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The map label for this gene is leuA [H]

Identifier: 169627441

GI number: 169627441

Start: 334320

End: 336143

Strand: Reverse

Name: leuA [H]

Synonym: MAB_0337c

Alternate gene names: 169627441

Gene position: 336143-334320 (Counterclockwise)

Preceding gene: 169627442

Following gene: 169627438

Centisome position: 6.63

GC content: 62.72

Gene sequence:

>1824_bases
ATGACTAGCTTCTCCTCAGACGAGTTCAACTACACCCCTCGCAGCGTCGTTACCCCGAATGGACCCATTCCCGCCGATCA
GCCGGCATGGAACACCCAGAAGAACAGCTCGATGCCGGTACACCGGTACCGCTCTTTCACCGACGAGGTCGAAAAGATCG
AGCTGCCGGACCGCACCTGGCCCGACAAGGTAATTACCCGTGCACCGCAGTGGGGCGCCGTCGACCTGCGCGACGGCAAC
CAGGCCTTGATCGATCCGATGAGTCCCGCCCGCAAGCGCCGGATGTTCGATCTGCAGATCCGGATGGGCTTCAAGGAGAT
CGAAGTCGGTTTCCCGTCGGCCAGCCAGACCGATTTCGATTTCATCCGCGAGCTGATCACCGAGAACGCGATTCCCGACG
ATGTCACCATCCAGGTGCTGACCCAGTGCCGTCCGGAGCTGATCGAGCGGACGTTCCTGGCGTGTGAGGGCGCGTCCAAG
GTGATCGTGCACTTTTACAACTCGACCTCGATCCTGCAGCGGCGGGTCGTGTTCCGCGAAGACAAGGACGCGATCAAGAA
GATCGCCACCGACGGCGCACACAAATGCGTCGAAGAAGCCGCGAAATATCCCGACACCCATTGGCGTTTCCAGTACTCCC
CCGAGTCGTACACCGGGACCGAGCTGCAGTACGCGCTGGAGGTGTGTAATGCCGTCGGCGATATCGTCGAGCCGACGCCG
GACAATCCGATCATCTTCAACCTGCCGTCGACGGTGGAGATGACCACCCCCAACGTGTACGCCGACTCCATCGAGTGGAT
GAGCCGCAATCTGGCCCGCCGCGATTCGGTGATCCTGAGCCTGCATCCGCATAACGACCGCGGAACAGCGGTCGCCGCAG
CGGAGTTGGGCTTCCAGGCGGGCGCGGACCGCATCGAGGGCTGTCTGTTCGGCAACGGTGAGCGCACCGGAAACGTCTGC
CTGGTAACGCTGGGACTGAACCTGTTCAGTCGAGGCGTCGACCCACAGATCGACTTCTCGAACATCGATGAGATCCGGCA
CACGGTCGAGTACTGCAACCAGCTGAAGGTGCCCGAGCGCCACCCGTACGGTGGCGATCTGGTCTACACCGCCTTCTCCG
GCAGTCACCAGGACGCCATCAATAAAGGCCTGGATCAGATGAAACGCGATGCAGACGAAGCAGATTCGGATGTCGATGAC
ATCCTGTGGCAGGTGCCGTATCTGCCCATCGATCCCAAGGATGTCGGGCGCAACTACGAGGCCGTGATCCGCGTCAACTC
GCAGTCGGGCAAGGGTGGTGTCGCCTACATCATGAAGTCCGATCATGGACTGGTGCTGCCGCGCCGTCTGCAGATCGAGT
TCGCACAGTCGATCCAGCGCATCACCGACGGTGAGGGCGGCGAGGTGAACCCCAAGGAGATCTGGGATGTCTTCGCCGAG
GAGTACCTGGCACCAATCACCCCGCTGGAGCGCATTCGCCAGAAGGTGACCGCCTCCGAAGAGGACGGTGGCACGGATTC
CATTGCCGCCGTGGTGAAGATCGACGGCAAGGAAACCGAGGTCAACGGCACGGGTAACGGTCCGTTGGCAGCTTTCGTGG
ACGCCCTGTCCACCGTGGGCTACACCGTCAACGTGTTGGACTACTCCGAGCACGCGCTGAGCGCAGGCGACGACGCGCAG
GCCGCCGCCTACGTGGAGGCCACGGTTGTCAGCCCCGGCGGCGGTGAGGCGCAGACCGTCTGGGGTGTCGGCATCGCGCC
GTCGATCACCACCGCCTCGCTGCGGGCAGTGGTGTCGGCGGTCAACCGTGCTGCTCGCGCCTAG

Upstream 100 bases:

>100_bases
CTCCTCCGACGCCGCGACAGGGTCTGATCCAGACCGGCTTCCTGTCGCGGGTTGTTGGCGTGCCGGTCGAGATCCCCATT
CAAACTCCCGGAGCCTTCCC

Downstream 100 bases:

>100_bases
TCCCTTTCAGCGAAAGCGACCCTGGTGCGGAATTCCCTCGGCATTCCGCACCAGGGTCGCACCCGACATGGCTATCTAGT
CCCGTAGCGCCTGCGGAACT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 607; Mature: 606

Protein sequence:

>607_residues
MTSFSSDEFNYTPRSVVTPNGPIPADQPAWNTQKNSSMPVHRYRSFTDEVEKIELPDRTWPDKVITRAPQWGAVDLRDGN
QALIDPMSPARKRRMFDLQIRMGFKEIEVGFPSASQTDFDFIRELITENAIPDDVTIQVLTQCRPELIERTFLACEGASK
VIVHFYNSTSILQRRVVFREDKDAIKKIATDGAHKCVEEAAKYPDTHWRFQYSPESYTGTELQYALEVCNAVGDIVEPTP
DNPIIFNLPSTVEMTTPNVYADSIEWMSRNLARRDSVILSLHPHNDRGTAVAAAELGFQAGADRIEGCLFGNGERTGNVC
LVTLGLNLFSRGVDPQIDFSNIDEIRHTVEYCNQLKVPERHPYGGDLVYTAFSGSHQDAINKGLDQMKRDADEADSDVDD
ILWQVPYLPIDPKDVGRNYEAVIRVNSQSGKGGVAYIMKSDHGLVLPRRLQIEFAQSIQRITDGEGGEVNPKEIWDVFAE
EYLAPITPLERIRQKVTASEEDGGTDSIAAVVKIDGKETEVNGTGNGPLAAFVDALSTVGYTVNVLDYSEHALSAGDDAQ
AAAYVEATVVSPGGGEAQTVWGVGIAPSITTASLRAVVSAVNRAARA

Sequences:

>Translated_607_residues
MTSFSSDEFNYTPRSVVTPNGPIPADQPAWNTQKNSSMPVHRYRSFTDEVEKIELPDRTWPDKVITRAPQWGAVDLRDGN
QALIDPMSPARKRRMFDLQIRMGFKEIEVGFPSASQTDFDFIRELITENAIPDDVTIQVLTQCRPELIERTFLACEGASK
VIVHFYNSTSILQRRVVFREDKDAIKKIATDGAHKCVEEAAKYPDTHWRFQYSPESYTGTELQYALEVCNAVGDIVEPTP
DNPIIFNLPSTVEMTTPNVYADSIEWMSRNLARRDSVILSLHPHNDRGTAVAAAELGFQAGADRIEGCLFGNGERTGNVC
LVTLGLNLFSRGVDPQIDFSNIDEIRHTVEYCNQLKVPERHPYGGDLVYTAFSGSHQDAINKGLDQMKRDADEADSDVDD
ILWQVPYLPIDPKDVGRNYEAVIRVNSQSGKGGVAYIMKSDHGLVLPRRLQIEFAQSIQRITDGEGGEVNPKEIWDVFAE
EYLAPITPLERIRQKVTASEEDGGTDSIAAVVKIDGKETEVNGTGNGPLAAFVDALSTVGYTVNVLDYSEHALSAGDDAQ
AAAYVEATVVSPGGGEAQTVWGVGIAPSITTASLRAVVSAVNRAARA
>Mature_606_residues
TSFSSDEFNYTPRSVVTPNGPIPADQPAWNTQKNSSMPVHRYRSFTDEVEKIELPDRTWPDKVITRAPQWGAVDLRDGNQ
ALIDPMSPARKRRMFDLQIRMGFKEIEVGFPSASQTDFDFIRELITENAIPDDVTIQVLTQCRPELIERTFLACEGASKV
IVHFYNSTSILQRRVVFREDKDAIKKIATDGAHKCVEEAAKYPDTHWRFQYSPESYTGTELQYALEVCNAVGDIVEPTPD
NPIIFNLPSTVEMTTPNVYADSIEWMSRNLARRDSVILSLHPHNDRGTAVAAAELGFQAGADRIEGCLFGNGERTGNVCL
VTLGLNLFSRGVDPQIDFSNIDEIRHTVEYCNQLKVPERHPYGGDLVYTAFSGSHQDAINKGLDQMKRDADEADSDVDDI
LWQVPYLPIDPKDVGRNYEAVIRVNSQSGKGGVAYIMKSDHGLVLPRRLQIEFAQSIQRITDGEGGEVNPKEIWDVFAEE
YLAPITPLERIRQKVTASEEDGGTDSIAAVVKIDGKETEVNGTGNGPLAAFVDALSTVGYTVNVLDYSEHALSAGDDAQA
AAYVEATVVSPGGGEAQTVWGVGIAPSITTASLRAVVSAVNRAARA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=542, Percent_Identity=24.7232472324723, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324225, Length=570, Percent_Identity=46.3157894736842, Blast_Score=511, Evalue=1e-145,
Organism=Saccharomyces cerevisiae, GI6324682, Length=575, Percent_Identity=45.3913043478261, Blast_Score=503, Evalue=1e-143,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 66846; Mature: 66715

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSFSSDEFNYTPRSVVTPNGPIPADQPAWNTQKNSSMPVHRYRSFTDEVEKIELPDRTW
CCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEECCCCCCC
PDKVITRAPQWGAVDLRDGNQALIDPMSPARKRRMFDLQIRMGFKEIEVGFPSASQTDFD
CHHHHHCCCCCCEEEECCCCEEEECCCCHHHHCCEEEEEEECCCEEEEECCCCCCCCCHH
FIRELITENAIPDDVTIQVLTQCRPELIERTFLACEGASKVIVHFYNSTSILQRRVVFRE
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHC
DKDAIKKIATDGAHKCVEEAAKYPDTHWRFQYSPESYTGTELQYALEVCNAVGDIVEPTP
CHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCC
DNPIIFNLPSTVEMTTPNVYADSIEWMSRNLARRDSVILSLHPHNDRGTAVAAAELGFQA
CCCEEEECCCCEEECCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEHHHHCHHC
GADRIEGCLFGNGERTGNVCLVTLGLNLFSRGVDPQIDFSNIDEIRHTVEYCNQLKVPER
CHHHHCEEEECCCCCCCCEEEEEECHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
HPYGGDLVYTAFSGSHQDAINKGLDQMKRDADEADSDVDDILWQVPYLPIDPKDVGRNYE
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHCCCEE
AVIRVNSQSGKGGVAYIMKSDHGLVLPRRLQIEFAQSIQRITDGEGGEVNPKEIWDVFAE
EEEEEECCCCCCCEEEEEECCCCEECCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
EYLAPITPLERIRQKVTASEEDGGTDSIAAVVKIDGKETEVNGTGNGPLAAFVDALSTVG
HHCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEECCCEEEECCCCCCHHHHHHHHHHHCC
YTVNVLDYSEHALSAGDDAQAAAYVEATVVSPGGGEAQTVWGVGIAPSITTASLRAVVSA
CEEEEEECCHHHCCCCCCCCCEEEEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHH
VNRAARA
HHHHHCC
>Mature Secondary Structure 
TSFSSDEFNYTPRSVVTPNGPIPADQPAWNTQKNSSMPVHRYRSFTDEVEKIELPDRTW
CCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEECCCCCCC
PDKVITRAPQWGAVDLRDGNQALIDPMSPARKRRMFDLQIRMGFKEIEVGFPSASQTDFD
CHHHHHCCCCCCEEEECCCCEEEECCCCHHHHCCEEEEEEECCCEEEEECCCCCCCCCHH
FIRELITENAIPDDVTIQVLTQCRPELIERTFLACEGASKVIVHFYNSTSILQRRVVFRE
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHC
DKDAIKKIATDGAHKCVEEAAKYPDTHWRFQYSPESYTGTELQYALEVCNAVGDIVEPTP
CHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCC
DNPIIFNLPSTVEMTTPNVYADSIEWMSRNLARRDSVILSLHPHNDRGTAVAAAELGFQA
CCCEEEECCCCEEECCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEHHHHCHHC
GADRIEGCLFGNGERTGNVCLVTLGLNLFSRGVDPQIDFSNIDEIRHTVEYCNQLKVPER
CHHHHCEEEECCCCCCCCEEEEEECHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
HPYGGDLVYTAFSGSHQDAINKGLDQMKRDADEADSDVDDILWQVPYLPIDPKDVGRNYE
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHCCCEE
AVIRVNSQSGKGGVAYIMKSDHGLVLPRRLQIEFAQSIQRITDGEGGEVNPKEIWDVFAE
EEEEEECCCCCCCEEEEEECCCCEECCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
EYLAPITPLERIRQKVTASEEDGGTDSIAAVVKIDGKETEVNGTGNGPLAAFVDALSTVG
HHCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEECCCEEEECCCCCCHHHHHHHHHHHCC
YTVNVLDYSEHALSAGDDAQAAAYVEATVVSPGGGEAQTVWGVGIAPSITTASLRAVVSA
CEEEEEECCHHHCCCCCCCCCEEEEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHH
VNRAARA
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]