Click here to switch to the map view.

The map label for this gene is dus [H]

Identifier: 169627854

GI number: 169627854

Start: 754360

End: 755535

Strand: Reverse

Name: dus [H]

Synonym: MAB_0753c

Alternate gene names: 169627854

Gene position: 755535-754360 (Counterclockwise)

Preceding gene: 169627855

Following gene: 169627853

Centisome position: 14.91

GC content: 65.9

Gene sequence:

>1176_bases
GTGACCGCATCTGTCGACCTACCAGAGGCCCGATCCCGCGAACTGCGGATCGGGTCTTTGGCATTGCCCAGCCCGGTGGT
GCTGGCCCCGATGGCCGGTGTCACCAACGTCGCGTTCCGAACGCTGTGCCGCGAGCTCGAACTGGCCACCACGGGAACCG
TCAGCGGGCTGTACGTCTGTGAGATGGTCACCGCCCGTGCGCTCGCCGAGCGGCATCCGGTGACGCTGCACATGACCACC
TTCTCGCCGGAAGAGTCGCCACGATCCCTGCAGCTGTATTCGGTCGATCCGGAGACCACCTACCGCGCCGCCAAGATGGT
GGTCGACGAAGGCCTCGCCGATCACATCGACATGAATTTCGGCTGCCCGGTACCCAAGGTCACCAGAAACGGCGGTGGCG
CCGCCCTTCCCTACAAACGGCGGCTGTTCGGGCAGATCGTGGCCGCCGCCGTGCGTGCCACCGCGGGGACCGATATCCCG
GTGACGGTCAAATTCCGCGTCGGCATCGACGACGACCACCACACCCACCTGGACGCCGGCGCCATCGCCGAGTCGGAGGG
GGCCGCAGCCGTCGCGCTGCATGCCCGCACCGCCTCACAGCGCTACTCGGGCACCGCCGACTGGAGCCAGATTGCCGCGC
TCAAGAACCACGTGACAACCATCCCCGTCCTGGGGAACGGCGATATATTCGACGCCGCCGACGCGGTCGCGATGATGGAA
CAGACCGGTTGCGACGGCGTCGTCATCGGCCGCGGCTGTCTCGGACGTCCGTGGCTCTTTGCCGAATTGAGTGCCGTTTT
CAACGGTCAGACGATTCCGGCGCCTCCGAATCTCGGTCAGGTAACGGTGATTATGCGTCGTCACGCGGAGTTGCTCGTCG
ACCATTTTGGGGAAGACAAGGCGTTGCGCGACATGCGCAAGCACATCGCCTGGTATCTCCACGGCTTCCCCGCCGGTGGT
GACATCCGGCGGGCGCTCGCGCTCGTGAGCACCCGCGCCGAGCTGGACACCCTGCTCGAACAGCTCGATCAATCCGCGCC
CTTCCCTGAGGGCGGAAACGGACCTCGCGGACGCCAGGGGTCACCCGCGAAGGTGTCGCTACCCGATGGCTGGCTCAATG
ACCCCGACGATTGCACAGTTCCCTCAGCAGCCGACGTCATGCACTCTGGTGGATGA

Upstream 100 bases:

>100_bases
CTTCGAAGAGGTCAAGGCCAAGTACCTGGAACGCCAGGCCAAGCGCGCAGAACGCCAGGCCGCCAAGGCTGCCCGGGAAG
CTGTCAGCGTCTAACCGGCT

Downstream 100 bases:

>100_bases
CGCGGGGTCACATCTAGGTCACCGGGCATGGTGATCTGCGGCAATGCTCAGTAGCATGTGACGACGACCGTGCCCCGGGC
ACGGCGTTATCTGCTGCCGG

Product: tRNA-dihydrouridine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MTASVDLPEARSRELRIGSLALPSPVVLAPMAGVTNVAFRTLCRELELATTGTVSGLYVCEMVTARALAERHPVTLHMTT
FSPEESPRSLQLYSVDPETTYRAAKMVVDEGLADHIDMNFGCPVPKVTRNGGGAALPYKRRLFGQIVAAAVRATAGTDIP
VTVKFRVGIDDDHHTHLDAGAIAESEGAAAVALHARTASQRYSGTADWSQIAALKNHVTTIPVLGNGDIFDAADAVAMME
QTGCDGVVIGRGCLGRPWLFAELSAVFNGQTIPAPPNLGQVTVIMRRHAELLVDHFGEDKALRDMRKHIAWYLHGFPAGG
DIRRALALVSTRAELDTLLEQLDQSAPFPEGGNGPRGRQGSPAKVSLPDGWLNDPDDCTVPSAADVMHSGG

Sequences:

>Translated_391_residues
MTASVDLPEARSRELRIGSLALPSPVVLAPMAGVTNVAFRTLCRELELATTGTVSGLYVCEMVTARALAERHPVTLHMTT
FSPEESPRSLQLYSVDPETTYRAAKMVVDEGLADHIDMNFGCPVPKVTRNGGGAALPYKRRLFGQIVAAAVRATAGTDIP
VTVKFRVGIDDDHHTHLDAGAIAESEGAAAVALHARTASQRYSGTADWSQIAALKNHVTTIPVLGNGDIFDAADAVAMME
QTGCDGVVIGRGCLGRPWLFAELSAVFNGQTIPAPPNLGQVTVIMRRHAELLVDHFGEDKALRDMRKHIAWYLHGFPAGG
DIRRALALVSTRAELDTLLEQLDQSAPFPEGGNGPRGRQGSPAKVSLPDGWLNDPDDCTVPSAADVMHSGG
>Mature_390_residues
TASVDLPEARSRELRIGSLALPSPVVLAPMAGVTNVAFRTLCRELELATTGTVSGLYVCEMVTARALAERHPVTLHMTTF
SPEESPRSLQLYSVDPETTYRAAKMVVDEGLADHIDMNFGCPVPKVTRNGGGAALPYKRRLFGQIVAAAVRATAGTDIPV
TVKFRVGIDDDHHTHLDAGAIAESEGAAAVALHARTASQRYSGTADWSQIAALKNHVTTIPVLGNGDIFDAADAVAMMEQ
TGCDGVVIGRGCLGRPWLFAELSAVFNGQTIPAPPNLGQVTVIMRRHAELLVDHFGEDKALRDMRKHIAWYLHGFPAGGD
IRRALALVSTRAELDTLLEQLDQSAPFPEGGNGPRGRQGSPAKVSLPDGWLNDPDDCTVPSAADVMHSGG

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=318, Percent_Identity=28.9308176100629, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI239788462, Length=304, Percent_Identity=28.9473684210526, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI31742496, Length=235, Percent_Identity=29.7872340425532, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI40807366, Length=362, Percent_Identity=27.3480662983425, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI1789660, Length=328, Percent_Identity=34.7560975609756, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1788462, Length=269, Percent_Identity=30.4832713754647, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI145693211, Length=322, Percent_Identity=27.3291925465839, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17543114, Length=306, Percent_Identity=27.7777777777778, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI25144369, Length=231, Percent_Identity=29.4372294372294, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17507177, Length=251, Percent_Identity=27.4900398406374, Blast_Score=71, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=322, Percent_Identity=27.9503105590062, Blast_Score=93, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6323437, Length=240, Percent_Identity=27.5, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323433, Length=229, Percent_Identity=25.3275109170306, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24585320, Length=281, Percent_Identity=30.9608540925267, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24580595, Length=249, Percent_Identity=29.3172690763052, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI19920448, Length=249, Percent_Identity=29.3172690763052, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI19921524, Length=251, Percent_Identity=29.0836653386454, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 41639; Mature: 41508

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASVDLPEARSRELRIGSLALPSPVVLAPMAGVTNVAFRTLCRELELATTGTVSGLYVC
CCCCCCCCCCCCCCEEECCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHH
EMVTARALAERHPVTLHMTTFSPEESPRSLQLYSVDPETTYRAAKMVVDEGLADHIDMNF
HHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCHHHCCCCC
GCPVPKVTRNGGGAALPYKRRLFGQIVAAAVRATAGTDIPVTVKFRVGIDDDHHTHLDAG
CCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCC
AIAESEGAAAVALHARTASQRYSGTADWSQIAALKNHVTTIPVLGNGDIFDAADAVAMME
CCCCCCCCEEEEEEEHHHHHHCCCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHH
QTGCDGVVIGRGCLGRPWLFAELSAVFNGQTIPAPPNLGQVTVIMRRHAELLVDHFGEDK
HCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCH
ALRDMRKHIAWYLHGFPAGGDIRRALALVSTRAELDTLLEQLDQSAPFPEGGNGPRGRQG
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
SPAKVSLPDGWLNDPDDCTVPSAADVMHSGG
CCCEEECCCCCCCCCCCCCCCCHHHHHHCCC
>Mature Secondary Structure 
TASVDLPEARSRELRIGSLALPSPVVLAPMAGVTNVAFRTLCRELELATTGTVSGLYVC
CCCCCCCCCCCCCEEECCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHH
EMVTARALAERHPVTLHMTTFSPEESPRSLQLYSVDPETTYRAAKMVVDEGLADHIDMNF
HHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCHHHCCCCC
GCPVPKVTRNGGGAALPYKRRLFGQIVAAAVRATAGTDIPVTVKFRVGIDDDHHTHLDAG
CCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCC
AIAESEGAAAVALHARTASQRYSGTADWSQIAALKNHVTTIPVLGNGDIFDAADAVAMME
CCCCCCCCEEEEEEEHHHHHHCCCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHH
QTGCDGVVIGRGCLGRPWLFAELSAVFNGQTIPAPPNLGQVTVIMRRHAELLVDHFGEDK
HCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCH
ALRDMRKHIAWYLHGFPAGGDIRRALALVSTRAELDTLLEQLDQSAPFPEGGNGPRGRQG
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
SPAKVSLPDGWLNDPDDCTVPSAADVMHSGG
CCCEEECCCCCCCCCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]