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The map label for this gene is czcO [H]

Identifier: 169628055

GI number: 169628055

Start: 965554

End: 966642

Strand: Reverse

Name: czcO [H]

Synonym: MAB_0958c

Alternate gene names: 169628055

Gene position: 966642-965554 (Counterclockwise)

Preceding gene: 169628058

Following gene: 169628053

Centisome position: 19.08

GC content: 69.61

Gene sequence:

>1089_bases
ATGACTACCGTCGCCGTGATTGGTGGCGGGCAGGCCGGGATCGCGGCGGGATACTTCCTACGCCGGCGCGGTCTGGAGCC
GCTGAGCCGGCGACAGCCCGCCGATGCGCCCGGCCACCGGTTCGTCATCCTGGATCACTCGCCCGGCCCGGGCGGGGCCT
GGCAGTTCCGCTGGCCGACGCTGACGCTGGATAACACCAACCGCATCTACCAGCTGCCCGGGCTGCCGTTCGACGAGATG
GGGACCATCCGGGCCTCCACCGCCATGCCCAGGTACTTCGGCGAATACGAGGACCGGTATGCCCTCGATGTGCGCCGGCC
GGTTCACGTGCGCGCGGTGCGGCCGGCCCGGAGAGGCTTCACGATCGACACCTCGGTGGGCGAATTTCCCGTCGACGGGC
TGATCAATGCCACGGGGACGTGGGACAAACCGTTCGTCCCGTTCGTGCCCGGCGCCGGCACCTTCCGGGGACGTCAGCTG
CACACCCACGACTACCAGCGACCGGAGGAGTTCACCGGCCAGCATGTGCTCGTGGTGGGCGGCGGTATCTCGGCCGTGCA
GCTGCTCATGGAAATCTCCGGGATCGCCTCCGTCACCACCTCGTGGGTGACCCGGCGCGAGCCGGTGTTCGTCACCGACC
TCACCGTCGAGCGGCTGCGCGCGGCGGTGGCGGGTGTCGATGAGCGGTCGCGTCGGGGCGAACCGCAGCGGTCGGTGGTC
TCGGCGACGGGGCTGCCGTGGACCGCGGAAACCGCCGAAGCCTCGCGGCGTGGGGTCCTGACCCGACGCCCCATGTTCAG
CCGGATCACCCCGACCGGGGTCACCTGGCCCGACGGCAGCTCCCTGGAAGTCGACGTGATCCTGTGGTGCACCGGATTTC
GGCACGCGCTGGACCACCTGGCACCCCTGCACCTACGCAACGGACGCGGCGGCATCACCATGACCGGCCGGCTGCTCACT
CAGGTCGCGGGGCAGCCCGCCATCCATCTGCTCGGCTATGGCTCGTCGGCAAGCACCATCGGAGCCAACCGCGCATCCCG
TGCCGCCGTGGTCGAGCTGATGAACTATCTAGAAGGACGTACTGCCTGA

Upstream 100 bases:

>100_bases
AGGTCACCGCGTTACGCCGCTCCGGGCGCTGCAGCTCAATGGTGGTGACGGGACCGTCGCTAGTTACACCGATCATGGCA
ACCACCCTAATATGGCGCCC

Downstream 100 bases:

>100_bases
GGGCTGCAGGACAAAACCGTGCTTCTGATCGCCCGTGACCACCTGGCAGGCGGTGACGCTACCCGCCCCGACGGCACACG
TGACGTTGCCATAGGTGAGC

Product: putative oxidoreductase

Products: NADP+; reduced thioredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MTTVAVIGGGQAGIAAGYFLRRRGLEPLSRRQPADAPGHRFVILDHSPGPGGAWQFRWPTLTLDNTNRIYQLPGLPFDEM
GTIRASTAMPRYFGEYEDRYALDVRRPVHVRAVRPARRGFTIDTSVGEFPVDGLINATGTWDKPFVPFVPGAGTFRGRQL
HTHDYQRPEEFTGQHVLVVGGGISAVQLLMEISGIASVTTSWVTRREPVFVTDLTVERLRAAVAGVDERSRRGEPQRSVV
SATGLPWTAETAEASRRGVLTRRPMFSRITPTGVTWPDGSSLEVDVILWCTGFRHALDHLAPLHLRNGRGGITMTGRLLT
QVAGQPAIHLLGYGSSASTIGANRASRAAVVELMNYLEGRTA

Sequences:

