Click here to switch to the map view.

The map label for this gene is metB [H]

Identifier: 169628290

GI number: 169628290

Start: 1211929

End: 1213101

Strand: Direct

Name: metB [H]

Synonym: MAB_1197

Alternate gene names: 169628290

Gene position: 1211929-1213101 (Clockwise)

Preceding gene: 169628289

Following gene: 169628292

Centisome position: 23.92

GC content: 65.47

Gene sequence:

>1173_bases
ATGAGTGAGCAGCGCAGCGCCGCCGACGCGTCTCGGTGGCAAGGGTTTTCCACAAGGGCCATCCACGGCGGTTTCCATGC
CGATCCGCAGACCGGTGCGGTCAACGTGCCGATCTACGCCAGTTCGACCTTCGCCCAGGACGGCGTCGGCCAGCTGCGTG
GAGGATTCGAATACGCACGCACCGGCAATCCCACGCGTGCTGTTCTCGAAGCCTCTTTGGCGGCATTGGAAGACGCCCAC
TTCGGCAGGGCGTTCAGTTCCGGGATGGCGGCCACCGACTGCGTGTTGCGCGCGCTGCTGAGGCCGGGCGATCACCTGAT
CATCCCCAATGATGCCTACGGCGGCACCTTCCGGCTGATCGACAAGGTCTTCTCGCAGTGGGGGATCAGACACACTCCGG
TGCCGGTGGCGGACGTCGATGCCATCCGCGCGGCAATTACCCCCACCACCAAGCTGATCTGGCTGGAGACGCCGACCAAC
CCGCTGCTGAGTATCGCCGATATTGCGGCGGTCGCCCAGGTGGCGGCGGAGCATTCCGTGAAGCTGTTGGTGGACAACAC
TTTCGCCTCACCGGCGCTACAGCAGCCGCTGAATCTCGGCGCCGATATCGCGCTGCACTCCACCACCAAATACATTGGCG
GACATTCCGATGTCGTTGGGGGAGCCTTGCTCACCAACGACGAGGAGCTGGACACCGCCTTCGCGTTCCTGCAGAACGGG
GCGGGTGCGGTGCCGGGCCCGTTCGACGGCTACCTGACCTACCGGGGTATCAAGACCTTGGCGTTGCGCATGCAGCGACA
CAGCGAGAACGCGCAAGCGGTGGCCGAGTACCTGGCCGGCCACTCCGCCGTCGCGCAGACGATCTACCCGGGGCTGGAAA
GCCACCCGGGGCATGCCGTGGCCGCCAGGCAGATGCGTGGCTTCGGCGGCATGGTCAGCGTGCGGTTGGCCGGAGGACGC
CAGGCGGCACTCGACTTGTGCTCGCGAACAGAGCTTTTCATTCTGGCTGAGTCCTTGGGCGGGGTGGAGTCCTTGATCGA
GCACCCGGGAGCCATGACGCACGCGTCGACTGCGGGATCGCTGCTGGAGGTGCCGGACGATCTGGTGCGGTTGTCGGTGG
GCATCGAGGAGGTCGGCGATCTGATCGGCGACCTCGAGCAGGCGTTGCGTTAA

Upstream 100 bases:

>100_bases
CGACATGCTAGTGAAGACCGTCGTCGTTCCCAAGTAGAAGTAATCGTCAGGCGGCGCTGGGCTGTCTCACTCCTATCGGT
CCGGTATAGGCTCGGACCCG

Downstream 100 bases:

>100_bases
GACATCGAATGTAGGGCCGCCGAGATCACCGCGGCGGTCACCGCGAGATTGACTGCCGGGCCCCGGTTGTCGCGGGTCTG
GGCCGCCCACCCGCCGTCGG

Product: cystathionine gamma-synthase (CGS)

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MSEQRSAADASRWQGFSTRAIHGGFHADPQTGAVNVPIYASSTFAQDGVGQLRGGFEYARTGNPTRAVLEASLAALEDAH
FGRAFSSGMAATDCVLRALLRPGDHLIIPNDAYGGTFRLIDKVFSQWGIRHTPVPVADVDAIRAAITPTTKLIWLETPTN
PLLSIADIAAVAQVAAEHSVKLLVDNTFASPALQQPLNLGADIALHSTTKYIGGHSDVVGGALLTNDEELDTAFAFLQNG
AGAVPGPFDGYLTYRGIKTLALRMQRHSENAQAVAEYLAGHSAVAQTIYPGLESHPGHAVAARQMRGFGGMVSVRLAGGR
QAALDLCSRTELFILAESLGGVESLIEHPGAMTHASTAGSLLEVPDDLVRLSVGIEEVGDLIGDLEQALR

Sequences:

>Translated_390_residues
MSEQRSAADASRWQGFSTRAIHGGFHADPQTGAVNVPIYASSTFAQDGVGQLRGGFEYARTGNPTRAVLEASLAALEDAH
FGRAFSSGMAATDCVLRALLRPGDHLIIPNDAYGGTFRLIDKVFSQWGIRHTPVPVADVDAIRAAITPTTKLIWLETPTN
PLLSIADIAAVAQVAAEHSVKLLVDNTFASPALQQPLNLGADIALHSTTKYIGGHSDVVGGALLTNDEELDTAFAFLQNG
AGAVPGPFDGYLTYRGIKTLALRMQRHSENAQAVAEYLAGHSAVAQTIYPGLESHPGHAVAARQMRGFGGMVSVRLAGGR
QAALDLCSRTELFILAESLGGVESLIEHPGAMTHASTAGSLLEVPDDLVRLSVGIEEVGDLIGDLEQALR
>Mature_389_residues
SEQRSAADASRWQGFSTRAIHGGFHADPQTGAVNVPIYASSTFAQDGVGQLRGGFEYARTGNPTRAVLEASLAALEDAHF
GRAFSSGMAATDCVLRALLRPGDHLIIPNDAYGGTFRLIDKVFSQWGIRHTPVPVADVDAIRAAITPTTKLIWLETPTNP
LLSIADIAAVAQVAAEHSVKLLVDNTFASPALQQPLNLGADIALHSTTKYIGGHSDVVGGALLTNDEELDTAFAFLQNGA
GAVPGPFDGYLTYRGIKTLALRMQRHSENAQAVAEYLAGHSAVAQTIYPGLESHPGHAVAARQMRGFGGMVSVRLAGGRQ
AALDLCSRTELFILAESLGGVESLIEHPGAMTHASTAGSLLEVPDDLVRLSVGIEEVGDLIGDLEQALR

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=385, Percent_Identity=48.0519480519481, Blast_Score=327, Evalue=1e-89,
Organism=Homo sapiens, GI299473759, Length=385, Percent_Identity=43.8961038961039, Blast_Score=284, Evalue=1e-76,
Organism=Homo sapiens, GI25453487, Length=384, Percent_Identity=42.7083333333333, Blast_Score=261, Evalue=1e-69,
Organism=Escherichia coli, GI1790375, Length=377, Percent_Identity=43.5013262599469, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1789383, Length=393, Percent_Identity=30.0254452926209, Blast_Score=155, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17538055, Length=384, Percent_Identity=47.65625, Blast_Score=332, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI17560056, Length=384, Percent_Identity=46.6145833333333, Blast_Score=330, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI71980708, Length=379, Percent_Identity=40.1055408970976, Blast_Score=262, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6319307, Length=385, Percent_Identity=47.2727272727273, Blast_Score=326, Evalue=4e-90,
Organism=Saccharomyces cerevisiae, GI6321254, Length=403, Percent_Identity=32.258064516129, Blast_Score=178, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6321170, Length=310, Percent_Identity=30.6451612903226, Blast_Score=149, Evalue=7e-37,
Organism=Saccharomyces cerevisiae, GI6323333, Length=437, Percent_Identity=29.2906178489703, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6321904, Length=391, Percent_Identity=29.6675191815857, Blast_Score=141, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19922554, Length=382, Percent_Identity=47.6439790575916, Blast_Score=347, Evalue=8e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 41090; Mature: 40959

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQRSAADASRWQGFSTRAIHGGFHADPQTGAVNVPIYASSTFAQDGVGQLRGGFEYAR
CCCCHHHHHHHHCCCCCCEEECCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHCCCHHHH
TGNPTRAVLEASLAALEDAHFGRAFSSGMAATDCVLRALLRPGDHLIIPNDAYGGTFRLI
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHH
DKVFSQWGIRHTPVPVADVDAIRAAITPTTKLIWLETPTNPLLSIADIAAVAQVAAEHSV
HHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCE
KLLVDNTFASPALQQPLNLGADIALHSTTKYIGGHSDVVGGALLTNDEELDTAFAFLQNG
EEEEECCCCCHHHHHHHCCCCCEEEECCHHHCCCCCHHCCCEEECCCHHHHHHHHHHHCC
AGAVPGPFDGYLTYRGIKTLALRMQRHSENAQAVAEYLAGHSAVAQTIYPGLESHPGHAV
CCCCCCCCCCEEHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHCCCCCCCCCHHH
AARQMRGFGGMVSVRLAGGRQAALDLCSRTELFILAESLGGVESLIEHPGAMTHASTAGS
HHHHHHCCCCEEEEEECCCCHHHHHHHCCCCEEEEEHHHCCHHHHHHCCCCCCCCCCCCC
LLEVPDDLVRLSVGIEEVGDLIGDLEQALR
HHHCCHHHHHHHCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEQRSAADASRWQGFSTRAIHGGFHADPQTGAVNVPIYASSTFAQDGVGQLRGGFEYAR
CCCHHHHHHHHCCCCCCEEECCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHCCCHHHH
TGNPTRAVLEASLAALEDAHFGRAFSSGMAATDCVLRALLRPGDHLIIPNDAYGGTFRLI
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHH
DKVFSQWGIRHTPVPVADVDAIRAAITPTTKLIWLETPTNPLLSIADIAAVAQVAAEHSV
HHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCE
KLLVDNTFASPALQQPLNLGADIALHSTTKYIGGHSDVVGGALLTNDEELDTAFAFLQNG
EEEEECCCCCHHHHHHHCCCCCEEEECCHHHCCCCCHHCCCEEECCCHHHHHHHHHHHCC
AGAVPGPFDGYLTYRGIKTLALRMQRHSENAQAVAEYLAGHSAVAQTIYPGLESHPGHAV
CCCCCCCCCCEEHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHCCCCCCCCCHHH
AARQMRGFGGMVSVRLAGGRQAALDLCSRTELFILAESLGGVESLIEHPGAMTHASTAGS
HHHHHHCCCCEEEEEECCCCHHHHHHHCCCCEEEEEHHHCCHHHHHHCCCCCCCCCCCCC
LLEVPDDLVRLSVGIEEVGDLIGDLEQALR
HHHCCHHHHHHHCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]