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The map label for this gene is tyrS [H]

Identifier: 169629440

GI number: 169629440

Start: 2409009

End: 2410322

Strand: Direct

Name: tyrS [H]

Synonym: MAB_2354

Alternate gene names: 169629440

Gene position: 2409009-2410322 (Clockwise)

Preceding gene: 169629439

Following gene: 169629442

Centisome position: 47.54

GC content: 65.3

Gene sequence:

>1314_bases
GTGGCTTCCCCGACTCATGATCTTCGTGTAAGCGGCTCATCCATCCTTGACGAGCTGACGTGGCGCGGCCTCATCGCGCA
AACGACCGACCGCGACGCGCTCGCGGCCGATATCGCGAAGGGCCCGATCAGTGTGTATGGAGGGTTTGACCCCACTGCCG
CGAGTCTGCACGCCGGGCACCTGGTGCCGTTGCTCACCCTGAGTCGATTCCAGCGGGCCGGCCACCGGCCCATCGTGCTG
GCCGGGGGAGCCACCGGCCTCATCGGGGACCCCCGCGACACCTCCGAACGAAACCTGCACGGAACAGACGTCGTCGCAGA
ATGGGCCGACCGGATACGTGGGCAGCTGGAGCGATTCGTCGAATTCAACGACACCCCAACCGGAGCGATCGTCGCGAACA
ATCTTGAATGGACCGGTCAGCTGACCGCCCTAGAGTTTCTGCGCGATCTGGGTAAACACTTCTCGGTCAACGTGATGCTC
GACCGTGACACCATCCGCCGGCGCCTGGAAGGCGACGGCATCTCCTACACCGAATTCTCGTACATGCTGCTGCAGGCCAA
CGACTACGTGCAGTTGCACCGCAAGTACGGCTGCTCATTGCAGATCGGCGGATCGGATCAGTGGGGCAACATCGTTGCCG
GAGTTCGGCTTGCCCGTCAGCAGGACGGTGCGTCGGTGCATGCCTTGACAGTTCCGCTGGTGACGGCGGCCGACGGCACC
AAGTTCGGCAAGTCCACCGGCGGCGGCAATCTGTGGCTCGATCCCGAGATGACGAGCCCGTACGCCTGGTACCAGTACTT
CATCAACACCGCCGACGCTGACGTCGTCCGGTACCTGCGCTGGTTCACGTTCCTGTCTGCCGAAGAGATCGGCGAACTGG
AGACGGCGACCACGGAACGCGCGCACGAGCGTGCCGCCCAGCGCCGGTTGGCAGCCGAGGTCACCACGCTCGTGCATGGC
GAAAGCGCCACGCTGGCGGTCGAACACGCCAGCGGTGCGCTGTTCGGCCGCGGCGATTTGGACCGGCTTGACGAGGGCAC
CCTGACCGCGGCTCTTACCGAAGCGGGTAATGGCGAACCGGCGCGGATCGCCGACGGCGAACCCGACACCATCGTGGATC
TCCTGGTGAGCAGCGGACTTTCGGAAAGCAAGGGTGCTGCGCGCCGCACCGTCAAGGAGGGCGGCGTATACGTCAACAAC
ACCCGGGTGGACGCTGAGGATTGGACCCCGGGCGATGGCGACTACCTCACCGGAGGTTGGCTGGTGCTGCGCCGCGGCAA
GCGCAATATCGCCGGGGTGCAGCGGCTCCGCTAG

Upstream 100 bases:

>100_bases
GGTCATCACGACGATCGCCGACGACCTCGTCGGGCCGCCGGGACCAGACTGGGATGAGCGGTATCCGCCGCGTTAGACCC
CATAGGGGATGATTGTCCGC

Downstream 100 bases:

>100_bases
CCGATCGCTCCGTTATACCCATTGGGGTTCAACGGATTCCCTTACCTTGCCGTCCTCGCCGAAGATGAGGAAACGGGTGA
AAGTTCGGGCGAACCACCGA

Product: tyrosyl-tRNA synthetase (TyrS)

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MASPTHDLRVSGSSILDELTWRGLIAQTTDRDALAADIAKGPISVYGGFDPTAASLHAGHLVPLLTLSRFQRAGHRPIVL
AGGATGLIGDPRDTSERNLHGTDVVAEWADRIRGQLERFVEFNDTPTGAIVANNLEWTGQLTALEFLRDLGKHFSVNVML
DRDTIRRRLEGDGISYTEFSYMLLQANDYVQLHRKYGCSLQIGGSDQWGNIVAGVRLARQQDGASVHALTVPLVTAADGT
KFGKSTGGGNLWLDPEMTSPYAWYQYFINTADADVVRYLRWFTFLSAEEIGELETATTERAHERAAQRRLAAEVTTLVHG
ESATLAVEHASGALFGRGDLDRLDEGTLTAALTEAGNGEPARIADGEPDTIVDLLVSSGLSESKGAARRTVKEGGVYVNN
TRVDAEDWTPGDGDYLTGGWLVLRRGKRNIAGVQRLR

Sequences:

>Translated_437_residues
MASPTHDLRVSGSSILDELTWRGLIAQTTDRDALAADIAKGPISVYGGFDPTAASLHAGHLVPLLTLSRFQRAGHRPIVL
AGGATGLIGDPRDTSERNLHGTDVVAEWADRIRGQLERFVEFNDTPTGAIVANNLEWTGQLTALEFLRDLGKHFSVNVML
DRDTIRRRLEGDGISYTEFSYMLLQANDYVQLHRKYGCSLQIGGSDQWGNIVAGVRLARQQDGASVHALTVPLVTAADGT
KFGKSTGGGNLWLDPEMTSPYAWYQYFINTADADVVRYLRWFTFLSAEEIGELETATTERAHERAAQRRLAAEVTTLVHG
ESATLAVEHASGALFGRGDLDRLDEGTLTAALTEAGNGEPARIADGEPDTIVDLLVSSGLSESKGAARRTVKEGGVYVNN
TRVDAEDWTPGDGDYLTGGWLVLRRGKRNIAGVQRLR
>Mature_436_residues
ASPTHDLRVSGSSILDELTWRGLIAQTTDRDALAADIAKGPISVYGGFDPTAASLHAGHLVPLLTLSRFQRAGHRPIVLA
GGATGLIGDPRDTSERNLHGTDVVAEWADRIRGQLERFVEFNDTPTGAIVANNLEWTGQLTALEFLRDLGKHFSVNVMLD
RDTIRRRLEGDGISYTEFSYMLLQANDYVQLHRKYGCSLQIGGSDQWGNIVAGVRLARQQDGASVHALTVPLVTAADGTK
FGKSTGGGNLWLDPEMTSPYAWYQYFINTADADVVRYLRWFTFLSAEEIGELETATTERAHERAAQRRLAAEVTTLVHGE
SATLAVEHASGALFGRGDLDRLDEGTLTAALTEAGNGEPARIADGEPDTIVDLLVSSGLSESKGAARRTVKEGGVYVNNT
RVDAEDWTPGDGDYLTGGWLVLRRGKRNIAGVQRLR

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=410, Percent_Identity=36.5853658536585, Blast_Score=213, Evalue=3e-55,
Organism=Escherichia coli, GI1787925, Length=425, Percent_Identity=48.2352941176471, Blast_Score=396, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI25145802, Length=312, Percent_Identity=38.4615384615385, Blast_Score=204, Evalue=7e-53,
Organism=Caenorhabditis elegans, GI25145805, Length=224, Percent_Identity=37.0535714285714, Blast_Score=146, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6325160, Length=460, Percent_Identity=36.0869565217391, Blast_Score=254, Evalue=2e-68,
Organism=Drosophila melanogaster, GI20130383, Length=449, Percent_Identity=38.5300668151448, Blast_Score=271, Evalue=6e-73,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 47443; Mature: 47312

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASPTHDLRVSGSSILDELTWRGLIAQTTDRDALAADIAKGPISVYGGFDPTAASLHAGH
CCCCCCCEEECCHHHHHHHHHCCCEEECCCCHHHHHHHCCCCEEEECCCCCCHHHHCCCC
LVPLLTLSRFQRAGHRPIVLAGGATGLIGDPRDTSERNLHGTDVVAEWADRIRGQLERFV
HHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
EFNDTPTGAIVANNLEWTGQLTALEFLRDLGKHFSVNVMLDRDTIRRRLEGDGISYTEFS
HCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEECHHHHHHHHCCCCCCEEEEE
YMLLQANDYVQLHRKYGCSLQIGGSDQWGNIVAGVRLARQQDGASVHALTVPLVTAADGT
EEEEECCCHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEEEECCCC
KFGKSTGGGNLWLDPEMTSPYAWYQYFINTADADVVRYLRWFTFLSAEEIGELETATTER
CCCCCCCCCCEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHCCHHHHHHHH
AHERAAQRRLAAEVTTLVHGESATLAVEHASGALFGRGDLDRLDEGTLTAALTEAGNGEP
HHHHHHHHHHHHHHHHHEECCCCEEEEEECCCCEECCCCCCCCCCCCEEEEEECCCCCCC
ARIADGEPDTIVDLLVSSGLSESKGAARRTVKEGGVYVNNTRVDAEDWTPGDGDYLTGGW
CEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHCCEEEECCEECCCCCCCCCCCEECCCE
LVLRRGKRNIAGVQRLR
EEEECCCCCHHHHHHCC
>Mature Secondary Structure 
ASPTHDLRVSGSSILDELTWRGLIAQTTDRDALAADIAKGPISVYGGFDPTAASLHAGH
CCCCCCEEECCHHHHHHHHHCCCEEECCCCHHHHHHHCCCCEEEECCCCCCHHHHCCCC
LVPLLTLSRFQRAGHRPIVLAGGATGLIGDPRDTSERNLHGTDVVAEWADRIRGQLERFV
HHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
EFNDTPTGAIVANNLEWTGQLTALEFLRDLGKHFSVNVMLDRDTIRRRLEGDGISYTEFS
HCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEECHHHHHHHHCCCCCCEEEEE
YMLLQANDYVQLHRKYGCSLQIGGSDQWGNIVAGVRLARQQDGASVHALTVPLVTAADGT
EEEEECCCHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEEEECCCC
KFGKSTGGGNLWLDPEMTSPYAWYQYFINTADADVVRYLRWFTFLSAEEIGELETATTER
CCCCCCCCCCEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHCCHHHHHHHH
AHERAAQRRLAAEVTTLVHGESATLAVEHASGALFGRGDLDRLDEGTLTAALTEAGNGEP
HHHHHHHHHHHHHHHHHEECCCCEEEEEECCCCEECCCCCCCCCCCCEEEEEECCCCCCC
ARIADGEPDTIVDLLVSSGLSESKGAARRTVKEGGVYVNNTRVDAEDWTPGDGDYLTGGW
CEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHCCEEEECCEECCCCCCCCCCCEECCCE
LVLRRGKRNIAGVQRLR
EEEECCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]