BacMap

Definition	Mycobacterium abscessus ATCC 19977 chromosome chromosome 1, complete sequence.
Accession	NC_010397
Length	5,067,172

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The map label for this gene is lcfB [H]

Identifier: 169628425

GI number: 169628425

Start: 1333982

End: 1335373

Strand: Direct

Name: lcfB [H]

Synonym: MAB_1332

Alternate gene names: 169628425

Gene position: 1333982-1335373 (Clockwise)

Preceding gene: 169628424

Following gene: 169628426

Centisome position: 26.33

GC content: 65.73

Gene sequence:

>1392_bases
ATGCTGCTTACGTCGCTTCACCCCAAGTCGCCTGATCTAGCGGACGCGGTCCGTATCGGTGAGGACAGCTGGGGGCGCGG
TGACCTCGTGGGTGCTGCGACGGCCGCCGCCGAGCGCATCGGCGGGGCTTCAGGACCGATCGCGGTGCTCGCCCGCCCCA
GCATCGACACGGTTCTGGCGGTGGTCGCCGGACTCATCGCGGGCGTGCCGATCGTCCCGGTACCCGCAGACGTCGGGATT
GCCGAACGACGCCACATTCTGGCCGACAGCGCCGCGGTGGCCTGGCTGGGTGAATCCCCGGACGAGTCAGAGGGATTGCC
CCACGTGCCGGTGCGCCGGCACGCACGCTCATGGCATCAATATTCCGAACCGAATCCCGAGTCGACTGCGCTCATCGTGT
ATACCTCGGGAACCACCGGTGCACCGAAGGGCGTGCGACTGAGCCGGCGTGCGCTCGCTGCCAGCCTCGACGGTCTTGCG
GACGCGTGGGAATGGACTGCCGACGACGTGCTGGTGCATGGTCTTCCGCTATTTCACGTGCACGGGCTGGTGCTCGGCCT
GCTGGGTTCATTGCGAGTGGGTAATCGGTTCGTGCATACCGTCAAGCCCACACCAGAGGCCTACGCGGCGGCACGAGGCA
GCCTGTACTTCGGCGTGCCGACGGTATGGTTGCGGGTGGCCGGCGATGAATCCTGTGCGCGCGCACTGTCTACCGCGCGC
CTGCTGGTATCCGGCAGTGCGCCACTGCCGGTGCCTGTGTTCGACGAGCTATGCCGACTTACCGGGCATGCGCCCATCGA
GCGCTATGGGGCCACCGAAACCGTCATCACACTTTCCACCCGTGCCGATGGAGAACGGCGCGCCGGCTGGGTTGGCCACC
CGCTGACCGGTGTCCGGACCCGTCTGCTGGCAGAGAATGGGTCTCCGGCCGCCCACGACGGGGAAACCGTGGGCCAGCTG
CAGATTCAGGGCCCCACATTATTCAGTGGCTATCACAACCTGCCCGAGAAGACGGCCGAGGTACTCACGGAGGATGGCTG
GTACCGCACCGGTGATGTCGCCGTCATCGATGCCGGGGGAATGCACCGCATCGTCGGCCGCGAATCGGTCGACCTCATCA
AGTCCGGCGGCTACCGAATCGGCGCCGGTGAAATCGAGACCGTGCTGCTAGGGCATCCAGGCATCGTGGAAGTTGCGGTG
GTGGGGGTCCCCGATGACGACCTGGGACAACGGATCGTCGCCTACGTTGTCGGTGATGTGGCAGAAGATGACGTGATCGG
CTTCGTGGCGCAGCAGCTTTCGGTGCACAAGCGTCCCCGTGAGGTTCGGCGGGTCGACGCGTTACCCCGGAACGCGATGG
GCAAGGTCCTCAAGAAGGAGTTGATCACATGA

Upstream 100 bases:

