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The map label for this gene is polA [H]

Identifier: 169629701

GI number: 169629701

Start: 2655155

End: 2657863

Strand: Reverse

Name: polA [H]

Synonym: MAB_2615c

Alternate gene names: 169629701

Gene position: 2657863-2655155 (Counterclockwise)

Preceding gene: 169629702

Following gene: 169629692

Centisome position: 52.45

GC content: 64.05

Gene sequence:

>2709_bases
GTGACTGCGCCTGCGACGAAGACCGGAACCAAACAGCCGACCCTCCTGCTGCTCGACGGTAACTCGCTGGCGTTCCGCGC
GTTCTACGCGCTTCCGGCCGAGAATTTCAAGACGCAGTCGGGCTTGACCACCAACGCGGTATACGGGTTCACCTCGATGT
TGATCAATCTGCTGCGCGATGAGGCGCCCACACACGTGGCGGCCGCCTTCGATGTCTCCCGCAAGACGTTCCGGTCCGAC
CGGTACCCCGAGTACAAGGCCACTCGGTCGGCCACCCCCGACGAGTTCCGCGGACAGATCGACATCACCAAGGAAGTACT
CCAGGCCCTGGGCATCACGGTGCTGGCAGAGGAGGGGTTCGAGGCCGACGACATCATCGCCACCCTGGCCACCCAGGGGC
AGGCCGAGGGTTTCAAGGTGTTCGTGGTCACGGGCGATCGCGACTCGCTGCAGCTCGTCAACGAGGACGTGACGGTGCTC
TACCCGCGCAAGGGTGTCAGCGATCTCACCCGATTCACCCCGGAAGCGGTAGTCGAGAAGTACGGACTCACGCCCTCCCA
GTACCCGGACTTCGCGGCGCTGCGCGGTGACCCGAGCGACAACCTGCCGGGAATTCCTGGAGTTGGGGAGAAGACCGCGT
CCAAGTGGATCCTCGAGTACGGATCGCTCCAAGAGCTGGTTGACAAGGCCGATACCGTGCGCGGAAAGGTGGGGGATTCG
CTGCGCGCCAACCTCGCCTCGGTGATCCTGAACCGCGAACTGACCGATCTGGTGCGGACGGTGCCGCTGCCCTATGTGCC
CGCCCAGTTGGCGCTGGCGCCGTGGGATCGGGACCAGATTCACCGGCTGTTCGACGACCTCGAGTTCCGGGTCCTGCGTG
ACCGGCTGTTCGAAACCCTGGAAGCGGTCGAACCCGAGGTGGACGAGGGCTTCCAGCTGGCCGGCCAGGCGCTGGAACCC
GGCACGGTCGCCGCGTGGCTGGCCGAACACACCGGGGATGGCCGCCGTAGCGGTTTGGCGGTGGTCGGGACCTATACGCC
CTATGACGGCGACGCTACCGCGGTGGCGATCGCGTCCGCCGATGGCGACGGCGCCTATATAGACACCACGACGGTGACTC
CGGACGATGAGGCGGCACTTGCCGAGTGGCTGGCCGATGCGGCCCGTCCCAAGGCATTGCATGAGGCGAAGCAGGCCATA
CACGCTTTGGCCGGCCGCGGGTGGGAGCTCGGCGGAGTGACCTCCGATACCGCGCTGGCTGCGTATCTGGTGCGCCCGGG
GCAGCGCAGCTTCGCACTCGACGACTTGTCGTTGCGGTATCTGCGTCGCGAACTTCGCGCTGAAGATGATGGAGAACAAC
AGCTCTCACTACTGGACGAGGATGGCGCGGGCGATGCGAAGGCAGCTCAAGCCCAGATGTTGCGTGCCCGTGCGGTGATC
GATCTGGCCGACGCGCTCGATGTCGAGCTCGAACGCATCGAATCGGCAGGGCTGCTCACCGACATGGAGCTGCCGGTTCA
GCGGGTGCTGGCCGGTCTGGAATCCGTCGGTATCGCGGTCGACACCGATCATCTGTCTTCGCTGCAGAATCAGTTCGCCA
GCGGCATCCGTGAGGCTGCGGATGCCGCGTACGCCGTCATCGGTAAGCAGATCAACCTGGGCTCACCCAAGCAGCTGCAG
GTGGTGCTGTTCGACGAGTTGGGTATGCCCAAGACCAAGAAGACAAAGACCGGTTACACCACCGACGCGGACGCCCTACA
GACGCTGTTCGATAAGACCGGGCATCCGTTTCTGGAGCATCTGCTCACGCACCGTGATGTCACCAGGCTGAAGGTGACTG
TCGACGGCTTGCTCAAATCTGTTGCGGCCGACGGGCGTATTCACACCACGTTCAACCAGACCATCGCGGCGACTGGTCGG
CTGTCCTCGACCGAACCGAACTTGCAGAACATCCCGGTACGTACCGAGGCCGGGCGGGAGATCCGCGACGGATTCGTGGT
CGGCGCGGGCTACCGCGAGCTGATGACCGTCGACTACAGCCAGATCGAGATGCGCATCATGGCGCACCTGTCGGCCGACG
AGGGCCTCATCGAGGCGTTCAACACCGGTGAGGATTTACACTCGTTCGTGGCCGCCCGTGCCTTCGGTGTGCCCATCGAC
GAGGTCACCGCGGAGTTGCGCCGGCGGGTCAAGGCAATGTCCTACGGCCTGGCGTATGGTCTGTCGGCCTATGGACTGGC
CACACAGCTCAAGATCTCCAATGATGAGGCCAAAGAGCAGATGGACCAATATTTCTCGCGGTTTGGCGGGGTGCGCGACT
ATCTGCGTGCAGTTGTCGATCAGGCCCGCAAGGATGGATACACCTCGACCGTGCTGGGGCGCCGTCGATACCTGCCCGAT
TTGGACAGTGGCGATCGCCTCCGCCGCGAGGCCGCAGAACGCGCGGCTCTGAACGCTCCAATCCAGGGGAGCGCCGCCGA
CATCATCAAGGTTGCGATGATCACCACGGATAAGGCATTGAGGGACAAGGGCTTACGGTCACGAATGCTCCTGCAGGTGC
ACGATGAGCTCCTCTTCGAGGTTGCCGAGGGCGAACGCGAGAGCCTCGAGGCATTGGTACGCAAGACCATGGGCTCGGCG
TATCCACTCGATGTGCCGCTCGAGGTTTCCGTGGGGTACGGCCGCAGCTGGGATGCCGCCGCACACTGA

Upstream 100 bases:

>100_bases
ATCCCTCGGGCCACTACCTGGAGATCCTCACCCGGCCCTACGGGTCAGGGGAAGCGTCGGGGTCGTAGGTCCTGTCGCAC
CCGTTGCCTAGGGTAGGAGT

Downstream 100 bases:

>100_bases
TATTGCGCGCTAGTGCACGTCGGGCGTGTGTACCCGCTCGGGACGCAGCCCCCTGCCGACGGCGATCGGCGGTATGAATT
TGAGCGGCCACCGCCCCACG

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 902; Mature: 901

Protein sequence:

>902_residues
MTAPATKTGTKQPTLLLLDGNSLAFRAFYALPAENFKTQSGLTTNAVYGFTSMLINLLRDEAPTHVAAAFDVSRKTFRSD
RYPEYKATRSATPDEFRGQIDITKEVLQALGITVLAEEGFEADDIIATLATQGQAEGFKVFVVTGDRDSLQLVNEDVTVL
YPRKGVSDLTRFTPEAVVEKYGLTPSQYPDFAALRGDPSDNLPGIPGVGEKTASKWILEYGSLQELVDKADTVRGKVGDS
LRANLASVILNRELTDLVRTVPLPYVPAQLALAPWDRDQIHRLFDDLEFRVLRDRLFETLEAVEPEVDEGFQLAGQALEP
GTVAAWLAEHTGDGRRSGLAVVGTYTPYDGDATAVAIASADGDGAYIDTTTVTPDDEAALAEWLADAARPKALHEAKQAI
HALAGRGWELGGVTSDTALAAYLVRPGQRSFALDDLSLRYLRRELRAEDDGEQQLSLLDEDGAGDAKAAQAQMLRARAVI
DLADALDVELERIESAGLLTDMELPVQRVLAGLESVGIAVDTDHLSSLQNQFASGIREAADAAYAVIGKQINLGSPKQLQ
VVLFDELGMPKTKKTKTGYTTDADALQTLFDKTGHPFLEHLLTHRDVTRLKVTVDGLLKSVAADGRIHTTFNQTIAATGR
LSSTEPNLQNIPVRTEAGREIRDGFVVGAGYRELMTVDYSQIEMRIMAHLSADEGLIEAFNTGEDLHSFVAARAFGVPID
EVTAELRRRVKAMSYGLAYGLSAYGLATQLKISNDEAKEQMDQYFSRFGGVRDYLRAVVDQARKDGYTSTVLGRRRYLPD
LDSGDRLRREAAERAALNAPIQGSAADIIKVAMITTDKALRDKGLRSRMLLQVHDELLFEVAEGERESLEALVRKTMGSA
YPLDVPLEVSVGYGRSWDAAAH

Sequences:

>Translated_902_residues
MTAPATKTGTKQPTLLLLDGNSLAFRAFYALPAENFKTQSGLTTNAVYGFTSMLINLLRDEAPTHVAAAFDVSRKTFRSD
RYPEYKATRSATPDEFRGQIDITKEVLQALGITVLAEEGFEADDIIATLATQGQAEGFKVFVVTGDRDSLQLVNEDVTVL
YPRKGVSDLTRFTPEAVVEKYGLTPSQYPDFAALRGDPSDNLPGIPGVGEKTASKWILEYGSLQELVDKADTVRGKVGDS
LRANLASVILNRELTDLVRTVPLPYVPAQLALAPWDRDQIHRLFDDLEFRVLRDRLFETLEAVEPEVDEGFQLAGQALEP
GTVAAWLAEHTGDGRRSGLAVVGTYTPYDGDATAVAIASADGDGAYIDTTTVTPDDEAALAEWLADAARPKALHEAKQAI
HALAGRGWELGGVTSDTALAAYLVRPGQRSFALDDLSLRYLRRELRAEDDGEQQLSLLDEDGAGDAKAAQAQMLRARAVI
DLADALDVELERIESAGLLTDMELPVQRVLAGLESVGIAVDTDHLSSLQNQFASGIREAADAAYAVIGKQINLGSPKQLQ
VVLFDELGMPKTKKTKTGYTTDADALQTLFDKTGHPFLEHLLTHRDVTRLKVTVDGLLKSVAADGRIHTTFNQTIAATGR
LSSTEPNLQNIPVRTEAGREIRDGFVVGAGYRELMTVDYSQIEMRIMAHLSADEGLIEAFNTGEDLHSFVAARAFGVPID
EVTAELRRRVKAMSYGLAYGLSAYGLATQLKISNDEAKEQMDQYFSRFGGVRDYLRAVVDQARKDGYTSTVLGRRRYLPD
LDSGDRLRREAAERAALNAPIQGSAADIIKVAMITTDKALRDKGLRSRMLLQVHDELLFEVAEGERESLEALVRKTMGSA
YPLDVPLEVSVGYGRSWDAAAH
>Mature_901_residues
TAPATKTGTKQPTLLLLDGNSLAFRAFYALPAENFKTQSGLTTNAVYGFTSMLINLLRDEAPTHVAAAFDVSRKTFRSDR
YPEYKATRSATPDEFRGQIDITKEVLQALGITVLAEEGFEADDIIATLATQGQAEGFKVFVVTGDRDSLQLVNEDVTVLY
PRKGVSDLTRFTPEAVVEKYGLTPSQYPDFAALRGDPSDNLPGIPGVGEKTASKWILEYGSLQELVDKADTVRGKVGDSL
RANLASVILNRELTDLVRTVPLPYVPAQLALAPWDRDQIHRLFDDLEFRVLRDRLFETLEAVEPEVDEGFQLAGQALEPG
TVAAWLAEHTGDGRRSGLAVVGTYTPYDGDATAVAIASADGDGAYIDTTTVTPDDEAALAEWLADAARPKALHEAKQAIH
ALAGRGWELGGVTSDTALAAYLVRPGQRSFALDDLSLRYLRRELRAEDDGEQQLSLLDEDGAGDAKAAQAQMLRARAVID
LADALDVELERIESAGLLTDMELPVQRVLAGLESVGIAVDTDHLSSLQNQFASGIREAADAAYAVIGKQINLGSPKQLQV
VLFDELGMPKTKKTKTGYTTDADALQTLFDKTGHPFLEHLLTHRDVTRLKVTVDGLLKSVAADGRIHTTFNQTIAATGRL
SSTEPNLQNIPVRTEAGREIRDGFVVGAGYRELMTVDYSQIEMRIMAHLSADEGLIEAFNTGEDLHSFVAARAFGVPIDE
VTAELRRRVKAMSYGLAYGLSAYGLATQLKISNDEAKEQMDQYFSRFGGVRDYLRAVVDQARKDGYTSTVLGRRRYLPDL
DSGDRLRREAAERAALNAPIQGSAADIIKVAMITTDKALRDKGLRSRMLLQVHDELLFEVAEGERESLEALVRKTMGSAY
PLDVPLEVSVGYGRSWDAAAH

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=523, Percent_Identity=31.1663479923518, Blast_Score=227, Evalue=4e-59,
Organism=Homo sapiens, GI139394648, Length=616, Percent_Identity=27.2727272727273, Blast_Score=203, Evalue=7e-52,
Organism=Escherichia coli, GI1790294, Length=510, Percent_Identity=40.7843137254902, Blast_Score=338, Evalue=8e-94,
Organism=Escherichia coli, GI226510970, Length=208, Percent_Identity=28.8461538461538, Blast_Score=99, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI86563272, Length=422, Percent_Identity=28.6729857819905, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI17933644, Length=449, Percent_Identity=33.630289532294, Blast_Score=224, Evalue=2e-58,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR003583
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 98327; Mature: 98196

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPATKTGTKQPTLLLLDGNSLAFRAFYALPAENFKTQSGLTTNAVYGFTSMLINLLRD
CCCCCCCCCCCCCEEEEEECCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
EAPTHVAAAFDVSRKTFRSDRYPEYKATRSATPDEFRGQIDITKEVLQALGITVLAEEGF
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHCCEEEEECCCC
EADDIIATLATQGQAEGFKVFVVTGDRDSLQLVNEDVTVLYPRKGVSDLTRFTPEAVVEK
CHHHHHHHHHCCCCCCCEEEEEEECCCCCHHEECCCEEEEECCCCHHHHHHCCHHHHHHH
YGLTPSQYPDFAALRGDPSDNLPGIPGVGEKTASKWILEYGSLQELVDKADTVRGKVGDS
HCCCCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCH
LRANLASVILNRELTDLVRTVPLPYVPAQLALAPWDRDQIHRLFDDLEFRVLRDRLFETL
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
EAVEPEVDEGFQLAGQALEPGTVAAWLAEHTGDGRRSGLAVVGTYTPYDGDATAVAIASA
HHHCCCCCCCHHHHCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEEEEEC
DGDGAYIDTTTVTPDDEAALAEWLADAARPKALHEAKQAIHALAGRGWELGGVTSDTALA
CCCCCEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEECCCCCCHHHHH
AYLVRPGQRSFALDDLSLRYLRRELRAEDDGEQQLSLLDEDGAGDAKAAQAQMLRARAVI
HHHCCCCCCCEEHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
DLADALDVELERIESAGLLTDMELPVQRVLAGLESVGIAVDTDHLSSLQNQFASGIREAA
HHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHH
DAAYAVIGKQINLGSPKQLQVVLFDELGMPKTKKTKTGYTTDADALQTLFDKTGHPFLEH
HHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHH
LLTHRDVTRLKVTVDGLLKSVAADGRIHTTFNQTIAATGRLSSTEPNLQNIPVRTEAGRE
HHHHCCHHEEEEEHHHHHHHHHCCCEEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCH
IRDGFVVGAGYRELMTVDYSQIEMRIMAHLSADEGLIEAFNTGEDLHSFVAARAFGVPID
HHCCEEECCCHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHCCCCHH
EVTAELRRRVKAMSYGLAYGLSAYGLATQLKISNDEAKEQMDQYFSRFGGVRDYLRAVVD
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHH
QARKDGYTSTVLGRRRYLPDLDSGDRLRREAAERAALNAPIQGSAADIIKVAMITTDKAL
HHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHEEHHHCCCHHH
RDKGLRSRMLLQVHDELLFEVAEGERESLEALVRKTMGSAYPLDVPLEVSVGYGRSWDAA
HHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCC
AH
CC
>Mature Secondary Structure 
TAPATKTGTKQPTLLLLDGNSLAFRAFYALPAENFKTQSGLTTNAVYGFTSMLINLLRD
CCCCCCCCCCCCEEEEEECCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
EAPTHVAAAFDVSRKTFRSDRYPEYKATRSATPDEFRGQIDITKEVLQALGITVLAEEGF
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHCCEEEEECCCC
EADDIIATLATQGQAEGFKVFVVTGDRDSLQLVNEDVTVLYPRKGVSDLTRFTPEAVVEK
CHHHHHHHHHCCCCCCCEEEEEEECCCCCHHEECCCEEEEECCCCHHHHHHCCHHHHHHH
YGLTPSQYPDFAALRGDPSDNLPGIPGVGEKTASKWILEYGSLQELVDKADTVRGKVGDS
HCCCCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCH
LRANLASVILNRELTDLVRTVPLPYVPAQLALAPWDRDQIHRLFDDLEFRVLRDRLFETL
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
EAVEPEVDEGFQLAGQALEPGTVAAWLAEHTGDGRRSGLAVVGTYTPYDGDATAVAIASA
HHHCCCCCCCHHHHCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEEEEEC
DGDGAYIDTTTVTPDDEAALAEWLADAARPKALHEAKQAIHALAGRGWELGGVTSDTALA
CCCCCEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEECCCCCCHHHHH
AYLVRPGQRSFALDDLSLRYLRRELRAEDDGEQQLSLLDEDGAGDAKAAQAQMLRARAVI
HHHCCCCCCCEEHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
DLADALDVELERIESAGLLTDMELPVQRVLAGLESVGIAVDTDHLSSLQNQFASGIREAA
HHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHH
DAAYAVIGKQINLGSPKQLQVVLFDELGMPKTKKTKTGYTTDADALQTLFDKTGHPFLEH
HHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHH
LLTHRDVTRLKVTVDGLLKSVAADGRIHTTFNQTIAATGRLSSTEPNLQNIPVRTEAGRE
HHHHCCHHEEEEEHHHHHHHHHCCCEEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCH
IRDGFVVGAGYRELMTVDYSQIEMRIMAHLSADEGLIEAFNTGEDLHSFVAARAFGVPID
HHCCEEECCCHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHCCCCHH
EVTAELRRRVKAMSYGLAYGLSAYGLATQLKISNDEAKEQMDQYFSRFGGVRDYLRAVVD
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHH
QARKDGYTSTVLGRRRYLPDLDSGDRLRREAAERAALNAPIQGSAADIIKVAMITTDKAL
HHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHEEHHHCCCHHH
RDKGLRSRMLLQVHDELLFEVAEGERESLEALVRKTMGSAYPLDVPLEVSVGYGRSWDAA
HHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCC
AH
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]