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The map label for this gene is thcD [H]

Identifier: 169628012

GI number: 169628012

Start: 905473

End: 906744

Strand: Reverse

Name: thcD [H]

Synonym: MAB_0913c

Alternate gene names: 169628012

Gene position: 906744-905473 (Counterclockwise)

Preceding gene: 169628013

Following gene: 169628000

Centisome position: 17.89

GC content: 63.36

Gene sequence:

>1272_bases
ATGCCAGCCCTCTCCGATTCGATGCCGGTGCCACCAGACCGTGAGGATGCCGCGGTGGTGATCGTCGGCGCCGGCCACGC
GGGTGTACAAGTCGCTGTCTCATTGCGCGAGTTCGGCTTTAGCGATCGAATCGTCATCGTGGACGCAGAAGCCAGATCGC
CGTATCAACGTCCGCCGCTGTCCAAGGAGTACCTGTGCGGCACCGTCGATCAGCCCCCACCCTTGCGGGCCCACGAATTC
TACGAAATACAGCGCATCGAATTTCGGCGTGCCGCCGCCGTCAGCATCGACCGCGCTCAACGCACAATCACGCTCGACGA
TGGTAGCCGCCTCGGCTATGGGCATCTGGTACTCGCCACCGGCGCCCGCAACCGGGAAATTCCGGTCCCCGGCGCCAACA
CGCCTGGCGTGCTTGGGCTGCGCACGCTCGATGACGCGCGCGATATTCGCGAGAGGATCGGCACCTCGGACGATATCGTG
ATCATCGGCGCAGGCTTTATCGGCCTCGAAGTTGCTGCCGCGGCCCGTAAGCGCGGCAAACGGGTGCATGTTGTCGAAGC
CGTCGACAGAGTGATGGCACGTGTTGTTTCGCCGCACGTGTCGCAGCACCTGGCGGATTTGCATGCACGTGAAGGCGTCA
ACGTGCATCTGGGACGCTCGTTGGCCGCCATCCGCACCGACCGCGCCGGAAAACTAGACACAGTCGAATTGACGTGTGGC
ACAACGATGGCCGCCGATCTGGTGGTCGTCGGCGTCGGTGTGGTACCCAACATTGAACTCGCGGCCGAGGCAGGGCTGGC
CGTAGACAACGGAGTTTGTGTGGATGCCGCGCTGCTCACCAAGGATCCTGCGATATCAGCGGTCGGCGATTGCGCGGCCT
TCCCCGTGTCTGGCTCGGGCGCCTCGATACGACTGGAATCGGTACAGAACGCGGTGGACCATGGTCGGTTCGTCGCTGCC
CGACTCACCGGATCCGTCGAAAACTACGTCGCCGTACCGTGGTTCTGGACGCACCAGTACTCCGCAAAACTCCAGATCGC
CGGAAAGGCCCTTGAGGTAAGCGAATCCGTGGTGGTTGGTGACGCGCAAGCCTTCTCAGTACTTCGCTTCAACGACCAGA
CCTTGGTCTCTGTCGAGTCGATCAACCGGCCCGGCGATCATCTGGCGGCCCGCCGCATACTGGCCGGCGACAAGCGCCCG
AGCCCCACCGATGCGCGTAGTCCGGGCTTCTCGCTCAAGTCGTTCGTGCAGTCCGCAACCAGTGCCGCGTAG

Upstream 100 bases:

>100_bases
AGGTTCAAGTCGACAACGCACTAGGGGCGATTACCGTCGAAATCCCCCTCGATACTGAATCCCCTGTCGCACAATCAGTG
CGGAAAGGCTAAGCTCCGCA

Downstream 100 bases:

