Click here to switch to the map view.

The map label for this gene is adhP [C]

Identifier: 169628943

GI number: 169628943

Start: 1854093

End: 1855070

Strand: Reverse

Name: adhP [C]

Synonym: MAB_1855c

Alternate gene names: 169628943

Gene position: 1855070-1854093 (Counterclockwise)

Preceding gene: 169628945

Following gene: 169628942

Centisome position: 36.61

GC content: 68.3

Gene sequence:

>978_bases
ATGAAGGCAGCAGTCGTTCATGAGTGGGGGCAGGAGCCCGCCTACACCGACTTCCCGGACCCACAGCCCACCGGCGGCGC
CGAGGTCGCCACCGTCGAGGCGTCCGCGTTGACCAATCTCACCCGAGCCGTCATCTCCGGAAAACACTACAGCAGCAAGG
AAATAACCCTCCCGGCGATCGCCGGCGTGGACGGCGTCGCCCGGCTGGCCAATGGCAAGCGGGTGTACTCCGGCTCGCTC
GCGCCCTACGGGATGATGGCCGAACGCACCGTGGTGTACCCGGCCGGTGCCGTGGAGGTGCCCGAGCATGTCGACTCGGT
GACCGCTGCCGCGATACCCAATCCGGGGATATCGGCCTGGGTAGCCCTGTCCCATGGCGCCGCGGTGCAGCCCGGCCAGC
ACGTGCTGGTGCTGGGAGCCACCGGGGTCACCGGCTCACTTGCCGTGCAATTGGCGAAATCGGTGTTCGGCGCCGGGCAT
GTGACCGTCGCCGGCCGCAATGCCGAGCGGCTGGACTGGCTGAGGACGGTCGGCGCCGATGACGCGATCATGCTCGGCAG
CGACGACCTGACCGAGGCCATCACCGCGCGGCACGCCACGCGGCCGTTCGACGCGGTGCTCGACTACCTGTGGGGCTCCC
CCGCCGAACAGACGCTCAGCGCACTGGCGGCCAGTCACCCGTCGGCGCACTTCCACGCCACCCGGTTTGTCCAGATCGGT
GCAATGACCGGGGAAACAATCAGCGTGAACGCTTCCACCTTGCGCAGCACCGGCATCACCTTGTCCGGTGTCGGGATCGG
CAGCGTGCCGCCCGAGGCGCTGCAGCACGCCCGCACCGAGGCCCTGCCGAAACTCTTCGCCATGGTCAACAACGGAGACC
TGCAGCTACAGACACTGGCCCGGCCGCTCGCCGATATCGAAACCATCTGGGCGGCAAAGGAGCCGTCCGGGGTGCGGGTG
GTGGTCACTCCCCAGTAA

Upstream 100 bases:

>100_bases
GTTTTCATGGAAACCATATTGCCATCCGCCAGCCCGCGCCGTACAGTGTTGACATGGTTTCATTGGAAACCATTTCTATG
GAAACTTTGAGGAGGACACC

Downstream 100 bases:

>100_bases
CCTGCCGGTCGCCCGTTTCCGGTTTTCCCATCTGCCATTCGATGGCACGGTGGTACTCATGAACCACTCCGCCGAGCCCG
AGCACGACGAGCATTCCCGC

Product: putative zinc-binding alcohol dehydrogenase

Products: Glycerol; Nicotinamide adenine dinucleotide [C]

Alternate protein names: Quinone Oxidoreductase; Zinc-Containing Alcohol Dehydrogenase; NADPHQuinone Reductase; Oxidoreductase; Dehydrogenase; Alcohol Dehydrogenase GroES Domain Protein; NAD(P)H Quinone Oxidoreductase PIG3 Family; Zinc-Containing Alcohol Dehydrogenase Superfamily Protein; LOW QUALITY PROTEIN Quinone Oxidoreductase; NADPH2Quinone Reductase; Dehydrogenase/Oxidoreductase; Alcohol Dehydrogenase Zinc-Containing; Alcohol Dehydrogenase Protein; NADPHQuinone Reductase Related Zn-Dependent Oxidoreductase; Quinone Oxidoreductase Protein; Orf_Bo; NADPH Quinone Oxidoreductase

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MKAAVVHEWGQEPAYTDFPDPQPTGGAEVATVEASALTNLTRAVISGKHYSSKEITLPAIAGVDGVARLANGKRVYSGSL
APYGMMAERTVVYPAGAVEVPEHVDSVTAAAIPNPGISAWVALSHGAAVQPGQHVLVLGATGVTGSLAVQLAKSVFGAGH
VTVAGRNAERLDWLRTVGADDAIMLGSDDLTEAITARHATRPFDAVLDYLWGSPAEQTLSALAASHPSAHFHATRFVQIG
AMTGETISVNASTLRSTGITLSGVGIGSVPPEALQHARTEALPKLFAMVNNGDLQLQTLARPLADIETIWAAKEPSGVRV
VVTPQ

Sequences:

>Translated_325_residues
MKAAVVHEWGQEPAYTDFPDPQPTGGAEVATVEASALTNLTRAVISGKHYSSKEITLPAIAGVDGVARLANGKRVYSGSL
APYGMMAERTVVYPAGAVEVPEHVDSVTAAAIPNPGISAWVALSHGAAVQPGQHVLVLGATGVTGSLAVQLAKSVFGAGH
VTVAGRNAERLDWLRTVGADDAIMLGSDDLTEAITARHATRPFDAVLDYLWGSPAEQTLSALAASHPSAHFHATRFVQIG
AMTGETISVNASTLRSTGITLSGVGIGSVPPEALQHARTEALPKLFAMVNNGDLQLQTLARPLADIETIWAAKEPSGVRV
VVTPQ
>Mature_325_residues
MKAAVVHEWGQEPAYTDFPDPQPTGGAEVATVEASALTNLTRAVISGKHYSSKEITLPAIAGVDGVARLANGKRVYSGSL
APYGMMAERTVVYPAGAVEVPEHVDSVTAAAIPNPGISAWVALSHGAAVQPGQHVLVLGATGVTGSLAVQLAKSVFGAGH
VTVAGRNAERLDWLRTVGADDAIMLGSDDLTEAITARHATRPFDAVLDYLWGSPAEQTLSALAASHPSAHFHATRFVQIG
AMTGETISVNASTLRSTGITLSGVGIGSVPPEALQHARTEALPKLFAMVNNGDLQLQTLARPLADIETIWAAKEPSGVRV
VVTPQ

Specific function: Preferred Specificity Is Towards 1-Propanol. [C]

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.1.1.1 [C]

Molecular weight: Translated: 33681; Mature: 33681

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAAVVHEWGQEPAYTDFPDPQPTGGAEVATVEASALTNLTRAVISGKHYSSKEITLPAI
CCCCEEHHCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCCCEEEHHH
AGVDGVARLANGKRVYSGSLAPYGMMAERTVVYPAGAVEVPEHVDSVTAAAIPNPGISAW
CCCHHHHHHCCCCEEEECCCCCCCEECCCEEEECCCCCCCHHHHHHHHHHCCCCCCCEEE
VALSHGAAVQPGQHVLVLGATGVTGSLAVQLAKSVFGAGHVTVAGRNAERLDWLRTVGAD
EEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHCCCC
DAIMLGSDDLTEAITARHATRPFDAVLDYLWGSPAEQTLSALAASHPSAHFHATRFVQIG
CEEEECCCHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEEEEEE
AMTGETISVNASTLRSTGITLSGVGIGSVPPEALQHARTEALPKLFAMVNNGDLQLQTLA
CCCCCEEEEEHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEHHHHH
RPLADIETIWAAKEPSGVRVVVTPQ
HHHHHHHHHHCCCCCCCCEEEEECC
>Mature Secondary Structure
MKAAVVHEWGQEPAYTDFPDPQPTGGAEVATVEASALTNLTRAVISGKHYSSKEITLPAI
CCCCEEHHCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCCCEEEHHH
AGVDGVARLANGKRVYSGSLAPYGMMAERTVVYPAGAVEVPEHVDSVTAAAIPNPGISAW
CCCHHHHHHCCCCEEEECCCCCCCEECCCEEEECCCCCCCHHHHHHHHHHCCCCCCCEEE
VALSHGAAVQPGQHVLVLGATGVTGSLAVQLAKSVFGAGHVTVAGRNAERLDWLRTVGAD
EEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHCCCC
DAIMLGSDDLTEAITARHATRPFDAVLDYLWGSPAEQTLSALAASHPSAHFHATRFVQIG
CEEEECCCHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEEEEEE
AMTGETISVNASTLRSTGITLSGVGIGSVPPEALQHARTEALPKLFAMVNNGDLQLQTLA
CCCCCEEEEEHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEHHHHH
RPLADIETIWAAKEPSGVRVVVTPQ
HHHHHHHHHHCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+; NADH [C]

Metal ions: Co2+ Zn2+-depleted [C]

Kcat value (1/min): 150 [C]

Specific activity: 19

Km value (mM): 0.2 {tryptophol}} 0.0074 {NAD+}} 0.0069 {NAD+}} 13 {ethylene} 6 {methanol}} 27 {pentanol}} 0.44 {1-butanol}} 120 {ethanol}} 0.91 {acetaldehyde}} 0.1 {acetaldehyde}} 0.005 {1-octanol}} 0.005 {cyclohexanol}} 0.004 {

Substrates: D-Glyceraldehyde; H+; Nicotinamide adenine dinucleotide - reduced [C]

Specific reaction: D-Glyceraldehyde + H+ + Nicotinamide adenine dinucleotide - reduced <==> Glycerol + Nicotinamide adenine dinucleotide [C]

General reaction: Redox reaction [C]

Inhibitor: 2, 2'-Bipyridyl; 4-Methyl pyrazole acetaldehyde; 8-Amino-6-methoxyquinoline; 8-Hydroxy quinoline-5-sulfonic acid; Acetaldehyde; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA