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The map label for this gene is kstD [H]

Identifier: 169627725

GI number: 169627725

Start: 620234

End: 621913

Strand: Reverse

Name: kstD [H]

Synonym: MAB_0622c

Alternate gene names: 169627725

Gene position: 621913-620234 (Counterclockwise)

Preceding gene: 169627730

Following gene: 169627724

Centisome position: 12.27

GC content: 65.77

Gene sequence:

>1680_bases
ATGACTGAGCAGGAGTACGACGTCGTCGTCGTCGGGAGTGGTGGTGCCGGCATGGTTGCGGCGCTCACCGCGGCACACAA
CGGGCTGAGCACCGTACTGATCGAAAAGGCCCCCCACTTCGGTGGATCGACGGCCCGCTCCGGCGGTGGCGTCTGGATCC
CCAACAATGAGGTCCTCAAGAAAGCTCGCCAGACCGATACTCCCGAGGCCGCCCGCGAGTACCTCTACAGCATCATCGGC
GACGTCGTTCCGCGCGAGAAGATCGATACCTACCTCGATCGCGGGCCGGAGATGCTCTCATTCGTTCTCGCGAACTCCCC
GCTGAAGCTCGACTGGGTGCCCAACTACTCCGACTACTACCCCGAAGCCCCGGGCGGACGTGCCGCCGGTCGCTCGGTGG
AGCCAAAGCCCTTCGACGCGAAGAAGCTCGGCGACGAGCTGCCCAACTTGGAGCCGCCGTACGGCAAGGTTCCCTTGAAC
GTCGTGGTGCGCCAGCAGGATTACGTCCGGCTCAATCAGCTCAAGCGCCACCCGCGCGGAGTGCTGCGCAGCCTGCGGGT
GGGGGTGCGTACCTTCTGGGCCCAGGGCACCGGTAAGAAACTGGTCGGCATGGGGCGGGCGCTCTCGGCCGCACTGCGTA
TCGGCCTGCGCGACGCCGGAGTGCCGGTCAAGCTCAATACCGCCCTCACCGACCTCTATATCGAGGACGGGACCGTCAGG
GGCGTCTACGTGCGCGAGACCGGGGCTCCCGAAGCCTCCGAACCGACCCTCATCCGGGGGCGTCGCGGCGTCATTCTCGC
CTCCGGCGGTTTCGAGCACAACGAGCAGATGCGCGTGAAGTACCAGCGTGCGCCCATCACCACCGAGTGGACCGTGGGCG
CCAAGGCCAACACCGGTGATGGCATCCTGGCCGCCGAAAAGCTCGGAGCCGCACTGGAGTTCATGGAGGACTCGTGGTGG
GGCCCCACGGTTCCGTTGGTCGGCCGGCCATGGTTCGCGCTGTCCGAGCGCAACTCCCCCGGATCGATCATCGTCAACTC
GTCGGGCAAGCGCTTCATGAACGAGTCGCTGCCCTACGTCGAGGCCACTCACCGGATGTACGGCGGCCAGTACGGCCAGG
GGCCCGGCCCGGGCGAGAACCTGCCTGCGTGGCTCGTCTTCGACCAGGAGTACCGCAACCGTTATATCTTCGCCGGATTG
CAGCCGGGGCAGAAGATTCCAAGTAAGTGGATCGAATCGGGCGTCATCGTCAAGGCCGACACCATTGAGGAATTGGCGAA
GAAGGCGAATCTCCCCGTCGAGGAATTACGCGCCACCATCGAGCGATTCAACGGCTTCGCCCGCAGCGGTGTCGACGAGG
ACTTCGGCCGCGGCAAGAGCGCGTACGACCGCTACTACGGTGACCCCACCAACAAGCCGAACCCCAACCTCGGCGCGCTG
ACCAAGGCTCCGTTCTATGCGGCCAAGATGGTGCCGGGAGACCTGGGCACCAAGGGCGGCGTGCGTACCGACGTCAAGGG
GCGTGTGCTGCGCGACGACAACAGCGTCATCGAAGGCCTTTATGCCGCAGGCAATGTCAGCACCCCGGTGATGGGCCACA
CCTACGCCGGTCCCGGCGCCACCATCGGCCCGGCGATGACGTGGGGCTACCTCGCCGCGCTCGATATCGCCGGGAAGTAG

Upstream 100 bases:

>100_bases
CATGTTTCTGTTTTCACCGCAACATCACAGCGTCCGGGCGATGAAACCACAGGCCAGCGCCGGTAGCGCCAGGCCGCCTC
AATTCTATAACGTGTTCTAC

Downstream 100 bases:

>100_bases
GGTCCGAATATGCCTATCAATGTCGATATCGCGCTGGGCGCGGCTACCGAACCGGCCGAATTTTCGTGGACTGCGTCCGA
TGTGCAGCTGTATCACCTGG

Product: 3-ketosteroid-delta-1-dehydrogenase

Products: NA

Alternate protein names: 3-keto-Delta(4)-steroid Delta(1)-dehydrogenase; KSDD; 3-oxo-Delta(4)-steroid 1-dehydrogenase; KSTD [H]

Number of amino acids: Translated: 559; Mature: 558

Protein sequence:

>559_residues
MTEQEYDVVVVGSGGAGMVAALTAAHNGLSTVLIEKAPHFGGSTARSGGGVWIPNNEVLKKARQTDTPEAAREYLYSIIG
DVVPREKIDTYLDRGPEMLSFVLANSPLKLDWVPNYSDYYPEAPGGRAAGRSVEPKPFDAKKLGDELPNLEPPYGKVPLN
VVVRQQDYVRLNQLKRHPRGVLRSLRVGVRTFWAQGTGKKLVGMGRALSAALRIGLRDAGVPVKLNTALTDLYIEDGTVR
GVYVRETGAPEASEPTLIRGRRGVILASGGFEHNEQMRVKYQRAPITTEWTVGAKANTGDGILAAEKLGAALEFMEDSWW
GPTVPLVGRPWFALSERNSPGSIIVNSSGKRFMNESLPYVEATHRMYGGQYGQGPGPGENLPAWLVFDQEYRNRYIFAGL
QPGQKIPSKWIESGVIVKADTIEELAKKANLPVEELRATIERFNGFARSGVDEDFGRGKSAYDRYYGDPTNKPNPNLGAL
TKAPFYAAKMVPGDLGTKGGVRTDVKGRVLRDDNSVIEGLYAAGNVSTPVMGHTYAGPGATIGPAMTWGYLAALDIAGK

Sequences:

>Translated_559_residues
MTEQEYDVVVVGSGGAGMVAALTAAHNGLSTVLIEKAPHFGGSTARSGGGVWIPNNEVLKKARQTDTPEAAREYLYSIIG
DVVPREKIDTYLDRGPEMLSFVLANSPLKLDWVPNYSDYYPEAPGGRAAGRSVEPKPFDAKKLGDELPNLEPPYGKVPLN
VVVRQQDYVRLNQLKRHPRGVLRSLRVGVRTFWAQGTGKKLVGMGRALSAALRIGLRDAGVPVKLNTALTDLYIEDGTVR
GVYVRETGAPEASEPTLIRGRRGVILASGGFEHNEQMRVKYQRAPITTEWTVGAKANTGDGILAAEKLGAALEFMEDSWW
GPTVPLVGRPWFALSERNSPGSIIVNSSGKRFMNESLPYVEATHRMYGGQYGQGPGPGENLPAWLVFDQEYRNRYIFAGL
QPGQKIPSKWIESGVIVKADTIEELAKKANLPVEELRATIERFNGFARSGVDEDFGRGKSAYDRYYGDPTNKPNPNLGAL
TKAPFYAAKMVPGDLGTKGGVRTDVKGRVLRDDNSVIEGLYAAGNVSTPVMGHTYAGPGATIGPAMTWGYLAALDIAGK
>Mature_558_residues
TEQEYDVVVVGSGGAGMVAALTAAHNGLSTVLIEKAPHFGGSTARSGGGVWIPNNEVLKKARQTDTPEAAREYLYSIIGD
VVPREKIDTYLDRGPEMLSFVLANSPLKLDWVPNYSDYYPEAPGGRAAGRSVEPKPFDAKKLGDELPNLEPPYGKVPLNV
VVRQQDYVRLNQLKRHPRGVLRSLRVGVRTFWAQGTGKKLVGMGRALSAALRIGLRDAGVPVKLNTALTDLYIEDGTVRG
VYVRETGAPEASEPTLIRGRRGVILASGGFEHNEQMRVKYQRAPITTEWTVGAKANTGDGILAAEKLGAALEFMEDSWWG
PTVPLVGRPWFALSERNSPGSIIVNSSGKRFMNESLPYVEATHRMYGGQYGQGPGPGENLPAWLVFDQEYRNRYIFAGLQ
PGQKIPSKWIESGVIVKADTIEELAKKANLPVEELRATIERFNGFARSGVDEDFGRGKSAYDRYYGDPTNKPNPNLGALT
KAPFYAAKMVPGDLGTKGGVRTDVKGRVLRDDNSVIEGLYAAGNVSTPVMGHTYAGPGATIGPAMTWGYLAALDIAGK

Specific function: Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3- ketosteroids. Is also involved in the formation of 3-keto-1,4- diene-steroid from 3-keto-4-ene-steroid [H]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. 3-oxosteroid dehydrogenase subfamily [H]

Homologues:

Organism=Caenorhabditis elegans, GI71986328, Length=347, Percent_Identity=26.5129682997118, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.3.99.4 [H]

Molecular weight: Translated: 60563; Mature: 60432

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQEYDVVVVGSGGAGMVAALTAAHNGLSTVLIEKAPHFGGSTARSGGGVWIPNNEVLK
CCCCCCCEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEECCCHHHHH
KARQTDTPEAAREYLYSIIGDVVPREKIDTYLDRGPEMLSFVLANSPLKLDWVPNYSDYY
HHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHCCCCEEEEECCCCHHCC
PEAPGGRAAGRSVEPKPFDAKKLGDELPNLEPPYGKVPLNVVVRQQDYVRLNQLKRHPRG
CCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHH
VLRSLRVGVRTFWAQGTGKKLVGMGRALSAALRIGLRDAGVPVKLNTALTDLYIEDGTVR
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECEEEEEEEECCEEE
GVYVRETGAPEASEPTLIRGRRGVILASGGFEHNEQMRVKYQRAPITTEWTVGAKANTGD
EEEEECCCCCCCCCCCEEECCCCEEEECCCCCCCCCEEEEEECCCCCEEEECCCCCCCCC
GILAAEKLGAALEFMEDSWWGPTVPLVGRPWFALSERNSPGSIIVNSSGKRFMNESLPYV
CEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCEEEECCCCCHHHHCCCCHH
EATHRMYGGQYGQGPGPGENLPAWLVFDQEYRNRYIFAGLQPGQKIPSKWIESGVIVKAD
HHHHHHHCCCCCCCCCCCCCCCEEEEEEHHHCCEEEEEECCCCHHHHHHHHHCCCEEEHH
TIEELAKKANLPVEELRATIERFNGFARSGVDEDFGRGKSAYDRYYGDPTNKPNPNLGAL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHCCCCCCCCCCCCCCC
TKAPFYAAKMVPGDLGTKGGVRTDVKGRVLRDDNSVIEGLYAAGNVSTPVMGHTYAGPGA
CCCCCCEEECCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCC
TIGPAMTWGYLAALDIAGK
CCCHHHHHHHHHEEECCCC
>Mature Secondary Structure 
TEQEYDVVVVGSGGAGMVAALTAAHNGLSTVLIEKAPHFGGSTARSGGGVWIPNNEVLK
CCCCCCEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEECCCHHHHH
KARQTDTPEAAREYLYSIIGDVVPREKIDTYLDRGPEMLSFVLANSPLKLDWVPNYSDYY
HHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHCCCCEEEEECCCCHHCC
PEAPGGRAAGRSVEPKPFDAKKLGDELPNLEPPYGKVPLNVVVRQQDYVRLNQLKRHPRG
CCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHH
VLRSLRVGVRTFWAQGTGKKLVGMGRALSAALRIGLRDAGVPVKLNTALTDLYIEDGTVR
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECEEEEEEEECCEEE
GVYVRETGAPEASEPTLIRGRRGVILASGGFEHNEQMRVKYQRAPITTEWTVGAKANTGD
EEEEECCCCCCCCCCCEEECCCCEEEECCCCCCCCCEEEEEECCCCCEEEECCCCCCCCC
GILAAEKLGAALEFMEDSWWGPTVPLVGRPWFALSERNSPGSIIVNSSGKRFMNESLPYV
CEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCEEEECCCCCHHHHCCCCHH
EATHRMYGGQYGQGPGPGENLPAWLVFDQEYRNRYIFAGLQPGQKIPSKWIESGVIVKAD
HHHHHHHCCCCCCCCCCCCCCCEEEEEEHHHCCEEEEEECCCCHHHHHHHHHCCCEEEHH
TIEELAKKANLPVEELRATIERFNGFARSGVDEDFGRGKSAYDRYYGDPTNKPNPNLGAL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHCCCCCCCCCCCCCCC
TKAPFYAAKMVPGDLGTKGGVRTDVKGRVLRDDNSVIEGLYAAGNVSTPVMGHTYAGPGA
CCCCCCEEECCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCC
TIGPAMTWGYLAALDIAGK
CCCHHHHHHHHHEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036 [H]