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The map label for this gene is fadD26 [H]

Identifier: 169628580

GI number: 169628580

Start: 1498630

End: 1500426

Strand: Direct

Name: fadD26 [H]

Synonym: MAB_1489

Alternate gene names: 169628580

Gene position: 1498630-1500426 (Clockwise)

Preceding gene: 169628576

Following gene: 169628581

Centisome position: 29.58

GC content: 64.77

Gene sequence:

>1797_bases
GTGGTGCGCAGTGACGGTTTGCTTGACATCGACGATTGCCTGAATGCCGACGGGAATATCGAGCTACCGCCCGGAACCAC
GCTGATATCCCTGATCGACCGGAACATCAGGAACGTCGGGGACATGGTCGCCTACCGCTATCTGGACTTCACCGCTTCGG
AGGACGGGCATGCCATTGAACTCACCTGGTCGCAGCTCGGCATCCGTCTGAGCGCCGTCGCCGCGCAGCTGCAACGCCAC
GTCACGCGTGGCGAGCGGGTGGCCATTTTGGCGCCGCAGGGGCTCGACTACATCGTCGGCTTCTTCTCGGCGATCAAGGC
CGGCGCCATCGCGGTGCCGCTGTTCGCTCCCGAGCTACCGGGTCACGCCGAGCGGCTGGATATCGCGCTGAGGGACTGCA
CGCCCACCGTGGTCCTGACGACGGCCGCCGTGCGCGACACTGTCGAAGAGTTTCTCGATGGGCGGCCGGAGGCGCCACTC
GTGCTCGCCATCGACGAGATCCCCGACGAAGCAGGGGAGGGCTTCGAGTACGTGCACCTTGTCGATGACGACGTCTCACA
TCTGCAATACACTTCCGGTGCCACCCGGCCCCCAGTGGGCGTGGAAATCACCCACCGGGCGGTCGGCGTCAATCTGACCC
AGATGATTCTGTCGATCAATCTGCTGGATCGAAACACGCACGGCTGCAGCTGGTTACCGCTTTACCACGATATGGGTCTG
TCGATGATCGGCTTCCCAACCGTCTACGGCGGGCACTCCACCTTGATGTCACCCACCGCTTTCATCCGCCGCCCGCAGCG
CTGGATCAAGGCACTCTCCGACGGCTGCACAGAAGGTCGGGTGATCACCGCGGCACCCAACTTCGCCTACGAGTACACCG
CGCAACGCGGGGTCCCGAAGGACGGTGCGGGTATCGACCTGGGCAAGGCCGTGATGATCATCGGCTCCGAGCCGGTCAGC
ATCGACGCCATCCGTAACTTCGAAAAGGCTTTTGCCCCCTTCGGTTTACCGTCGACAGCGTTCAAGCCGTCCTACGGTAT
CGCCGAGGCGACACTGTTCATCGCCAACATCGCCCCGGACGCCGAGCCTTCCGTGGCATATCTCGATCGCACCCAGCTCG
CCCGAGGTCGCGCGGTGCCGACAGATCCGGATACGCCCCATGTCAGCGTCCACGTTTCCTGCGGTCAGCTGGCACGCAGT
CTGCACGGGGTGATCGTCGATCCCGTCGGCACCGATGAGCTCCCAGACGGTCACGTCGGCGAAATATGGTTGCAAGGCAA
CAATATTGGGCGCGGATACTGGGGTCGCCCGGAGGACACCGAGAAGGTCTTTCATGCACGGCTTGGCGCAAGACAACCGA
AAGGCCATGCGGGCGAGGCCGATATCGAGGGCGACTGGCTGCGCACGGGCGACATGGGCTTCTATCTCGACGGTGAGTTG
TACGTCACCGGCCGCCTCGCCGACCACATCGAGGTCGACGGCAGCAGCCACTATCCGCAGGACATCGAGGCCAGCGTGGC
AGCCGCCTCGCCTATCGTGCGGCGCGGTTATGTGACCGCGTTCACGGTGCCCGGGGGCGGACTGGTCATCGTCGCCGAGC
GCGCCTCACGCACGGCCAAGGCCGACCCGCAGGAGGCCATCGATGCGATCAGCGCCGCGGTCGATGCGGAATATGGGTTG
AAGCCCGCCGACGTGCTGCTTCTGTCGGCGGGGGCGATCCCGCGCACTACCAGTGGAAAGCTGGCACGGCGGGCCTGTCG
CCGCCACTACCTGGAGGGCACGCTGGGCGTCCACTGA

Upstream 100 bases:

>100_bases
TCGAAATCGACGTTCTGCCGACAACGTGCGAGTGAGCCTCTGCGAAACGTAGATCTCGGCGATCTCGGCGCCCGTCGCCG
CCGCGCCTAGACTTGAAAAC

Downstream 100 bases:

>100_bases
GATCCCAGCTAACTCATTTGCCTCATTTGTCCCACGTCAGCGAGCCCATTCGCCACACTCCCGTCGGCCCGGTAGGTTTT
CCGGGCCAGTGGCTACGACG

Product: putative fatty-acid--CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 598; Mature: 598

Protein sequence:

>598_residues
MVRSDGLLDIDDCLNADGNIELPPGTTLISLIDRNIRNVGDMVAYRYLDFTASEDGHAIELTWSQLGIRLSAVAAQLQRH
VTRGERVAILAPQGLDYIVGFFSAIKAGAIAVPLFAPELPGHAERLDIALRDCTPTVVLTTAAVRDTVEEFLDGRPEAPL
VLAIDEIPDEAGEGFEYVHLVDDDVSHLQYTSGATRPPVGVEITHRAVGVNLTQMILSINLLDRNTHGCSWLPLYHDMGL
SMIGFPTVYGGHSTLMSPTAFIRRPQRWIKALSDGCTEGRVITAAPNFAYEYTAQRGVPKDGAGIDLGKAVMIIGSEPVS
IDAIRNFEKAFAPFGLPSTAFKPSYGIAEATLFIANIAPDAEPSVAYLDRTQLARGRAVPTDPDTPHVSVHVSCGQLARS
LHGVIVDPVGTDELPDGHVGEIWLQGNNIGRGYWGRPEDTEKVFHARLGARQPKGHAGEADIEGDWLRTGDMGFYLDGEL
YVTGRLADHIEVDGSSHYPQDIEASVAAASPIVRRGYVTAFTVPGGGLVIVAERASRTAKADPQEAIDAISAAVDAEYGL
KPADVLLLSAGAIPRTTSGKLARRACRRHYLEGTLGVH

Sequences:

>Translated_598_residues
MVRSDGLLDIDDCLNADGNIELPPGTTLISLIDRNIRNVGDMVAYRYLDFTASEDGHAIELTWSQLGIRLSAVAAQLQRH
VTRGERVAILAPQGLDYIVGFFSAIKAGAIAVPLFAPELPGHAERLDIALRDCTPTVVLTTAAVRDTVEEFLDGRPEAPL
VLAIDEIPDEAGEGFEYVHLVDDDVSHLQYTSGATRPPVGVEITHRAVGVNLTQMILSINLLDRNTHGCSWLPLYHDMGL
SMIGFPTVYGGHSTLMSPTAFIRRPQRWIKALSDGCTEGRVITAAPNFAYEYTAQRGVPKDGAGIDLGKAVMIIGSEPVS
IDAIRNFEKAFAPFGLPSTAFKPSYGIAEATLFIANIAPDAEPSVAYLDRTQLARGRAVPTDPDTPHVSVHVSCGQLARS
LHGVIVDPVGTDELPDGHVGEIWLQGNNIGRGYWGRPEDTEKVFHARLGARQPKGHAGEADIEGDWLRTGDMGFYLDGEL
YVTGRLADHIEVDGSSHYPQDIEASVAAASPIVRRGYVTAFTVPGGGLVIVAERASRTAKADPQEAIDAISAAVDAEYGL
KPADVLLLSAGAIPRTTSGKLARRACRRHYLEGTLGVH
>Mature_598_residues
MVRSDGLLDIDDCLNADGNIELPPGTTLISLIDRNIRNVGDMVAYRYLDFTASEDGHAIELTWSQLGIRLSAVAAQLQRH
VTRGERVAILAPQGLDYIVGFFSAIKAGAIAVPLFAPELPGHAERLDIALRDCTPTVVLTTAAVRDTVEEFLDGRPEAPL
VLAIDEIPDEAGEGFEYVHLVDDDVSHLQYTSGATRPPVGVEITHRAVGVNLTQMILSINLLDRNTHGCSWLPLYHDMGL
SMIGFPTVYGGHSTLMSPTAFIRRPQRWIKALSDGCTEGRVITAAPNFAYEYTAQRGVPKDGAGIDLGKAVMIIGSEPVS
IDAIRNFEKAFAPFGLPSTAFKPSYGIAEATLFIANIAPDAEPSVAYLDRTQLARGRAVPTDPDTPHVSVHVSCGQLARS
LHGVIVDPVGTDELPDGHVGEIWLQGNNIGRGYWGRPEDTEKVFHARLGARQPKGHAGEADIEGDWLRTGDMGFYLDGEL
YVTGRLADHIEVDGSSHYPQDIEASVAAASPIVRRGYVTAFTVPGGGLVIVAERASRTAKADPQEAIDAISAAVDAEYGL
KPADVLLLSAGAIPRTTSGKLARRACRRHYLEGTLGVH

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=575, Percent_Identity=24.695652173913, Blast_Score=118, Evalue=2e-26,
Organism=Homo sapiens, GI225735629, Length=507, Percent_Identity=26.232741617357, Blast_Score=113, Evalue=5e-25,
Organism=Homo sapiens, GI45827692, Length=507, Percent_Identity=26.232741617357, Blast_Score=113, Evalue=6e-25,
Organism=Homo sapiens, GI55749758, Length=522, Percent_Identity=23.9463601532567, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI225735625, Length=568, Percent_Identity=23.0633802816901, Blast_Score=77, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI193202679, Length=629, Percent_Identity=22.5755166931638, Blast_Score=108, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17559526, Length=609, Percent_Identity=24.6305418719212, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24654656, Length=456, Percent_Identity=24.5614035087719, Blast_Score=108, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 64236; Mature: 64236

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRSDGLLDIDDCLNADGNIELPPGTTLISLIDRNIRNVGDMVAYRYLDFTASEDGHAIE
CCCCCCCEEHHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEE
LTWSQLGIRLSAVAAQLQRHVTRGERVAILAPQGLDYIVGFFSAIKAGAIAVPLFAPELP
EEHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCC
GHAERLDIALRDCTPTVVLTTAAVRDTVEEFLDGRPEAPLVLAIDEIPDEAGEGFEYVHL
CCHHHEEEEEECCCCEEEEEHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEEEEE
VDDDVSHLQYTSGATRPPVGVEITHRAVGVNLTQMILSINLLDRNTHGCSWLPLYHDMGL
ECCCHHHEEECCCCCCCCCCEEEECHHHCCCHHHHHHEEEEEECCCCCEEEEEHHHHCCC
SMIGFPTVYGGHSTLMSPTAFIRRPQRWIKALSDGCTEGRVITAAPNFAYEYTAQRGVPK
CEECCCEEECCCHHHCCCHHHHHCHHHHHHHHHCCCCCCEEEEECCCCEEEEHHHCCCCC
DGAGIDLGKAVMIIGSEPVSIDAIRNFEKAFAPFGLPSTAFKPSYGIAEATLFIANIAPD
CCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCC
AEPSVAYLDRTQLARGRAVPTDPDTPHVSVHVSCGQLARSLHGVIVDPVGTDELPDGHVG
CCCCEEEHHHHHHHCCCCCCCCCCCCEEEEEEEHHHHHHHHCCEEECCCCCCCCCCCCCE
EIWLQGNNIGRGYWGRPEDTEKVFHARLGARQPKGHAGEADIEGDWLRTGDMGFYLDGEL
EEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCEEECCEE
YVTGRLADHIEVDGSSHYPQDIEASVAAASPIVRRGYVTAFTVPGGGLVIVAERASRTAK
EEEEEECCEEEECCCCCCCCCHHHHHHHHCCHHHCCEEEEEEECCCCEEEEEECCCCCCC
ADPQEAIDAISAAVDAEYGLKPADVLLLSAGAIPRTTSGKLARRACRRHYLEGTLGVH
CCHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MVRSDGLLDIDDCLNADGNIELPPGTTLISLIDRNIRNVGDMVAYRYLDFTASEDGHAIE
CCCCCCCEEHHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEE
LTWSQLGIRLSAVAAQLQRHVTRGERVAILAPQGLDYIVGFFSAIKAGAIAVPLFAPELP
EEHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCC
GHAERLDIALRDCTPTVVLTTAAVRDTVEEFLDGRPEAPLVLAIDEIPDEAGEGFEYVHL
CCHHHEEEEEECCCCEEEEEHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEEEEE
VDDDVSHLQYTSGATRPPVGVEITHRAVGVNLTQMILSINLLDRNTHGCSWLPLYHDMGL
ECCCHHHEEECCCCCCCCCCEEEECHHHCCCHHHHHHEEEEEECCCCCEEEEEHHHHCCC
SMIGFPTVYGGHSTLMSPTAFIRRPQRWIKALSDGCTEGRVITAAPNFAYEYTAQRGVPK
CEECCCEEECCCHHHCCCHHHHHCHHHHHHHHHCCCCCCEEEEECCCCEEEEHHHCCCCC
DGAGIDLGKAVMIIGSEPVSIDAIRNFEKAFAPFGLPSTAFKPSYGIAEATLFIANIAPD
CCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCC
AEPSVAYLDRTQLARGRAVPTDPDTPHVSVHVSCGQLARSLHGVIVDPVGTDELPDGHVG
CCCCEEEHHHHHHHCCCCCCCCCCCCEEEEEEEHHHHHHHHCCEEECCCCCCCCCCCCCE
EIWLQGNNIGRGYWGRPEDTEKVFHARLGARQPKGHAGEADIEGDWLRTGDMGFYLDGEL
EEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCEEECCEE
YVTGRLADHIEVDGSSHYPQDIEASVAAASPIVRRGYVTAFTVPGGGLVIVAERASRTAK
EEEEEECCEEEECCCCCCCCCHHHHHHHHCCHHHCCEEEEEEECCCCEEEEEECCCCCCC
ADPQEAIDAISAAVDAEYGLKPADVLLLSAGAIPRTTSGKLARRACRRHYLEGTLGVH
CCHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]