALPSGVPC
>Mature_522_residues
TMPAGTTWRTDADVVVVGAGVAGLAAALAVHRRGRKVVVLSKVGATATHFAQGGIAVVLPDSQDSIEAHVLDTLSAGAGL
CDAEAVRSIVSDGYRAVRDLVADGARFDESRSGQWALTREGGHSARRIIHAGGDATGAEVQRALDHAAAMLDVRRSHVVH
DILVGPEGVGGVLVGNSDGIGVIHAGAVVLATGGLGHLYSATTNPEGSTGDGIALALRAGVSVADIEFIQFHPTMLHTPD
AAGCRPLISEAVRGEGAVLVDARGDSVTAGVHPQGDLAPRDVVAGAIARRLRESGDQCVYLDARAIGNFAIRFPTITAAC
AAAGIDPSVQLIPVVPGAHYSCGGVVTDPYGHTEVAGLFAAGEVARTGMHGANRLASNSLLEGLVVGRRAGEAAARIADR
AGVVDERDPAPPRLDRRRLQSAMTRWASVLRDADGLGLLEAELRCGTVSPVDPARMTPRDVEDAALTLTAQAVATAAAAR
TESRGCHARSDWPRTDSHFAVSLPVRRDADGCEVALPSGVPC

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=541, Percent_Identity=30.6839186691312, Blast_Score=172, Evalue=1e-42,
Organism=Escherichia coli, GI1788928, Length=526, Percent_Identity=40.8745247148289, Blast_Score=306, Evalue=3e-84,
Organism=Escherichia coli, GI1790597, Length=538, Percent_Identity=34.7583643122677, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1786942, Length=555, Percent_Identity=32.7927927927928, Blast_Score=191, Evalue=7e-50,
Organism=Caenorhabditis elegans, GI17505833, Length=539, Percent_Identity=29.3135435992579, Blast_Score=171, Evalue=9e-43,
Organism=Caenorhabditis elegans, GI17550100, Length=543, Percent_Identity=30.3867403314917, Blast_Score=166, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6322701, Length=556, Percent_Identity=31.4748201438849, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6322416, Length=553, Percent_Identity=31.4647377938517, Blast_Score=167, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6322511, Length=477, Percent_Identity=24.5283018867925, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6320788, Length=475, Percent_Identity=26.3157894736842, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI17137288, Length=557, Percent_Identity=31.4183123877917, Blast_Score=176, Evalue=5e-44,
Organism=Drosophila melanogaster, GI24655642, Length=557, Percent_Identity=31.4183123877917, Blast_Score=176, Evalue=5e-44,
Organism=Drosophila melanogaster, GI24655647, Length=557, Percent_Identity=31.4183123877917, Blast_Score=176, Evalue=5e-44,
Organism=Drosophila melanogaster, GI24663005, Length=553, Percent_Identity=30.1989150090416, Blast_Score=164, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 53712; Mature: 53581

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMPAGTTWRTDADVVVVGAGVAGLAAALAVHRRGRKVVVLSKVGATATHFAQGGIAVVL
CCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHCCCCEEEEE
PDSQDSIEAHVLDTLSAGAGLCDAEAVRSIVSDGYRAVRDLVADGARFDESRSGQWALTR
CCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
EGGHSARRIIHAGGDATGAEVQRALDHAAAMLDVRRSHVVHDILVGPEGVGGVLVGNSDG
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEECCCC
IGVIHAGAVVLATGGLGHLYSATTNPEGSTGDGIALALRAGVSVADIEFIQFHPTMLHTP
EEEEECCEEEEEECCCCCEEECCCCCCCCCCCCEEEEEECCCCEEEEEEEEECCCEEECC
DAAGCRPLISEAVRGEGAVLVDARGDSVTAGVHPQGDLAPRDVVAGAIARRLRESGDQCV
CCCCCHHHHHHHHCCCCEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEE
YLDARAIGNFAIRFPTITAACAAAGIDPSVQLIPVVPGAHYSCGGVVTDPYGHTEVAGLF
EEEHHHHCCEEEECCHHHHHHHHCCCCCCCEEEEECCCCCCCCCCEEECCCCCHHHHHHH
AAGEVARTGMHGANRLASNSLLEGLVVGRRAGEAAARIADRAGVVDERDPAPPRLDRRRL
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHH
QSAMTRWASVLRDADGLGLLEAELRCGTVSPVDPARMTPRDVEDAALTLTAQAVATAAAA
HHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHEEEHHHHHHHHHHH
RTESRGCHARSDWPRTDSHFAVSLPVRRDADGCEVALPSGVPC
HHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEECCCCCCC
>Mature Secondary Structure 
TMPAGTTWRTDADVVVVGAGVAGLAAALAVHRRGRKVVVLSKVGATATHFAQGGIAVVL
CCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHCCCCEEEEE
PDSQDSIEAHVLDTLSAGAGLCDAEAVRSIVSDGYRAVRDLVADGARFDESRSGQWALTR
CCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
EGGHSARRIIHAGGDATGAEVQRALDHAAAMLDVRRSHVVHDILVGPEGVGGVLVGNSDG
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEECCCC
IGVIHAGAVVLATGGLGHLYSATTNPEGSTGDGIALALRAGVSVADIEFIQFHPTMLHTP
EEEEECCEEEEEECCCCCEEECCCCCCCCCCCCEEEEEECCCCEEEEEEEEECCCEEECC
DAAGCRPLISEAVRGEGAVLVDARGDSVTAGVHPQGDLAPRDVVAGAIARRLRESGDQCV
CCCCCHHHHHHHHCCCCEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEE
YLDARAIGNFAIRFPTITAACAAAGIDPSVQLIPVVPGAHYSCGGVVTDPYGHTEVAGLF
EEEHHHHCCEEEECCHHHHHHHHCCCCCCCEEEEECCCCCCCCCCEEECCCCCHHHHHHH
AAGEVARTGMHGANRLASNSLLEGLVVGRRAGEAAARIADRAGVVDERDPAPPRLDRRRL
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHH
QSAMTRWASVLRDADGLGLLEAELRCGTVSPVDPARMTPRDVEDAALTLTAQAVATAAAA
HHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHEEEHHHHHHHHHHH
RTESRGCHARSDWPRTDSHFAVSLPVRRDADGCEVALPSGVPC
HHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]