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The map label for this gene is aphE [H]

Identifier: 169629471

GI number: 169629471

Start: 2441989

End: 2442756

Strand: Direct

Name: aphE [H]

Synonym: MAB_2385

Alternate gene names: 169629471

Gene position: 2441989-2442756 (Clockwise)

Preceding gene: 169629470

Following gene: 169629472

Centisome position: 48.19

GC content: 67.71

Gene sequence:

>768_bases
TTGATCGATCTGTCGGCCTGGGAGCCGATCGTGACGGGGGAGTCCGGTGCCTGCGTGTACCGCTCGCCCGACGGTTCTCG
CTATGCGAAAAAAGGCGGTGCCGATCTTGCGGGCGAGCGGGACCGCATCGAATGGCTTAGTGCTCAACGTATTCCGGGCC
CATCGGTGCTCGAATGGGCGACCACGTCGGACGGTCCGCTGCTGATCACCTCGGCGGTACCGGGCGTACCCGCCGACCAG
CTCGACTCCGAGGATTTGGGCAGGGCATGGTCTGGGATCGCCGCAGCGGTGCGGGACCTGCACGCGCTGCCGACAGCGGG
CTGTCCGTTTTCGCGAGGGCTGGCCGCCATGGCAACGTTGGCCCGCGACGTCGTGGCCCGCGACGCCGTGAACCCGGATT
TTCTGACCGACGAAGACCGGGAGAGGCCGGCGACCGAGCTGCTATGCCGTGTTGTGGCCGAACTGCCTCGGCGCCTGGCT
CAGGAGCCCGACGACCTGGTGGTGTGCCATGGGGACTTGTGCCTGCCGAATATCGTCATCGCGCCCGGAGACTTCTCAGT
GGCGGGCTTCATCGATCTCGGCCGGCTCGGGTTGGCCGACCGGCACGCCGATCTGGCCTTGCTCTTTGCCAATGCCCGGG
AGACCTGGCCCGGGGACGGCTGGGTCACGGCCGCCGCCCACGAGCGGTTCCTGAACCGGTACGGAGCGTCTGCCGACCCC
GAACGGTTGGCGTTCTACCTGCGGTTGGATCCGCTGACCTGGGGATAG

Upstream 100 bases:

>100_bases
TCATCGCCAACTATGTGGCCCATGAGGTCGACAACCCGAAGGCCATGGCGGCGCGCACGATCGACGTCGCACATCGGCTC
GACGAGGCGTGGACGGGCGT

Downstream 100 bases:

>100_bases
TGATTAAGCATTGACTTATATAAGCAGTCCCTTTTAATATCGGTGACGTGCACGCCTTCGACATACTCGGAGACCCGGTG
CGCCGGCGCATCCTGGAGCT

Product: streptomycin phosphotransferase

Products: NA

Alternate protein names: Streptomycin 3''-phosphotransferase; Streptomycin 6-kinase; Streptomycin 6-phosphotransferase [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MIDLSAWEPIVTGESGACVYRSPDGSRYAKKGGADLAGERDRIEWLSAQRIPGPSVLEWATTSDGPLLITSAVPGVPADQ
LDSEDLGRAWSGIAAAVRDLHALPTAGCPFSRGLAAMATLARDVVARDAVNPDFLTDEDRERPATELLCRVVAELPRRLA
QEPDDLVVCHGDLCLPNIVIAPGDFSVAGFIDLGRLGLADRHADLALLFANARETWPGDGWVTAAAHERFLNRYGASADP
ERLAFYLRLDPLTWG

Sequences:

>Translated_255_residues
MIDLSAWEPIVTGESGACVYRSPDGSRYAKKGGADLAGERDRIEWLSAQRIPGPSVLEWATTSDGPLLITSAVPGVPADQ
LDSEDLGRAWSGIAAAVRDLHALPTAGCPFSRGLAAMATLARDVVARDAVNPDFLTDEDRERPATELLCRVVAELPRRLA
QEPDDLVVCHGDLCLPNIVIAPGDFSVAGFIDLGRLGLADRHADLALLFANARETWPGDGWVTAAAHERFLNRYGASADP
ERLAFYLRLDPLTWG
>Mature_255_residues
MIDLSAWEPIVTGESGACVYRSPDGSRYAKKGGADLAGERDRIEWLSAQRIPGPSVLEWATTSDGPLLITSAVPGVPADQ
LDSEDLGRAWSGIAAAVRDLHALPTAGCPFSRGLAAMATLARDVVARDAVNPDFLTDEDRERPATELLCRVVAELPRRLA
QEPDDLVVCHGDLCLPNIVIAPGDFSVAGFIDLGRLGLADRHADLALLFANARETWPGDGWVTAAAHERFLNRYGASADP
ERLAFYLRLDPLTWG

Specific function: The aminoglycoside phosphotransferases achieve inactivation of their antibiotic substrates by phosphorylation [H]

COG id: COG3231

COG function: function code J; Aminoglycoside phosphotransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aminoglycoside phosphotransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002575
- InterPro:   IPR011009 [H]

Pfam domain/function: PF01636 APH [H]

EC number: =2.7.1.87 [H]

Molecular weight: Translated: 27424; Mature: 27424

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS00109 PROTEIN_KINASE_TYR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLSAWEPIVTGESGACVYRSPDGSRYAKKGGADLAGERDRIEWLSAQRIPGPSVLEWA
CCCCCCCCCEEECCCCEEEEECCCCCHHHHCCCCCCCCCCHHHHHHHHCCCCCCCEEEEE
TTSDGPLLITSAVPGVPADQLDSEDLGRAWSGIAAAVRDLHALPTAGCPFSRGLAAMATL
ECCCCCEEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
ARDVVARDAVNPDFLTDEDRERPATELLCRVVAELPRRLAQEPDDLVVCHGDLCLPNIVI
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEE
APGDFSVAGFIDLGRLGLADRHADLALLFANARETWPGDGWVTAAAHERFLNRYGASADP
ECCCCCEEHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCEEEHHHHHHHHHHHCCCCCH
ERLAFYLRLDPLTWG
HHEEEEEEECCCCCC
>Mature Secondary Structure
MIDLSAWEPIVTGESGACVYRSPDGSRYAKKGGADLAGERDRIEWLSAQRIPGPSVLEWA
CCCCCCCCCEEECCCCEEEEECCCCCHHHHCCCCCCCCCCHHHHHHHHCCCCCCCEEEEE
TTSDGPLLITSAVPGVPADQLDSEDLGRAWSGIAAAVRDLHALPTAGCPFSRGLAAMATL
ECCCCCEEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
ARDVVARDAVNPDFLTDEDRERPATELLCRVVAELPRRLAQEPDDLVVCHGDLCLPNIVI
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCEEE
APGDFSVAGFIDLGRLGLADRHADLALLFANARETWPGDGWVTAAAHERFLNRYGASADP
ECCCCCEEHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCEEEHHHHHHHHHHHCCCCCH
ERLAFYLRLDPLTWG
HHEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2167474; 2844130; 2821169 [H]