>100_bases
GTGGTACGGATACTTCGTGCGTCGTATCGCCGAGCGCCCCGCCAACATGACGTTCTTCCTTCGCGCACTCGTTTCCGGAG
GCTAGCTCTAGGCTGGCTTC

Downstream 100 bases:

>100_bases
AGGTGGCCGCAGAGGTCGTCGACCAGATCGACTGGCACTGGACGTCTCAGCTGCGGCCCCGGTTCGATGGTCTGACCGAT
GATGAGTATTTCTGGGAACC

Product: acyl-CoA synthetase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MLLTSLHPKSPDLADAVRIGEDSWGRGDLVGAATAAAERIGGASGPIAVLARPSIDTVLAVVAGLIAGVPIVPVPADVGI
AERRHILADSAAVAWLGESPDESEGLPHVPVRRHARSWHQYSEPNPESTALIVYTSGTTGAPKGVRLSRRALAASLDGLA
DAWEWTADDVLVHGLPLFHVHGLVLGLLGSLRVGNRFVHTVKPTPEAYAAARGSLYFGVPTVWLRVAGDESCARALSTAR
LLVSGSAPLPVPVFDELCRLTGHAPIERYGATETVITLSTRADGERRAGWVGHPLTGVRTRLLAENGSPAAHDGETVGQL
QIQGPTLFSGYHNLPEKTAEVLTEDGWYRTGDVAVIDAGGMHRIVGRESVDLIKSGGYRIGAGEIETVLLGHPGIVEVAV
VGVPDDDLGQRIVAYVVGDVAEDDVIGFVAQQLSVHKRPREVRRVDALPRNAMGKVLKKELIT

Sequences:

>Translated_463_residues
MLLTSLHPKSPDLADAVRIGEDSWGRGDLVGAATAAAERIGGASGPIAVLARPSIDTVLAVVAGLIAGVPIVPVPADVGI
AERRHILADSAAVAWLGESPDESEGLPHVPVRRHARSWHQYSEPNPESTALIVYTSGTTGAPKGVRLSRRALAASLDGLA
DAWEWTADDVLVHGLPLFHVHGLVLGLLGSLRVGNRFVHTVKPTPEAYAAARGSLYFGVPTVWLRVAGDESCARALSTAR
LLVSGSAPLPVPVFDELCRLTGHAPIERYGATETVITLSTRADGERRAGWVGHPLTGVRTRLLAENGSPAAHDGETVGQL
QIQGPTLFSGYHNLPEKTAEVLTEDGWYRTGDVAVIDAGGMHRIVGRESVDLIKSGGYRIGAGEIETVLLGHPGIVEVAV
VGVPDDDLGQRIVAYVVGDVAEDDVIGFVAQQLSVHKRPREVRRVDALPRNAMGKVLKKELIT
>Mature_463_residues
MLLTSLHPKSPDLADAVRIGEDSWGRGDLVGAATAAAERIGGASGPIAVLARPSIDTVLAVVAGLIAGVPIVPVPADVGI
AERRHILADSAAVAWLGESPDESEGLPHVPVRRHARSWHQYSEPNPESTALIVYTSGTTGAPKGVRLSRRALAASLDGLA
DAWEWTADDVLVHGLPLFHVHGLVLGLLGSLRVGNRFVHTVKPTPEAYAAARGSLYFGVPTVWLRVAGDESCARALSTAR
LLVSGSAPLPVPVFDELCRLTGHAPIERYGATETVITLSTRADGERRAGWVGHPLTGVRTRLLAENGSPAAHDGETVGQL
QIQGPTLFSGYHNLPEKTAEVLTEDGWYRTGDVAVIDAGGMHRIVGRESVDLIKSGGYRIGAGEIETVLLGHPGIVEVAV
VGVPDDDLGQRIVAYVVGDVAEDDVIGFVAQQLSVHKRPREVRRVDALPRNAMGKVLKKELIT

