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The map label for this gene is ynfL [H]

Identifier: 169628089

GI number: 169628089

Start: 1000098

End: 1000991

Strand: Reverse

Name: ynfL [H]

Synonym: MAB_0993c

Alternate gene names: 169628089

Gene position: 1000991-1000098 (Counterclockwise)

Preceding gene: 169628092

Following gene: 169628087

Centisome position: 19.75

GC content: 66.0

Gene sequence:

>894_bases
ATGGCAGAGACCGCCCCGCTGGACCTGGACCTACGCCTGGTCCGGTACTTCACCGTGGTGGCCGAGCAGCGACATTTCGG
CCGGGCCAGCACCGAGCTGCGGGTGGCGCAACCCTCGTTGAGCCGCCAAATCAGCAGACTGGAACAGCAGCTCGGTGTGC
GGCTGCTCGACCGCACACCGCAAGGCACCACACTCACGGAAGCAGGCGAGGTCTTCCTCCCCAAGGCAAAGACCCTGTTG
CGGTCGGCCGATCAGGCCGCAGCGCATGCGCGTGCCGCGGCGCAACCCAGCCACCTCACGGTCGGCTACTCGATGGGGAT
CATCGTGACGCCCGCCGTATTACAGATGCGTCAGAAGCATCCAGACGCCGATATCCGCGCCACCCACCTGAAATGGGACG
AAGCGCGGGCCGCGCTGCTTGATCACCGGGTGGACGCGGTGGTGGCACGCCTACCTTTCTCCACCGACCAGTTGCACGTC
ACGATCCTGTACGACGAACCGCGCGTCGTGCTGCTGCCATCCGATCACCCTCTGGCTCAACATGACTCGCTGACCCTAGC
CGACATCGCGAACGAACCGATGCCACGGATACGCAATGCCGACCCGTCATGGAGTGCCTACTGGCGCGTGGAGCCGCGAC
CCGATGGCAGCCTGGCACCTGACGGGCCCGTCATCGATGCCATCGAGGACAAATTCGAAGTAATTGCGTCAGGCCAGGCC
ATCTCCCTATCGGCGGGCGCGCACGGCAGCACCATGCGGCCCGATATCGTGGCCGTCCCACTTGAGGGGGTGGAACCTAG
CCACGTGGCCATAGCCACCCGCGCCGACGACCGGGGACGACTCGTCGCAGCGTTCCGCAAGGTCGCGCAAGCCGTCCTGA
CCGGACCGGGCTAG

Upstream 100 bases:

>100_bases
CCAAGACCGATTACTCATCGGGCAATACCAATCAGGCATCACTACATTTCAGAGTGCCTATTTCTCACATTGCCGCTCTT
CATACGCATACCATGTGGGT

Downstream 100 bases:

>100_bases
AGGATCTCGTCGACCGCCTCCGTGGGCCGGGCCATCCGGGTCCCCTTGGCGGTCACCACGAACGGACGCTGAATTAACCG
CGGGTGCGCAACCATTGCGT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MAETAPLDLDLRLVRYFTVVAEQRHFGRASTELRVAQPSLSRQISRLEQQLGVRLLDRTPQGTTLTEAGEVFLPKAKTLL
RSADQAAAHARAAAQPSHLTVGYSMGIIVTPAVLQMRQKHPDADIRATHLKWDEARAALLDHRVDAVVARLPFSTDQLHV
TILYDEPRVVLLPSDHPLAQHDSLTLADIANEPMPRIRNADPSWSAYWRVEPRPDGSLAPDGPVIDAIEDKFEVIASGQA
ISLSAGAHGSTMRPDIVAVPLEGVEPSHVAIATRADDRGRLVAAFRKVAQAVLTGPG

Sequences:

>Translated_297_residues
MAETAPLDLDLRLVRYFTVVAEQRHFGRASTELRVAQPSLSRQISRLEQQLGVRLLDRTPQGTTLTEAGEVFLPKAKTLL
RSADQAAAHARAAAQPSHLTVGYSMGIIVTPAVLQMRQKHPDADIRATHLKWDEARAALLDHRVDAVVARLPFSTDQLHV
TILYDEPRVVLLPSDHPLAQHDSLTLADIANEPMPRIRNADPSWSAYWRVEPRPDGSLAPDGPVIDAIEDKFEVIASGQA
ISLSAGAHGSTMRPDIVAVPLEGVEPSHVAIATRADDRGRLVAAFRKVAQAVLTGPG
>Mature_296_residues
AETAPLDLDLRLVRYFTVVAEQRHFGRASTELRVAQPSLSRQISRLEQQLGVRLLDRTPQGTTLTEAGEVFLPKAKTLLR
SADQAAAHARAAAQPSHLTVGYSMGIIVTPAVLQMRQKHPDADIRATHLKWDEARAALLDHRVDAVVARLPFSTDQLHVT
ILYDEPRVVLLPSDHPLAQHDSLTLADIANEPMPRIRNADPSWSAYWRVEPRPDGSLAPDGPVIDAIEDKFEVIASGQAI
SLSAGAHGSTMRPDIVAVPLEGVEPSHVAIATRADDRGRLVAAFRKVAQAVLTGPG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=194, Percent_Identity=35.5670103092784, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1788887, Length=277, Percent_Identity=31.7689530685921, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1790399, Length=191, Percent_Identity=29.8429319371728, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI2367136, Length=279, Percent_Identity=27.2401433691756, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI145693105, Length=221, Percent_Identity=24.4343891402715, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI157672245, Length=192, Percent_Identity=30.7291666666667, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787589, Length=151, Percent_Identity=35.0993377483444, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87081904, Length=193, Percent_Identity=29.0155440414508, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788297, Length=146, Percent_Identity=28.7671232876712, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI48994962, Length=99, Percent_Identity=35.3535353535354, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32303; Mature: 32172

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETAPLDLDLRLVRYFTVVAEQRHFGRASTELRVAQPSLSRQISRLEQQLGVRLLDRTP
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHCCEEECCCC
QGTTLTEAGEVFLPKAKTLLRSADQAAAHARAAAQPSHLTVGYSMGIIVTPAVLQMRQKH
CCCEECCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEECHHHHHHHHHC
PDADIRATHLKWDEARAALLDHRVDAVVARLPFSTDQLHVTILYDEPRVVLLPSDHPLAQ
CCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEECCCCCCCC
HDSLTLADIANEPMPRIRNADPSWSAYWRVEPRPDGSLAPDGPVIDAIEDKFEVIASGQA
CCCEEHHHHCCCCCCHHCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCE
ISLSAGAHGSTMRPDIVAVPLEGVEPSHVAIATRADDRGRLVAAFRKVAQAVLTGPG
EEEECCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AETAPLDLDLRLVRYFTVVAEQRHFGRASTELRVAQPSLSRQISRLEQQLGVRLLDRTP
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHCCEEECCCC
QGTTLTEAGEVFLPKAKTLLRSADQAAAHARAAAQPSHLTVGYSMGIIVTPAVLQMRQKH
CCCEECCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEECHHHHHHHHHC
PDADIRATHLKWDEARAALLDHRVDAVVARLPFSTDQLHVTILYDEPRVVLLPSDHPLAQ
CCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEECCCCCCCC
HDSLTLADIANEPMPRIRNADPSWSAYWRVEPRPDGSLAPDGPVIDAIEDKFEVIASGQA
CCCEEHHHHCCCCCCHHCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCE
ISLSAGAHGSTMRPDIVAVPLEGVEPSHVAIATRADDRGRLVAAFRKVAQAVLTGPG
EEEECCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]