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The map label for this gene is caiD [H]

Identifier: 169628164

GI number: 169628164

Start: 1080609

End: 1081388

Strand: Reverse

Name: caiD [H]

Synonym: MAB_1069c

Alternate gene names: 169628164

Gene position: 1081388-1080609 (Counterclockwise)

Preceding gene: 169628165

Following gene: 169628163

Centisome position: 21.34

GC content: 68.21

Gene sequence:

>780_bases
ATGAGTGACACCCTGGTCCAGTACGAGGTCGAAGGCCGTGCCGCACGCCTGACGCTCGACTCGCCGCACAACCGTAATGC
GTTGTCCAGCGGGCTGGTACGGCAATTGCGGGAGGGACTGCAGCGGGCCGCCGCGGACCAGACGGTGCGCGCCGTGGTAC
TCACCCACACCGGCAACACCTTCTGTGCCGGAGCGGATCTGTCTGAGGCCTCTGAGGGGTCACCGCAGGACATCGCCAAG
GACCGGGCGGGTCAGCTCACCGCACTGCTGCGCGACATCCTGGAGCTGCCACTGCCCGTCATCATCGCCGTCAATGGCCA
TGTGCGGGCCGGCGGTTTCGGTCTCGTCGGTGCGGCCGATATCGCGGTGGCGGGTCCGGACAGCACGTTCGCGCTCACCG
AGGCACGCATCGGGGTGGCGCCATCGATCATCTCGCTGACCCTGCTGCCCCGGCTCACCAGCCGGGCCGCGGGACGCTAC
TTCGTCACCGGCGAGAAGTTCGACGCGCGGGTGGCCGCGGAGATCGGTTTGGTCACCCAGTCCGTCGACAGCGCCGAGGC
TGTCGAGGCCGCGGTCACCTCGTTGGTGGACGACATCGCGCTGGGTTCACCACAGGGACTGGCCGAGTCGAAGCGGCTCA
CCACTGCGCCGATCCTCGCCGATTTCGACGCGCGTGCAGTCGAGTTGACGGAAACCTCGGCGCGCCTGTTCGTGTCGCCC
GAGGCACAGGAGGGCATGCTGGCGTTCCTCCAGAAGCGGCCTCCCGCCTGGCAAGTGTGA

Upstream 100 bases:

>100_bases
GTATCGCCCCGGTCAGCCGCGAGATGATTCTCAACTTCGTCGCGCAGACCTCCCTTGGCCTGCCCCGTTCGTACTGATTC
GTACTCAAGGAGACGCCCCG

Downstream 100 bases:

>100_bases
ACCACGCGCCACTACGTATGTAGTGCGCTGAGCAACACTATTGAACCGCGTGCCTTTAAGCGCCGTTCGTCGTAGGCTCG
GCGGTGATGAGCAACCTCCC

Product: enoyl-CoA hydratase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MSDTLVQYEVEGRAARLTLDSPHNRNALSSGLVRQLREGLQRAAADQTVRAVVLTHTGNTFCAGADLSEASEGSPQDIAK
DRAGQLTALLRDILELPLPVIIAVNGHVRAGGFGLVGAADIAVAGPDSTFALTEARIGVAPSIISLTLLPRLTSRAAGRY
FVTGEKFDARVAAEIGLVTQSVDSAEAVEAAVTSLVDDIALGSPQGLAESKRLTTAPILADFDARAVELTETSARLFVSP
EAQEGMLAFLQKRPPAWQV

Sequences:

>Translated_259_residues
MSDTLVQYEVEGRAARLTLDSPHNRNALSSGLVRQLREGLQRAAADQTVRAVVLTHTGNTFCAGADLSEASEGSPQDIAK
DRAGQLTALLRDILELPLPVIIAVNGHVRAGGFGLVGAADIAVAGPDSTFALTEARIGVAPSIISLTLLPRLTSRAAGRY
FVTGEKFDARVAAEIGLVTQSVDSAEAVEAAVTSLVDDIALGSPQGLAESKRLTTAPILADFDARAVELTETSARLFVSP
EAQEGMLAFLQKRPPAWQV
>Mature_258_residues
SDTLVQYEVEGRAARLTLDSPHNRNALSSGLVRQLREGLQRAAADQTVRAVVLTHTGNTFCAGADLSEASEGSPQDIAKD
RAGQLTALLRDILELPLPVIIAVNGHVRAGGFGLVGAADIAVAGPDSTFALTEARIGVAPSIISLTLLPRLTSRAAGRYF
VTGEKFDARVAAEIGLVTQSVDSAEAVEAAVTSLVDDIALGSPQGLAESKRLTTAPILADFDARAVELTETSARLFVSPE
AQEGMLAFLQKRPPAWQV

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=255, Percent_Identity=28.2352941176471, Blast_Score=91, Evalue=9e-19,
Organism=Homo sapiens, GI194097323, Length=242, Percent_Identity=31.404958677686, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI4502327, Length=254, Percent_Identity=25.9842519685039, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI70995211, Length=209, Percent_Identity=29.1866028708134, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI68989263, Length=204, Percent_Identity=30.8823529411765, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI221136756, Length=241, Percent_Identity=24.4813278008299, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI221136753, Length=241, Percent_Identity=24.4813278008299, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI221307494, Length=241, Percent_Identity=24.4813278008299, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI37594469, Length=203, Percent_Identity=29.5566502463054, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI37594471, Length=203, Percent_Identity=29.5566502463054, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI260274832, Length=182, Percent_Identity=26.9230769230769, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI25453481, Length=233, Percent_Identity=21.0300429184549, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI51538573, Length=233, Percent_Identity=21.0300429184549, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI45643119, Length=182, Percent_Identity=26.9230769230769, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI260275230, Length=182, Percent_Identity=26.9230769230769, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1787659, Length=259, Percent_Identity=31.2741312741313, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI221142681, Length=264, Percent_Identity=31.0606060606061, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1788597, Length=248, Percent_Identity=27.8225806451613, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787660, Length=252, Percent_Identity=30.952380952381, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI87082183, Length=266, Percent_Identity=25.5639097744361, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790281, Length=192, Percent_Identity=28.6458333333333, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25145438, Length=244, Percent_Identity=31.1475409836066, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=256, Percent_Identity=30.078125, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17560910, Length=256, Percent_Identity=26.953125, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=215, Percent_Identity=27.4418604651163, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17540306, Length=241, Percent_Identity=26.9709543568465, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17549921, Length=216, Percent_Identity=31.9444444444444, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17540714, Length=170, Percent_Identity=31.1764705882353, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17536985, Length=216, Percent_Identity=25.462962962963, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17535521, Length=251, Percent_Identity=23.9043824701195, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24653139, Length=248, Percent_Identity=30.241935483871, Blast_Score=97, Evalue=7e-21,
Organism=Drosophila melanogaster, GI19922422, Length=262, Percent_Identity=28.6259541984733, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI20129971, Length=259, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24653477, Length=259, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI19920382, Length=209, Percent_Identity=26.7942583732057, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 27165; Mature: 27034

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTLVQYEVEGRAARLTLDSPHNRNALSSGLVRQLREGLQRAAADQTVRAVVLTHTGNT
CCCCEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCE
FCAGADLSEASEGSPQDIAKDRAGQLTALLRDILELPLPVIIAVNGHVRAGGFGLVGAAD
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEECCCEEEECCE
IAVAGPDSTFALTEARIGVAPSIISLTLLPRLTSRAAGRYFVTGEKFDARVAAEIGLVTQ
EEEECCCCCEEEEHHHCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHH
SVDSAEAVEAAVTSLVDDIALGSPQGLAESKRLTTAPILADFDARAVELTETSARLFVSP
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCEEECCCCCEEEEECCCEEEEECC
EAQEGMLAFLQKRPPAWQV
CCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SDTLVQYEVEGRAARLTLDSPHNRNALSSGLVRQLREGLQRAAADQTVRAVVLTHTGNT
CCCEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCE
FCAGADLSEASEGSPQDIAKDRAGQLTALLRDILELPLPVIIAVNGHVRAGGFGLVGAAD
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCEEEECCCEEEECCE
IAVAGPDSTFALTEARIGVAPSIISLTLLPRLTSRAAGRYFVTGEKFDARVAAEIGLVTQ
EEEECCCCCEEEEHHHCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHH
SVDSAEAVEAAVTSLVDDIALGSPQGLAESKRLTTAPILADFDARAVELTETSARLFVSP
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCEEECCCCCEEEEECCCEEEEECC
EAQEGMLAFLQKRPPAWQV
CCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA