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The map label for this gene is yhjG [H]

Identifier: 169627956

GI number: 169627956

Start: 855899

End: 857368

Strand: Direct

Name: yhjG [H]

Synonym: MAB_0857

Alternate gene names: 169627956

Gene position: 855899-857368 (Clockwise)

Preceding gene: 169627954

Following gene: 169627957

Centisome position: 16.89

GC content: 64.56

Gene sequence:

>1470_bases
ATGGCTGAGCATGCGGTTGTTGTCGCGGGCGCAGGCCCGACCGGATTGATGTTGGCCGGTGAGCTGGCCCTTGCGGGCAT
CGATGTCGCTCTCCTGGAGCGCCGCGGCAGTCATGAGGAAGTGGGATCTCGTGCGGCGGGCCTGCATTCGCGCACCATCG
AAGTCTTCGATCAGCGGGGGATCGCAGAGCGATTCCTCGCCCAGGGACGTACCGGTCAGGTCACCCACTTTTCGGGCATT
TTCATGAGCATCGAGGACTTTCCGACCCGTCACCCCTACGCGCTGGGCCTACTGCAGCACTACACCGAGAAGACGCTGGC
GGCCTGGATCGACGAGCTCGGGGTACCGATCTATTACAACGCCGAGGCTGTTGGCCTCGGTCAGGACGACGTCGGCGTGG
ACGTGGCACTTGCCGACGGAAGCACCCTGCGGGGGCAGTACCTCGTCGGGTGTGACGGCGGGCGCAGCACCATCCGCAAG
GCGGCCGGGATCGGCTTTCCGGGACATGACCCGTCCTGCTCGGCGATGCTGGTCGATGCCGAAATCACCGAACAACCGCT
GGAGTTCGGCTTGCTGCGCAAGCCCGGGCAGGCGTTCCTCGGCATCCTGCCGTTCGAAGAAGGCTGGTGCCGGCTCGCCT
TCAGTACCGGCCTGGACCGGCTCGGCGTCGAACCGACGCTGGAAGAGGCCAAAGAAGCGCTGATCGCCATCGCGGGAACA
GATTTCGGAATACACAATCCGCGGTGGATATCCCGCTTCTCCGATATGACACGTCAGGCCGACAGGTATCGAGACGGGCG
GGTGTTCTTGGCCGGTGATTCCGCGCATATTCATTTCCCATTCGGCGGCCAGGGGCTGAACACCGGCATCCAGGACGCGG
TCAACCTGGGCTGGAAGCTTGCCGCGGTCATCAAGGGGCATGCCCCGGACGCGCTGCTGGACACCTATCACCTGGAGCGC
CACCCTGTCGCCGAACGGGTGCTGCACAACACCATGGCGCAAACGCCGTTGTCCGAAGCCGGTCCGCGGATCGACGCGTT
GCGTGACATCGTGGCGGACCTGCTGGCCATGGACGAACCGCGCAAGCGCATCGCCGGAATGATGAGCGGGCTGGACATCC
ACTACATGTTGGGCGACGGACATCAACTGTTGGGCCGCCGGATGCCCGACCTCGACGTGGTGACGGCGGACGGGGCGGTG
CGGGTTTACGAACATCTGCACACCGCGCGCCCCGTGTTGTTCAACTTCGGGGCTCGGCAGATCCTGAATGTCGAGGGATG
GGAAGATCGAGTACACGTGGTCGACGCCAAATATTCAGGAATATGGGAACTTCCGGTGTTGGGTGAGGTGTCGGCGCCGG
ATGCGGTGCTCGTGCGGCCCGACGGGTATGTGGCGTGGGTGGGCGAGGGCAGTGCGGATGGACTTCGCGGCGCGCTGACC
CGATGGGTGGGATCACCTTCTCGGGCGTAG

Upstream 100 bases:

>100_bases
CCGGCGCGTGCGATCGTCTCTCGATTCAAAATCACGTTGACACTTTAACATCGTTAAAGACATTATGGTGTTAATGCCGA
TAACGATGTTAAGGAGTGTT

Downstream 100 bases:

>100_bases
TGTGGCCCAGGTGGCCGAGACACTGGAGTTCGGAGTCTTCATCCCGCAAGGCTGGCGTCTGGATTTGGTAGGCATCGCCC
CGGAGCGTCATTGGGACGTG

Product: hypothetical protein

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MAEHAVVVAGAGPTGLMLAGELALAGIDVALLERRGSHEEVGSRAAGLHSRTIEVFDQRGIAERFLAQGRTGQVTHFSGI
FMSIEDFPTRHPYALGLLQHYTEKTLAAWIDELGVPIYYNAEAVGLGQDDVGVDVALADGSTLRGQYLVGCDGGRSTIRK
AAGIGFPGHDPSCSAMLVDAEITEQPLEFGLLRKPGQAFLGILPFEEGWCRLAFSTGLDRLGVEPTLEEAKEALIAIAGT
DFGIHNPRWISRFSDMTRQADRYRDGRVFLAGDSAHIHFPFGGQGLNTGIQDAVNLGWKLAAVIKGHAPDALLDTYHLER
HPVAERVLHNTMAQTPLSEAGPRIDALRDIVADLLAMDEPRKRIAGMMSGLDIHYMLGDGHQLLGRRMPDLDVVTADGAV
RVYEHLHTARPVLFNFGARQILNVEGWEDRVHVVDAKYSGIWELPVLGEVSAPDAVLVRPDGYVAWVGEGSADGLRGALT
RWVGSPSRA

Sequences:

>Translated_489_residues
MAEHAVVVAGAGPTGLMLAGELALAGIDVALLERRGSHEEVGSRAAGLHSRTIEVFDQRGIAERFLAQGRTGQVTHFSGI
FMSIEDFPTRHPYALGLLQHYTEKTLAAWIDELGVPIYYNAEAVGLGQDDVGVDVALADGSTLRGQYLVGCDGGRSTIRK
AAGIGFPGHDPSCSAMLVDAEITEQPLEFGLLRKPGQAFLGILPFEEGWCRLAFSTGLDRLGVEPTLEEAKEALIAIAGT
DFGIHNPRWISRFSDMTRQADRYRDGRVFLAGDSAHIHFPFGGQGLNTGIQDAVNLGWKLAAVIKGHAPDALLDTYHLER
HPVAERVLHNTMAQTPLSEAGPRIDALRDIVADLLAMDEPRKRIAGMMSGLDIHYMLGDGHQLLGRRMPDLDVVTADGAV
RVYEHLHTARPVLFNFGARQILNVEGWEDRVHVVDAKYSGIWELPVLGEVSAPDAVLVRPDGYVAWVGEGSADGLRGALT
RWVGSPSRA
>Mature_488_residues
AEHAVVVAGAGPTGLMLAGELALAGIDVALLERRGSHEEVGSRAAGLHSRTIEVFDQRGIAERFLAQGRTGQVTHFSGIF
MSIEDFPTRHPYALGLLQHYTEKTLAAWIDELGVPIYYNAEAVGLGQDDVGVDVALADGSTLRGQYLVGCDGGRSTIRKA
AGIGFPGHDPSCSAMLVDAEITEQPLEFGLLRKPGQAFLGILPFEEGWCRLAFSTGLDRLGVEPTLEEAKEALIAIAGTD
FGIHNPRWISRFSDMTRQADRYRDGRVFLAGDSAHIHFPFGGQGLNTGIQDAVNLGWKLAAVIKGHAPDALLDTYHLERH
PVAERVLHNTMAQTPLSEAGPRIDALRDIVADLLAMDEPRKRIAGMMSGLDIHYMLGDGHQLLGRRMPDLDVVTADGAVR
VYEHLHTARPVLFNFGARQILNVEGWEDRVHVVDAKYSGIWELPVLGEVSAPDAVLVRPDGYVAWVGEGSADGLRGALTR
WVGSPSRA

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=379, Percent_Identity=29.8153034300792, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 52889; Mature: 52758

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEHAVVVAGAGPTGLMLAGELALAGIDVALLERRGSHEEVGSRAAGLHSRTIEVFDQRG
CCCCEEEEECCCCCCEEEECCHHHHCCHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCC
IAERFLAQGRTGQVTHFSGIFMSIEDFPTRHPYALGLLQHYTEKTLAAWIDELGVPIYYN
HHHHHHHCCCCCCEEEECEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEC
AEAVGLGQDDVGVDVALADGSTLRGQYLVGCDGGRSTIRKAAGIGFPGHDPSCSAMLVDA
CEEEECCCCCCCEEEEEECCCEECCCEEEECCCCHHHHHHHHCCCCCCCCCCCCEEEEEC
EITEQPLEFGLLRKPGQAFLGILPFEEGWCRLAFSTGLDRLGVEPTLEEAKEALIAIAGT
HHHHCHHHHHCCCCCCCCEEEEEECCCCCEEEEHHCCCHHCCCCCCHHHHHHEEEEEECC
DFGIHNPRWISRFSDMTRQADRYRDGRVFLAGDSAHIHFPFGGQGLNTGIQDAVNLGWKL
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEECCCCCCCCCCHHHHHHCCEEE
AAVIKGHAPDALLDTYHLERHPVAERVLHNTMAQTPLSEAGPRIDALRDIVADLLAMDEP
EEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHCCCH
RKRIAGMMSGLDIHYMLGDGHQLLGRRMPDLDVVTADGAVRVYEHLHTARPVLFNFGARQ
HHHHHHHHCCCEEEEEECCCHHHHHCCCCCCEEEECCCHHHHHHHHHHCCCCEEECCCHH
ILNVEGWEDRVHVVDAKYSGIWELPVLGEVSAPDAVLVRPDGYVAWVGEGSADGLRGALT
EEECCCCCCEEEEEEECCCCEEECCCCCCCCCCCEEEECCCCEEEEECCCCCCHHHHHHH
RWVGSPSRA
HHCCCCCCC
>Mature Secondary Structure 
AEHAVVVAGAGPTGLMLAGELALAGIDVALLERRGSHEEVGSRAAGLHSRTIEVFDQRG
CCCEEEEECCCCCCEEEECCHHHHCCHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCC
IAERFLAQGRTGQVTHFSGIFMSIEDFPTRHPYALGLLQHYTEKTLAAWIDELGVPIYYN
HHHHHHHCCCCCCEEEECEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEC
AEAVGLGQDDVGVDVALADGSTLRGQYLVGCDGGRSTIRKAAGIGFPGHDPSCSAMLVDA
CEEEECCCCCCCEEEEEECCCEECCCEEEECCCCHHHHHHHHCCCCCCCCCCCCEEEEEC
EITEQPLEFGLLRKPGQAFLGILPFEEGWCRLAFSTGLDRLGVEPTLEEAKEALIAIAGT
HHHHCHHHHHCCCCCCCCEEEEEECCCCCEEEEHHCCCHHCCCCCCHHHHHHEEEEEECC
DFGIHNPRWISRFSDMTRQADRYRDGRVFLAGDSAHIHFPFGGQGLNTGIQDAVNLGWKL
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEECCCCCCCCCCHHHHHHCCEEE
AAVIKGHAPDALLDTYHLERHPVAERVLHNTMAQTPLSEAGPRIDALRDIVADLLAMDEP
EEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHCCCH
RKRIAGMMSGLDIHYMLGDGHQLLGRRMPDLDVVTADGAVRVYEHLHTARPVLFNFGARQ
HHHHHHHHCCCEEEEEECCCHHHHHCCCCCCEEEECCCHHHHHHHHHHCCCCEEECCCHH
ILNVEGWEDRVHVVDAKYSGIWELPVLGEVSAPDAVLVRPDGYVAWVGEGSADGLRGALT
EEECCCCCCEEEEEEECCCCEEECCCCCCCCCCCEEEECCCCEEEEECCCCCCHHHHHHH
RWVGSPSRA
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]