>100_bases
TCGGCAGCGGACGCCGATCCACAAATGATGGCTTGTCTTGGACAACAGGGATACTAGTCAAACCGGGCACGTGAGTCGGC
ACGCGATCACTGAGCGGGCC

Product: pyridine nucleotide-disulphide oxidoreductase family protein

Products: oxidized ferredoxin; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MPALSDSMPVPPDREDAAVVIVGAGHAGVQVAVSLREFGFSDRIVIVDAEARSPYQRPPLSKEYLCGTVDQPPPLRAHEF
YEIQRIEFRRAAAVSIDRAQRTITLDDGSRLGYGHLVLATGARNREIPVPGANTPGVLGLRTLDDARDIRERIGTSDDIV
IIGAGFIGLEVAAAARKRGKRVHVVEAVDRVMARVVSPHVSQHLADLHAREGVNVHLGRSLAAIRTDRAGKLDTVELTCG
TTMAADLVVVGVGVVPNIELAAEAGLAVDNGVCVDAALLTKDPAISAVGDCAAFPVSGSGASIRLESVQNAVDHGRFVAA
RLTGSVENYVAVPWFWTHQYSAKLQIAGKALEVSESVVVGDAQAFSVLRFNDQTLVSVESINRPGDHLAARRILAGDKRP
SPTDARSPGFSLKSFVQSATSAA

Sequences:

>Translated_423_residues
MPALSDSMPVPPDREDAAVVIVGAGHAGVQVAVSLREFGFSDRIVIVDAEARSPYQRPPLSKEYLCGTVDQPPPLRAHEF
YEIQRIEFRRAAAVSIDRAQRTITLDDGSRLGYGHLVLATGARNREIPVPGANTPGVLGLRTLDDARDIRERIGTSDDIV
IIGAGFIGLEVAAAARKRGKRVHVVEAVDRVMARVVSPHVSQHLADLHAREGVNVHLGRSLAAIRTDRAGKLDTVELTCG
TTMAADLVVVGVGVVPNIELAAEAGLAVDNGVCVDAALLTKDPAISAVGDCAAFPVSGSGASIRLESVQNAVDHGRFVAA
RLTGSVENYVAVPWFWTHQYSAKLQIAGKALEVSESVVVGDAQAFSVLRFNDQTLVSVESINRPGDHLAARRILAGDKRP
SPTDARSPGFSLKSFVQSATSAA
>Mature_422_residues
PALSDSMPVPPDREDAAVVIVGAGHAGVQVAVSLREFGFSDRIVIVDAEARSPYQRPPLSKEYLCGTVDQPPPLRAHEFY
EIQRIEFRRAAAVSIDRAQRTITLDDGSRLGYGHLVLATGARNREIPVPGANTPGVLGLRTLDDARDIRERIGTSDDIVI
IGAGFIGLEVAAAARKRGKRVHVVEAVDRVMARVVSPHVSQHLADLHAREGVNVHLGRSLAAIRTDRAGKLDTVELTCGT
TMAADLVVVGVGVVPNIELAAEAGLAVDNGVCVDAALLTKDPAISAVGDCAAFPVSGSGASIRLESVQNAVDHGRFVAAR
LTGSVENYVAVPWFWTHQYSAKLQIAGKALEVSESVVVGDAQAFSVLRFNDQTLVSVESINRPGDHLAARRILAGDKRPS
PTDARSPGFSLKSFVQSATSAA

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=373, Percent_Identity=29.7587131367292, Blast_Score=165, Evalue=9e-41,
Organism=Homo sapiens, GI226437568, Length=373, Percent_Identity=29.7587131367292, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI21389617, Length=373, Percent_Identity=29.7587131367292, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI4757732, Length=331, Percent_Identity=28.0966767371601, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI22202629, Length=331, Percent_Identity=28.0966767371601, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1788892, Length=372, Percent_Identity=32.5268817204301, Blast_Score=164, Evalue=8e-42,
Organism=Escherichia coli, GI1789065, Length=276, Percent_Identity=28.9855072463768, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1789765, Length=254, Percent_Identity=27.5590551181102, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17559934, Length=369, Percent_Identity=26.2872628726287, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24639257, Length=355, Percent_Identity=27.3239436619718, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI281359715, Length=355, Percent_Identity=27.3239436619718, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI281359713, Length=355, Percent_Identity=27.3239436619718, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24639250, Length=355, Percent_Identity=27.3239436619718, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI18543267, Length=355, Percent_Identity=27.3239436619718, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24639252, Length=355, Percent_Identity=27.3239436619718, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI28573993, Length=373, Percent_Identity=27.0777479892761, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI24581020, Length=373, Percent_Identity=27.0777479892761, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24585130, Length=346, Percent_Identity=24.8554913294798, Blast_Score=109, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3

Molecular weight: Translated: 44796; Mature: 44665

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPALSDSMPVPPDREDAAVVIVGAGHAGVQVAVSLREFGFSDRIVIVDAEARSPYQRPPL
CCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEHHHCCCCCCEEEEECCCCCCCCCCCC
SKEYLCGTVDQPPPLRAHEFYEIQRIEFRRAAAVSIDRAQRTITLDDGSRLGYGHLVLAT
CHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCEEEEECCCCCCCCCEEEEEE
GARNREIPVPGANTPGVLGLRTLDDARDIRERIGTSDDIVIIGAGFIGLEVAAAARKRGK
CCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHCCC
RVHVVEAVDRVMARVVSPHVSQHLADLHAREGVNVHLGRSLAAIRTDRAGKLDTVELTCG
EEEHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCCCCEEEEEECC
TTMAADLVVVGVGVVPNIELAAEAGLAVDNGVCVDAALLTKDPAISAVGDCAAFPVSGSG
CHHHHCEEEEECCCCCCCEEHHCCCCEECCCCEEEEEEECCCCCCHHCCCCEECCCCCCC
ASIRLESVQNAVDHGRFVAARLTGSVENYVAVPWFWTHQYSAKLQIAGKALEVSESVVVG
CEEEHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEEECCCCEEEEEECCEEECCCEEEEE
DAQAFSVLRFNDQTLVSVESINRPGDHLAARRILAGDKRPSPTDARSPGFSLKSFVQSAT
CHHEEEEEEECCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
SAA
CCC
>Mature Secondary Structure 
PALSDSMPVPPDREDAAVVIVGAGHAGVQVAVSLREFGFSDRIVIVDAEARSPYQRPPL
CCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEHHHCCCCCCEEEEECCCCCCCCCCCC
SKEYLCGTVDQPPPLRAHEFYEIQRIEFRRAAAVSIDRAQRTITLDDGSRLGYGHLVLAT
CHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCEEEEECCCCCCCCCEEEEEE
GARNREIPVPGANTPGVLGLRTLDDARDIRERIGTSDDIVIIGAGFIGLEVAAAARKRGK
CCCCCCCCCCCCCCCCEEEEEECHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHCCC
RVHVVEAVDRVMARVVSPHVSQHLADLHAREGVNVHLGRSLAAIRTDRAGKLDTVELTCG
EEEHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCCCCEEEEEECC
TTMAADLVVVGVGVVPNIELAAEAGLAVDNGVCVDAALLTKDPAISAVGDCAAFPVSGSG
CHHHHCEEEEECCCCCCCEEHHCCCCEECCCCEEEEEEECCCCCCHHCCCCEECCCCCCC
ASIRLESVQNAVDHGRFVAARLTGSVENYVAVPWFWTHQYSAKLQIAGKALEVSESVVVG
CEEEHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEEECCCCEEEEEECCEEECCCEEEEE
DAQAFSVLRFNDQTLVSVESINRPGDHLAARRILAGDKRPSPTDARSPGFSLKSFVQSAT
CHHEEEEEEECCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
SAA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced ferredoxin; NAD+

Specific reaction: reduced ferredoxin + NAD+ = oxidized ferredoxin + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]