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The map label for this gene is shiA [H]

Identifier: 169629567

GI number: 169629567

Start: 2531986

End: 2533323

Strand: Direct

Name: shiA [H]

Synonym: MAB_2481

Alternate gene names: 169629567

Gene position: 2531986-2533323 (Clockwise)

Preceding gene: 169629566

Following gene: 169629568

Centisome position: 49.97

GC content: 65.62

Gene sequence:

>1338_bases
GTGACTACCACCAGTGCGGTACCTGCTGTCCATGCGGGCGGTCCGGCTGACGGGGACGCGCCCGCGGCGCGCACGGTCGC
GCTGAGTTGCATGGTCGGCACCACGATCGAGTGGTACGACTTCTATCTCTACGCGACGGCCGCGGCGTTGGTTCTCAAGC
CGCTGTTCTTCCCGAGCGTCTCGCCGACGGCAGGCACGCTGGCCTCGTTTGCCACCTATGCGGCCGGCTTCGGCGCGCGC
CCGGTGGGAGCCGTGATCGCCGGGCACTTGGGGGACAGGGTGGGGCGCAAGGCCGTCCTTGTCGGTGCGTTGCTGCTCAT
GGGGGTGGCGACCGCGGTGATCGGTGCACTGCCCACCTACTCCGAGGCGGGTGTGCTGGCGCCTGCGGCGCTGGCGACGC
TTCGGGTCCTGCAGGGGCTTGCCGCCGGGGCCGAGTGGGGCGGGGCCGCTCTGCTGGCGGTTGAACATGCCCCGGTGGGG
CGGCGAGGTCTATTCGGTAGCTTCACGCAATTGGGGTCACCCGCGGGAATGCTCTTGGCCACCGGAGTGTTCGGGCTCAC
CCGAGCCCTGACCGGGCCGGAGGCATTTCTAGCCTTCGGCTGGCGGATCCCCTTTCTGAGCAGCATCGTCCTGGTGGTCG
TTGGTTTGGCCATCCGGTTACGGTTGAGGGACTCGGTGGTGTTCCGGAAGGTACTGGAACGCGGGGAGGCGGCCCGGCTT
CCGGTGGTGGAAGTGCTACGCACTCAATCTCGTAATGTGCTGCTTACCACGGGCTTACGCCTATCTCAGATCGCTCTGTT
CGTGCTCCTGACGACGTTCTCGCTGACCTACCTGCAAGGTTCTTTCGGCGGGGACAGCCAGATCGGGTTGACTGCCGTCC
TGATCTCCTCGGCGTTGGGGATCTTGAGCACTCCGGTGTGGTCTGCGCTCTCGGACAGGGTGGGGCGCCGGCCTCTCTAT
CTGTTCGGATCGGCCGCTGGCCCACTGGCCCTGGGGCTCTTTTTTCTGGCCGCGGGCAGTGGCTCGAAGGTGCTGGTGGT
GCTGTCAATTGTGTTCGGTATCAACGTTGTTCATGATGCCATGTACGGTCCACAGGCGGCCTGGTTCGCCGAGCTCTTCG
ATACCAGGGTGCGATACAGCGGCGCCTCGCTGGGTTATCAGATCGGCGCGGTACTGTCCGGTGGATTCGCTCCGTTGATC
GCCGCGGCGCTGCTGGCGGCGAATCATGGCGATCCGCGGCTCATCGTGTGCTACTACCTGGCGCTATCGGTGCTCACCTT
CGGCGCGGCATGTGTGGCACGCGAAACTTACCGGGACCAGATAGGAGATTCCCTGTGA

Upstream 100 bases:

>100_bases
ACATGCGTGCCGACCGCTTCTGACTGTGACGCTTCGGTGCTGAAATCCTGCCGAGTGCAAAGGTTCCGGTCGACCACCCG
CTGATACACCGTGGTGTCGC

Downstream 100 bases:

>100_bases
GCAGGATCTATCTGAATGCCTTCGACATGGCATGTGTCGGGCATCAGTCGCCCGGCCTGTGGCGTCATCCGGATGATCAG
GGGCATCGCTACAAAAGCCT

Product: putative integral membrane transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MTTTSAVPAVHAGGPADGDAPAARTVALSCMVGTTIEWYDFYLYATAAALVLKPLFFPSVSPTAGTLASFATYAAGFGAR
PVGAVIAGHLGDRVGRKAVLVGALLLMGVATAVIGALPTYSEAGVLAPAALATLRVLQGLAAGAEWGGAALLAVEHAPVG
RRGLFGSFTQLGSPAGMLLATGVFGLTRALTGPEAFLAFGWRIPFLSSIVLVVVGLAIRLRLRDSVVFRKVLERGEAARL
PVVEVLRTQSRNVLLTTGLRLSQIALFVLLTTFSLTYLQGSFGGDSQIGLTAVLISSALGILSTPVWSALSDRVGRRPLY
LFGSAAGPLALGLFFLAAGSGSKVLVVLSIVFGINVVHDAMYGPQAAWFAELFDTRVRYSGASLGYQIGAVLSGGFAPLI
AAALLAANHGDPRLIVCYYLALSVLTFGAACVARETYRDQIGDSL

Sequences:

>Translated_445_residues
MTTTSAVPAVHAGGPADGDAPAARTVALSCMVGTTIEWYDFYLYATAAALVLKPLFFPSVSPTAGTLASFATYAAGFGAR
PVGAVIAGHLGDRVGRKAVLVGALLLMGVATAVIGALPTYSEAGVLAPAALATLRVLQGLAAGAEWGGAALLAVEHAPVG
RRGLFGSFTQLGSPAGMLLATGVFGLTRALTGPEAFLAFGWRIPFLSSIVLVVVGLAIRLRLRDSVVFRKVLERGEAARL
PVVEVLRTQSRNVLLTTGLRLSQIALFVLLTTFSLTYLQGSFGGDSQIGLTAVLISSALGILSTPVWSALSDRVGRRPLY
LFGSAAGPLALGLFFLAAGSGSKVLVVLSIVFGINVVHDAMYGPQAAWFAELFDTRVRYSGASLGYQIGAVLSGGFAPLI
AAALLAANHGDPRLIVCYYLALSVLTFGAACVARETYRDQIGDSL
>Mature_444_residues
TTTSAVPAVHAGGPADGDAPAARTVALSCMVGTTIEWYDFYLYATAAALVLKPLFFPSVSPTAGTLASFATYAAGFGARP
VGAVIAGHLGDRVGRKAVLVGALLLMGVATAVIGALPTYSEAGVLAPAALATLRVLQGLAAGAEWGGAALLAVEHAPVGR
RGLFGSFTQLGSPAGMLLATGVFGLTRALTGPEAFLAFGWRIPFLSSIVLVVVGLAIRLRLRDSVVFRKVLERGEAARLP
VVEVLRTQSRNVLLTTGLRLSQIALFVLLTTFSLTYLQGSFGGDSQIGLTAVLISSALGILSTPVWSALSDRVGRRPLYL
FGSAAGPLALGLFFLAAGSGSKVLVVLSIVFGINVVHDAMYGPQAAWFAELFDTRVRYSGASLGYQIGAVLSGGFAPLIA
AALLAANHGDPRLIVCYYLALSVLTFGAACVARETYRDQIGDSL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=418, Percent_Identity=41.3875598086124, Blast_Score=304, Evalue=6e-84,
Organism=Escherichia coli, GI1789941, Length=422, Percent_Identity=40.7582938388626, Blast_Score=245, Evalue=5e-66,
Organism=Escherichia coli, GI1790550, Length=417, Percent_Identity=30.6954436450839, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1788942, Length=380, Percent_Identity=31.3157894736842, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI87082404, Length=403, Percent_Identity=23.8213399503722, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45930; Mature: 45799

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTSAVPAVHAGGPADGDAPAARTVALSCMVGTTIEWYDFYLYATAAALVLKPLFFPSV
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
SPTAGTLASFATYAAGFGARPVGAVIAGHLGDRVGRKAVLVGALLLMGVATAVIGALPTY
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SEAGVLAPAALATLRVLQGLAAGAEWGGAALLAVEHAPVGRRGLFGSFTQLGSPAGMLLA
CCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCHHHCCCCCHHHHH
TGVFGLTRALTGPEAFLAFGWRIPFLSSIVLVVVGLAIRLRLRDSVVFRKVLERGEAARL
HHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PVVEVLRTQSRNVLLTTGLRLSQIALFVLLTTFSLTYLQGSFGGDSQIGLTAVLISSALG
HHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
ILSTPVWSALSDRVGRRPLYLFGSAAGPLALGLFFLAAGSGSKVLVVLSIVFGINVVHDA
HHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
MYGPQAAWFAELFDTRVRYSGASLGYQIGAVLSGGFAPLIAAALLAANHGDPRLIVCYYL
HCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHH
ALSVLTFGAACVARETYRDQIGDSL
HHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TTTSAVPAVHAGGPADGDAPAARTVALSCMVGTTIEWYDFYLYATAAALVLKPLFFPSV
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
SPTAGTLASFATYAAGFGARPVGAVIAGHLGDRVGRKAVLVGALLLMGVATAVIGALPTY
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SEAGVLAPAALATLRVLQGLAAGAEWGGAALLAVEHAPVGRRGLFGSFTQLGSPAGMLLA
CCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCHHHCCCCCHHHHH
TGVFGLTRALTGPEAFLAFGWRIPFLSSIVLVVVGLAIRLRLRDSVVFRKVLERGEAARL
HHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PVVEVLRTQSRNVLLTTGLRLSQIALFVLLTTFSLTYLQGSFGGDSQIGLTAVLISSALG
HHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
ILSTPVWSALSDRVGRRPLYLFGSAAGPLALGLFFLAAGSGSKVLVVLSIVFGINVVHDA
HHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
MYGPQAAWFAELFDTRVRYSGASLGYQIGAVLSGGFAPLIAAALLAANHGDPRLIVCYYL
HCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHH
ALSVLTFGAACVARETYRDQIGDSL
HHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]