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=375, Percent_Identity=38.4, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI187761343, Length=375, Percent_Identity=38.4, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI156151445, Length=349, Percent_Identity=30.3724928366762, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI122937307, Length=383, Percent_Identity=27.6762402088773, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI115511026, Length=372, Percent_Identity=28.494623655914, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI40807491, Length=346, Percent_Identity=28.3236994219653, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI157311624, Length=443, Percent_Identity=25.7336343115124, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI157311622, Length=443, Percent_Identity=25.7336343115124, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI42544132, Length=368, Percent_Identity=26.3586956521739, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI58082049, Length=443, Percent_Identity=25.5079006772009, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI38505220, Length=409, Percent_Identity=25.1833740831296, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI57165412, Length=345, Percent_Identity=26.0869565217391, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI57165410, Length=345, Percent_Identity=25.7971014492754, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI42794756, Length=384, Percent_Identity=25.78125, Blast_Score=90, Evalue=6e-18,
Organism=Homo sapiens, GI42794760, Length=386, Percent_Identity=25.9067357512953, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI42794758, Length=386, Percent_Identity=25.9067357512953, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI28416953, Length=377, Percent_Identity=27.0557029177719, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI1788107, Length=359, Percent_Identity=30.9192200557103, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI145693145, Length=347, Percent_Identity=31.1239193083574, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI221142682, Length=356, Percent_Identity=27.8089887640449, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1786810, Length=355, Percent_Identity=28.7323943661972, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789201, Length=351, Percent_Identity=28.2051282051282, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790505, Length=379, Percent_Identity=29.023746701847, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786529, Length=379, Percent_Identity=30.6068601583113, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1788595, Length=329, Percent_Identity=28.2674772036474, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17558820, Length=476, Percent_Identity=34.453781512605, Blast_Score=218, Evalue=5e-57,
Organism=Caenorhabditis elegans, GI32563687, Length=454, Percent_Identity=31.7180616740088, Blast_Score=188, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI71994694, Length=364, Percent_Identity=29.1208791208791, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI71994703, Length=364, Percent_Identity=29.1208791208791, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI71994690, Length=364, Percent_Identity=29.1208791208791, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17531443, Length=352, Percent_Identity=29.2613636363636, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17559526, Length=388, Percent_Identity=29.1237113402062, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17557194, Length=368, Percent_Identity=27.9891304347826, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17538037, Length=364, Percent_Identity=29.6703296703297, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17560308, Length=358, Percent_Identity=28.4916201117318, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17556552, Length=348, Percent_Identity=29.0229885057471, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71996755, Length=364, Percent_Identity=28.2967032967033, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17560140, Length=343, Percent_Identity=26.530612244898, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI71985884, Length=360, Percent_Identity=25.2777777777778, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17510401, Length=180, Percent_Identity=27.7777777777778, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=343, Percent_Identity=30.6122448979592, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6320852, Length=403, Percent_Identity=27.7915632754342, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319264, Length=386, Percent_Identity=24.6113989637306, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24581924, Length=357, Percent_Identity=30.812324929972, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI21355181, Length=347, Percent_Identity=31.700288184438, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI18859661, Length=357, Percent_Identity=28.0112044817927, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI221329755, Length=396, Percent_Identity=24.4949494949495, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24656500, Length=354, Percent_Identity=30.225988700565, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19922652, Length=290, Percent_Identity=30.3448275862069, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24653035, Length=348, Percent_Identity=25.8620689655172, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI281366413, Length=389, Percent_Identity=25.1928020565553, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24666501, Length=389, Percent_Identity=25.1928020565553, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24666497, Length=389, Percent_Identity=25.1928020565553, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI62471689, Length=383, Percent_Identity=25.8485639686684, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI62471681, Length=383, Percent_Identity=25.8485639686684, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI62471687, Length=383, Percent_Identity=25.8485639686684, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24586634, Length=383, Percent_Identity=25.8485639686684, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI22026970, Length=383, Percent_Identity=25.8485639686684, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI62471679, Length=383, Percent_Identity=25.8485639686684, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI62471683, Length=383, Percent_Identity=25.8485639686684, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI62471685, Length=383, Percent_Identity=25.8485639686684, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24586636, Length=383, Percent_Identity=25.8485639686684, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24648260, Length=345, Percent_Identity=26.9565217391304, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI21356947, Length=357, Percent_Identity=23.249299719888, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI161076582, Length=355, Percent_Identity=24.7887323943662, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21356441, Length=323, Percent_Identity=24.4582043343653, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI21358303, Length=331, Percent_Identity=21.4501510574018, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI20130357, Length=350, Percent_Identity=26.8571428571429, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24648253, Length=354, Percent_Identity=23.1638418079096, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648255, Length=354, Percent_Identity=23.728813559322, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281365686, Length=318, Percent_Identity=21.0691823899371, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24648257, Length=347, Percent_Identity=25.0720461095101, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 48911; Mature: 48911

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLTSLHPKSPDLADAVRIGEDSWGRGDLVGAATAAAERIGGASGPIAVLARPSIDTVLA
CCCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHH
VVAGLIAGVPIVPVPADVGIAERRHILADSAAVAWLGESPDESEGLPHVPVRRHARSWHQ
HHHHHHCCCCCEECCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHH
YSEPNPESTALIVYTSGTTGAPKGVRLSRRALAASLDGLADAWEWTADDVLVHGLPLFHV
CCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHH
HGLVLGLLGSLRVGNRFVHTVKPTPEAYAAARGSLYFGVPTVWLRVAGDESCARALSTAR
HHHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCCEEECCCEEEEEECCCHHHHHHHHHHH
LLVSGSAPLPVPVFDELCRLTGHAPIERYGATETVITLSTRADGERRAGWVGHPLTGVRT
HEEECCCCCCCHHHHHHHHHCCCCCHHHCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHH
RLLAENGSPAAHDGETVGQLQIQGPTLFSGYHNLPEKTAEVLTEDGWYRTGDVAVIDAGG
HHHHCCCCCCCCCCCCEEEEEECCCEEECCCCCCHHHHHHHHHCCCCEEECCEEEECCCC
MHRIVGRESVDLIKSGGYRIGAGEIETVLLGHPGIVEVAVVGVPDDDLGQRIVAYVVGDV
CEEHHCCCHHHHHHCCCEEEECCCEEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHHCC
AEDDVIGFVAQQLSVHKRPREVRRVDALPRNAMGKVLKKELIT
CCHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MLLTSLHPKSPDLADAVRIGEDSWGRGDLVGAATAAAERIGGASGPIAVLARPSIDTVLA
CCCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHH
VVAGLIAGVPIVPVPADVGIAERRHILADSAAVAWLGESPDESEGLPHVPVRRHARSWHQ
HHHHHHCCCCCEECCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHH
YSEPNPESTALIVYTSGTTGAPKGVRLSRRALAASLDGLADAWEWTADDVLVHGLPLFHV
CCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHH
HGLVLGLLGSLRVGNRFVHTVKPTPEAYAAARGSLYFGVPTVWLRVAGDESCARALSTAR
HHHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCCEEECCCEEEEEECCCHHHHHHHHHHH
LLVSGSAPLPVPVFDELCRLTGHAPIERYGATETVITLSTRADGERRAGWVGHPLTGVRT
HEEECCCCCCCHHHHHHHHHCCCCCHHHCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHH
RLLAENGSPAAHDGETVGQLQIQGPTLFSGYHNLPEKTAEVLTEDGWYRTGDVAVIDAGG
HHHHCCCCCCCCCCCCEEEEEECCCEEECCCCCCHHHHHHHHHCCCCEEECCEEEECCCC
MHRIVGRESVDLIKSGGYRIGAGEIETVLLGHPGIVEVAVVGVPDDDLGQRIVAYVVGDV
CEEHHCCCHHHHHHCCCEEEECCCEEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHHCC
AEDDVIGFVAQQLSVHKRPREVRRVDALPRNAMGKVLKKELIT
CCHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]