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The map label for this gene is rutB [H]

Identifier: 169628940

GI number: 169628940

Start: 1850600

End: 1851235

Strand: Reverse

Name: rutB [H]

Synonym: MAB_1852c

Alternate gene names: 169628940

Gene position: 1851235-1850600 (Counterclockwise)

Preceding gene: 169628941

Following gene: 169628938

Centisome position: 36.53

GC content: 67.14

Gene sequence:

>636_bases
GTGACTGATTTTGTCGCTCCCGAGTGGGGCAGCTCAGCCCTGGTGATCATCGACGTGCAGACCGAGTTTGTCTCCGGCGC
GATGACAGTGCCGGGAACCGCGGACCGCATTCCAGCTCTCGCCCGTCTGGCCTCGGCGTTCCGTAAGGCGGGCCGGCCGA
TCGTGCATGTGGTGCGGCTGTACGTGCCCGGCGGTTCCGATACCGATCTGCCCCGTCGCGCGGAGATTCTCGCCGGACGC
GAGATCGCCGCACCGGGCACCGACGGATCACAGATTCCCCACGAGCTCCTGCCCACCGGCGCACAACTGGATTCCGCGCT
GCTGTTGGCGGGTGGGTTCCAGGAGGTCGGGCCCGCCGAACACATCCTGTTCAAGCCGCGCTGGAGCGCCTTCTACCGCA
CCGAACTGGAACAGCACCTACGTGAGCGCGGCATATCGACCGTGGTGGTCGCGGGCTGCAACCTGCCGAACTGCCCGCGC
GCCACGCTGTTCGACGCCTCCGAGCGGGACTACCGCGCCGTGGTCGTCGAGGACGCCACCTCGCAGGTGACGCCGGAGCG
GCTGCACGATCTGAGGCTCATCGGCGTGAACATCAGCGATGTTGCGTCGGTGGAAAAGGAGCTGACGGGAGCCTAG

Upstream 100 bases:

>100_bases
TATTCGCGCGCGCCCTGGCCGGCGCAGAAGGTCAAGACGATTTCGACCTCGTCCTGCGCCACCTGGGCCGCTCCCCGGAT
TGGGCACCTACTGTTGTGGG

Downstream 100 bases:

>100_bases
GAGCGCCCCGGGTTTCCGGGGCATACACCTAGGTGCCCGCGTCTATGCGACAACTCGGCGGGCACGCCGGTTTGTCGCAT
AGAGGCGGGCAACTCGGACT

Product: isochorismatase hydrolase

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MTDFVAPEWGSSALVIIDVQTEFVSGAMTVPGTADRIPALARLASAFRKAGRPIVHVVRLYVPGGSDTDLPRRAEILAGR
EIAAPGTDGSQIPHELLPTGAQLDSALLLAGGFQEVGPAEHILFKPRWSAFYRTELEQHLRERGISTVVVAGCNLPNCPR
ATLFDASERDYRAVVVEDATSQVTPERLHDLRLIGVNISDVASVEKELTGA

Sequences:

>Translated_211_residues
MTDFVAPEWGSSALVIIDVQTEFVSGAMTVPGTADRIPALARLASAFRKAGRPIVHVVRLYVPGGSDTDLPRRAEILAGR
EIAAPGTDGSQIPHELLPTGAQLDSALLLAGGFQEVGPAEHILFKPRWSAFYRTELEQHLRERGISTVVVAGCNLPNCPR
ATLFDASERDYRAVVVEDATSQVTPERLHDLRLIGVNISDVASVEKELTGA
>Mature_210_residues
TDFVAPEWGSSALVIIDVQTEFVSGAMTVPGTADRIPALARLASAFRKAGRPIVHVVRLYVPGGSDTDLPRRAEILAGRE
IAAPGTDGSQIPHELLPTGAQLDSALLLAGGFQEVGPAEHILFKPRWSAFYRTELEQHLRERGISTVVVAGCNLPNCPRA
TLFDASERDYRAVVVEDATSQVTPERLHDLRLIGVNISDVASVEKELTGA

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081992, Length=201, Percent_Identity=32.8358208955224, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 22690; Mature: 22559

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDFVAPEWGSSALVIIDVQTEFVSGAMTVPGTADRIPALARLASAFRKAGRPIVHVVRL
CCCCCCCCCCCCEEEEEEECHHHHCCCEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEEE
YVPGGSDTDLPRRAEILAGREIAAPGTDGSQIPHELLPTGAQLDSALLLAGGFQEVGPAE
EECCCCCCCCCHHHHHHCCCEECCCCCCCCCCCHHHCCCCCCCCCCCEEECCCHHCCCCH
HILFKPRWSAFYRTELEQHLRERGISTVVVAGCNLPNCPRATLFDASERDYRAVVVEDAT
HEEECCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCEEEEEEECCC
SQVTPERLHDLRLIGVNISDVASVEKELTGA
CCCCHHHHHCEEEEECCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDFVAPEWGSSALVIIDVQTEFVSGAMTVPGTADRIPALARLASAFRKAGRPIVHVVRL
CCCCCCCCCCCEEEEEEECHHHHCCCEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEEE
YVPGGSDTDLPRRAEILAGREIAAPGTDGSQIPHELLPTGAQLDSALLLAGGFQEVGPAE
EECCCCCCCCCHHHHHHCCCEECCCCCCCCCCCHHHCCCCCCCCCCCEEECCCHHCCCCH
HILFKPRWSAFYRTELEQHLRERGISTVVVAGCNLPNCPRATLFDASERDYRAVVVEDAT
HEEECCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCEEEEEEECCC
SQVTPERLHDLRLIGVNISDVASVEKELTGA
CCCCHHHHHCEEEEECCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA