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The map label for this gene is cysJ [H]

Identifier: 169629578

GI number: 169629578

Start: 2546370

End: 2547986

Strand: Direct

Name: cysJ [H]

Synonym: MAB_2492

Alternate gene names: 169629578

Gene position: 2546370-2547986 (Clockwise)

Preceding gene: 169629576

Following gene: 169629581

Centisome position: 50.25

GC content: 63.51

Gene sequence:

>1617_bases
GTGACCGCCCAGCCGGATTTTTCGTTGATCGTCGGCTACGGCACCGATATGGGCAACGCCGAGGACGCCGCCATGTCGTT
CTGCGAGGCTCTGGAAGAGGCCACCGGCATCAAATCCGAGGCGGTCGAACTCAACCAGGTCGATATCGCGAACTTGCAGT
CGGCGTCACACCTCGTCGTGGTGGTGTCCACCTTCGGCGACGGCGAATTTCCCGATAATGCGCTGTTGTTCTGGGAGGCG
CTGAGTGCTGAAGGCGCCGAGCGGCTTGCGCACCTGCAATTCGCGGTCTTGGCATTGGGCGACAGCAGCTACGAATTCTT
CTGCAATGCGGGGCTGCTGTTGGACAAGCGCCTGGAGGAGCTTGGCGCGAGTCGGCTCACCGAACGTACCGATGTCGACG
GACCGTACCTGCAGCCCTCGAAGGAATGGACCACCGATCTGGTGAAGCTGCTGGCCGCCGGCCCTATTGGGTCCACGGAA
TCCGCCGTCGTCACCGAAACGGCAACACCCGCCAAACGCGAACGCCACGAAACCGTCGAGGCACGCCTTGTTGTCAACCG
ATTGCTCACCACGGCGGAATCCGACAAGGAGGTGCGTCACTATGAGGTGGACCTCGCGGGGACCGGAATCTCCTATGAAG
CCGGCGATTCCATCGCCGTGCACGCGCCCAATGACCCCGCATTGGTCGAGGCGATCCTTGCCGAGTTGGGCGTGCCGGCC
GCGCATCGGCTAGCCGGCCACGACCTGCCCCTCGGTGAGCTGTTATCCGACCACCTGGAGATCCGGACTCCCTCGCGAAC
GCTTCGGGCGTTGGTCGCATCCCGCACCGACGACGCGGATGCGGTCGCCGCGCTCAGCGGTGACGGCACGCCGGTACCGG
GCTCCTGGCTATACGGTAAGGATGTGTTGGACCTGGTCAGGCTGGGAAAGCTGGCCGTTGAGGAACTGATCGACAATCTG
CGCCCCCTGCAGTTCCGCGATTACTCCATCGCCTCAAGCCCTGTCGTGTATCCCGATACGGTTCATCTGACCGTGGCGAC
CATCCGCTACTCCAATGCCGATCGCACGTATGGCGGTGTGGCGTCGACATTCTTGGCTGATCGCGGGCAGGCGGTCCGGG
TGCATCTGCGCCCAAACCACGCCTTCCGGCTGCCCGGGGGCCATGTCCCGATCATCATGATCGGGCCGGGGACGGGGATT
GCGCCGTTTCGCGGCTTTCTGCAAGAGCGCCAGGCGGCCGGTTCCACGGGGCGTTCCTGGCTGTTCTTCGGTGACCGGCA
TCGCGCCGCGAGTTTCCTCTACGGCGAGGAGCTGCAGGGGTTCGTGGCCTCGGGTGTTCTGACTCGACTTGACCTTGCCT
TCTCCCGTGACGGGGCCAACGGTGAGCCCAAACAGTATGTCCAACATCGTATGTCGGAGAATGCGGCCGAGCTGTGGGCC
TGGCTCCAGGACGGGGCTTATGTGTATGTGTGCGGCGACGCCGAGCACATGGCAAAGGACGTCGATGCGACACTGCACGA
GATCGTGGCTCGCTGCGGTGGTATGGACGCCGCCGGCGCACACGCTTATGTCAATGAACTCATCAAGAACCACCGGTACG
TGCGCGACGTCTACTAG

Upstream 100 bases:

>100_bases
AGGCAGGGAAAGAGGGGAGGCCCCCACAGTGCCCTGTGTCAAAGTAAGGTCGCCCTCACCCAGCGCTGGGATCAGCCCCG
CGCGCGAGTGAGGGAGCGCT

Downstream 100 bases:

>100_bases
AGCCGACGCGCGCACCGGCGGAGCGCACCTACGAATGCACCTGAATCTCGCGGAGCGCCAAGATCGCGTCGATCTCGCCG
TGAAGGAGGTGATGGTTGAA

Product: sulfite reductase

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MTAQPDFSLIVGYGTDMGNAEDAAMSFCEALEEATGIKSEAVELNQVDIANLQSASHLVVVVSTFGDGEFPDNALLFWEA
LSAEGAERLAHLQFAVLALGDSSYEFFCNAGLLLDKRLEELGASRLTERTDVDGPYLQPSKEWTTDLVKLLAAGPIGSTE
SAVVTETATPAKRERHETVEARLVVNRLLTTAESDKEVRHYEVDLAGTGISYEAGDSIAVHAPNDPALVEAILAELGVPA
AHRLAGHDLPLGELLSDHLEIRTPSRTLRALVASRTDDADAVAALSGDGTPVPGSWLYGKDVLDLVRLGKLAVEELIDNL
RPLQFRDYSIASSPVVYPDTVHLTVATIRYSNADRTYGGVASTFLADRGQAVRVHLRPNHAFRLPGGHVPIIMIGPGTGI
APFRGFLQERQAAGSTGRSWLFFGDRHRAASFLYGEELQGFVASGVLTRLDLAFSRDGANGEPKQYVQHRMSENAAELWA
WLQDGAYVYVCGDAEHMAKDVDATLHEIVARCGGMDAAGAHAYVNELIKNHRYVRDVY

Sequences:

>Translated_538_residues
MTAQPDFSLIVGYGTDMGNAEDAAMSFCEALEEATGIKSEAVELNQVDIANLQSASHLVVVVSTFGDGEFPDNALLFWEA
LSAEGAERLAHLQFAVLALGDSSYEFFCNAGLLLDKRLEELGASRLTERTDVDGPYLQPSKEWTTDLVKLLAAGPIGSTE
SAVVTETATPAKRERHETVEARLVVNRLLTTAESDKEVRHYEVDLAGTGISYEAGDSIAVHAPNDPALVEAILAELGVPA
AHRLAGHDLPLGELLSDHLEIRTPSRTLRALVASRTDDADAVAALSGDGTPVPGSWLYGKDVLDLVRLGKLAVEELIDNL
RPLQFRDYSIASSPVVYPDTVHLTVATIRYSNADRTYGGVASTFLADRGQAVRVHLRPNHAFRLPGGHVPIIMIGPGTGI
APFRGFLQERQAAGSTGRSWLFFGDRHRAASFLYGEELQGFVASGVLTRLDLAFSRDGANGEPKQYVQHRMSENAAELWA
WLQDGAYVYVCGDAEHMAKDVDATLHEIVARCGGMDAAGAHAYVNELIKNHRYVRDVY
>Mature_537_residues
TAQPDFSLIVGYGTDMGNAEDAAMSFCEALEEATGIKSEAVELNQVDIANLQSASHLVVVVSTFGDGEFPDNALLFWEAL
SAEGAERLAHLQFAVLALGDSSYEFFCNAGLLLDKRLEELGASRLTERTDVDGPYLQPSKEWTTDLVKLLAAGPIGSTES
AVVTETATPAKRERHETVEARLVVNRLLTTAESDKEVRHYEVDLAGTGISYEAGDSIAVHAPNDPALVEAILAELGVPAA
HRLAGHDLPLGELLSDHLEIRTPSRTLRALVASRTDDADAVAALSGDGTPVPGSWLYGKDVLDLVRLGKLAVEELIDNLR
PLQFRDYSIASSPVVYPDTVHLTVATIRYSNADRTYGGVASTFLADRGQAVRVHLRPNHAFRLPGGHVPIIMIGPGTGIA
PFRGFLQERQAAGSTGRSWLFFGDRHRAASFLYGEELQGFVASGVLTRLDLAFSRDGANGEPKQYVQHRMSENAAELWAW
LQDGAYVYVCGDAEHMAKDVDATLHEIVARCGGMDAAGAHAYVNELIKNHRYVRDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI127139033, Length=610, Percent_Identity=31.6393442622951, Blast_Score=238, Evalue=8e-63,
Organism=Homo sapiens, GI7657393, Length=602, Percent_Identity=27.4086378737542, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI221316705, Length=608, Percent_Identity=27.3026315789474, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI10835173, Length=644, Percent_Identity=26.5527950310559, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI169790956, Length=418, Percent_Identity=30.8612440191388, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI169790958, Length=418, Percent_Identity=30.8612440191388, Blast_Score=162, Evalue=8e-40,
Organism=Homo sapiens, GI221316709, Length=602, Percent_Identity=27.2425249169435, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI40254422, Length=643, Percent_Identity=27.6827371695179, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI221316707, Length=524, Percent_Identity=28.4351145038168, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI24041029, Length=606, Percent_Identity=28.0528052805281, Blast_Score=145, Evalue=1e-34,
Organism=Escherichia coli, GI1789123, Length=547, Percent_Identity=40.2193784277879, Blast_Score=357, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI17554134, Length=596, Percent_Identity=31.3758389261745, Blast_Score=235, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI17566446, Length=579, Percent_Identity=25.73402417962, Blast_Score=141, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI17531441, Length=393, Percent_Identity=27.4809160305344, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321143, Length=388, Percent_Identity=29.3814432989691, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6321832, Length=636, Percent_Identity=25.1572327044025, Blast_Score=150, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6325305, Length=629, Percent_Identity=23.5294117647059, Blast_Score=124, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6322597, Length=151, Percent_Identity=33.7748344370861, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI17137192, Length=616, Percent_Identity=28.8961038961039, Blast_Score=213, Evalue=3e-55,
Organism=Drosophila melanogaster, GI24582192, Length=528, Percent_Identity=28.9772727272727, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI78706872, Length=675, Percent_Identity=26.2222222222222, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24583543, Length=675, Percent_Identity=26.2222222222222, Blast_Score=162, Evalue=5e-40,
Organism=Drosophila melanogaster, GI78706876, Length=675, Percent_Identity=26.2222222222222, Blast_Score=162, Evalue=5e-40,
Organism=Drosophila melanogaster, GI24660907, Length=598, Percent_Identity=27.0903010033445, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24660903, Length=598, Percent_Identity=27.0903010033445, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24647438, Length=397, Percent_Identity=24.1813602015113, Blast_Score=76, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 58208; Mature: 58077

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQPDFSLIVGYGTDMGNAEDAAMSFCEALEEATGIKSEAVELNQVDIANLQSASHLVV
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHCEEECCCCHHHCCCCCEEEE
VVSTFGDGEFPDNALLFWEALSAEGAERLAHLQFAVLALGDSSYEFFCNAGLLLDKRLEE
EEEECCCCCCCCCCEEEEEHHCHHHHHHHHHHHEEEEEECCCCCEEEECCCHHHHHHHHH
LGASRLTERTDVDGPYLQPSKEWTTDLVKLLAAGPIGSTESAVVTETATPAKRERHETVE
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHH
ARLVVNRLLTTAESDKEVRHYEVDLAGTGISYEAGDSIAVHAPNDPALVEAILAELGVPA
HHHHHHHHHHHCCCCCCCEEEEEEEECCCCCEECCCEEEEECCCCHHHHHHHHHHHCCCH
AHRLAGHDLPLGELLSDHLEIRTPSRTLRALVASRTDDADAVAALSGDGTPVPGSWLYGK
HHHHCCCCCCHHHHHHHHHEECCCHHHHHHHHHCCCCCCHHEEEECCCCCCCCCCCCCCH
DVLDLVRLGKLAVEELIDNLRPLQFRDYSIASSPVVYPDTVHLTVATIRYSNADRTYGGV
HHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEECCCEEEEEEEEEEECCCCCCCCHH
ASTFLADRGQAVRVHLRPNHAFRLPGGHVPIIMIGPGTGIAPFRGFLQERQAAGSTGRSW
HHHHHHCCCCEEEEEECCCCEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEE
LFFGDRHRAASFLYGEELQGFVASGVLTRLDLAFSRDGANGEPKQYVQHRMSENAAELWA
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHCCCHHHHHH
WLQDGAYVYVCGDAEHMAKDVDATLHEIVARCGGMDAAGAHAYVNELIKNHRYVRDVY
HHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHCCC
>Mature Secondary Structure 
TAQPDFSLIVGYGTDMGNAEDAAMSFCEALEEATGIKSEAVELNQVDIANLQSASHLVV
CCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHCEEECCCCHHHCCCCCEEEE
VVSTFGDGEFPDNALLFWEALSAEGAERLAHLQFAVLALGDSSYEFFCNAGLLLDKRLEE
EEEECCCCCCCCCCEEEEEHHCHHHHHHHHHHHEEEEEECCCCCEEEECCCHHHHHHHHH
LGASRLTERTDVDGPYLQPSKEWTTDLVKLLAAGPIGSTESAVVTETATPAKRERHETVE
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHH
ARLVVNRLLTTAESDKEVRHYEVDLAGTGISYEAGDSIAVHAPNDPALVEAILAELGVPA
HHHHHHHHHHHCCCCCCCEEEEEEEECCCCCEECCCEEEEECCCCHHHHHHHHHHHCCCH
AHRLAGHDLPLGELLSDHLEIRTPSRTLRALVASRTDDADAVAALSGDGTPVPGSWLYGK
HHHHCCCCCCHHHHHHHHHEECCCHHHHHHHHHCCCCCCHHEEEECCCCCCCCCCCCCCH
DVLDLVRLGKLAVEELIDNLRPLQFRDYSIASSPVVYPDTVHLTVATIRYSNADRTYGGV
HHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEECCCEEEEEEEEEEECCCCCCCCHH
ASTFLADRGQAVRVHLRPNHAFRLPGGHVPIIMIGPGTGIAPFRGFLQERQAAGSTGRSW
HHHHHHCCCCEEEEEECCCCEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEE
LFFGDRHRAASFLYGEELQGFVASGVLTRLDLAFSRDGANGEPKQYVQHRMSENAAELWA
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHCCCHHHHHH
WLQDGAYVYVCGDAEHMAKDVDATLHEIVARCGGMDAAGAHAYVNELIKNHRYVRDVY
HHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]