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The map label for this gene is caiD [H]

Identifier: 169629145

GI number: 169629145

Start: 2056779

End: 2057546

Strand: Direct

Name: caiD [H]

Synonym: MAB_2058

Alternate gene names: 169629145

Gene position: 2056779-2057546 (Clockwise)

Preceding gene: 169629144

Following gene: 169629146

Centisome position: 40.59

GC content: 68.75

Gene sequence:

>768_bases
GTGGCCGAGCAACCGATTCGCTACGAGGTCGTCGACAGCGTGGCCTGGCTGACGATCAACCGGCCCGAGGCGCGCAACGC
GCTGAACAACGCTGTGCGCACGGGGCTTATCGACGCGGTACTCCGCTTCAATGACGACGACGCGGCGAAGGTGCTCGTCC
TCACCGGCGTGGGCGATAAGGCGTTCTGCGCCGGCGGGGACTTGAAGGAGATGGCGCAGAACGCGCTCAAGGTGCCCCCG
AAGGACTTCGCTCCGCAGTTCGGCCGCAACATCGATGTCGCGAAGCCGACGATCGCGGCCGTCAACGGTGTCGCGTTCGC
CGGGGGCTTCCTGCTCGCGCAGCAGTGCGACCTGGTCGTGGCCGCCGAACACGCGACCTTCGCCGTCAGCGAGGTCAAGG
TCGGCCGCGGTTCGCCGTGGGCGACACCCCTGTCGTGGCTGGTGCCCCCGCGCGTTGCCATGCAAATCCTGCTGACCGGC
GACCCGATCACCGCCGCGCGCGCCCACCAGGTCGGCTTGGTCAATGAGGTGGTGCCGGCCGATCAGTTACGCGAGCGTAC
CCAGCAGTTGGCGCTCAGCATCGCCGCGAACGCACCGCTGTCCGTGCTCGCGTCCAAGCGCACCGTGTACCTCTCGGCGC
AGCACCACCTCGCCGCCGCGTACGACCTGGCCGACGAGATATGGGAGCCGGTCTATCTGAGCGACGACGCGCAGGAAGGC
CCGGCGGCGTTCCGCGAGAAGCGCGCGCCGCAGTGGAAGGGACACTGA

Upstream 100 bases:

>100_bases
GTGCCGAGTGAAGCCACCGTTGAGCTGGCCGACCCGTTTGTCGAGGTGCCGGGGTGAACTGCCACTCACATGCGAGACGA
CGGTGAGGGACGGAGGCTCG

Downstream 100 bases:

>100_bases
CATGGCGGTGAGCATGCAGTCGGTTATCACCGACATCGAAGCGGAGACGGCAGCCCTACGCGGATTGGTCGCGCCGCTGA
CCGAAGGTCCGCGCGGCTGG

Product: enoyl-CoA dehydratase/isomerase

Products: trans(or 3)-enoyl-CoA; H2O [C]

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MAEQPIRYEVVDSVAWLTINRPEARNALNNAVRTGLIDAVLRFNDDDAAKVLVLTGVGDKAFCAGGDLKEMAQNALKVPP
KDFAPQFGRNIDVAKPTIAAVNGVAFAGGFLLAQQCDLVVAAEHATFAVSEVKVGRGSPWATPLSWLVPPRVAMQILLTG
DPITAARAHQVGLVNEVVPADQLRERTQQLALSIAANAPLSVLASKRTVYLSAQHHLAAAYDLADEIWEPVYLSDDAQEG
PAAFREKRAPQWKGH

Sequences:

>Translated_255_residues
MAEQPIRYEVVDSVAWLTINRPEARNALNNAVRTGLIDAVLRFNDDDAAKVLVLTGVGDKAFCAGGDLKEMAQNALKVPP
KDFAPQFGRNIDVAKPTIAAVNGVAFAGGFLLAQQCDLVVAAEHATFAVSEVKVGRGSPWATPLSWLVPPRVAMQILLTG
DPITAARAHQVGLVNEVVPADQLRERTQQLALSIAANAPLSVLASKRTVYLSAQHHLAAAYDLADEIWEPVYLSDDAQEG
PAAFREKRAPQWKGH
>Mature_254_residues
AEQPIRYEVVDSVAWLTINRPEARNALNNAVRTGLIDAVLRFNDDDAAKVLVLTGVGDKAFCAGGDLKEMAQNALKVPPK
DFAPQFGRNIDVAKPTIAAVNGVAFAGGFLLAQQCDLVVAAEHATFAVSEVKVGRGSPWATPLSWLVPPRVAMQILLTGD
PITAARAHQVGLVNEVVPADQLRERTQQLALSIAANAPLSVLASKRTVYLSAQHHLAAAYDLADEIWEPVYLSDDAQEGP
AAFREKRAPQWKGH

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=245, Percent_Identity=37.5510204081633, Blast_Score=151, Evalue=5e-37,
Organism=Homo sapiens, GI31542718, Length=258, Percent_Identity=31.3953488372093, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI70995211, Length=231, Percent_Identity=29.4372294372294, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI45643119, Length=251, Percent_Identity=25.4980079681275, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI260275230, Length=251, Percent_Identity=25.4980079681275, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI260274832, Length=251, Percent_Identity=25.4980079681275, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI4502327, Length=256, Percent_Identity=27.734375, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI37594469, Length=193, Percent_Identity=25.9067357512953, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI37594471, Length=193, Percent_Identity=25.9067357512953, Blast_Score=66, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=245, Percent_Identity=38.3673469387755, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI221142681, Length=253, Percent_Identity=35.5731225296443, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1788597, Length=260, Percent_Identity=33.0769230769231, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI87082183, Length=263, Percent_Identity=28.8973384030418, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787660, Length=266, Percent_Identity=32.7067669172932, Blast_Score=96, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25145438, Length=249, Percent_Identity=37.7510040160643, Blast_Score=153, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI17554946, Length=261, Percent_Identity=34.8659003831418, Blast_Score=140, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI17560910, Length=225, Percent_Identity=33.3333333333333, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17540714, Length=222, Percent_Identity=26.5765765765766, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17540306, Length=202, Percent_Identity=33.1683168316832, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17535521, Length=257, Percent_Identity=27.6264591439689, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17534483, Length=228, Percent_Identity=27.1929824561404, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17549921, Length=248, Percent_Identity=27.0161290322581, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=199, Percent_Identity=26.1306532663317, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI20129971, Length=253, Percent_Identity=34.3873517786561, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24653477, Length=253, Percent_Identity=34.3873517786561, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24653139, Length=254, Percent_Identity=29.1338582677165, Blast_Score=107, Evalue=5e-24,
Organism=Drosophila melanogaster, GI19922422, Length=251, Percent_Identity=30.2788844621514, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI21357171, Length=219, Percent_Identity=29.6803652968037, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24650670, Length=261, Percent_Identity=27.9693486590038, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24654903, Length=175, Percent_Identity=26.8571428571429, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17 [C]

Molecular weight: Translated: 27417; Mature: 27285

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQPIRYEVVDSVAWLTINRPEARNALNNAVRTGLIDAVLRFNDDDAAKVLVLTGVGDK
CCCCCCHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
AFCAGGDLKEMAQNALKVPPKDFAPQFGRNIDVAKPTIAAVNGVAFAGGFLLAQQCDLVV
CEECCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCEEE
AAEHATFAVSEVKVGRGSPWATPLSWLVPPRVAMQILLTGDPITAARAHQVGLVNEVVPA
EECCCEEEEEEEECCCCCCCCCCHHHHCCHHHEEEEEECCCCCCHHHHHHHCHHHHCCCH
DQLRERTQQLALSIAANAPLSVLASKRTVYLSAQHHLAAAYDLADEIWEPVYLSDDAQEG
HHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCEEECCCCCCC
PAAFREKRAPQWKGH
HHHHHHHCCCCCCCC
>Mature Secondary Structure 
AEQPIRYEVVDSVAWLTINRPEARNALNNAVRTGLIDAVLRFNDDDAAKVLVLTGVGDK
CCCCCHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
AFCAGGDLKEMAQNALKVPPKDFAPQFGRNIDVAKPTIAAVNGVAFAGGFLLAQQCDLVV
CEECCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCEEE
AAEHATFAVSEVKVGRGSPWATPLSWLVPPRVAMQILLTGDPITAARAHQVGLVNEVVPA
EECCCEEEEEEEECCCCCCCCCCHHHHCCHHHEEEEEECCCCCCHHHHHHHCHHHHCCCH
DQLRERTQQLALSIAANAPLSVLASKRTVYLSAQHHLAAAYDLADEIWEPVYLSDDAQEG
HHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCEEECCCCCCC
PAAFREKRAPQWKGH
HHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-hydroxyacyl-CoA [C]

Specific reaction: (3S)-hydroxyacyl-CoA = trans(or 3)-enoyl-CoA + H2O [C]

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA