BacMap

Definition	Mycobacterium abscessus ATCC 19977 chromosome chromosome 1, complete sequence.
Accession	NC_010397
Length	5,067,172

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The map label for this gene is ephD [H]

Identifier: 169629157

GI number: 169629157

Start: 2070325

End: 2071179

Strand: Direct

Name: ephD [H]

Synonym: MAB_2071

Alternate gene names: 169629157

Gene position: 2070325-2071179 (Clockwise)

Preceding gene: 169629155

Following gene: 169629158

Centisome position: 40.86

GC content: 60.7

Gene sequence:

>855_bases
ATGAGATTGCTGCCATTCGGCGGCGCACCCGGCAGAACGCACCGAGCCGATGCAGTCGTCACGGGCGCAGGAAGCGGTAT
CGGCCGCGCATTCGCCGTGGAGCTTGCACGCCGAGGCGGACGCGTGGTGTGCGCCGACAGAGATCCCGTCACCGCAAAAG
AGTCGGCAGAGTTGGTGAGGCAGGCTGGCGGCGAAGGCTTCGACATCGCATGCGACGTCACCGATCTTGAGCAGATCCGC
AACCTCGCTGATGTGAGCGAAGACTGGTTCGGGAAGGCCGCGAGCCTCGTCATCAATAACGCCGGAATCGGTGCAGGTGG
TAACCGAATCGGCGCGACGTCAATCGAGGACTGGAAAGCGGCGATTTCGGTCAACCTCTGGGGCGTTATCTACGGCTGCG
AGACTTTTGTGCCGCGGCTCCGCAGCAGTGGGCATGGCGGAGTCATCAATGTGGCGTCCGCAGCGAGCTTTGGCTCGGCC
CCTCGAATGGCGGCTTACAACGTGAGCAAGGCCGGAGTCCTCGCTCTGTCGGAGACATTGGCGGCCGAACTGAGCGGTAC
TGACGTCAAGGTCACAGTGCTTTGCCCCACCTTCGTGAAGACGAACATCGTCAAAAATCCTCAAATCGACGAGGCGGCTG
CGAGGCTCGCGGCCAACCTGATGAAATGGACTGGCATTTCGCCACAGCACGTTGCTCGAACGACGTTGAACGCGCATGAT
CGCGGACAAATTTATGTAGTGCCCCAACTCGATGCCAAAGTCTTGTGGCAACTCAAGAGAGCCCTACCCGGTCCTTTTAC
ACGCACGCTGGGCCTCGTGGAGCGCATGGCCTCATGGAACGATTCAGCCGAGTAG

Upstream 100 bases:

>100_bases
ATGACAAAACTGGTGGCATGCGACACCAATGGTGGCTGATGCCCCCAGATGAGAGTTCCCCCACATCACACGTCAATGTC
GCGACCCGGAAGGCAGACAG

Downstream 100 bases:

>100_bases
AGGAGAAGGAAAAATGGCTTTCGCATACAGCGACATGCTCGAGACAATCAAAAACAAGCAATGGGCTCTAGCCGATATCG
ATTGGGACGCCCCTGGCGCG

Product: short-chain dehydrogenase/reductase

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MRLLPFGGAPGRTHRADAVVTGAGSGIGRAFAVELARRGGRVVCADRDPVTAKESAELVRQAGGEGFDIACDVTDLEQIR
NLADVSEDWFGKAASLVINNAGIGAGGNRIGATSIEDWKAAISVNLWGVIYGCETFVPRLRSSGHGGVINVASAASFGSA
PRMAAYNVSKAGVLALSETLAAELSGTDVKVTVLCPTFVKTNIVKNPQIDEAAARLAANLMKWTGISPQHVARTTLNAHD
RGQIYVVPQLDAKVLWQLKRALPGPFTRTLGLVERMASWNDSAE

Sequences:

>Translated_284_residues
MRLLPFGGAPGRTHRADAVVTGAGSGIGRAFAVELARRGGRVVCADRDPVTAKESAELVRQAGGEGFDIACDVTDLEQIR
NLADVSEDWFGKAASLVINNAGIGAGGNRIGATSIEDWKAAISVNLWGVIYGCETFVPRLRSSGHGGVINVASAASFGSA
PRMAAYNVSKAGVLALSETLAAELSGTDVKVTVLCPTFVKTNIVKNPQIDEAAARLAANLMKWTGISPQHVARTTLNAHD
RGQIYVVPQLDAKVLWQLKRALPGPFTRTLGLVERMASWNDSAE
>Mature_284_residues
MRLLPFGGAPGRTHRADAVVTGAGSGIGRAFAVELARRGGRVVCADRDPVTAKESAELVRQAGGEGFDIACDVTDLEQIR
NLADVSEDWFGKAASLVINNAGIGAGGNRIGATSIEDWKAAISVNLWGVIYGCETFVPRLRSSGHGGVINVASAASFGSA
PRMAAYNVSKAGVLALSETLAAELSGTDVKVTVLCPTFVKTNIVKNPQIDEAAARLAANLMKWTGISPQHVARTTLNAHD
RGQIYVVPQLDAKVLWQLKRALPGPFTRTLGLVERMASWNDSAE

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI142976729, Length=192, Percent_Identity=32.2916666666667, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI210032110, Length=267, Percent_Identity=26.2172284644195, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI40807363, Length=250, Percent_Identity=26, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI31542939, Length=226, Percent_Identity=29.646017699115, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI310128830, Length=282, Percent_Identity=26.241134751773, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI310115468, Length=282, Percent_Identity=26.241134751773, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI25282469, Length=220, Percent_Identity=28.1818181818182, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI40254992, Length=187, Percent_Identity=29.4117647058824, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI15277342, Length=206, Percent_Identity=33.0097087378641, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI32483357, Length=197, Percent_Identity=28.4263959390863, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI33667109, Length=225, Percent_Identity=28.8888888888889, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI5031737, Length=191, Percent_Identity=31.413612565445, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI126723750, Length=196, Percent_Identity=28.0612244897959, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI19923817, Length=193, Percent_Identity=27.979274611399, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI4557649, Length=191, Percent_Identity=27.2251308900524, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI10190746, Length=258, Percent_Identity=29.0697674418605, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI126723191, Length=180, Percent_Identity=27.7777777777778, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI4504505, Length=199, Percent_Identity=27.6381909547739, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI44680136, Length=120, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI44680133, Length=120, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI17738292, Length=120, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI150247226, Length=224, Percent_Identity=31.25, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI224922801, Length=173, Percent_Identity=30.635838150289, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI20149619, Length=247, Percent_Identity=25.5060728744939, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI31543615, Length=235, Percent_Identity=25.531914893617, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1787905, Length=226, Percent_Identity=26.9911504424779, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1789208, Length=195, Percent_Identity=28.2051282051282, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787335, Length=201, Percent_Identity=29.8507462686567, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI2367175, Length=193, Percent_Identity=31.6062176165803, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787820, Length=230, Percent_Identity=27.3913043478261, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786812, Length=192, Percent_Identity=28.6458333333333, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786701, Length=196, Percent_Identity=26.530612244898, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87082100, Length=190, Percent_Identity=25.2631578947368, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI193203117, Length=187, Percent_Identity=29.9465240641711, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI193203115, Length=188, Percent_Identity=29.7872340425532, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17564282, Length=204, Percent_Identity=28.921568627451, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17508895, Length=204, Percent_Identity=26.4705882352941, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17567345, Length=189, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI25147288, Length=190, Percent_Identity=32.6315789473684, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71994604, Length=197, Percent_Identity=30.4568527918782, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17561402, Length=187, Percent_Identity=28.8770053475936, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17559104, Length=190, Percent_Identity=30.5263157894737, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17562910, Length=202, Percent_Identity=31.1881188118812, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17538486, Length=191, Percent_Identity=30.3664921465969, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17555706, Length=203, Percent_Identity=28.0788177339901, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17555612, Length=293, Percent_Identity=24.2320819112628, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17560676, Length=195, Percent_Identity=25.1282051282051, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI71994600, Length=199, Percent_Identity=29.1457286432161, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17568967, Length=189, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17565030, Length=191, Percent_Identity=29.8429319371728, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17507613, Length=199, Percent_Identity=30.6532663316583, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI32563809, Length=199, Percent_Identity=30.6532663316583, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6320089, Length=195, Percent_Identity=28.2051282051282, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323882, Length=202, Percent_Identity=26.2376237623762, Blast_Score=73, Evalue=4e-14,
Organism=Drosophila melanogaster, GI28571526, Length=194, Percent_Identity=33.5051546391753, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI281364892, Length=227, Percent_Identity=26.431718061674, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24649181, Length=191, Percent_Identity=29.3193717277487, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24640492, Length=194, Percent_Identity=25.2577319587629, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI21357041, Length=196, Percent_Identity=31.1224489795918, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24640442, Length=199, Percent_Identity=31.1557788944724, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI23397609, Length=197, Percent_Identity=35.0253807106599, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17737361, Length=257, Percent_Identity=28.4046692607004, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI221510726, Length=261, Percent_Identity=27.2030651340996, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356301, Length=259, Percent_Identity=29.7297297297297, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24639444, Length=190, Percent_Identity=28.4210526315789, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI161076933, Length=189, Percent_Identity=26.4550264550265, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24584748, Length=189, Percent_Identity=26.4550264550265, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI281364494, Length=199, Percent_Identity=32.1608040201005, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI116007236, Length=247, Percent_Identity=28.7449392712551, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI21358495, Length=273, Percent_Identity=22.7106227106227, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI18859837, Length=192, Percent_Identity=29.6875, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21357673, Length=202, Percent_Identity=28.7128712871287, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24644339, Length=186, Percent_Identity=29.5698924731183, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21355319, Length=200, Percent_Identity=27.5, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI116007238, Length=207, Percent_Identity=28.9855072463768, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI161076371, Length=239, Percent_Identity=27.6150627615063, Blast_Score=64, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 29905; Mature: 29905

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLPFGGAPGRTHRADAVVTGAGSGIGRAFAVELARRGGRVVCADRDPVTAKESAELVR
CEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHH
QAGGEGFDIACDVTDLEQIRNLADVSEDWFGKAASLVINNAGIGAGGNRIGATSIEDWKA
HCCCCCCEEEECHHHHHHHHHHHHCCHHHHCHHEEEEEECCCCCCCCCCCCCCCHHHHHE
AISVNLWGVIYGCETFVPRLRSSGHGGVINVASAASFGSAPRMAAYNVSKAGVLALSETL
EEEEEEEEEEHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHH
AAELSGTDVKVTVLCPTFVKTNIVKNPQIDEAAARLAANLMKWTGISPQHVARTTLNAHD
HHHCCCCCEEEEEEECHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
RGQIYVVPQLDAKVLWQLKRALPGPFTRTLGLVERMASWNDSAE
CCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRLLPFGGAPGRTHRADAVVTGAGSGIGRAFAVELARRGGRVVCADRDPVTAKESAELVR
CEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHH
QAGGEGFDIACDVTDLEQIRNLADVSEDWFGKAASLVINNAGIGAGGNRIGATSIEDWKA
HCCCCCCEEEECHHHHHHHHHHHHCCHHHHCHHEEEEEECCCCCCCCCCCCCCCHHHHHE
AISVNLWGVIYGCETFVPRLRSSGHGGVINVASAASFGSAPRMAAYNVSKAGVLALSETL
EEEEEEEEEEHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHH
AAELSGTDVKVTVLCPTFVKTNIVKNPQIDEAAARLAANLMKWTGISPQHVARTTLNAHD
HHHCCCCCEEEEEEECHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
RGQIYVVPQLDAKVLWQLKRALPGPFTRTLGLVERMASWNDSAE
CCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.085 {glycochenodeoxycholic} 0.24 {taurochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]