>Translated_362_residues
MTTVAVIGGGQAGIAAGYFLRRRGLEPLSRRQPADAPGHRFVILDHSPGPGGAWQFRWPTLTLDNTNRIYQLPGLPFDEM
GTIRASTAMPRYFGEYEDRYALDVRRPVHVRAVRPARRGFTIDTSVGEFPVDGLINATGTWDKPFVPFVPGAGTFRGRQL
HTHDYQRPEEFTGQHVLVVGGGISAVQLLMEISGIASVTTSWVTRREPVFVTDLTVERLRAAVAGVDERSRRGEPQRSVV
SATGLPWTAETAEASRRGVLTRRPMFSRITPTGVTWPDGSSLEVDVILWCTGFRHALDHLAPLHLRNGRGGITMTGRLLT
QVAGQPAIHLLGYGSSASTIGANRASRAAVVELMNYLEGRTA
>Mature_361_residues
TTVAVIGGGQAGIAAGYFLRRRGLEPLSRRQPADAPGHRFVILDHSPGPGGAWQFRWPTLTLDNTNRIYQLPGLPFDEMG
TIRASTAMPRYFGEYEDRYALDVRRPVHVRAVRPARRGFTIDTSVGEFPVDGLINATGTWDKPFVPFVPGAGTFRGRQLH
THDYQRPEEFTGQHVLVVGGGISAVQLLMEISGIASVTTSWVTRREPVFVTDLTVERLRAAVAGVDERSRRGEPQRSVVS
ATGLPWTAETAEASRRGVLTRRPMFSRITPTGVTWPDGSSLEVDVILWCTGFRHALDHLAPLHLRNGRGGITMTGRLLTQ
VAGQPAIHLLGYGSSASTIGANRASRAAVVELMNYLEGRTA

Specific function: Involved in potassium and divalent cation transport. Enhances the transport activity of the cation/potassium transporter CzcD [H]

COG id: COG2072

COG function: function code P; Predicted flavoprotein involved in K+ transport

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 808 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR000103 [H]

Pfam domain/function: PF07992 Pyr_redox_2 [H]

EC number: 1.8.1.9 [C]

Molecular weight: Translated: 39543; Mature: 39411

Theoretical pI: Translated: 10.61; Mature: 10.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVAVIGGGQAGIAAGYFLRRRGLEPLSRRQPADAPGHRFVILDHSPGPGGAWQFRWPT
CCEEEEEECCCHHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEEECCCCCCCEEEEECCE
LTLDNTNRIYQLPGLPFDEMGTIRASTAMPRYFGEYEDRYALDVRRPVHVRAVRPARRGF
EEECCCCCEEECCCCCHHHCCCEEHHHCCCHHHCCCCCCEEEECCCCEEEEEECHHHCCE
TIDTSVGEFPVDGLINATGTWDKPFVPFVPGAGTFRGRQLHTHDYQRPEEFTGQHVLVVG
EEECCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCHHHCCCEEEEEE
GGISAVQLLMEISGIASVTTSWVTRREPVFVTDLTVERLRAAVAGVDERSRRGEPQRSVV
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCHHHHCCCCHHHHE
SATGLPWTAETAEASRRGVLTRRPMFSRITPTGVTWPDGSSLEVDVILWCTGFRHALDHL
EECCCCCCCHHHHHHHCCCEECCCHHHCCCCCCCCCCCCCCEEEEEEEEECCHHHHHHHC
APLHLRNGRGGITMTGRLLTQVAGQPAIHLLGYGSSASTIGANRASRAAVVELMNYLEGR
CCEEEECCCCCEEEHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCC
TA
CC
>Mature Secondary Structure 
TTVAVIGGGQAGIAAGYFLRRRGLEPLSRRQPADAPGHRFVILDHSPGPGGAWQFRWPT
CEEEEEECCCHHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEEECCCCCCCEEEEECCE
LTLDNTNRIYQLPGLPFDEMGTIRASTAMPRYFGEYEDRYALDVRRPVHVRAVRPARRGF
EEECCCCCEEECCCCCHHHCCCEEHHHCCCHHHCCCCCCEEEECCCCEEEEEECHHHCCE
TIDTSVGEFPVDGLINATGTWDKPFVPFVPGAGTFRGRQLHTHDYQRPEEFTGQHVLVVG
EEECCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCHHHCCCEEEEEE
GGISAVQLLMEISGIASVTTSWVTRREPVFVTDLTVERLRAAVAGVDERSRRGEPQRSVV
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCHHHHCCCCHHHHE
SATGLPWTAETAEASRRGVLTRRPMFSRITPTGVTWPDGSSLEVDVILWCTGFRHALDHL
EECCCCCCCHHHHHHHCCCEECCCHHHCCCCCCCCCCCCCCEEEEEEEEECCHHHHHHHC
APLHLRNGRGGITMTGRLLTQVAGQPAIHLLGYGSSASTIGANRASRAAVVELMNYLEGR
CCEEEECCCCCEEEHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCC
TA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: NA

Kcat value (1/min): 1365 [C]

Specific activity: NA

Km value (mM): 0.008 {NADPH}} 0.125 {thioredoxin} 0.002 {thioredoxin}} 0.0017 {thioredoxin}} [C]

Substrates: NADPH; oxidized thioredoxin [C]

Specific reaction: NADPH + oxidized thioredoxin = NADP+ + reduced thioredoxin [C]

General reaction: Redox reaction [C]

Inhibitor: Ca2+; N-Ethyl maleimide; NADP+ [C]

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA