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The map label for this gene is folE

Identifier: 169627638

GI number: 169627638

Start: 536541

End: 537155

Strand: Direct

Name: folE

Synonym: MAB_0534

Alternate gene names: 169627638

Gene position: 536541-537155 (Clockwise)

Preceding gene: 169627637

Following gene: 169627639

Centisome position: 10.59

GC content: 63.25

Gene sequence:

>615_bases
ATGAACTCGCCCGAGACGGCCGAGTACAACGGCCATCCGACCGGTCACGTCTTCGACCAGGCACGTGCCGAGGCCGCGGT
CCGTGAACTGCTCTATGCGGTGGGTGAAGACCCGGATCGGCATGGCTTGGCGGATACACCGGCCCGCGTCGCGCGTGCGT
ACAGGGAGATTTTCGCCGGGCTCTACACCGATCCGGATACCGTGCTGAACACCACCTTCGACGAGCAGCATGACGAGCTG
GTGTTGGTGAAGTCGATACCGATGTACTCGACGTGTGAACATCACCTGGTGTCGTTCCACGGCGTCGCACATGTCGGATA
CATCCCCGGCCAGCACGGCCGCGTCACGGGGCTCTCGAAAATCGCTCGCTTGGTTGACCTTTACGCGAAACGACCGCAGG
TGCAGGAGCGTCTGACAGCGCAGATCGCCGACGCGCTGGTGCGCAAGCTCGAGCCGCGCGGAGTGATCGTCGTGGTGGAA
GCCGAGCATCTGTGCATGGCCATGCGCGGCGTACGCAAGCCCGGTGCGACTACCACGACCTCCGCCGTACGGGGTCAGTT
CAAACGTGACGCCGCTTCCCGGGCCGAGGTTCTTGACTTGATGATGAGGACATGA

Upstream 100 bases:

>100_bases
TATCAGCCATATCCGCCGCAGCCCTACCCGTACCCGGTGCCGACTCCGCATCAGCAGCCGGGCCCGGCTCCGGACGAGGG
CAGCGAGAGCAAGGGCTGAT

Downstream 100 bases:

>100_bases
TCGCTGGCGTGACGGAAACTCCCGCACTGACCCACATCCTGGGAGTCGTCAATGTCACCGACGACTCCTTCTCTGACGGT
GGACGATTCGTCCGTCAATC

Product: GTP cyclohydrolase I (FolE)

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MNSPETAEYNGHPTGHVFDQARAEAAVRELLYAVGEDPDRHGLADTPARVARAYREIFAGLYTDPDTVLNTTFDEQHDEL
VLVKSIPMYSTCEHHLVSFHGVAHVGYIPGQHGRVTGLSKIARLVDLYAKRPQVQERLTAQIADALVRKLEPRGVIVVVE
AEHLCMAMRGVRKPGATTTTSAVRGQFKRDAASRAEVLDLMMRT

Sequences:

>Translated_204_residues
MNSPETAEYNGHPTGHVFDQARAEAAVRELLYAVGEDPDRHGLADTPARVARAYREIFAGLYTDPDTVLNTTFDEQHDEL
VLVKSIPMYSTCEHHLVSFHGVAHVGYIPGQHGRVTGLSKIARLVDLYAKRPQVQERLTAQIADALVRKLEPRGVIVVVE
AEHLCMAMRGVRKPGATTTTSAVRGQFKRDAASRAEVLDLMMRT
>Mature_204_residues
MNSPETAEYNGHPTGHVFDQARAEAAVRELLYAVGEDPDRHGLADTPARVARAYREIFAGLYTDPDTVLNTTFDEQHDEL
VLVKSIPMYSTCEHHLVSFHGVAHVGYIPGQHGRVTGLSKIARLVDLYAKRPQVQERLTAQIADALVRKLEPRGVIVVVE
AEHLCMAMRGVRKPGATTTTSAVRGQFKRDAASRAEVLDLMMRT

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=178, Percent_Identity=50.561797752809, Blast_Score=172, Evalue=2e-43,
Organism=Homo sapiens, GI4503949, Length=178, Percent_Identity=50.561797752809, Blast_Score=172, Evalue=2e-43,
Organism=Homo sapiens, GI66932970, Length=138, Percent_Identity=52.1739130434783, Blast_Score=135, Evalue=3e-32,
Organism=Homo sapiens, GI66932972, Length=138, Percent_Identity=52.1739130434783, Blast_Score=135, Evalue=3e-32,
Organism=Escherichia coli, GI1788476, Length=172, Percent_Identity=41.8604651162791, Blast_Score=113, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17560486, Length=177, Percent_Identity=50.8474576271186, Blast_Score=184, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6321706, Length=184, Percent_Identity=49.4565217391304, Blast_Score=174, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24656782, Length=184, Percent_Identity=48.3695652173913, Blast_Score=159, Evalue=9e-40,
Organism=Drosophila melanogaster, GI24656796, Length=184, Percent_Identity=48.3695652173913, Blast_Score=158, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24656791, Length=184, Percent_Identity=48.3695652173913, Blast_Score=158, Evalue=2e-39,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_MYCA9 (B1MGU9)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001701287.1
- ProteinModelPortal:   B1MGU9
- SMR:   B1MGU9
- EnsemblBacteria:   EBMYCT00000005696
- GeneID:   5963071
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_0534
- GeneTree:   EBGT00050000016683
- HOGENOM:   HBG370191
- OMA:   ARFMSMI
- BioCyc:   MABS561007:MAB_0534-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- TIGRFAMs:   TIGR00063

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 22556; Mature: 22556

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSPETAEYNGHPTGHVFDQARAEAAVRELLYAVGEDPDRHGLADTPARVARAYREIFAG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
LYTDPDTVLNTTFDEQHDELVLVKSIPMYSTCEHHLVSFHGVAHVGYIPGQHGRVTGLSK
HCCCCHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCHHHCCCCCCCCCCHHHHHH
IARLVDLYAKRPQVQERLTAQIADALVRKLEPRGVIVVVEAEHLCMAMRGVRKPGATTTT
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHCCCCCCCCHH
SAVRGQFKRDAASRAEVLDLMMRT
HHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNSPETAEYNGHPTGHVFDQARAEAAVRELLYAVGEDPDRHGLADTPARVARAYREIFAG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
LYTDPDTVLNTTFDEQHDELVLVKSIPMYSTCEHHLVSFHGVAHVGYIPGQHGRVTGLSK
HCCCCHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCHHHCCCCCCCCCCHHHHHH
IARLVDLYAKRPQVQERLTAQIADALVRKLEPRGVIVVVEAEHLCMAMRGVRKPGATTTT
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHCCCCCCCCHH
SAVRGQFKRDAASRAEVLDLMMRT
HHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA