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The map label for this gene is fadJ [H]

Identifier: 169627950

GI number: 169627950

Start: 845430

End: 847589

Strand: Direct

Name: fadJ [H]

Synonym: MAB_0851

Alternate gene names: 169627950

Gene position: 845430-847589 (Clockwise)

Preceding gene: 169627949

Following gene: 169627951

Centisome position: 16.68

GC content: 64.95

Gene sequence:

>2160_bases
ATGTCTGCAAACACCATTCAGTGGGAGAAGGACGCCGACGGCATCGTCACCCTCACCCTGGACGACCCCAACGGCTCGGC
CAACGTGATGAACGACGACTACAAGCAGTCGATGGCCGCGGCCGTCAAGCGCCTTGTGGAAGAGAAGGATTCGATCACCG
GCGTGGTGATCACCAGCGCCAAGAAGACCTTCTTCGCCGGTGGTGACCTGACCAAGATCATCCAGATCCAGCCGGAGAAC
GCCCAGGAGGCCTTCAACGAGGTCGAGGAGATCAAGGCCGACCTGCGTACGCTGGAGACCTTCGGCCGCCCCGTGGTGGC
GGCCATCAACGGCGCCGCCCTCGGCGGTGGCCTGGAGATCGCGCTGGCGACCCATCACCGGATCGCGGCCGACGTCAAGG
GATCTCAGATCGGTCTGCCCGAGGTATCGCTGGGCCTGCTGCCCGGTGGCGGCGGCGTCACCCGGGTTACCCGCATGCTC
GGTATCCAGAACGGCTTCGTGACCGTGCTGGCGCAGGGCACCCGCTTCAACCCGACCAAGGCCAAGGAAGTCGGCTTGAT
CGATGAGTTGGTCGGCTCCGTCGACGAGCTGATCCCGGCCGCCAAGGCGTGGATCAAGGCCAACCCGGAGGCCGTGCAGC
GCTGGGATGTCAAGGGCTACAAGATCCCCGGCGGCACCCCAGCAACTCCCGCCCTGGCCGCGATTTTGCCGTCGTTCCCG
TCGAACCTGAAGAAGCAGCTCAAGGGTGCGCCGATGCCCGCCCCGCGGGCCATCCTGGCCGCCGCGGTCGAGGGTGCGCA
GGTGGACTTCGACACGGCCAGCCGCATCGAGAGCCGTTACTTCACCAGCCTGGCCACCGGCCAGGTCGCCAAGAACATGA
CGCAGGCGTTCTTCTTCGACCTGCAGCACATCAACGGCGGTGGCTCACGTCCGGACGGCATCGCCAAGCAAGACATCAAG
AAGATCGGTGTGCTGGGCGCGGGCATGATGGGCGCCGGTATCGCCTACGTCTCGGCCAAGGCCGGTTACGACGTCGTGCT
CAAGGATGTTTCGCTGGAGGCGGCTCAGAAGGGCAAGGGTTACTCCGAAAAGCTTGAAGAGAAGGCTCTTTCGCGGGGCA
AGACCACCCAGGAAAAGTCCAGCGAACTCCTCGCAAGAATTCACCCGACTGCCGACCCTGCCGACCTGGCCGGTGTCGAC
TTCGTGATCGAGGCCGTTTTCGAGAACACCGAGCTCAAGCACAAGGTGTTCCAGGAGATCGAAGACATCGTCGAGCCCAA
CGCGCTCCTTGGCTCCAACACCTCCACCCTGCCCATCACGGGGCTGGCCACGGGTGTGAAGCGTCAGGAGGACTTCATCG
GTATCCACTTCTTCTCGCCCGTGGACAAGATGCCGCTGGTGGAGATCATCCGCGGTGAGAAGACCTCGGACGAGGCGCTG
GCCCGGGTGTTCGACTACGTGCTGGCCATCAAGAAGACCCCGATCGTCGTCAATGACAGCCGTGGCTTCTTCACCAGCCG
CGTCATCGGCACCTTCGTCAACGAGGCCGTCGCCATGCTGGCCGAGGGCATCGAGCCCGCGACCATCGAGCAGGCCGGCA
GCCAGGCCGGATACCCGGCCCCGCCGCTGCAGCTCTCCGACGAGCTGAACCTGACGCTCATGCAGAAGATCCGCAAGGAG
ACGGCGGAGGCTGCCAAGGCCGAGGGCAAGGAATTGCCGGATGACCCGGCCGGTCGTGTCATCGACACCCTGGTTGAGGC
GGGCCGCCCCGGCCGCCTCGGGGGCGCCGGTTTCTACGACTACGTGGACGGCAAGCGCACAGAGCTGTGGCCCGGCCTGC
GGGAGACGTTCAAGACCCGCAATGGCGCCGACCCCGCCAACCCGCCGCTGCAGGACCTCATCGAGCGCATGTTGTTCGCC
GAGGCCATCGAGACGCAGAAGTGCTTCGACGAGGGTGTGCTGACGTCGACCGCCGATGCCAACATCGGTTCGATCTTCGG
CATCGGCTTCCCGGCCTGGACCGGTGGTGTGCACCAGTACATCCTCGGGTACGACGGTCCCGCCGGAAAGGGCAAGGCTG
GTTTCGTCGCCCGCGCAAAGGAATTGGCCGCCAAGTACGGTGACCGATTCAACCCGCCTGCTTCGCTCCTGGACGCATAG

Upstream 100 bases:

>100_bases
CGTCGCGGCCTCAAGCGTGCCCTCATCACCCTGTGCGTCGGCGGCGGCATGGGTGTAGCCACCATCATCGAGCGAGTCTG
AGCTAAGGGAGCGAGAACTT

Downstream 100 bases:

>100_bases
CAGGTCGAAACAATCCCCTCAGCCGCTCACCCTTTCGGGTGGGCGGCTGAGGTCGTTTCAGGGCCGTTTTCGGGTATGCG
CCGAGCAGTTCGCCCGGGGG

Product: fatty oxidation protein FadB

Products: NA

Alternate protein names: Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimerase; 3-hydroxyacyl-CoA dehydrogenase [H]

Number of amino acids: Translated: 719; Mature: 718

Protein sequence:

>719_residues
MSANTIQWEKDADGIVTLTLDDPNGSANVMNDDYKQSMAAAVKRLVEEKDSITGVVITSAKKTFFAGGDLTKIIQIQPEN
AQEAFNEVEEIKADLRTLETFGRPVVAAINGAALGGGLEIALATHHRIAADVKGSQIGLPEVSLGLLPGGGGVTRVTRML
GIQNGFVTVLAQGTRFNPTKAKEVGLIDELVGSVDELIPAAKAWIKANPEAVQRWDVKGYKIPGGTPATPALAAILPSFP
SNLKKQLKGAPMPAPRAILAAAVEGAQVDFDTASRIESRYFTSLATGQVAKNMTQAFFFDLQHINGGGSRPDGIAKQDIK
KIGVLGAGMMGAGIAYVSAKAGYDVVLKDVSLEAAQKGKGYSEKLEEKALSRGKTTQEKSSELLARIHPTADPADLAGVD
FVIEAVFENTELKHKVFQEIEDIVEPNALLGSNTSTLPITGLATGVKRQEDFIGIHFFSPVDKMPLVEIIRGEKTSDEAL
ARVFDYVLAIKKTPIVVNDSRGFFTSRVIGTFVNEAVAMLAEGIEPATIEQAGSQAGYPAPPLQLSDELNLTLMQKIRKE
TAEAAKAEGKELPDDPAGRVIDTLVEAGRPGRLGGAGFYDYVDGKRTELWPGLRETFKTRNGADPANPPLQDLIERMLFA
EAIETQKCFDEGVLTSTADANIGSIFGIGFPAWTGGVHQYILGYDGPAGKGKAGFVARAKELAAKYGDRFNPPASLLDA

Sequences:

>Translated_719_residues
MSANTIQWEKDADGIVTLTLDDPNGSANVMNDDYKQSMAAAVKRLVEEKDSITGVVITSAKKTFFAGGDLTKIIQIQPEN
AQEAFNEVEEIKADLRTLETFGRPVVAAINGAALGGGLEIALATHHRIAADVKGSQIGLPEVSLGLLPGGGGVTRVTRML
GIQNGFVTVLAQGTRFNPTKAKEVGLIDELVGSVDELIPAAKAWIKANPEAVQRWDVKGYKIPGGTPATPALAAILPSFP
SNLKKQLKGAPMPAPRAILAAAVEGAQVDFDTASRIESRYFTSLATGQVAKNMTQAFFFDLQHINGGGSRPDGIAKQDIK
KIGVLGAGMMGAGIAYVSAKAGYDVVLKDVSLEAAQKGKGYSEKLEEKALSRGKTTQEKSSELLARIHPTADPADLAGVD
FVIEAVFENTELKHKVFQEIEDIVEPNALLGSNTSTLPITGLATGVKRQEDFIGIHFFSPVDKMPLVEIIRGEKTSDEAL
ARVFDYVLAIKKTPIVVNDSRGFFTSRVIGTFVNEAVAMLAEGIEPATIEQAGSQAGYPAPPLQLSDELNLTLMQKIRKE
TAEAAKAEGKELPDDPAGRVIDTLVEAGRPGRLGGAGFYDYVDGKRTELWPGLRETFKTRNGADPANPPLQDLIERMLFA
EAIETQKCFDEGVLTSTADANIGSIFGIGFPAWTGGVHQYILGYDGPAGKGKAGFVARAKELAAKYGDRFNPPASLLDA
>Mature_718_residues
SANTIQWEKDADGIVTLTLDDPNGSANVMNDDYKQSMAAAVKRLVEEKDSITGVVITSAKKTFFAGGDLTKIIQIQPENA
QEAFNEVEEIKADLRTLETFGRPVVAAINGAALGGGLEIALATHHRIAADVKGSQIGLPEVSLGLLPGGGGVTRVTRMLG
IQNGFVTVLAQGTRFNPTKAKEVGLIDELVGSVDELIPAAKAWIKANPEAVQRWDVKGYKIPGGTPATPALAAILPSFPS
NLKKQLKGAPMPAPRAILAAAVEGAQVDFDTASRIESRYFTSLATGQVAKNMTQAFFFDLQHINGGGSRPDGIAKQDIKK
IGVLGAGMMGAGIAYVSAKAGYDVVLKDVSLEAAQKGKGYSEKLEEKALSRGKTTQEKSSELLARIHPTADPADLAGVDF
VIEAVFENTELKHKVFQEIEDIVEPNALLGSNTSTLPITGLATGVKRQEDFIGIHFFSPVDKMPLVEIIRGEKTSDEALA
RVFDYVLAIKKTPIVVNDSRGFFTSRVIGTFVNEAVAMLAEGIEPATIEQAGSQAGYPAPPLQLSDELNLTLMQKIRKET
AEAAKAEGKELPDDPAGRVIDTLVEAGRPGRLGGAGFYDYVDGKRTELWPGLRETFKTRNGADPANPPLQDLIERMLFAE
AIETQKCFDEGVLTSTADANIGSIFGIGFPAWTGGVHQYILGYDGPAGKGKAGFVARAKELAAKYGDRFNPPASLLDA

Specific function: Catalyzes the formation of an hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities [H]

COG id: COG1250

COG function: function code I; 3-hydroxyacyl-CoA dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the central section; belongs to the 3-hydroxyacyl- CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI20127408, Length=738, Percent_Identity=30.3523035230352, Blast_Score=305, Evalue=1e-82,
Organism=Homo sapiens, GI68989263, Length=701, Percent_Identity=28.2453637660485, Blast_Score=226, Evalue=5e-59,
Organism=Homo sapiens, GI261878539, Length=588, Percent_Identity=28.9115646258503, Blast_Score=207, Evalue=3e-53,
Organism=Homo sapiens, GI296179429, Length=293, Percent_Identity=31.740614334471, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI296179427, Length=308, Percent_Identity=29.5454545454545, Blast_Score=133, Evalue=7e-31,
Organism=Homo sapiens, GI194097323, Length=174, Percent_Identity=29.8850574712644, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI115430219, Length=208, Percent_Identity=25.9615384615385, Blast_Score=69, Evalue=2e-11,
Organism=Homo sapiens, GI157694516, Length=210, Percent_Identity=27.6190476190476, Blast_Score=68, Evalue=4e-11,
Organism=Homo sapiens, GI213417737, Length=210, Percent_Identity=27.6190476190476, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1788682, Length=723, Percent_Identity=33.7482710926694, Blast_Score=308, Evalue=7e-85,
Organism=Escherichia coli, GI1790281, Length=683, Percent_Identity=33.0893118594436, Blast_Score=301, Evalue=9e-83,
Organism=Escherichia coli, GI1787661, Length=287, Percent_Identity=34.1463414634146, Blast_Score=156, Evalue=4e-39,
Organism=Escherichia coli, GI1787659, Length=209, Percent_Identity=29.1866028708134, Blast_Score=76, Evalue=1e-14,
Organism=Escherichia coli, GI221142681, Length=190, Percent_Identity=29.4736842105263, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17558304, Length=740, Percent_Identity=31.2162162162162, Blast_Score=305, Evalue=4e-83,
Organism=Caenorhabditis elegans, GI17508951, Length=706, Percent_Identity=33.0028328611898, Blast_Score=297, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI17508953, Length=706, Percent_Identity=33.0028328611898, Blast_Score=296, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI25144276, Length=623, Percent_Identity=33.5473515248796, Blast_Score=265, Evalue=8e-71,
Organism=Caenorhabditis elegans, GI71985923, Length=364, Percent_Identity=28.5714285714286, Blast_Score=169, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI71985930, Length=364, Percent_Identity=26.6483516483516, Blast_Score=159, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17553560, Length=295, Percent_Identity=33.2203389830508, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17549919, Length=293, Percent_Identity=33.1058020477816, Blast_Score=147, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17563036, Length=294, Percent_Identity=31.2925170068027, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17540714, Length=208, Percent_Identity=28.8461538461538, Blast_Score=75, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25145438, Length=181, Percent_Identity=28.7292817679558, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24583077, Length=740, Percent_Identity=31.6216216216216, Blast_Score=300, Evalue=2e-81,
Organism=Drosophila melanogaster, GI24583079, Length=740, Percent_Identity=31.6216216216216, Blast_Score=300, Evalue=2e-81,
Organism=Drosophila melanogaster, GI19921000, Length=740, Percent_Identity=31.6216216216216, Blast_Score=300, Evalue=2e-81,
Organism=Drosophila melanogaster, GI45549573, Length=211, Percent_Identity=28.9099526066351, Blast_Score=86, Evalue=9e-17,
Organism=Drosophila melanogaster, GI20129993, Length=212, Percent_Identity=29.7169811320755, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24653139, Length=183, Percent_Identity=33.879781420765, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI20129971, Length=183, Percent_Identity=30.0546448087432, Blast_Score=77, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24653477, Length=183, Percent_Identity=30.0546448087432, Blast_Score=77, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006180
- InterPro:   IPR006176
- InterPro:   IPR006108
- InterPro:   IPR008927
- InterPro:   IPR001753
- InterPro:   IPR013328
- InterPro:   IPR012802
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00725 3HCDH; PF02737 3HCDH_N; PF00378 ECH [H]

EC number: =4.2.1.17; =5.1.2.3; =1.1.1.35 [H]

Molecular weight: Translated: 76500; Mature: 76369

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSANTIQWEKDADGIVTLTLDDPNGSANVMNDDYKQSMAAAVKRLVEEKDSITGVVITSA
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECC
KKTFFAGGDLTKIIQIQPENAQEAFNEVEEIKADLRTLETFGRPVVAAINGAALGGGLEI
CCEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEECCCEEE
ALATHHRIAADVKGSQIGLPEVSLGLLPGGGGVTRVTRMLGIQNGFVTVLAQGTRFNPTK
EEECCHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCC
AKEVGLIDELVGSVDELIPAAKAWIKANPEAVQRWDVKGYKIPGGTPATPALAAILPSFP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEECCCCCCCHHHHHHHHHHCH
SNLKKQLKGAPMPAPRAILAAAVEGAQVDFDTASRIESRYFTSLATGQVAKNMTQAFFFD
HHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQHINGGGSRPDGIAKQDIKKIGVLGAGMMGAGIAYVSAKAGYDVVLKDVSLEAAQKGKG
HHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHCCCCEEECCCCCEEEEECCCHHHHHCCCC
YSEKLEEKALSRGKTTQEKSSELLARIHPTADPADLAGVDFVIEAVFENTELKHKVFQEI
HHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
EDIVEPNALLGSNTSTLPITGLATGVKRQEDFIGIHFFSPVDKMPLVEIIRGEKTSDEAL
HHHHCCCCEECCCCCCEEEHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHCCCCCCHHHH
ARVFDYVLAIKKTPIVVNDSRGFFTSRVIGTFVNEAVAMLAEGIEPATIEQAGSQAGYPA
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCC
PPLQLSDELNLTLMQKIRKETAEAAKAEGKELPDDPAGRVIDTLVEAGRPGRLGGAGFYD
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHH
YVDGKRTELWPGLRETFKTRNGADPANPPLQDLIERMLFAEAIETQKCFDEGVLTSTADA
CCCCCCHHHCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
NIGSIFGIGFPAWTGGVHQYILGYDGPAGKGKAGFVARAKELAAKYGDRFNPPASLLDA
CCCCEEECCCCHHCCCHHHEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHCC
>Mature Secondary Structure 
SANTIQWEKDADGIVTLTLDDPNGSANVMNDDYKQSMAAAVKRLVEEKDSITGVVITSA
CCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECC
KKTFFAGGDLTKIIQIQPENAQEAFNEVEEIKADLRTLETFGRPVVAAINGAALGGGLEI
CCEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEECCCEEE
ALATHHRIAADVKGSQIGLPEVSLGLLPGGGGVTRVTRMLGIQNGFVTVLAQGTRFNPTK
EEECCHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCC
AKEVGLIDELVGSVDELIPAAKAWIKANPEAVQRWDVKGYKIPGGTPATPALAAILPSFP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEECCCCCCCHHHHHHHHHHCH
SNLKKQLKGAPMPAPRAILAAAVEGAQVDFDTASRIESRYFTSLATGQVAKNMTQAFFFD
HHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQHINGGGSRPDGIAKQDIKKIGVLGAGMMGAGIAYVSAKAGYDVVLKDVSLEAAQKGKG
HHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHCCCCEEECCCCCEEEEECCCHHHHHCCCC
YSEKLEEKALSRGKTTQEKSSELLARIHPTADPADLAGVDFVIEAVFENTELKHKVFQEI
HHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
EDIVEPNALLGSNTSTLPITGLATGVKRQEDFIGIHFFSPVDKMPLVEIIRGEKTSDEAL
HHHHCCCCEECCCCCCEEEHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHCCCCCCHHHH
ARVFDYVLAIKKTPIVVNDSRGFFTSRVIGTFVNEAVAMLAEGIEPATIEQAGSQAGYPA
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCC
PPLQLSDELNLTLMQKIRKETAEAAKAEGKELPDDPAGRVIDTLVEAGRPGRLGGAGFYD
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHH
YVDGKRTELWPGLRETFKTRNGADPANPPLQDLIERMLFAEAIETQKCFDEGVLTSTADA
CCCCCCHHHCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
NIGSIFGIGFPAWTGGVHQYILGYDGPAGKGKAGFVARAKELAAKYGDRFNPPASLLDA
CCCCEEECCCCHHCCCHHHEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA