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The map label for this gene is hisF

Identifier: 169629749

GI number: 169629749

Start: 2706673

End: 2707446

Strand: Reverse

Name: hisF

Synonym: MAB_2664c

Alternate gene names: 169629749

Gene position: 2707446-2706673 (Counterclockwise)

Preceding gene: 169629750

Following gene: 169629748

Centisome position: 53.43

GC content: 66.02

Gene sequence:

>774_bases
ATGAGTGTTGCCACCCGCGTCATCGCATGCCTGGACGTCGACGACGGCCGGGTGGTCAAGGGCGTGAATTTCGAAAACCT
CCGGGATGCGGGCGATCCGGTCGAGTTGGCGGCCGCGTACGACGCCGAGGGCGTGGACGAGCTGACCTTCCTGGACGTCA
CCGCGTCGTCGTCTGGGCGCGCCACGATGTTGGACGTGGTCCGGCGTACGGCTGAGCAGGTGTTCATACCGCTGACGGTG
GGTGGCGGCGTGCGATCGGTCGATGACGTCAATGTGTTGTTGCGGGCCGGTGCGGACAAAGTAGGTGTGAACACCGCGGC
CATCGCACGCCCGGAACTGCTGGCCGAGTTGGCGCAGCGGTTCGGATCGCAGTGCATCGTGCTGAGTGTCGACGCCCGAC
GCGTCCGTGATGGCGATGTGCCCACCTCGTCCGGGTGGGAGGTGACCACTCATGGCGGACGCCGGGGCACCGGTATCGAC
GCGATCGAATGGACAAGTCGTGGAGCAGAATTAGGGGTAGGCGAGATCTTGCTCAATTCCATGGACGCCGACGGCACCAA
GGCGGGATTCGACCTGGAGATGATCGCTGCGGCGCGCGCCGCGGTCGACGTCCCGGTGATTGCCAGCGGTGGAGCGGGTG
CCATCGGCCACTTCGCGCCCGCGGTGCAGGCCGGTGCCGATGCGGTGTTGGCGGCAAGTGTCTTTCACTTCCGCGAGCTG
ACCATCGGCGAGGTGAAGGCCGCGATGGCAGCAGAAGGGATCACGGTGCGATGA

Upstream 100 bases:

>100_bases
CCTGGACCCCGGAATCGCCCTCGCTACTGGTGGCGGCACCGGGCGTGCAGGAGCAGATCTTGGAAGTACTGGCTGAGGTC
GGAGATCCGGAGGACTACCG

Downstream 100 bases:

>100_bases
GCGAACTCGCTCCAGATATCGCCGCCCGCCTGAAGCGCGATGGCGCTGGTTTGGTGACCGCGGTGGTGCAGGAACACGGC
ACGGGCACCGTCCTCATGGT

Product: imidazole glycerol phosphate synthase, cyclase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MSVATRVIACLDVDDGRVVKGVNFENLRDAGDPVELAAAYDAEGVDELTFLDVTASSSGRATMLDVVRRTAEQVFIPLTV
GGGVRSVDDVNVLLRAGADKVGVNTAAIARPELLAELAQRFGSQCIVLSVDARRVRDGDVPTSSGWEVTTHGGRRGTGID
AIEWTSRGAELGVGEILLNSMDADGTKAGFDLEMIAAARAAVDVPVIASGGAGAIGHFAPAVQAGADAVLAASVFHFREL
TIGEVKAAMAAEGITVR

Sequences:

>Translated_257_residues
MSVATRVIACLDVDDGRVVKGVNFENLRDAGDPVELAAAYDAEGVDELTFLDVTASSSGRATMLDVVRRTAEQVFIPLTV
GGGVRSVDDVNVLLRAGADKVGVNTAAIARPELLAELAQRFGSQCIVLSVDARRVRDGDVPTSSGWEVTTHGGRRGTGID
AIEWTSRGAELGVGEILLNSMDADGTKAGFDLEMIAAARAAVDVPVIASGGAGAIGHFAPAVQAGADAVLAASVFHFREL
TIGEVKAAMAAEGITVR
>Mature_256_residues
SVATRVIACLDVDDGRVVKGVNFENLRDAGDPVELAAAYDAEGVDELTFLDVTASSSGRATMLDVVRRTAEQVFIPLTVG
GGVRSVDDVNVLLRAGADKVGVNTAAIARPELLAELAQRFGSQCIVLSVDARRVRDGDVPTSSGWEVTTHGGRRGTGIDA
IEWTSRGAELGVGEILLNSMDADGTKAGFDLEMIAAARAAVDVPVIASGGAGAIGHFAPAVQAGADAVLAASVFHFRELT
IGEVKAAMAAEGITVR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=260, Percent_Identity=44.6153846153846, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI87082028, Length=214, Percent_Identity=28.5046728971963, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319725, Length=310, Percent_Identity=36.1290322580645, Blast_Score=173, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_MYCA9 (B1MBX3)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001703398.1
- ProteinModelPortal:   B1MBX3
- SMR:   B1MBX3
- EnsemblBacteria:   EBMYCT00000008064
- GeneID:   5965173
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_2664c
- GeneTree:   EBGT00050000015857
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- BioCyc:   MABS561007:MAB_2664C-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 26501; Mature: 26370

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: NA

Important sites: ACT_SITE 12-12 ACT_SITE 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVATRVIACLDVDDGRVVKGVNFENLRDAGDPVELAAAYDAEGVDELTFLDVTASSSGR
CCCHHEEEEEEECCCCCEEECCCHHHHHCCCCCEEEEEECCCCCCCCEEEEEEECCCCCC
ATMLDVVRRTAEQVFIPLTVGGGVRSVDDVNVLLRAGADKVGVNTAAIARPELLAELAQR
HHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHH
FGSQCIVLSVDARRVRDGDVPTSSGWEVTTHGGRRGTGIDAIEWTSRGAELGVGEILLNS
HCCEEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEECCCCCCCCHHHHHHHC
MDADGTKAGFDLEMIAAARAAVDVPVIASGGAGAIGHFAPAVQAGADAVLAASVFHFREL
CCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHC
TIGEVKAAMAAEGITVR
CHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SVATRVIACLDVDDGRVVKGVNFENLRDAGDPVELAAAYDAEGVDELTFLDVTASSSGR
CCHHEEEEEEECCCCCEEECCCHHHHHCCCCCEEEEEECCCCCCCCEEEEEEECCCCCC
ATMLDVVRRTAEQVFIPLTVGGGVRSVDDVNVLLRAGADKVGVNTAAIARPELLAELAQR
HHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHH
FGSQCIVLSVDARRVRDGDVPTSSGWEVTTHGGRRGTGIDAIEWTSRGAELGVGEILLNS
HCCEEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEECCCCCCCCHHHHHHHC
MDADGTKAGFDLEMIAAARAAVDVPVIASGGAGAIGHFAPAVQAGADAVLAASVFHFREL
CCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHC
TIGEVKAAMAAEGITVR
CHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA