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The map label for this gene is ispH [H]

Identifier: 169628350

GI number: 169628350

Start: 1264041

End: 1265054

Strand: Direct

Name: ispH [H]

Synonym: MAB_1257

Alternate gene names: 169628350

Gene position: 1264041-1265054 (Clockwise)

Preceding gene: 169628345

Following gene: 169628354

Centisome position: 24.95

GC content: 66.67

Gene sequence:

>1014_bases
ATGGCTGCAGTCGATATGGGCACTCCGGGGATGGTGAGCACCGCGATGCGCACAGTCGGCCCGGCGATCAGCAAGCGCGT
GCTGCTGGCTGAGCCGCGCGGCTACTGCGCGGGTGTGGACCGTGCCGTCGAAACGGTGGAGCGTGCCTTGGAGAAGCACG
GCGCCCCCGTTTACGTGCGTCACGAGATCGTGCACAACCGCTATGTGGTGGACACGCTGGCCAAGGCCGGCGCCATTTTC
GTCGACGAGACCGATGAGGTGCCCGAGGGCGCCATCGTGGTCTTCTCGGCCCACGGGGTGGCGCCGACGGTCCACGAGAA
GGCCGCCGAACGCAATCTGAAGACCATCGACGCCACCTGCCCGCTGGTCACCAAGGTGCACCAGGAAGCCAAGCGCTTCG
CGCGTGACGACTTCGACATTCTGCTCATCGGCCACGAAGGCCATGAGGAAGTCATCGGCACCGCCGGTGAGGCGCCCGAC
CACGTACAACTGGTCGACGGCCCCAACGCGGTGGACAACGTCACGGTGCGTGACGAGAACAAGGTCATTTGGCTGTCCCA
GACGACGCTGAGCGTCGACGAGACCATGGAGACGGTGCAGCGCCTGCGTGAGCGCTTTCCGACGCTGCAGGACCCGCCCA
GTGACGACATCTGCTACGCCACCCAGAACCGCCAGGTCGCGGTGAAGGCGATGGCCCCGGAATGCGAGTTGGTGATTGTC
GTCGGTTCGCGTAACTCGTCGAACTCGGTGCGGCTGGTTGAGGTCGCCCTGAATGCGGGGTCGCGGGCGTCCTACCTGGT
CGACTACGCCGAGGACATCGATCCGGCGTGGCTCGAGGGAGTGACCACGGTCGGCGTCACCTCGGGTGCTTCGGTGCCGG
AGGTGCTGGTGCGCGGTGTGCTGGAGCGGCTGGCCGAGCATGGATACGGCGAGGTGCAGCCGGTCACCACCGCCAACGAG
ACGTTGGTGTTCGCGCTGCCGCGTGAACTGCGGGCCTCGCGGCGCTCTGGCTAG

Upstream 100 bases:

>100_bases
CCCCTAGAAGCAACCGGACGCCGAACGGAGGACGAATCATAGGTTCAAGCTTGCCTTACGTCGGTCGTGCAGCGAAAGGC
TGGGCCGTAAGCTGGTAACT

Downstream 100 bases:

>100_bases
CGGTCGTAGTCCCAGGCGTCGTACCGGTCCTGGCGGCGCCGCGGCGGTACCGGCGGACGTTCGTCCCGGTACCTGACGGC
AGGCCGGTGCGGCTCGCGTG

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MAAVDMGTPGMVSTAMRTVGPAISKRVLLAEPRGYCAGVDRAVETVERALEKHGAPVYVRHEIVHNRYVVDTLAKAGAIF
VDETDEVPEGAIVVFSAHGVAPTVHEKAAERNLKTIDATCPLVTKVHQEAKRFARDDFDILLIGHEGHEEVIGTAGEAPD
HVQLVDGPNAVDNVTVRDENKVIWLSQTTLSVDETMETVQRLRERFPTLQDPPSDDICYATQNRQVAVKAMAPECELVIV
VGSRNSSNSVRLVEVALNAGSRASYLVDYAEDIDPAWLEGVTTVGVTSGASVPEVLVRGVLERLAEHGYGEVQPVTTANE
TLVFALPRELRASRRSG

Sequences:

>Translated_337_residues
MAAVDMGTPGMVSTAMRTVGPAISKRVLLAEPRGYCAGVDRAVETVERALEKHGAPVYVRHEIVHNRYVVDTLAKAGAIF
VDETDEVPEGAIVVFSAHGVAPTVHEKAAERNLKTIDATCPLVTKVHQEAKRFARDDFDILLIGHEGHEEVIGTAGEAPD
HVQLVDGPNAVDNVTVRDENKVIWLSQTTLSVDETMETVQRLRERFPTLQDPPSDDICYATQNRQVAVKAMAPECELVIV
VGSRNSSNSVRLVEVALNAGSRASYLVDYAEDIDPAWLEGVTTVGVTSGASVPEVLVRGVLERLAEHGYGEVQPVTTANE
TLVFALPRELRASRRSG
>Mature_336_residues
AAVDMGTPGMVSTAMRTVGPAISKRVLLAEPRGYCAGVDRAVETVERALEKHGAPVYVRHEIVHNRYVVDTLAKAGAIFV
DETDEVPEGAIVVFSAHGVAPTVHEKAAERNLKTIDATCPLVTKVHQEAKRFARDDFDILLIGHEGHEEVIGTAGEAPDH
VQLVDGPNAVDNVTVRDENKVIWLSQTTLSVDETMETVQRLRERFPTLQDPPSDDICYATQNRQVAVKAMAPECELVIVV
GSRNSSNSVRLVEVALNAGSRASYLVDYAEDIDPAWLEGVTTVGVTSGASVPEVLVRGVLERLAEHGYGEVQPVTTANET
LVFALPRELRASRRSG

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=313, Percent_Identity=52.7156549520767, Blast_Score=331, Evalue=4e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 36476; Mature: 36344

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAVDMGTPGMVSTAMRTVGPAISKRVLLAEPRGYCAGVDRAVETVERALEKHGAPVYVR
CCCCCCCCCCHHHHHHHHHCHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEE
HEIVHNRYVVDTLAKAGAIFVDETDEVPEGAIVVFSAHGVAPTVHEKAAERNLKTIDATC
EHHHHCHHHHHHHHHCCEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEECCC
PLVTKVHQEAKRFARDDFDILLIGHEGHEEVIGTAGEAPDHVQLVDGPNAVDNVTVRDEN
HHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHCCCCCCCCEEEEECCCCCCCCEEEECCC
KVIWLSQTTLSVDETMETVQRLRERFPTLQDPPSDDICYATQNRQVAVKAMAPECELVIV
EEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEEEECCCCEEEEE
VGSRNSSNSVRLVEVALNAGSRASYLVDYAEDIDPAWLEGVTTVGVTSGASVPEVLVRGV
ECCCCCCCCEEEEEEEECCCCCHHEEEEHHHCCCHHHHCCEEEEEECCCCCHHHHHHHHH
LERLAEHGYGEVQPVTTANETLVFALPRELRASRRSG
HHHHHHCCCCCEEEECCCCCEEEEECCHHHHHHCCCC
>Mature Secondary Structure 
AAVDMGTPGMVSTAMRTVGPAISKRVLLAEPRGYCAGVDRAVETVERALEKHGAPVYVR
CCCCCCCCCHHHHHHHHHCHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEE
HEIVHNRYVVDTLAKAGAIFVDETDEVPEGAIVVFSAHGVAPTVHEKAAERNLKTIDATC
EHHHHCHHHHHHHHHCCEEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEECCC
PLVTKVHQEAKRFARDDFDILLIGHEGHEEVIGTAGEAPDHVQLVDGPNAVDNVTVRDEN
HHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHCCCCCCCCEEEEECCCCCCCCEEEECCC
KVIWLSQTTLSVDETMETVQRLRERFPTLQDPPSDDICYATQNRQVAVKAMAPECELVIV
EEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEEEECCCCEEEEE
VGSRNSSNSVRLVEVALNAGSRASYLVDYAEDIDPAWLEGVTTVGVTSGASVPEVLVRGV
ECCCCCCCCEEEEEEEECCCCCHHEEEEHHHCCCHHHHCCEEEEEECCCCCHHHHHHHHH
LERLAEHGYGEVQPVTTANETLVFALPRELRASRRSG
HHHHHHCCCCCEEEECCCCCEEEEECCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA