Click here to switch to the map view.

The map label for this gene is ape2 [H]

Identifier: 169627613

GI number: 169627613

Start: 506470

End: 507822

Strand: Reverse

Name: ape2 [H]

Synonym: MAB_0509c

Alternate gene names: 169627613

Gene position: 507822-506470 (Counterclockwise)

Preceding gene: 169627614

Following gene: 169627612

Centisome position: 10.02

GC content: 61.27

Gene sequence:

>1353_bases
ATGACCAAACCCGGCAAAAAGGCAGCCAAACGGCTCCCCCCGCGGCCTCCGGTCATCGATCCCTATCTGCCCCAAAACGG
CAACTACGGGTACCGGGTGTCCCGCTACGAGCTGGACCTCGAATACAAGGTGGCCATCAACCGGCTGACCGGCACCGCGA
ACATCACCGCCGTCACCCTGGCCACCATCAAGGAGTTCACCCTCGACCTCGCCGACACCCTCGGTGTCGCGAAGGTGACA
GTGAACGGTCGACGTCCGACACATTTCGGTCATCGCGCCGGAAAGATCACCATAAAACTGGCATCCGCGATCCCGGCGGG
TGCCGCGATGACCATCGTGATTCGTTATGGCGGGTCACCACGCCCGATCAACAGCATGTGGGGTGATGTCGGATTTGAGG
AGCTGTCCAACGGGGTGCTGGTGGCCGGACAGCCCAACGGTGCGGCATCCTGGTTCCCCTGCGACGACCACCCCAGCTCC
AAGGCGAGCTATCGGTTTGTCATCACCAGCGACAGCCCATATTGCGTTATCTCCAATGGAGAGTTGATATCCCGACGCGT
CCGGGCCGCCCATACCACCTGGACGTACGAACAGCCCGAGCCCATGGCGCCGTATCTGGCGACACTACAGATCGGCGACT
ACCAGCTGGTCAAGCTTGCCAAGAACCCCATCTCGATGAACGCTGCGCTGCCATCACGGCTACGCGATAGGTTCGAGCAC
GACTTCGGCCGACAGCCGCAGATGATGAAGCTGTTCATCAAACAGTTCGGTCCCTATCCGTTCGCCGCGGGATACACCGT
GGTGGTGACCGATGATGACCTCGATATCCCACTGGAGGCACAGGGAATTTCGGTATTCGGCGCCAACCATTGCGACGGTT
CCCGGCATTCAGAACGACTCGTGGCGCACGAGCTGGCCCATCAGTGGTTCGGCAACAGCGTTACCGCGCGCCGCTGGCGC
GACATCTGGTTGCACGAAGGATTTGCCTGTTACGCCGAATGGCTGTGGTCCGAGGAATCCGGCGGGCCGACCGCCGACGC
GCGCGCCCGCCACTACCATCAAAAGCTGAAAGATCTACCGCAAGACCTGATTCTGGCCGATCCCGGCCCACAACTGATGT
TCGACGATCGCGTATACAAGCGCGGAGCACTGACATTGCACGCCCTGCGATTGCGCCTCGGTGATACGAAATTCTTTGCA
CTGCTGCAGGACTGGACCACCCGATATCGGCACAGCACCGTCATTACCGACGACTTCACCGGCCTGGCCGCCGGGTACTC
CGATGAATCCCTGCGCCCGCTGTGGACGGCATGGCTCTACGAGAAGCAGCTCCCGCCCTTGGACCGGAAGTGA

Upstream 100 bases:

>100_bases
GGCAGGGCAATCCCATTTCGCCATGGGGCAAGCTGGATCCGATGGCACCGAAGAAGCCCAAGAGCGGCAAGGTCTCCCCA
AACAAGAATTCCGGGGCCGC

Downstream 100 bases:

>100_bases
CCGACGCCGGTCCCAGCACCCGCTCCAGCGTCGCGCGCATCGGTGCCGCCACCGCCGTCAGCGCGATCTGCGGATACGCC
GTGCTGTACCTGGCAGCCCG

Product: peptidase M1, membrane alanine aminopeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MTKPGKKAAKRLPPRPPVIDPYLPQNGNYGYRVSRYELDLEYKVAINRLTGTANITAVTLATIKEFTLDLADTLGVAKVT
VNGRRPTHFGHRAGKITIKLASAIPAGAAMTIVIRYGGSPRPINSMWGDVGFEELSNGVLVAGQPNGAASWFPCDDHPSS
KASYRFVITSDSPYCVISNGELISRRVRAAHTTWTYEQPEPMAPYLATLQIGDYQLVKLAKNPISMNAALPSRLRDRFEH
DFGRQPQMMKLFIKQFGPYPFAAGYTVVVTDDDLDIPLEAQGISVFGANHCDGSRHSERLVAHELAHQWFGNSVTARRWR
DIWLHEGFACYAEWLWSEESGGPTADARARHYHQKLKDLPQDLILADPGPQLMFDDRVYKRGALTLHALRLRLGDTKFFA
LLQDWTTRYRHSTVITDDFTGLAAGYSDESLRPLWTAWLYEKQLPPLDRK

Sequences:

>Translated_450_residues
MTKPGKKAAKRLPPRPPVIDPYLPQNGNYGYRVSRYELDLEYKVAINRLTGTANITAVTLATIKEFTLDLADTLGVAKVT
VNGRRPTHFGHRAGKITIKLASAIPAGAAMTIVIRYGGSPRPINSMWGDVGFEELSNGVLVAGQPNGAASWFPCDDHPSS
KASYRFVITSDSPYCVISNGELISRRVRAAHTTWTYEQPEPMAPYLATLQIGDYQLVKLAKNPISMNAALPSRLRDRFEH
DFGRQPQMMKLFIKQFGPYPFAAGYTVVVTDDDLDIPLEAQGISVFGANHCDGSRHSERLVAHELAHQWFGNSVTARRWR
DIWLHEGFACYAEWLWSEESGGPTADARARHYHQKLKDLPQDLILADPGPQLMFDDRVYKRGALTLHALRLRLGDTKFFA
LLQDWTTRYRHSTVITDDFTGLAAGYSDESLRPLWTAWLYEKQLPPLDRK
>Mature_449_residues
TKPGKKAAKRLPPRPPVIDPYLPQNGNYGYRVSRYELDLEYKVAINRLTGTANITAVTLATIKEFTLDLADTLGVAKVTV
NGRRPTHFGHRAGKITIKLASAIPAGAAMTIVIRYGGSPRPINSMWGDVGFEELSNGVLVAGQPNGAASWFPCDDHPSSK
ASYRFVITSDSPYCVISNGELISRRVRAAHTTWTYEQPEPMAPYLATLQIGDYQLVKLAKNPISMNAALPSRLRDRFEHD
FGRQPQMMKLFIKQFGPYPFAAGYTVVVTDDDLDIPLEAQGISVFGANHCDGSRHSERLVAHELAHQWFGNSVTARRWRD
IWLHEGFACYAEWLWSEESGGPTADARARHYHQKLKDLPQDLILADPGPQLMFDDRVYKRGALTLHALRLRLGDTKFFAL
LQDWTTRYRHSTVITDDFTGLAAGYSDESLRPLWTAWLYEKQLPPLDRK

Specific function: Preferentially acts as a leucyl-aminopeptidase, although it also has activity against other substrates [H]

COG id: COG0308

COG function: function code E; Aminopeptidase N

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M1 family [H]

Homologues:

Organism=Homo sapiens, GI4505029, Length=447, Percent_Identity=22.8187919463087, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI61742775, Length=382, Percent_Identity=23.5602094240838, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI61742777, Length=382, Percent_Identity=23.5602094240838, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI94818891, Length=355, Percent_Identity=24.7887323943662, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI94818901, Length=355, Percent_Identity=24.7887323943662, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI132814467, Length=336, Percent_Identity=26.4880952380952, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI158937236, Length=318, Percent_Identity=23.2704402515723, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI194306629, Length=297, Percent_Identity=23.5690235690236, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI11641261, Length=297, Percent_Identity=23.5690235690236, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI310123622, Length=290, Percent_Identity=24.4827586206897, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI40316915, Length=306, Percent_Identity=24.5098039215686, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI310133497, Length=290, Percent_Identity=24.1379310344828, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI157266300, Length=207, Percent_Identity=28.9855072463768, Blast_Score=69, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI71984339, Length=471, Percent_Identity=24.6284501061571, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71984353, Length=472, Percent_Identity=24.7881355932203, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17544504, Length=400, Percent_Identity=24, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17565628, Length=197, Percent_Identity=30.9644670050761, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17569225, Length=187, Percent_Identity=31.0160427807487, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI133903840, Length=314, Percent_Identity=25.1592356687898, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17569221, Length=205, Percent_Identity=28.780487804878, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71989071, Length=326, Percent_Identity=22.0858895705521, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71989076, Length=326, Percent_Identity=22.0858895705521, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI9755335, Length=333, Percent_Identity=24.3243243243243, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6321837, Length=330, Percent_Identity=23.3333333333333, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324283, Length=330, Percent_Identity=25.1515151515152, Blast_Score=73, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24646514, Length=337, Percent_Identity=27.893175074184, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24646518, Length=337, Percent_Identity=27.893175074184, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24646516, Length=337, Percent_Identity=27.893175074184, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24646512, Length=390, Percent_Identity=24.1025641025641, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24646510, Length=390, Percent_Identity=24.1025641025641, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24651016, Length=280, Percent_Identity=26.0714285714286, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24651025, Length=329, Percent_Identity=24.0121580547112, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24651023, Length=329, Percent_Identity=24.0121580547112, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24651021, Length=329, Percent_Identity=24.0121580547112, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24585062, Length=425, Percent_Identity=23.7647058823529, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24585064, Length=425, Percent_Identity=23.7647058823529, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24585066, Length=425, Percent_Identity=23.7647058823529, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI45550350, Length=425, Percent_Identity=23.7647058823529, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21358341, Length=298, Percent_Identity=25.503355704698, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24650973, Length=237, Percent_Identity=28.2700421940928, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI45550850, Length=237, Percent_Identity=28.2700421940928, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI161078673, Length=348, Percent_Identity=22.7011494252874, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI28571901, Length=348, Percent_Identity=22.7011494252874, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24655257, Length=384, Percent_Identity=22.3958333333333, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24655274, Length=384, Percent_Identity=22.3958333333333, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24655260, Length=384, Percent_Identity=22.3958333333333, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24655265, Length=384, Percent_Identity=22.3958333333333, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24655268, Length=384, Percent_Identity=22.3958333333333, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24655252, Length=385, Percent_Identity=21.8181818181818, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001930
- InterPro:   IPR014782 [H]

Pfam domain/function: PF01433 Peptidase_M1 [H]

EC number: =3.4.11.1 [H]

Molecular weight: Translated: 50514; Mature: 50383

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPGKKAAKRLPPRPPVIDPYLPQNGNYGYRVSRYELDLEYKVAINRLTGTANITAVTL
CCCCCHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEEEEEEEECCCCCEEEEEH
ATIKEFTLDLADTLGVAKVTVNGRRPTHFGHRAGKITIKLASAIPAGAAMTIVIRYGGSP
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEEECCCC
RPINSMWGDVGFEELSNGVLVAGQPNGAASWFPCDDHPSSKASYRFVITSDSPYCVISNG
CCCHHHHCCCCHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEECC
ELISRRVRAAHTTWTYEQPEPMAPYLATLQIGDYQLVKLAKNPISMNAALPSRLRDRFEH
HHHHHHHHHHHCEECCCCCCCCCCEEEEEEECCEEEEEECCCCCCCCCCCHHHHHHHHHH
DFGRQPQMMKLFIKQFGPYPFAAGYTVVVTDDDLDIPLEAQGISVFGANHCDGSRHSERL
HCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEECCEEEEECCCCCCCHHHHHH
VAHELAHQWFGNSVTARRWRDIWLHEGFACYAEWLWSEESGGPTADARARHYHQKLKDLP
HHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCC
QDLILADPGPQLMFDDRVYKRGALTLHALRLRLGDTKFFALLQDWTTRYRHSTVITDDFT
HHEEEECCCCCEEECCHHHHCCCCEEEEEHHHCCCHHHHHHHHHHHHHHHCCEEEEECCC
GLAAGYSDESLRPLWTAWLYEKQLPPLDRK
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TKPGKKAAKRLPPRPPVIDPYLPQNGNYGYRVSRYELDLEYKVAINRLTGTANITAVTL
CCCCHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEEEEEEEECCCCCEEEEEH
ATIKEFTLDLADTLGVAKVTVNGRRPTHFGHRAGKITIKLASAIPAGAAMTIVIRYGGSP
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEEECCCC
RPINSMWGDVGFEELSNGVLVAGQPNGAASWFPCDDHPSSKASYRFVITSDSPYCVISNG
CCCHHHHCCCCHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEECC
ELISRRVRAAHTTWTYEQPEPMAPYLATLQIGDYQLVKLAKNPISMNAALPSRLRDRFEH
HHHHHHHHHHHCEECCCCCCCCCCEEEEEEECCEEEEEECCCCCCCCCCCHHHHHHHHHH
DFGRQPQMMKLFIKQFGPYPFAAGYTVVVTDDDLDIPLEAQGISVFGANHCDGSRHSERL
HCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEECCEEEEECCCCCCCHHHHHH
VAHELAHQWFGNSVTARRWRDIWLHEGFACYAEWLWSEESGGPTADARARHYHQKLKDLP
HHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCC
QDLILADPGPQLMFDDRVYKRGALTLHALRLRLGDTKFFALLQDWTTRYRHSTVITDDFT
HHEEEECCCCCEEECCHHHHCCCCEEEEEHHHCCCHHHHHHHHHHHHHHHCCEEEEECCC
GLAAGYSDESLRPLWTAWLYEKQLPPLDRK
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9723917; 8899719; 11427726 [H]