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The map label for this gene is ilvE [H]

Identifier: 169629037

GI number: 169629037

Start: 1948485

End: 1949588

Strand: Direct

Name: ilvE [H]

Synonym: MAB_1950

Alternate gene names: 169629037

Gene position: 1948485-1949588 (Clockwise)

Preceding gene: 169629036

Following gene: 169629043

Centisome position: 38.45

GC content: 67.21

Gene sequence:

>1104_bases
ATGAATCCGCTTCCCGCGTTCACCCGGCAACTCAACCCGTCACCGGCCAGCCCGGCGCGGCGGGCTGAGGTACTCGCGGC
GCCGGGATTCGGGAAGTACTTCACCGACCACATGGTGTCCATCGACTGGAACGCCGAAAACGGCTGGCACAACGCGCAAG
TGGTGCCTTATGGTCCGATCACGCTTGACCCGTCGGCCATCGTGTTGCACTACGCACAAGAGGTCTTCGAGGGACTCAAG
GCGTACCGGCAACCCGACGGAACCATCGCGGCGTTTCGCCCCGAGGCTAACGGTGAACGGCTGATCCAGTCCTGCCGCCG
CATCGCCATCCCCGAGCTGCCGCTGGAGCTGTTCATCGAGTCGCTGCGCCAGCTCATCGCGGTGGATGCCGAGTGGGTGC
CGCCGGGCGGCGGCGAGGAATCGCTCTACCTGCGGCCGTTCATCATCGCCACCGAGGCAGGCCTGGGGGTGCGGCCCGCC
GGCGAGTACCGTTACCTGCTCATCGCCTCACCGGCCGGTGCTTACTTCTCTCAGGGCATCAAGCCGGTCAGCGTGTGGCT
CTCGCACGAGTACGTGCGAGCCGCGCCGGGCGGTATCGGCGCGGCCAAGACCGGCGGCAACTACGCCGCCTCGCTGGTCG
CTCAGGCGCAGGCCGCCGAGGAAGGCTGCGACCAGGTGGTGTGGCTCGACGCCATCGAGCGCCGGTACATCGAAGAAATG
GGTGGCATGAACCTGTTCTTCGTCTTCGGGCGCGACGGTGAGGCGCGCCTGGTCACCCCCGAGCTGTCCGGGGCGCTGCT
GCCGGGCGTGACGCGGAAGTCCTTGTTGCAGCTGGCCTCCGACGCCGGTTTTGCCATCGAGGAACGCAAGATCGATGTCG
ACGAGCTGGAGAAGAAGACGGCCTCGGGGGAGATCACCGAGGTGTTCGCCTGCGGCACGGCGGCGGTCATCACACCGGTC
GGCCGCGTCAAGCACTCGGGCGGCGAATTCACCATCGGTGACGGGGAACCCGGCGAGGTGACCATGGCCCTGCGGGACAC
ACTGACGGGAATCCAGCGCGGTACCTTCGCCGATACGCACGGCTGGATCACCCAGCTCGGCTAG

Upstream 100 bases:

>100_bases
CCGTGAGATCGAATGCGAGGTCGTCAAGCCGCCATTCGTGGACGTCAACGTCGGATAATGGGTTCGGATTCGGCGACGGC
AGACGGTTAGACTGCCGAGC

Downstream 100 bases:

>100_bases
CGCACTGCCGCGTGAACCCGGCGGCGATACGTCACCTTGTCGAACTCCCGGCCATATTCGTCCACGGCGGTGACGTCCAT
CTCGCTGACCAGTGCTCCGG

Product: branched-chain amino acid aminotransferase (IlvE)

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MNPLPAFTRQLNPSPASPARRAEVLAAPGFGKYFTDHMVSIDWNAENGWHNAQVVPYGPITLDPSAIVLHYAQEVFEGLK
AYRQPDGTIAAFRPEANGERLIQSCRRIAIPELPLELFIESLRQLIAVDAEWVPPGGGEESLYLRPFIIATEAGLGVRPA
GEYRYLLIASPAGAYFSQGIKPVSVWLSHEYVRAAPGGIGAAKTGGNYAASLVAQAQAAEEGCDQVVWLDAIERRYIEEM
GGMNLFFVFGRDGEARLVTPELSGALLPGVTRKSLLQLASDAGFAIEERKIDVDELEKKTASGEITEVFACGTAAVITPV
GRVKHSGGEFTIGDGEPGEVTMALRDTLTGIQRGTFADTHGWITQLG

Sequences:

>Translated_367_residues
MNPLPAFTRQLNPSPASPARRAEVLAAPGFGKYFTDHMVSIDWNAENGWHNAQVVPYGPITLDPSAIVLHYAQEVFEGLK
AYRQPDGTIAAFRPEANGERLIQSCRRIAIPELPLELFIESLRQLIAVDAEWVPPGGGEESLYLRPFIIATEAGLGVRPA
GEYRYLLIASPAGAYFSQGIKPVSVWLSHEYVRAAPGGIGAAKTGGNYAASLVAQAQAAEEGCDQVVWLDAIERRYIEEM
GGMNLFFVFGRDGEARLVTPELSGALLPGVTRKSLLQLASDAGFAIEERKIDVDELEKKTASGEITEVFACGTAAVITPV
GRVKHSGGEFTIGDGEPGEVTMALRDTLTGIQRGTFADTHGWITQLG
>Mature_367_residues
MNPLPAFTRQLNPSPASPARRAEVLAAPGFGKYFTDHMVSIDWNAENGWHNAQVVPYGPITLDPSAIVLHYAQEVFEGLK
AYRQPDGTIAAFRPEANGERLIQSCRRIAIPELPLELFIESLRQLIAVDAEWVPPGGGEESLYLRPFIIATEAGLGVRPA
GEYRYLLIASPAGAYFSQGIKPVSVWLSHEYVRAAPGGIGAAKTGGNYAASLVAQAQAAEEGCDQVVWLDAIERRYIEEM
GGMNLFFVFGRDGEARLVTPELSGALLPGVTRKSLLQLASDAGFAIEERKIDVDELEKKTASGEITEVFACGTAAVITPV
GRVKHSGGEFTIGDGEPGEVTMALRDTLTGIQRGTFADTHGWITQLG

Specific function: Catalyzes the reversible transfers of an amino group from glutamate to the alpha-ketoacid of the respective amino acid in the final step in the biosynthesis of branchedchain amino acids. The amino acids can be ranked in the following order with respect to

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI296010904, Length=329, Percent_Identity=41.6413373860182, Blast_Score=254, Evalue=8e-68,
Organism=Homo sapiens, GI38176287, Length=329, Percent_Identity=41.6413373860182, Blast_Score=254, Evalue=1e-67,
Organism=Homo sapiens, GI296010906, Length=329, Percent_Identity=41.6413373860182, Blast_Score=254, Evalue=1e-67,
Organism=Homo sapiens, GI50658084, Length=338, Percent_Identity=38.4615384615385, Blast_Score=237, Evalue=2e-62,
Organism=Homo sapiens, GI296010902, Length=329, Percent_Identity=37.3860182370821, Blast_Score=210, Evalue=2e-54,
Organism=Homo sapiens, GI296010900, Length=329, Percent_Identity=37.3860182370821, Blast_Score=209, Evalue=4e-54,
Organism=Homo sapiens, GI258614015, Length=294, Percent_Identity=37.7551020408163, Blast_Score=192, Evalue=5e-49,
Organism=Escherichia coli, GI48994963, Length=333, Percent_Identity=32.1321321321321, Blast_Score=127, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17568601, Length=317, Percent_Identity=41.0094637223975, Blast_Score=250, Evalue=9e-67,
Organism=Caenorhabditis elegans, GI17565728, Length=352, Percent_Identity=35.5113636363636, Blast_Score=211, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6322002, Length=318, Percent_Identity=43.3962264150943, Blast_Score=248, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6322608, Length=335, Percent_Identity=41.1940298507463, Blast_Score=238, Evalue=2e-63,
Organism=Drosophila melanogaster, GI24641779, Length=358, Percent_Identity=38.8268156424581, Blast_Score=235, Evalue=3e-62,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 39524; Mature: 39524

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPLPAFTRQLNPSPASPARRAEVLAAPGFGKYFTDHMVSIDWNAENGWHNAQVVPYGPI
CCCCCCHHHCCCCCCCCCHHHHEEEECCCCCHHHCCCEEEEEECCCCCCCCCEEEECCCE
TLDPSAIVLHYAQEVFEGLKAYRQPDGTIAAFRPEANGERLIQSCRRIAIPELPLELFIE
EECCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHH
SLRQLIAVDAEWVPPGGGEESLYLRPFIIATEAGLGVRPAGEYRYLLIASPAGAYFSQGI
HHHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCEEEEEEECCCCHHHHCCC
KPVSVWLSHEYVRAAPGGIGAAKTGGNYAASLVAQAQAAEEGCDQVVWLDAIERRYIEEM
CEEEEEECCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHH
GGMNLFFVFGRDGEARLVTPELSGALLPGVTRKSLLQLASDAGFAIEERKIDVDELEKKT
CCCEEEEEEECCCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCHHHHHHHC
ASGEITEVFACGTAAVITPVGRVKHSGGEFTIGDGEPGEVTMALRDTLTGIQRGTFADTH
CCCCEEEEEECCCHHHHCCHHHHCCCCCCEEECCCCCCEEEEHHHHHHHHHHCCCCCCCC
GWITQLG
CHHEECC
>Mature Secondary Structure
MNPLPAFTRQLNPSPASPARRAEVLAAPGFGKYFTDHMVSIDWNAENGWHNAQVVPYGPI
CCCCCCHHHCCCCCCCCCHHHHEEEECCCCCHHHCCCEEEEEECCCCCCCCCEEEECCCE
TLDPSAIVLHYAQEVFEGLKAYRQPDGTIAAFRPEANGERLIQSCRRIAIPELPLELFIE
EECCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHH
SLRQLIAVDAEWVPPGGGEESLYLRPFIIATEAGLGVRPAGEYRYLLIASPAGAYFSQGI
HHHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCEEEEEEECCCCHHHHCCC
KPVSVWLSHEYVRAAPGGIGAAKTGGNYAASLVAQAQAAEEGCDQVVWLDAIERRYIEEM
CEEEEEECCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHH
GGMNLFFVFGRDGEARLVTPELSGALLPGVTRKSLLQLASDAGFAIEERKIDVDELEKKT
CCCEEEEEEECCCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCHHHHHHHC
ASGEITEVFACGTAAVITPVGRVKHSGGEFTIGDGEPGEVTMALRDTLTGIQRGTFADTH
CCCCEEEEEECCCHHHHCCHHHHCCCCCCEEECCCCCCEEEEHHHHHHHHHHCCCCCCCC
GWITQLG
CHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA