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The map label for this gene is rph

Identifier: 169628575

GI number: 169628575

Start: 1495025

End: 1495801

Strand: Direct

Name: rph

Synonym: MAB_1484

Alternate gene names: 169628575

Gene position: 1495025-1495801 (Clockwise)

Preceding gene: 169628574

Following gene: 169628576

Centisome position: 29.5

GC content: 65.25

Gene sequence:

>777_bases
GTGTCCAGACGAGAAGACGGCAGACAAGACGACGAATTGCGCCCCGTGACCATCACACGGGGATTCACTTCGCATCCAGC
GGGTTCGGTACTGATCGAGTTCGGTCAAACGCGGGTGATGTGCACTGCCAGCGCCACCGAGGGGGTGCCACGCTGGCGCA
AGGGTTCTGGACTTGGTTGGCTGACAGCCGAATACGCGATGCTGCCGGCTTCCACGCACACCCGTAGCGATCGGGAATCG
GTCAAGGGGCGTGTCGGCGGCCGCACCCAGGAGATCAGCCGTCTGATCGGGCGCTCGCTGCGGGCCTGTATCGATTTGTC
GGCGTTGGGGGAGAACACCATTGCCATCGACTGTGATGTGTTGCAGGCCGACGGTGGCACGCGCACGGCCGCGATCACCG
GGGCCTATGTGGCCCTGGCCGATGCGGTGACCTACCTGGCCGCGCACGGCAAGCTGGCAGATGACGAACCGCTCTCGTGT
GCGATTGCCGCCGTGAGCGTCGGCGTGGTGGATGGCCGGGTGCGCGTCGACCTTCCATATGAAGAGGATTCGCGTGCCGA
GGTGGATATGAACGTGGTCGCCACCGATACCGGCACCCTGGTGGAGGTGCAAGGCACCGGTGAGGGCGCGACCTTTGCGC
GGTCAACCCTGGACAAGCTGCTCGATGCCGCGGTGGGCGCCGCCGACCAGCTGTTCGTGCTGCAGAAAGAAGCGCTCGCG
TTGCCGTACCCCGGGGTACTGCCCGACGCGCCGCAGAAGAAGGCATTCGGTTCGTAG

Upstream 100 bases:

>100_bases
TTCGACGGACCGGTACATGCCGTGGTCGCCGGGGAGAACATCGACCTGTAGCTCCGTCACTCACGCTCGGCACCGTCGCG
GAGACGATAAGGTCGAAGGC

Downstream 100 bases:

>100_bases
TAGGCCGGGTTCCATGAAAATACTTGTTGCAAGCCGTAATCCGAAGAAGCTCGCGGAGCTGTCGCGAGTGCTCGAGTCGT
CGGGCGTCTCCGGCGTCGAG

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MSRREDGRQDDELRPVTITRGFTSHPAGSVLIEFGQTRVMCTASATEGVPRWRKGSGLGWLTAEYAMLPASTHTRSDRES
VKGRVGGRTQEISRLIGRSLRACIDLSALGENTIAIDCDVLQADGGTRTAAITGAYVALADAVTYLAAHGKLADDEPLSC
AIAAVSVGVVDGRVRVDLPYEEDSRAEVDMNVVATDTGTLVEVQGTGEGATFARSTLDKLLDAAVGAADQLFVLQKEALA
LPYPGVLPDAPQKKAFGS

Sequences:

>Translated_258_residues
MSRREDGRQDDELRPVTITRGFTSHPAGSVLIEFGQTRVMCTASATEGVPRWRKGSGLGWLTAEYAMLPASTHTRSDRES
VKGRVGGRTQEISRLIGRSLRACIDLSALGENTIAIDCDVLQADGGTRTAAITGAYVALADAVTYLAAHGKLADDEPLSC
AIAAVSVGVVDGRVRVDLPYEEDSRAEVDMNVVATDTGTLVEVQGTGEGATFARSTLDKLLDAAVGAADQLFVLQKEALA
LPYPGVLPDAPQKKAFGS
>Mature_257_residues
SRREDGRQDDELRPVTITRGFTSHPAGSVLIEFGQTRVMCTASATEGVPRWRKGSGLGWLTAEYAMLPASTHTRSDRESV
KGRVGGRTQEISRLIGRSLRACIDLSALGENTIAIDCDVLQADGGTRTAAITGAYVALADAVTYLAAHGKLADDEPLSCA
IAAVSVGVVDGRVRVDLPYEEDSRAEVDMNVVATDTGTLVEVQGTGEGATFARSTLDKLLDAAVGAADQLFVLQKEALAL
PYPGVLPDAPQKKAFGS

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=209, Percent_Identity=62.200956937799, Blast_Score=263, Evalue=6e-72,
Organism=Caenorhabditis elegans, GI71981632, Length=214, Percent_Identity=28.0373831775701, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_MYCA9 (B1MLZ3)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001702224.1
- ProteinModelPortal:   B1MLZ3
- SMR:   B1MLZ3
- EnsemblBacteria:   EBMYCT00000005200
- GeneID:   5964005
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_1484
- GeneTree:   EBGT00050000017525
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- ProtClustDB:   PRK00173
- BioCyc:   MABS561007:MAB_1484-MONOMER
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 27206; Mature: 27075

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRREDGRQDDELRPVTITRGFTSHPAGSVLIEFGQTRVMCTASATEGVPRWRKGSGLGW
CCCCCCCCCCCCCCCEEEECCCCCCCCCEEEEEECCEEEEEEECCCCCCCCCCCCCCCEE
LTAEYAMLPASTHTRSDRESVKGRVGGRTQEISRLIGRSLRACIDLSALGENTIAIDCDV
EEEEEEECCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
LQADGGTRTAAITGAYVALADAVTYLAAHGKLADDEPLSCAIAAVSVGVVDGRVRVDLPY
EECCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEECCEEEEECCC
EEDSRAEVDMNVVATDTGTLVEVQGTGEGATFARSTLDKLLDAAVGAADQLFVLQKEALA
CCCCCCEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHEEEEEHHHHC
LPYPGVLPDAPQKKAFGS
CCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SRREDGRQDDELRPVTITRGFTSHPAGSVLIEFGQTRVMCTASATEGVPRWRKGSGLGW
CCCCCCCCCCCCCCEEEECCCCCCCCCEEEEEECCEEEEEEECCCCCCCCCCCCCCCEE
LTAEYAMLPASTHTRSDRESVKGRVGGRTQEISRLIGRSLRACIDLSALGENTIAIDCDV
EEEEEEECCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
LQADGGTRTAAITGAYVALADAVTYLAAHGKLADDEPLSCAIAAVSVGVVDGRVRVDLPY
EECCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEECCEEEEECCC
EEDSRAEVDMNVVATDTGTLVEVQGTGEGATFARSTLDKLLDAAVGAADQLFVLQKEALA
CCCCCCEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHEEEEEHHHHC
LPYPGVLPDAPQKKAFGS
CCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA